LOCUS       Exported                5471 bp ds-DNA     circular SYN 21-JUN-2017
DEFINITION  VAST construct: Tet-inducible, Cre-dependent mTurquoise2.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-TRE-DIO-mTurquoise2
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5471)
  AUTHORS   Ben
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jun 22, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5471
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    1..141
                     /note="L-ITR"
     misc_feature    176..194
                     /note="TetO"
     misc_feature    213..231
                     /note="TetO"
     misc_feature    250..268
                     /note="TetO"
     misc_feature    287..305
                     /note="TetO"
     misc_feature    324..342
                     /note="TetO"
     misc_feature    361..379
                     /note="TetO"
     misc_feature    398..416
                     /note="TetO"
     misc_feature    435..453
                     /note="TetO"
                     /note="Tet binding"
     promoter        466..561
                     /note="CMV2_promoter"
     misc_feature    598..631
                     /note="lox2722"
     misc_feature    682..715
                     /note="loxP"
     CDS             complement(724..1443)
                     /codon_start=1
                     /note="mTurquoise2"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    1289
                     /note="Untitled"
     misc_feature    complement(1456..1489)
                     /note="lox2722"
     misc_feature    complement(1540..1573)
                     /note="loxP"
     misc_feature    1588..2196
                     /note="WPRE"
     polyA_signal    2216..2694
                     /note="hGH polyA"
     misc_feature    2734..2874
                     /note="R-ITR"
     rep_origin      2966..3272
                     /direction=RIGHT
                     /note="f1 Origin"
     misc_feature    3791..4648
                     /note="AmpR"
     rep_origin      4799..5466
                     /direction=RIGHT
                     /note="pUC ori"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtatcaa gtgccacctg acgtctccct
      181 atcagtgata gagaagtcga cacgtctcga gctccctatc agtgatagag aaggtacgtc
      241 tagaacgtct ccctatcagt gatagagaag tcgacacgtc tcgagctccc tatcagtgat
      301 agagaaggta cgtctagaac gtctccctat cagtgataga gaagtcgaca cgtctcgagc
      361 tccctatcag tgatagagaa ggtacgtcta gaacgtctcc ctatcagtga tagagaagtc
      421 gacacgtctc gagctcccta tcagtgatag agaagctacc ccctatataa gcagagctcg
      481 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
      541 acaccgggac cgatccagcc tggatccggt accggatcct ctagagtcga ctccggaata
      601 acttcgtata ggatacttta tacgaagtta tgcagaatgg tagctggatt gtagctgcta
      661 ttagcaatat gaaacctctt aataacttcg tatagcatac attatacgaa gttatggcgc
      721 gcctcacttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag
      781 caggaccatg tgatcgcgct tctcgttggg gtctttgctc agcttggact gggtgctcag
      841 gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg
      901 gtcggcgagc tgcacgccgc cgtcctcgat gttgtggcgg atcttgaagt tggccttgat
      961 gccgttcttc tgcttgtcgg cggtgatata gacgttgtcg ctgaagtagt tgtactccag
     1021 cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt
     1081 caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt
     1141 gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg
     1201 ctgcttcatg tggtcggggt agcgggcgaa gcactgcacg ccccaggaca gggtggtcac
     1261 gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt
     1321 gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc
     1381 gccgtccagc tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcac
     1441 catggtggcg ctagcataac ttcgtataaa gtatcctata cgaagttatt tgccttaacc
     1501 cagaaattat cactgttatt ctttagaatg gtgcaaagaa taacttcgta taatgtatgc
     1561 tatacgaagt tatgaattcg atatcaagct tatcgataat caacctctgg attacaaaat
     1621 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     1681 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     1741 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     1801 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     1861 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     1921 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     1981 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tatgttgcca cctggattct
     2041 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     2101 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     2161 gatctccctt tgggccgcct ccccgcatcg ataccgagcg ctgctcgaga gatctacggg
     2221 tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg
     2281 cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc
     2341 tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc
     2401 tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc
     2461 actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg
     2521 ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt
     2581 ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg
     2641 cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt
     2701 ttgtaggtaa ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca
     2761 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc
     2821 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc
     2881 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca
     2941 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     3001 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     3061 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     3121 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg
     3181 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     3241 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt
     3301 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     3361 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag
     3421 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     3481 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     3541 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     3601 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     3661 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     3721 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     3781 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     3841 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     3901 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     3961 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     4021 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     4081 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     4141 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     4201 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     4261 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     4321 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     4381 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     4441 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     4501 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     4561 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     4621 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     4681 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     4741 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     4801 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     4861 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     4921 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     4981 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     5041 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     5101 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     5161 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     5221 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     5281 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     5341 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     5401 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     5461 tgctcacatg t
//
