LOCUS       pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40\(p2724)\Q        6984 bp    DNA     circular     12-NOV-2012
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|642087538|
COMMENT     VNTDBDATE|642513864|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40 (p2724) Q|
COMMENT     VNTAUTHORNAME|Shu-Jen Chen|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
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FEATURES             Location/Qualifiers
     promoter        233..720
                     /vntifkey="29"
                     /label=Synapsin\promoter
     intron          721..860
                     /vntifkey="15"
                     /label=Intron
     misc_marker     3060..3700
                     /vntifkey="22"
                     /label=wpre
     misc_feature    868..901
                     /vntifkey="21"
                     /label=Misc_Feature_5
                     /note="loxp2272"
     misc_feature    964..997
                     /vntifkey="21"
                     /label=loxp
     misc_feature    complement(2876..2909)
                     /vntifkey="21"
                     /label=loxp2272
     misc_feature    complement(2980..3013)
                     /vntifkey="21"
                     /label=loxp
     repeat_region   1..168
                     /vntifkey="34"
                     /label=5'\ITR
                     /note="ITR"
     repeat_region   complement(3996..4163)
                     /vntifkey="34"
                     /label=3'\ITR
     misc_feature    197..215
                     /vntifkey="21"
                     /label=AA0208\\SacI\480\f
     misc_feature    complement(3055..3072)
                     /vntifkey="21"
                     /label=AA0208\HindIII\1483r
     misc_feature    complement(3259..3278)
                     /vntifkey="21"
                     /label=AAV1621r
     misc_feature    complement(841..860)
                     /vntifkey="21"
                     /label=Misc_Feature_14
                     /note="intron AscI 3'r"
     misc_feature    233..253
                     /vntifkey="21"
                     /label=Syn\speI\5'f
     misc_feature    6726..6726
                     /vntifkey="21"
                     /label=seq\data\1108221\AAVsyn_FLEX_prep0819.WPRE_200Rev
     misc_feature    962..962
                     /vntifkey="21"
                     /label=C\to\A\switch\indicated\in\sequencing
     misc_feature    251..269
                     /vntifkey="21"
                     /label=PCR_primer\\syn\forward\primer\cactggacaagcacccaac
     misc_feature    complement(3156..3175)
                     /vntifkey="21"
                     /label=PCR_primer\\WPRE\rev\WPRE\rev\ttaaagcagcgtatccacat
                     /note="Unknown feature type:"
     misc_feature    complement(1295..1735)
                     /vntifkey="21"
                     /label=cpGFP\1-146
     misc_feature    complement(1022..1171)
                     /ApEinfo_fwdcolor=#a58d2a
                     /ApEinfo_revcolor=#a58d2a
                     /vntifkey="21"
                     /label=PDGFR\513-561
     misc_feature    complement(1175..1204)
                     /ApEinfo_fwdcolor=#fe84ff
                     /ApEinfo_revcolor=green
                     /vntifkey="21"
                     /label=myc_epi
     misc_feature    complement(1238..1294)
                     /vntifkey="21"
                     /label=YbeJ\254-272
     misc_feature    complement(1217..1237)
                     /vntifkey="21"
                     /label=YbeJ\%C6272-280
     misc_feature    complement(1754..2029)
                     /vntifkey="21"
                     /label=cpGFP\147-238
     misc_feature    complement(1736..1753)
                     /vntifkey="21"
                     /label=Linker
     misc_feature    complement(2030..2776)
                     /vntifkey="21"
                     /label=YbeJ5-253
     misc_feature    complement(2777..2782)
                     /vntifkey="21"
                     /label=MCS
     misc_feature    1995..1995
                     /vntifkey="21"
                     /label=MISMATCH:\T>C\(Lys-->Arg)
     misc_feature    1634..1634
                     /vntifkey="21"
                     /label=MISMATCH:\G>A\(Silent)
     CDS             complement(1025..2845)
                     /vntifkey="4"
                     /label=iGluSnFr
     misc_feature    868..901
                     /vntifkey="21"
                     /label=loxP2272
BASE COUNT     1540 a      1874 c      1803 g      1767 t 
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct 
      181 aggaagatcc gagctccagt gtgctggaat tcgccctttt cagtatttaa attgacgacc 
      241 gaccccgacc cactggacaa gcacccaacc cccattcccc aaattgcgca tcccctatca 
      301 gagaggggga ggggaaacag gatgcggcga ggcgcgtgcg cactgccagc ttcagcaccg 
      361 cggacagtgc cttcgccccc gcctggcggc gcgcgccacc gccgcctcag cactgaaggc 
      421 gcgctgacgt cactcgccgg tcccccgcaa actccccttc ccggccacct tggtcgcgtc 
      481 cgcgccgccg ccggcccagc cggaccgcac cacgcgaggc gcgagatagg ggggcacggg 
      541 cgcgaccatc tgcgctgcgg cgccggcgac tcagcgctgc ctcagtctgc ggtgggcagc 
      601 ggaggagtcg tgtcgtgcct gagagcgcag ctgtgctcct gggcaccgcg cagtccgccc 
      661 ccgcggctcc tggccagacc acccctagga ccccctgccc caagtcgcag ccgttggatc 
      721 aggtaagtat caaggttaca agacaggttt aaggagacca atagaaactg ggcttgtcga 
      781 gacagagaag actcttgcgt ttctgatagg cacctattgg tcttactgac atccactttg 
      841 cctttctctc cacaggtgat gatccccata acttcgtata aagtatccta tacgaagtta 
      901 tatcaaaata ggaagaccaa tgcttcacca tcgacccgaa ttgccaagca tcaccatcga 
      961 cacataactt cgtataatgt atgctatacg aagttatact agtgctagcg gatccgatat 
     1021 cctaacgtgg cttcttctgc caaagcatga tgaggatgat aagggagatg atggtgagca 
     1081 ccaccagggc caggatggct gagatcacca ccaccttaaa gggcaaggag tgtggcacca 
     1141 cgatgacctc ctgcgtgtcc tggcccacag cattcagatc ctcttctgag atgagttttt 
     1201 gttcgtcgac ctgcagtttc agtgccttgt cattcggttc tttgaacagt gctttcattt 
     1261 cgtctgacag ttcgaaattc atgttcaggg ggttgttaaa gttgtactcc agcttatgcc 
     1321 ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg 
     1381 tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga 
     1441 tggtgcgctc ctggatgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca 
     1501 tgtggtcggg gtagcggctg aagcactgca cgccgtaggt cagggtggtc acgagggtgg 
