LOCUS       Exported                6182 bp ds-DNA     circular SYN 21-JUN-2017
DEFINITION  VAST construct: Tet-inducible, Cre-dependent tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-TRE-DIO-tdTomato
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6182)
  AUTHORS   Ben
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jun 22, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6182
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    1..141
                     /note="L-ITR"
     misc_feature    176..194
                     /note="TetO"
     misc_feature    213..231
                     /note="TetO"
     misc_feature    250..268
                     /note="TetO"
     misc_feature    287..305
                     /note="TetO"
     misc_feature    324..342
                     /note="TetO"
     misc_feature    361..379
                     /note="TetO"
     misc_feature    398..416
                     /note="TetO"
     misc_feature    435..453
                     /note="TetO"
                     /note="Tet binding"
     promoter        466..561
                     /note="CMV2_promoter"
     misc_feature    598..631
                     /note="lox2722"
     misc_feature    682..715
                     /note="loxP"
     CDS             complement(724..2154)
                     /codon_start=1
                     /note="tdTomato"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGNYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    complement(2167..2200)
                     /note="lox2722"
     misc_feature    complement(2251..2284)
                     /note="loxP"
     misc_feature    2299..2907
                     /note="WPRE"
     polyA_signal    2927..3405
                     /note="hGH polyA"
     misc_feature    3445..3585
                     /note="R-ITR"
     rep_origin      3677..3983
                     /direction=RIGHT
                     /note="f1 Origin"
     misc_feature    4502..5359
                     /note="AmpR"
     rep_origin      5510..6177
                     /direction=RIGHT
                     /note="pUC ori"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtatcaa gtgccacctg acgtctccct
      181 atcagtgata gagaagtcga cacgtctcga gctccctatc agtgatagag aaggtacgtc
      241 tagaacgtct ccctatcagt gatagagaag tcgacacgtc tcgagctccc tatcagtgat
      301 agagaaggta cgtctagaac gtctccctat cagtgataga gaagtcgaca cgtctcgagc
      361 tccctatcag tgatagagaa ggtacgtcta gaacgtctcc ctatcagtga tagagaagtc
      421 gacacgtctc gagctcccta tcagtgatag agaagctacc ccctatataa gcagagctcg
      481 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
      541 acaccgggac cgatccagcc tggatccggt accggatcct ctagagtcga ctccggaata
      601 acttcgtata ggatacttta tacgaagtta tgcagaatgg tagctggatt gtagctgcta
      661 ttagcaatat gaaacctctt aataacttcg tatagcatac attatacgaa gttatggcgc
      721 gccttacttg tacagctcgt ccatgccgta caggaacagg tggtggcggc cctcggagcg
      781 ctcgtactgt tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca gcttggtgtc
      841 cacgtagtag tagccgggca gttgcacggg cttcttggcc atgtagatgg tcttgaactc
      901 caccaggtag ttgccgccgt ccttcagctt cagggcctgg tggatctcgc ccttcagcac
      961 gccgtcgcgg gggtacaggc gctcggtgga ggcctcccag cccatggtct tcttctgcat
     1021 tacggggccg tcggggggga agttggtgcc gcgcatcttc accttgtaga tcagcgtgcc
     1081 gtcctgcagg gaggagtcct gggtcacggt caccagaccg ccgtcctcga agttcatcac
     1141 gcgctcccac ttgaagccct cggggaagga cagcttcttg taatcgggga tgtcggcggg
     1201 gtgcttcacg tacgccttgg agccgtacat gaactggggg gacaggatgt cccaggcgaa
     1261 gggcaggggg ccgcccttgg tcaccttcag cttggcggtc tgggtgccct cgtaggggcg
     1321 gccctcgccc tcgccctcga tctcgaactc gtggccgttc atggagccct ccatgcgcac
     1381 cttgaagcgc atgaactctt tgatgacggc catgttgttg tcctcggagg aggcggtgcc
     1441 ggagctgccg ctgccggtgc tgccggtgcc atgccccagg aacaggtggt ggcggccctc
     1501 ggagcgctcg tactgttcca cgatggtgta gtcctcgttg tgggaggtga tgtccagctt
     1561 ggtgtccacg tagtagtagc cgggcagttg cacgggcttc ttggccatgt agatggtctt
     1621 gaactccacc aggtagtggc cgccgtcctt cagcttcagg gcctggtgga tctcgccctt
     1681 cagcacgccg tcgcgggggt acaggcgctc ggtggaggcc tcccagccca tggtcttctt
     1741 ctgcattacg gggccgtcgg gggggaagtt ggtgccgcgc atcttaacct tgtagatcag
     1801 cgtgccgtcc tgcagggagg agtcctgggt cacggtcacc agaccgccgt cctcgaagtt
     1861 catcacgcgc tcccacttga agccctcggg gaaggacagc ttcttgtaat cggggatgtc
     1921 ggcggggtgc ttcacgtacg ccttggagcc gtacatgaac tggggggaca ggatgtccca
     1981 ggcgaagggc agggggccgc ccttggtcac cttcagcttg gcggtctggg tgccctcgta
     2041 ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg ccgttcatgg agccctccat
     2101 gcgcaccttg aagcgcatga actctttgat gacctcctcg cccttgctca ccatggtggc
     2161 gctagcataa cttcgtataa agtatcctat acgaagttat ttgccttaac ccagaaatta
     2221 tcactgttat tctttagaat ggtgcaaaga ataacttcgt ataatgtatg ctatacgaag
     2281 ttatgaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
     2341 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     2401 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     2461 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     2521 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     2581 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     2641 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     2701 gaaatcatcg tcctttcctt ggctgctcgc ctatgttgcc acctggattc tgcgcgggac
     2761 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     2821 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     2881 ttgggccgcc tccccgcatc gataccgagc gctgctcgag agatctacgg gtggcatccc
     2941 tgtgacccct ccccagtgcc tctcctggcc ctggaagttg ccactccagt gcccaccagc
     3001 cttgtcctaa taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt
     3061 atggggtgga ggggggtggt atggagcaag gggcaagttg ggaagacaac ctgtagggcc
     3121 tgcggggtct attgggaacc aagctggagt gcagtggcac aatcttggct cactgcaatc
     3181 tccgcctcct gggttcaagc gattctcctg cctcagcctc ccgagttgtt gggattccag
     3241 gcatgcatga ccaggctcag ctaatttttg tttttttggt agagacgggg tttcaccata
     3301 ttggccaggc tggtctccaa ctcctaatct caggtgatct acccaccttg gcctcccaaa
     3361 ttgctgggat tacaggcgtg aaccactgct cccttccctg tccttctgat tttgtaggta
     3421 accacgtgcg gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc
     3481 tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg
     3541 cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt
     3601 attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta
     3661 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     3721 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     3781 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     3841 tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc
     3901 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     3961 actcttgttc caaactggaa caacactcaa ccctatctcg ggctattctt ttgatttata
     4021 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     4081 cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg
     4141 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     4201 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     4261 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     4321 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     4381 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     4441 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     4501 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     4561 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     4621 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     4681 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     4741 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     4801 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     4861 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     4921 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     4981 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     5041 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     5101 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     5161 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     5221 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     5281 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     5341 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     5401 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     5461 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     5521 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     5581 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     5641 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     5701 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     5761 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     5821 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     5881 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     5941 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6001 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6061 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6121 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6181 gt
//
