LOCUS       Exported                5929 bp ds-DNA     circular SYN 21-JUN-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-CAG-tTA
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5929)
  AUTHORS   Ken Chan
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jun 22, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5929
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    1..141
                     /note="L-ITR"
     misc_feature    151..1277
                     /note="KC103F"
     promoter        164..1263
                     /note="CAG"
     CDS             1285..2031
                     /codon_start=1
                     /note="tTA"
                     /translation="MSRLDKSKVINGALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    2046..2654
                     /note="WPRE"
     polyA_signal    2674..3152
                     /note="hGH polyA"
     misc_feature    3192..3332
                     /note="R-ITR"
     rep_origin      3424..3730
                     /direction=RIGHT
                     /note="f1 Origin"
     misc_feature    4249..5106
                     /note="AmpR"
     rep_origin      5257..5924
                     /direction=RIGHT
                     /note="pUC ori"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaacac tagttattaa tagtaatcaa
      181 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      301 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      481 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
      541 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
      601 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggcgc gcgccaggcg
      661 gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc
      721 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata
      781 aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct
      841 ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga
      901 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg
      961 tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagcgccgg caggaaggaa
     1021 atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct
     1081 cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc
     1141 ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt
     1201 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
     1261 ttgcggccca acggtaccgc caccatgagt cggctggata aatctaaagt cataaacggc
     1321 gctctggaat tactcaatga agtcggtatc gaaggcctga cgacaaggaa actcgctcaa
     1381 aagctgggag ttgagcagcc taccctgtac tggcacgtga agaacaagcg ggccctgctc
     1441 gatgccctgg ccatcgagat gctggacagg catcataccc acttctgccc cctggaaggc
     1501 gagtcatggc aagactttct gcggaacaac gccaagtcat tccgctgtgc tctcctctca
     1561 catcgcgacg gggctaaagt gcatctcggc acccgcccaa cagagaaaca gtacgaaacc
     1621 ctggaaaatc agctcgcgtt cctgtgtcag caaggcttct ccctggagaa cgcactgtac
     1681 gctctgtccg ccgtgggcca ctttacactg ggctgcgtat tggaggaaca ggagcatcaa
     1741 gtagcaaaag aggaaagaga gacacctacc accgattcta tgcccccact tctgagacaa
     1801 gcaattgagc tgttcgaccg gcagggagcc gaacctgcct tccttttcgg cctggaacta
     1861 atcatatgtg gcctggagaa acagctaaag tgcgaaagcg gcgggccggc cgacgccctt
     1921 gacgattttg acttagacat gctcccagcc gatgcccttg acgactttga ccttgatatg
     1981 ctgcctgctg acgctcttga cgattttgac cttgacatgc tccccgggta agaattcgat
     2041 atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     2101 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     2161 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     2221 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     2281 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     2341 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     2401 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     2461 tttccttggc tgctcgccta tgttgccacc tggattctgc gcgggacgtc cttctgctac
     2521 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     2581 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     2641 ccgcatcgat accgagcgct gctcgagaga tctacgggtg gcatccctgt gacccctccc
     2701 cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa
     2761 aattaagttg catcattttg tctgactagg tgtccttcta taatattatg gggtggaggg
     2821 gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc ggggtctatt
     2881 gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc gcctcctggg
     2941 ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca tgcatgacca
     3001 ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg gccaggctgg
     3061 tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg ctgggattac
     3121 aggcgtgaac cactgctccc ttccctgtcc ttctgatttt gtaggtaacc acgtgcggac
     3181 cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc
     3241 gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc
     3301 agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac
     3361 gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc
     3421 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     3481 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     3541 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     3601 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
     3661 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     3721 actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg
     3781 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     3841 aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca
     3901 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     3961 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     4021 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     4081 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     4141 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     4201 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     4261 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     4321 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     4381 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     4441 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     4501 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     4561 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     4621 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     4681 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     4741 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     4801 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     4861 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     4921 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     4981 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     5041 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     5101 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     5161 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     5221 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     5281 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     5341 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     5401 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     5461 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     5521 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     5581 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     5641 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     5701 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     5761 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     5821 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     5881 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt
//
