LOCUS       Exported                5465 bp ds-DNA     circular SYN 21-JUN-2017
DEFINITION  VAST construct: Tet-inducible, Cre-dependent mRuby2.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-TRE-DIO-mRuby2
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5465)
  AUTHORS   Ben
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jun 22, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5465
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    1..141
                     /note="L-ITR"
     misc_feature    176..194
                     /note="TetO"
     misc_feature    213..231
                     /note="TetO"
     misc_feature    250..268
                     /note="TetO"
     misc_feature    287..305
                     /note="TetO"
     misc_feature    324..342
                     /note="TetO"
     misc_feature    361..379
                     /note="TetO"
     misc_feature    398..416
                     /note="TetO"
     misc_feature    435..453
                     /note="TetO"
                     /note="Tet binding"
     CDS             466..561
                     /codon_start=1
                     /note="CMV2_promoter"
                     /translation="YKQSSFSEPSDRLETPSTLF*PP*KTPGPIQP"
     misc_feature    598..631
                     /note="lox2722"
     misc_feature    682..715
                     /note="loxP"
     misc_feature    complement(727..1437)
                     /note="mRuby2"
     misc_feature    complement(1450..1483)
                     /note="lox2722"
     misc_feature    complement(1534..1567)
                     /note="loxP"
     misc_feature    1582..2190
                     /note="WPRE"
     polyA_signal    2210..2688
                     /note="hGH polyA"
     misc_feature    2728..2868
                     /note="R-ITR"
     rep_origin      2960..3266
                     /direction=RIGHT
                     /note="f1 Origin"
     misc_feature    3785..4642
                     /note="AmpR"
     rep_origin      4793..5460
                     /direction=RIGHT
                     /note="pUC ori"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtatcaa gtgccacctg acgtctccct
      181 atcagtgata gagaagtcga cacgtctcga gctccctatc agtgatagag aaggtacgtc
      241 tagaacgtct ccctatcagt gatagagaag tcgacacgtc tcgagctccc tatcagtgat
      301 agagaaggta cgtctagaac gtctccctat cagtgataga gaagtcgaca cgtctcgagc
      361 tccctatcag tgatagagaa ggtacgtcta gaacgtctcc ctatcagtga tagagaagtc
      421 gacacgtctc gagctcccta tcagtgatag agaagctacc ccctatataa gcagagctcg
      481 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
      541 acaccgggac cgatccagcc tggatccggt accggatcct ctagagtcga ctccggaata
      601 acttcgtata ggatacttta tacgaagtta tgcagaatgg tagctggatt gtagctgcta
      661 ttagcaatat gaaacctctt aataacttcg tatagcatac attatacgaa gttatggcgc
      721 gcctcatttg tacagctcat ccattcctcc gccaaggcca gcaaacttgg caactgcatg
      781 ttcccgttgt accacaaaca tctcgttgtc agactcctcc agtcgttcca agcggtgatc
      841 aaccgcatgt atgccaggca ttttgatgtt gccgacagtt ttcttgctcc tgtacgtggt
      901 cacaaatgag catgacaagt ggcctcctcc gtcaaccttc agagccatgt gcgtatagcc
      961 tctcagtcct ccatctgcag gatacatcat ctctgtgttt ggctcccacc ctttggtttt
     1021 cttctgcatc acaggcccat tggagggaaa gttcacccct ctcacttgca cgtgatagac
     1081 cagacatccg tcctccagac tagtgtcctg catgactgtg accacaccac catcctcgta
     1141 acgggtcacc ctttcccatg tgaagccctc tggaaaggac tgtttgaaga agtctggaat
     1201 gcctttgggg tacttgatga aggtccgact gccatacatg aagctagtgg caagaatgtc
     1261 gaaggcaaac gggagagggc caccttcaat aaccttgatc ctcatggtct gagtacccat
     1321 gtagggattc ccttcacctt cccctgtgca cttgaactga tgcccattca cagacccttc
     1381 catgactacc ttcatgcgca tgttctcctt gatgagttcc tctcccttgc tcaccatggt
     1441 ggcgctagca taacttcgta taaagtatcc tatacgaagt tatttgcctt aacccagaaa
     1501 ttatcactgt tattctttag aatggtgcaa agaataactt cgtataatgt atgctatacg
     1561 aagttatgaa ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
     1621 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     1681 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     1741 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     1801 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     1861 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     1921 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     1981 ggggaaatca tcgtcctttc cttggctgct cgcctatgtt gccacctgga ttctgcgcgg
     2041 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     2101 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     2161 cctttgggcc gcctccccgc atcgataccg agcgctgctc gagagatcta cgggtggcat
     2221 ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc
     2281 agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat
     2341 attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg
     2401 gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca
     2461 atctccgcct cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc
     2521 caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc
     2581 atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc
     2641 aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct gattttgtag
     2701 gtaaccacgt gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct
     2761 ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct
     2821 ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc
     2881 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata
     2941 gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     3001 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     3061 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     3121 tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg
     3181 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     3241 tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt cttttgattt
     3301 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     3361 taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat
     3421 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     3481 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     3541 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     3601 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     3661 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     3721 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     3781 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     3841 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     3901 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     3961 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     4021 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     4081 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     4141 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     4201 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     4261 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     4321 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     4381 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     4441 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     4501 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     4561 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     4621 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     4681 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     4741 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     4801 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     4861 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     4921 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     4981 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     5041 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5101 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     5161 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     5221 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     5281 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     5341 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     5401 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     5461 catgt
//
