LOCUS       Exported                6357 bp ds-DNA     circular SYN 19-JUN-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA-NFLAG-SF2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6357)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 19, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
COMMENT     LOCUS pcDNA3.1/Hygro(- 7716 bp DNA circular SYN 28-MAR-2011 SOURCE 
            ORGANISM COMMENT This file is created by Vector NTI 
            http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT 
            VNTDATE|585850619| COMMENT VNTDBDATE|585859653| COMMENT LSOWNER| 
            COMMENT VNTNAME|pcDNA3.1/Hygro(-)-N-Flag-del-EcoRI-NF90| COMMENT 
            VNTAUTHORNAME|Demo User| COMMENT VNTOAUTHORNAME|SONG WENJUN|
FEATURES             Location/Qualifiers
     source          1..6357
                     /organism="synthetic DNA construct"
                     /label=147
                     /note="color: #000000"
     promoter        232..819
                     /label=CMV promoter
                     /note="color: #ff8080"
     primer_bind     769..789
                     /label=CMV forward primer
                     /note="color: #80ff80; direction: RIGHT"
     primer_bind     863..882
                     /label=T7 primer
                     /note="color: #80ff80; direction: RIGHT"
     promoter        863..879
                     /label=T7 promoter
                     /note="color: #ff8080"
     CDS             909..935
                     /label=FLAG
                     /note="color: #ff0000; direction: RIGHT"
     CDS             987..1733
                     /codon_start=1
                     /note="SF2 iso1"
                     /note="color: #0080ff"
                     /translation="MSGGGVIRGPAGNNDCRIYVGNLPPDIRTKDIEDVFYKYGAIRDI
                     DLKNRRGGPPFAFVEFEDPRDAEDAVYGRDGYDYDGYRLRVEFPRSGRGTGRGGGGGGG
                     GGAPRGRYGPPSRRSENRVVVSGLPPSGSWQDLKDHMREAGDVCYADVYRDGTGVVEFV
                     RKEDMTYAVRKLDNTKFRSHEGETAYIRVKVDGPRSPSYGRSRSRSRSRSRSRSRSNSR
                     SRSYSPRRSRGSPRYSPRHSRSRSRT"
     misc_feature    987..1577
                     /note="RBM"
                     /note="color: #0080ff"
     misc_feature    1482..1484
                     /note="K166E"
                     /note="color: #0080ff"
     misc_feature    1578..1730
                     /note="RS domain"
                     /note="color: #0080ff"
     primer_bind     1778..1795
                     /label=BGH reverse primer
                     /note="color: #80ff80; direction: RIGHT"
     polyA_site      1784..2008
                     /label=BGH pA
                     /note="color: #ff0080"
     rep_origin      2054..2482
                     /direction=RIGHT
                     /label=f1 origin
                     /note="color: #c0c0c0"
     promoter        2487..2857
                     /label=SV40 early promoter
                     /note="color: #ff8080"
     CDS             2875..3900
                     /label=Hygro(R)
                     /note="color: #ff0000; direction: RIGHT"
     misc_feature    3118..3123
                     /label=delEcoRI
                     /note="color: #0080ff"
     polyA_signal    4030..4160
                     /label=SV40 pA
                     /note="color: #ff0080"
     rep_origin      complement(4543..5216)
                     /direction=LEFT
                     /label=pUC origin
                     /note="color: #c0c0c0"
     CDS             complement(5361..6221)
                     /label=Amp(R)
                     /note="color: #ff0000; direction: LEFT"
     promoter        complement(6222..6320)
                     /label=bla promoter
                     /note="color: #ff8080; direction: LEFT"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaacat ggattacaag gatgacgacg ataaggggcc ctctagactc gagcggccgc
      961 cactgtgctg gatatctgca gaattcatgt cgggaggtgg tgtgattcgt ggccccgcag
     1021 ggaacaacga ttgccgcatc tacgtgggta acttacctcc agacatccga accaaggaca
     1081 ttgaggacgt gttctacaaa tacggcgcta tccgcgacat cgacctcaag aatcgccgcg
     1141 ggggaccgcc cttcgccttc gttgagttcg aggacccgcg agacgcggaa gacgcggtgt
     1201 atggtcgcga cggctatgat tacgatgggt accgtctgcg ggtggagttt cctcgaagcg
     1261 gccgtggaac aggccgaggc ggcggcgggg gtggaggtgg cggagctccc cgaggtcgct
     1321 atggcccccc atccaggcgg tctgaaaaca gagtggttgt ctctggactg cctccaagtg
     1381 gaagttggca ggatttaaag gatcacatgc gtgaagcagg tgatgtatgt tatgctgatg
     1441 tttaccgaga tggcactggt gtcgtggagt ttgtacggaa agaagatatg acctatgcag
     1501 ttcgaaaact ggataacact aagtttagat ctcatgaggg agaaactgcc tacatccggg
     1561 ttaaagttga tgggcccaga agtccaagtt atggaagatc tcgatctcga agccgtagtc
     1621 gtagcagaag ccgtagcaga agcaacagca ggagtcgcag ttactcccca aggagaagca
     1681 gaggatcacc acgctattct ccccgtcata gcagatctcg ctctcgtaca taaggatccg
     1741 agctcggtac caagcttaag tttaaaccgc tgatcagcct cgactgtgcc ttctagttgc
     1801 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     1861 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     1921 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     1981 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     2041 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     2101 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     2161 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg catcccttta
     2221 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     2281 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     2341 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     2401 tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa tgagctgatt
     2461 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     2521 ccccaggctc cccaggcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     2581 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     2641 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     2701 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     2761 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     2821 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgatgaaa
     2881 aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc
     2941 tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga
     3001 gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat
     3061 gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggga
     3121 ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac
     3181 ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc
     3241 gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt
     3301 caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg
     3361 caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg
     3421 ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac
     3481 aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc
     3541 ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg
     3601 gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc
     3661 cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat
     3721 ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg
     3781 actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta
     3841 gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaatag
     3901 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     3961 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     4021 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     4081 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     4141 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     4201 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     4261 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     4321 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     4381 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     4441 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     4501 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     4561 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     4621 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     4681 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     4741 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc
     4801 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     4861 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     4921 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     4981 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     5041 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     5101 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     5161 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     5221 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     5281 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     5341 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     5401 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     5461 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     5521 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     5581 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     5641 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     5701 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     5761 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     5821 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     5881 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     5941 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     6001 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     6061 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     6121 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     6181 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     6241 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     6301 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
