LOCUS       BBa_J23118_GCmR         4487 bp ds-DNA     circular     13-JUN-2017
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     118-PROPS Example Construct version 1 (still working out some kinks
            while comparing to sequencing)
COMMENT     This is an example construct using the backbone (BBa_J61002) +
            promoter (BBa_J23100) + RBS-PROPS-TERMINATOR  (all three components
            were taken from pBAD)
COMMENT     Question: Am I missing some of the nts that were rescued when the
            promoter was cloned into this backbone (cloned between XBA1/SPE1? I
            just pasted the promoter in but that may not actually be accurate
COMMENT     If this is the case, it would not really matter for expression but
            it could be confusing and problematic using traditional restriction
            digest/ligation cloning. I should compare some sequencing results
            to the backbone.
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     rep_origin      complement(2963..3645)
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(3743..4402)
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1827..2537
                     /label=mRuby3
                     /ApEinfo_fwdcolor=#ff2440
                     /ApEinfo_revcolor=#ff2440
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    412..428
                     /label=RBS (T7)
                     /ApEinfo_fwdcolor=#800080
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2862..2881
                     /label=Reverse Sequencing Primer Binding Site (VR)
                     /ApEinfo_fwdcolor=#8000ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2646..2803
                     /label=rrnB_terminator
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    411..411
                     /label=SpeI digest relic nt
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#004080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2804..2804
                     /label=PstI digest relic nt
                     /ApEinfo_fwdcolor=#0080c0
                     /ApEinfo_revcolor=#ff0080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    236..255
                     /label=Forward Sequencing Primer Site (VF2)
                     /ApEinfo_fwdcolor=#0080ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    411..411
                     /label=Insert Goes between these two nt, include RBS and T
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#800040
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2804..2804
                     /label=Insert Goes between these two nt, include RBS and
                     T(1)
                     /ApEinfo_label=Insert Goes between these two nt, include
                     RBS and T
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#800040
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    369..374
                     /label=XBA1 site, 3' end of promoter insert site
                     /ApEinfo_fwdcolor=#8080c0
                     /ApEinfo_revcolor=#000080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    376..410
                     /label=BBa_J23118 Strong Constituative
                     /ApEinfo_fwdcolor=#235afa
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    441..1790
                     /label=GCaMP6F
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
       61 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
      121 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
      181 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
      241 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
      301 aggcagaatt tcagataaaa aaaatcctta gctttcgcta aggatgattt ctggaattcg
      361 cggccgcatc tagagttgac ggctagctca gtcctaggta ttgtgctagc aTTAACTTTA
      421 AGAAGGAGAT ATACATACCC ATGGGTTCTC ATCATCATCA TCATCATGGT ATGGCTAGCA
      481 TGACTGGTGG ACAGCAAATG GGTCGGGATC TGTACGACGA TGACGATAAG GATCTCGCCA
      541 CCATGGTCGA CTCATCACGT CGTAAGTGGA ATAAGACAGG TCACGCAGTC AGAGCTATAG
      601 GTCGGCTGAG CTCACTCGAG AACGTCTATA TCAAGGCCGA CAAGCAGAAG AACGGCATCA
      661 AGGCGAACTT CAAGATCCGC CACAACATCG AGGACGGCGG CGTGCAGCTC GCCTACCACT
      721 ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA
      781 GCGTGCAGTC CAAACTTTCG AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG
