LOCUS       pAAV.CAG.GFPsm_myc.WPRE.SV40(p3511)\Q        6108 bp    DNA     circular     17-SEP-2014
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|709286786|
COMMENT     VNTDBDATE|709296830|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAV.CAG.GFPsm_myc.WPRE.SV40(p3511) Q|
COMMENT     VNTAUTHORNAME|Shu Jen chen|
COMMENT     VNTOAUTHORNAME|Audrey Mazza|
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FEATURES             Location/Qualifiers
     repeat_region   1..168
                     /vntifkey="34"
                     /label=ITR
     repeat_region   complement(3120..3287)
                     /vntifkey="34"
                     /label=ITR
     promoter        193..1126
                     /vntifkey="29"
                     /label=CBA\promoter
     misc_marker     2244..2832
                     /vntifkey="22"
                     /label=WPRE
     polyA_signal    2854..3092
                     /vntifkey="25"
                     /label=sv40
     CDS             1142..2227
                     /vntifkey="4"
                     /label=GFPsm
BASE COUNT     1393 a      1574 c      1652 g      1489 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct 
      181 aggaagagta ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac 
      241 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca 
      301 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg 
      361 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt 
      421 agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc 
      481 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat 
      541 gggggcgggg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg 
      601 cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc 
      661 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg 
      721 agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc 
      781 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc 
      841 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag 
      901 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggc tgtccgcggg 
      961 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg 
     1021 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg 
     1081 gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattggat cggccgccac 
     1141 catggagcaa aaactgatat cggaggaaga cttggcagag cagaaactga tttcggaaga 
     1201 ggatcttgcc gaacaaaaac tgatcagtga ggaagacctt gccggagtgt cgaagggtga 
     1261 agagctgttc accggcgtgg tgcccatcct cgtcgagctg gacggagatg tcaatggaca 
     1321 taaattctcc gtacggggag agggcgaggg cgacgcaacc aacggtaaac tgaccctgaa 
     1381 gttcatttgc accacgggta aactgcccgt accctggccc accctggtca caaccttggg 
     1441 tggcggagtt cagtgttttt cgcggtatcc agatcacatg aagcagcatg actttttcaa 
     1501 gagcgctatg cccgagggtt acgttcaaga gaggaccatc agtttcaagg acgacggcac 
     1561 ctacaagaca cgggctgagg tgaaattcga gggtgataca ctcgtgaatc gtatcgagct 
     1621 gaagggcata gattttaagg aggatggcaa tatcctgggc cacaagttgg agtataactt 
     1681 caacagtcac aatgtctata taactgccga taagcagaag aatggcatta aagccaattt 
     1741 caaaattcgt cacaatgtcg aaggcggcgc agaacagaag ctcatcagtg aagaggatct 
     1801 ggccgcagag cagaaactca ttagcgagga ggacttgggc ggaggcggcg agcagaagct 
     1861 gatatcggag gaagacctgg cggccgagca aaagctcatc tcggaggagg atctcgcggg 
     1921 aggagatgga agtgtccagt tggcggatca ctaccagcag aatacgccaa ttggtgacgg 
     1981 ccccgtcctt ctgccggata accactacct ctccacccag tcggtgctga gcaaggaccc 
     2041 gaatgaaaaa cgtgaccaca tggttcttct tgagttcgtc acggctgcgg gcatcacgtt 
     2101 gggcatggac gaactgtaca agggcgctga acagaaactt atctccgagg aggatctggc 
     2161 ggaacagaag ctgatcagcg aagaggacct ggcggagcag aaacttatca gcgaagagga 
     2221 tctgtaaacc ggagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga 
     2281 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 
     2341 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 
     2401 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 
     2461 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 
     2521 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 
     2581 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat 
     2641 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 
     2701 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 
     2761 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg 
     2821 ccgcctcccc gcatcgatac cgtcgacccg ggcggccgct tcgagcagac atgataagat 
     2881 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg 
     2941 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca 
     3001 acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag gttttttaaa 
     3061 gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tcttcctaga gcatggctac 
     3121 gtagataagt agcatggcgg gttaatcatt aactacaagg aacccctagt gatggagttg 
     3181 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga 
     3241 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagcct taattaacct 
     3301 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt 
     3361 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc 
     3421 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc 
     3481 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc 
     3541 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc 
     3601 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc 
     3661 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg 
     3721 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact 
     3781 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt 
     3841 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa 
     3901 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt 
     3961 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 
     4021 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta 
     4081 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag 
     4141 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca 
     4201 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta 
     4261 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc 
     4321 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc 
     4381 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca 
     4441 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc 
     4501 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca 
     4561 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac 
     4621 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg 
     4681 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg 
     4741 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg 
     4801 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac 
     4861 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc 
     4921 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct 
     4981 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc 
     5041 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 
     5101 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 
     5161 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 
     5221 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 
     5281 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 
     5341 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 
     5401 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 
     5461 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 
     5521 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 
     5581 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 
     5641 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 
     5701 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg 
     5761 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc 
     5821 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg 
     5881 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca 
     5941 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact 
     6001 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa 
     6061 acagctatga ccatgattac gccagattta attaaggcct taattagg 
//
