LOCUS       Exported                5197 bp ds-DNA     circular SYN 21-JUN-2017
DEFINITION  Tet-inducible mTurquoise2.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-TRE-mTurquoise2
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Ben
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jun 22, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    1..141
                     /note="L-ITR"
     misc_feature    176..194
                     /note="TetO"
     misc_feature    213..231
                     /note="TetO"
     misc_feature    250..268
                     /note="TetO"
     misc_feature    287..305
                     /note="TetO"
     misc_feature    324..342
                     /note="TetO"
     misc_feature    361..379
                     /note="TetO"
     misc_feature    398..416
                     /note="TetO"
     misc_feature    435..453
                     /note="TetO"
                     /note="Tet binding"
     CDS             466..561
                     /codon_start=1
                     /note="CMV2_promoter"
                     /translation="YKQSSFSEPSDRLETPSTLF*PP*KTPGPIQP"
     CDS             580..1299
                     /codon_start=1
                     /note="mTurquoise2"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    1314..1922
                     /note="WPRE"
     polyA_signal    1942..2420
                     /note="hGH polyA"
     misc_feature    2460..2600
                     /note="R-ITR"
     rep_origin      2692..2998
                     /direction=RIGHT
                     /note="f1 Origin"
     misc_feature    3517..4374
                     /note="AmpR"
     rep_origin      4525..5192
                     /direction=RIGHT
                     /note="pUC ori"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtatcaa gtgccacctg acgtctccct
      181 atcagtgata gagaagtcga cacgtctcga gctccctatc agtgatagag aaggtacgtc
      241 tagaacgtct ccctatcagt gatagagaag tcgacacgtc tcgagctccc tatcagtgat
      301 agagaaggta cgtctagaac gtctccctat cagtgataga gaagtcgaca cgtctcgagc
      361 tccctatcag tgatagagaa ggtacgtcta gaacgtctcc ctatcagtga tagagaagtc
      421 gacacgtctc gagctcccta tcagtgatag agaagctacc ccctatataa gcagagctcg
      481 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
      541 acaccgggac cgatccagcc tggatccggt accgccacca tggtgagcaa gggcgaggag
      601 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
      661 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
      721 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgtcctgg
      781 ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
      841 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
      901 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
      961 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacttc
     1021 agcgacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag
     1081 atccgccaca acatcgagga cggcggcgtg cagctcgccg accactacca gcagaacacc
     1141 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag
     1201 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     1261 gccgggatca ctctcggcat ggacgagctg tacaagtgag aattcgatat caagcttatc
     1321 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     1381 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     1441 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     1501 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     1561 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     1621 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     1681 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     1741 ctcgcctatg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     1801 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     1861 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac
     1921 cgagcgctgc tcgagagatc tacgggtggc atccctgtga cccctcccca gtgcctctcc
     1981 tggccctgga agttgccact ccagtgccca ccagccttgt cctaataaaa ttaagttgca
     2041 tcattttgtc tgactaggtg tccttctata atattatggg gtggaggggg gtggtatgga
     2101 gcaaggggca agttgggaag acaacctgta gggcctgcgg ggtctattgg gaaccaagct
     2161 ggagtgcagt ggcacaatct tggctcactg caatctccgc ctcctgggtt caagcgattc
     2221 tcctgcctca gcctcccgag ttgttgggat tccaggcatg catgaccagg ctcagctaat
     2281 ttttgttttt ttggtagaga cggggtttca ccatattggc caggctggtc tccaactcct
     2341 aatctcaggt gatctaccca ccttggcctc ccaaattgct gggattacag gcgtgaacca
     2401 ctgctccctt ccctgtcctt ctgattttgt aggtaaccac gtgcggaccg agcggccgca
     2461 ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc
     2521 cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg
     2581 agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     2641 tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
     2701 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     2761 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     2821 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     2881 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     2941 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     3001 ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     3061 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     3121 tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     3181 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     3241 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     3301 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     3361 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     3421 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     3481 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     3541 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     3601 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     3661 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     3721 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     3781 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     3841 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     3901 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     3961 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     4021 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     4081 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     4141 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     4201 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     4261 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     4321 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     4381 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     4441 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     4501 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     4561 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     4621 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     4681 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     4741 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     4801 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     4861 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     4921 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     4981 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     5041 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     5101 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     5161 cggttcctgg ccttttgctg gccttttgct cacatgt
//