      841 AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG GGCGGTACCG
      901 GAGGGAGCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG
      961 AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGT GAGGGCGATG
     1021 CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT
     1081 GGCCCACCCT CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC
     1141 ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACATC CAGGAGCGCA
     1201 CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG
     1261 ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC
     1321 TGGGGCACAA GCTGGAGTAC AACCTGCCGG ACCAACTGAC TGAAGAGCAG ATCGCAGAAT
     1381 TTAAAGAGGA ATTCTCCCTA TTTGACAAGG ACGGGGATGG GACAATAACA ACCAAGGAGC
     1441 TGGGGACGGT GATGCGGTCT CTGGGGCAGA ACCCCACAGA AGCAGAGCTG CAGGACATGA
     1501 TCAATGAAGT AGATGCCGAC GGTGACGGCA CAATCGACTT CCCTGAGTTC CTGACAATGA
     1561 TGGCAAGAAA AATGAAATAC AGGGACACGG AAGAAGAAAT TAGAGAAGCG TTCGGTGTGT
     1621 TTGATAAGGA TGGCAATGGC TACATCAGTG CAGCAGAGCT TCGCCACGTG ATGACAAACC
     1681 TTGGAGAGAA GTTAACAGAT GAAGAGGTTG ATGAAATGAT CAGGGAAGCA GACATCGATG
     1741 GGGATGGTCA GGTAAACTAC GAAGAGTTTG TACAAATGAT GACAGCGAAG ggtGGATCCg
     1801 gtggtagcgg tggtagcggt GGATCCATGG TGTCTAAGGG CGAAGAGCTG ATCAAGGAAA
     1861 ATATGCGTAT GAAGGTGGTC ATGGAAGGTT CGGTCAACGG CCACCAATTC AAATGCACAG
     1921 GTGAAGGAGA AGGCAGACCG TACGAGGGAA CTCAAACCAT GAGGATCAAA GTCATCGAGG
     1981 GAGGACCCCT GCCATTTGCC TTTGACATTC TTGCCACGTC GTTCATGTAT GGCAGCCGTA
     2041 CTTTTATCAA GTACCCGGCC GACATCCCTG ATTTCTTTAA ACAGTCCTTT CCTGAGGGTT
     2101 TTACTTGGGA AAGAGTTACG AGATACGAAG ATGGTGGAGT CGTCACCGTC ACGCAGGACA
     2161 CCAGCCTTGA GGATGGCGAG CTCGTCTACA ACGTCAAGGT CAGAGGGGTA AACTTTCCCT
     2221 CCAATGGTCC CGTGATGCAG AAGAAGACCA AGGGTTGGGA GCCTAATACA GAGATGATGT
     2281 ATCCAGCAGA TGGTGGTCTG AGAGGATACA CTGACATCGC ACTGAAAGTT GATGGTGGTG
     2341 GCCATCTGCA CTGCAACTTC GTGACAACTT ACAGGTCAAA AAAGACCGTC GGGAACATCA
     2401 AGATGCCCGG TGTCCATGCC GTTGATCACC GCCTGGAAAG GATCGAGGAG AGTGACAATG
     2461 AAACCTACGT AGTGCAACGC GAAGTGGCAG TTGCCAAATA CAGCAACCTT GGTGGTGGCA
     2521 TGGACGAGCT GTACAAGTAA TAAGTTTAAA CGGTCTCCAG CTTGGCTGTT TTGGCGGATG
     2581 AGAGAAGATT TTCAGCCTGA TACAGATTAA ATCAGAACGC AGAAGCGGTC TGATAAAACA
     2641 GAATTTGCCT GGCGGCAGTA GCGCGGTGGT CCCACCTGAC CCCATGCCGA ACTCAGAAGT
     2701 GAAACGCCGT AGCGCCGATG GTAGTGTGGG GTCTCCCCAT GCGAGAGTAG GGAACTGCCA
     2761 GGCATCAAAT AAAACGAAAG GCTCAGTCGA AAGACTGGGC CTTggcttcc tcgctcactg
     2821 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     2881 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     2941 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     3001 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     3061 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     3121 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     3181 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     3241 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     3301 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     3361 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     3421 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     3481 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     3541 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     3601 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     3661 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     3721 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     3781 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     3841 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     3901 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     3961 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     4021 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     4081 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     4141 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     4201 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     4261 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     4321 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     4381 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     4441 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcaccc 
//
