LOCUS Exported 5197 bp ds-DNA circular SYN 21-JUN-2017 DEFINITION Tet-inducible mTurquoise2. ACCESSION . VERSION . KEYWORDS pAAV-TRE-mTurquoise2 SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 5197) AUTHORS Ben TITLE Direct Submission JOURNAL Exported Thursday, Jun 22, 2017 from SnapGene 3.1.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5197 /organism="recombinant plasmid" /mol_type="other DNA" misc_feature 1..141 /note="L-ITR" misc_feature 176..194 /note="TetO" misc_feature 213..231 /note="TetO" misc_feature 250..268 /note="TetO" misc_feature 287..305 /note="TetO" misc_feature 324..342 /note="TetO" misc_feature 361..379 /note="TetO" misc_feature 398..416 /note="TetO" misc_feature 435..453 /note="TetO" /note="Tet binding" CDS 466..561 /codon_start=1 /note="CMV2_promoter" /translation="YKQSSFSEPSDRLETPSTLF*PP*KTPGPIQP" CDS 580..1299 /codon_start=1 /note="mTurquoise2" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" misc_feature 1314..1922 /note="WPRE" polyA_signal 1942..2420 /note="hGH polyA" misc_feature 2460..2600 /note="R-ITR" rep_origin 2692..2998 /direction=RIGHT /note="f1 Origin" misc_feature 3517..4374 /note="AmpR" rep_origin 4525..5192 /direction=RIGHT /note="pUC ori" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtatcaa gtgccacctg acgtctccct 181 atcagtgata gagaagtcga cacgtctcga gctccctatc agtgatagag aaggtacgtc 241 tagaacgtct ccctatcagt gatagagaag tcgacacgtc tcgagctccc tatcagtgat 301 agagaaggta cgtctagaac gtctccctat cagtgataga gaagtcgaca cgtctcgagc 361 tccctatcag tgatagagaa ggtacgtcta gaacgtctcc ctatcagtga tagagaagtc 421 gacacgtctc gagctcccta tcagtgatag agaagctacc ccctatataa gcagagctcg 481 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag 541 acaccgggac cgatccagcc tggatccggt accgccacca tggtgagcaa gggcgaggag 601 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag 661 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc 721 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgtcctgg 781 ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc 841 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac 901 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag 961 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacttc 1021 agcgacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag 1081 atccgccaca acatcgagga cggcggcgtg cagctcgccg accactacca gcagaacacc 1141 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag 1201 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc 1261 gccgggatca ctctcggcat ggacgagctg tacaagtgag aattcgatat caagcttatc 1321 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt 1381 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc 1441 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag 1501 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc 1561 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc 1621 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg 1681 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg 1741 ctcgcctatg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc 1801 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt 1861 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac 1921 cgagcgctgc tcgagagatc tacgggtggc atccctgtga cccctcccca gtgcctctcc 1981 tggccctgga agttgccact ccagtgccca ccagccttgt cctaataaaa ttaagttgca 2041 tcattttgtc tgactaggtg tccttctata atattatggg gtggaggggg gtggtatgga 2101 gcaaggggca agttgggaag acaacctgta gggcctgcgg ggtctattgg gaaccaagct 2161 ggagtgcagt ggcacaatct tggctcactg caatctccgc ctcctgggtt caagcgattc 2221 tcctgcctca gcctcccgag ttgttgggat tccaggcatg catgaccagg ctcagctaat 2281 ttttgttttt ttggtagaga cggggtttca ccatattggc caggctggtc tccaactcct 2341 aatctcaggt gatctaccca ccttggcctc ccaaattgct gggattacag gcgtgaacca 2401 ctgctccctt ccctgtcctt ctgattttgt aggtaaccac gtgcggaccg agcggccgca 2461 ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc 2521 cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg 2581 agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg 2641 tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc 2701 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc 2761 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct 2821 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa 2881 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc 2941 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca 3001 ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat 3061 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg 3121 tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag 3181 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc 3241 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca 3301 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc 3361 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc 3421 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc 3481 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc 3541 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg 3601 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat 3661 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc 3721 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa 3781 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa 3841 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt 3901 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct 3961 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat 4021 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg 4081 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg 4141 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt 4201 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg 4261 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg 4321 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg 4381 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa 4441 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt 4501 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 4561 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 4621 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 4681 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta 4741 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 4801 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 4861 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 4921 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 4981 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 5041 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 5101 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 5161 cggttcctgg ccttttgctg gccttttgct cacatgt //