     1561 gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg 
     1621 tggcatcgcc ctcaccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca 
     1681 gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc accatgctcc 
     1741 ctccggtacc gcccttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga 
     1801 actccagcag gaccatgtga tcgcgcttct cgttggggtc tttcgaaagt ttggactggg 
     1861 tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct 
     1921 ggtagtggta ggcgagctgc acgccgccgt cctcgatgtt gtggcggatc ttgaagttcg 
     1981 ccttgatgcc gttcctctgc ttgtcggcca tgatatagac gttgtggctc accagaattg 
     2041 gatttttgaa ccacttatca aaccattttt ccgcttcacc ggaggtctgc acctgagcga 
     2101 tggtgtcatc catcagcttt ttgaactgcg gatcatcttt acgcaacata caaccgtagg 
     2161 cctcctgaga ctgcggcttg ccgacgattt cccagttgtc tggtttcttc gctttcgcac 
     2221 gttcaccggc cagcagcacg tcatccatca taaaggcaac ggcacgaccg ctttccaggg 
     2281 tgcggaaaga gtcaccgtga tctttggcgc tgatgatgcg catattcatt ttttgctctt 
     2341 cattcagttt gttgagcaaa acttcagagg tagtgccgga agtgacgact acggctttgt 
     2401 ctttcaggtt ggcaaaatct ttgatatcgc cacccttttt ggtcaacagg cgcgtaccga 
     2461 ccacgaaaat agtgtcagag aaagccgcct gtttttggcg ttcgacgttg ttggtggtag 
     2521 aaccacattc aaaatcgaaa gtgccgtttt gcagcagtgg aatacggttt tgtgaggtaa 
     2581 tcggaatcag ttttacctgc aagtccggtt tgttgagttt ctttttcact gcttcaacaa 
     2641 tggcgttgga gtaatcctgc gagtaaccca ccactttttg ctgattgtcg taataagaga 
     2701 aaggcactga agattcacgg tgaccgacga caatcacacc gtttttggcg attttgtcca 
     2761 gcgtgctgcc tgcggcagat ctgtcaccag tggaacctgg aacccagagc agcagtaccc 
     2821 atagcaggag tgtgtctgtc tccatggtgg cgatatcacc ggtggtacca ctagtataac 
     2881 ttcgtatagg atactttata cgaagttatc attgggattc ttcctatttt gatccaagca 
     2941 tcaccatcga ccctctagtc cagatctcac catcgaccca taacttcgta tagcatacat 
     3001 tatacgaagt tatgtccctc gaagaggttc gaaacactga aaagggcgaa ttctgcagat 
     3061 gatccactcg agaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg 
     3121 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct 
     3181 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg 
     3241 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact 
     3301 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc 
     3361 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc 
     3421 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa 
     3481 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc 
     3541 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg 
     3601 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg 
     3661 gccgcctccc cgcatcgata ccgtcgaccc gggcggccgc tcgagcatgc atctagagtc 
     3721 gacccgggcg gccgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca 
     3781 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat 
     3841 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt 
     3901 ttcaggttca gggggagatg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg 
     3961 gtaaaatcga taaggatctt cctagagcat ggctacgtag ataagtagca tggcgggtta 
     4021 atcattaact acaaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc 
     4081 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc 
     4141 tcagtgagcg agcgagcgcg cagccttaat taacctaatt cactggccgt cgttttacaa 
     4201 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct 
     4261 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc 
     4321 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg 
     4381 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc 
     4441 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc 
     4501 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt 
     4561 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag 
     4621 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg 
     4681 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag 
     4741 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg 
     4801 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 
     4861 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 
     4921 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 
     4981 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 
     5041 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 
     5101 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 
     5161 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 
     5221 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 
     5281 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 
     5341 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 
     5401 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 
     5461 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 
     5521 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 
     5581 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 
     5641 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 
     5701 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 
     5761 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 
     5821 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 
     5881 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 
     5941 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 
     6001 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 
     6061 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt 
     6121 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 
     6181 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 
     6241 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 
     6301 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 
     6361 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 
     6421 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 
     6481 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 
     6541 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 
     6601 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 
     6661 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 
     6721 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca 
     6781 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga 
     6841 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt 
     6901 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca 
     6961 gatttaatta aggccttaat tagg 
//
