LOCUS       pJMK090___petDUE        7451 bp ds-DNA     circular     13-JUN-2017
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     964..983
                     /label=T7
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    107..142
                     /label=GPR D97N
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#004080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      4105..4787
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      2594..2900
                     /label=F1 ori
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1053..2375
                     /label=GCaMP6F
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    143..853
                     /label=GPR D97N(1)
                     /ApEinfo_label=GPR D97N
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=#004080
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      2577..3032
                     /label=M13 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_binding    3..25
                     /label=LacO
                     /ApEinfo_fwdcolor=#6495ed
                     /ApEinfo_revcolor=#6495ed
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_binding    983..1005
                     /label=LacO(1)
                     /ApEinfo_label=LacO
                     /ApEinfo_fwdcolor=#6495ed
                     /ApEinfo_revcolor=#6495ed
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(5965..6921)
                     /label=LacI
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(3150..3809)
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatatacc atgggcagca gccatcacca tcatcaccac agccagGGTA AATTATTACT
      121 GATATTAGGT AGTGTTATTG CACTTCCTAC ATTTGCTGCA GGTGGTGGTG ACCTTGATGC
      181 TAGTGATTAC ACTGGTGTTT CTTTTTGGTT AGTTACTGCT GCTTTATTAG CATCTACTGT
      241 ATTTTTCTTT GTTGAAAGAG ATAGAGTTTC TGCAAAATGG AAAACATCAT TAACTGTATC
      301 TGGTCTTGTT ACTGGTATTG CTTTCTGGCA TTACATGTAC ATGAGAGGGG TATGGATTGA
      361 AACTGGTGAT TCGCCAACTG TATTTAGATA CATTAATTGG TTACTAACAG TTCCTCTATT
      421 AATATGTGAA TTCTACTTAA TTCTTGCTGC TGCAACTAAT GTTGCTGGAT CATTATTTAA
      481 GAAATTACTA GTTGGTTCTC TTGTTATGCT TGTGTTTGGT TACATGGGTG AAGCAGGAAT
      541 CATGGCTGCA TGGCCTGCAT TCATTATTGG GTGTTTAGCT TGGGTATACA TGATTTATGA
      601 ATTATGGGCT GGAGAAGGAA AATCTGCATG TAATACTGCA AGTCCTGCTG TGCAATCAGC
      661 TTACAACACA ATGATGTATA TTATCATCTT TGGTTGGGCG ATTTATCCTG TAGGTTATTT
      721 CACAGGTTAC CTGATGGGTG ACGGTGGATC AGCTCTTAAC TTAAACCTTA TCTATAACCT
      781 TGCTGACTTT GTTAACAAGA TTCTATTTGG TTTAATTATA TGGAATGTTG CTGTTAAAGA
      841 ATCTTCTAAT GCTTGAgatc cgaattcgag ctcggcgcgc ctgcaggtcg acaagcttgc
      901 ggccgcataa tgcttaagtc gaacagaaag taatcgtatt gtacacggcc gcataatcga
      961 aattaatacg actcactata ggggaattgt gagcggataa caattcccca tcttagtata
     1021 ttagttaagt ataagaagga gatatacata tgGGTATGGC TAGCATGACT GGTGGACAGC
     1081 AAATGGGTCG GGATCTGTAC GACGATGACG ATAAGGATCT CGCCACCATG GTCGACTCAT
     1141 CACGTCGTAA GTGGAATAAG ACAGGTCACG CAGTCAGAGC TATAGGTCGG CTGAGCTCAC
     1201 TCGAGAACGT CTATATCAAG GCCGACAAGC AGAAGAACGG CATCAAGGCG AACTTCAAGA
     1261 TCCGCCACAA CATCGAGGAC GGCGGCGTGC AGCTCGCCTA CCACTACCAG CAGAACACCC
     1321 CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCGTG CAGTCCAAAC
     1381 TTTCGAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG
     1441 CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGGGCGG TACCGGAGGG AGCATGGTGA
     1501 GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG
     1561 TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGTGAGGG CGATGCCACC TACGGCAAGC
     1621 TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA
     1681 CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG
     1741 ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACATCCAGGA GCGCACCATC TTCTTCAAGG
     1801 ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC
     1861 GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG
     1921 AGTACAACCT GCCGGACCAA CTGACTGAAG AGCAGATCGC AGAATTTAAA GAGGAATTCT
     1981 CCCTATTTGA CAAGGACGGG GATGGGACAA TAACAACCAA GGAGCTGGGG ACGGTGATGC
     2041 GGTCTCTGGG GCAGAACCCC ACAGAAGCAG AGCTGCAGGA CATGATCAAT GAAGTAGATG
     2101 CCGACGGTGA CGGCACAATC GACTTCCCTG AGTTCCTGAC AATGATGGCA AGAAAAATGA
     2161 AATACAGGGA CACGGAAGAA GAAATTAGAG AAGCGTTCGG TGTGTTTGAT AAGGATGGCA
     2221 ATGGCTACAT CAGTGCAGCA GAGCTTCGCC ACGTGATGAC AAACCTTGGA GAGAAGTTAA
     2281 CAGATGAAGA GGTTGATGAA ATGATCAGGG AAGCAGACAT CGATGGGGAT GGTCAGGTAA
     2341 ACTACGAAGA GTTTGTACAA ATGATGACAG CGAAGTGAgg taccctcgag tctggtaaag
     2401 aaaccgctgc tgcgaaattt gaacgccagc acatggactc gtctactagc gcagcttaat
     2461 taacctaggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
     2521 gggtcttgag gggttttttg ctgaaaggag gaactatatc cggattggcg aatgggacgc
     2581 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     2641 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     2701 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     2761 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     2821 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     2881 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     2941 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     3001 gaattttaac aaaatattaa cgtttacaat ttctggcggc acgatggcat gagattatca
     3061 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     3121 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     3181 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     3241 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     3301 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     3361 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     3421 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     3481 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     3541 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     3601 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     3661 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     3721 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     3781 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     3841 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     3901 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     3961 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     4021 caatcatgat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     4081 tatttagaaa aataaacaaa taggtcatga ccaaaatccc ttaacgtgag ttttcgttcc
     4141 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     4201 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     4261 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     4321 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     4381 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     4441 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     4501 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     4561 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     4621 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     4681 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     4741 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     4801 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     4861 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     4921 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
     4981 atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc
     5041 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
     5101 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     5161 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     5221 ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag
     5281 atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg
     5341 cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc
     5401 gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc
     5461 acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa
     5521 ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc
     5581 gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg
     5641 aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg
     5701 aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt
     5761 cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg
     5821 gtcctcaacg acaggagcac gatcatgcta gtcatgcccc gcgcccaccg gaaggagctg
     5881 actgggttga aggctctcaa gggcatcggt cgagatcccg gtgcctaatg agtgagctaa
     5941 cttacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     6001 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt
     6061 ggtttttctt ttcaccagtg agacgggcaa cagctgattg cccttcaccg cctggccctg
     6121 agagagttgc agcaagcggt ccacgctggt ttgccccagc aggcgaaaat cctgtttgat
     6181 ggtggttaac ggcgggatat aacatgagct gtcttcggta tcgtcgtatc ccactaccga
     6241 gatgtccgca ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc ccagcgccat
     6301 ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc tcattcagca tttgcatggt
     6361 ttgttgaaaa ccggacatgg cactccagtc gccttcccgt tccgctatcg gctgaatttg
     6421 attgcgagtg agatatttat gccagccagc cagacgcaga cgcgccgaga cagaacttaa
     6481 tgggcccgct aacagcgcga tttgctggtg acccaatgcg accagatgct ccacgcccag
     6541 tcgcgtaccg tcttcatggg agaaaataat actgttgatg ggtgtctggt cagagacatc
     6601 aagaaataac gccggaacat tagtgcaggc agcttccaca gcaatggcat cctggtcatc
     6661 cagcggatag ttaatgatca gcccactgac gcgttgcgcg agaagattgt gcaccgccgc
     6721 tttacaggct tcgacgccgc ttcgttctac catcgacacc accacgctgg cacccagttg
     6781 atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga
     6841 ggtggcaacg ccaatcagca acgactgttt gcccgccagt tgttgtgcca cgcggttggg
     6901 aatgtaattc agctccgcca tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg
     6961 gctggcctgg ttcaccacgc gggaaacggt ctgataagag acaccggcat actctgcgac
     7021 atcgtataac gttactggtt tcacattcac caccctgaat tgactctctt ccgggcgcta
     7081 tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg tccgggatct cgacgctctc
     7141 ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg
     7201 ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc
     7261 ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt
     7321 ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc
     7381 cggccacgat gcgtccggcg tagaggatcg agatcgatct cgatcccgcg aaattaatac
     7441 gactcactat a    
//
