LOCUS       Exported                5466 bp ds-DNA     circular SYN 21-JUN-2017
DEFINITION  hSynapsin driven Tet Transactivator with positive feedback loop for 
            greater tTA expression at low vector doses.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-ihSyn1-tTA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jun 22, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..141
                     /note="L-ITR"
     exon            156..640
                     /note="Human Synapsin I promoter"
     misc_feature    657..774
                     /note="synthetic intron"
     misc_feature    673..691
                     /note="TetO"
     misc_feature    709..727
                     /note="TetO"
     CDS             822..1568
                     /codon_start=1
                     /note="tTA"
                     /translation="MSRLDKSKVINGALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    1583..2191
                     /note="WPRE"
     polyA_signal    2211..2689
                     /note="hGH polyA"
     misc_feature    2729..2869
                     /note="R-ITR"
     rep_origin      2961..3267
                     /direction=RIGHT
                     /note="f1 Origin"
     misc_feature    3786..4643
                     /note="AmpR"
     rep_origin      4794..5461
                     /direction=RIGHT
                     /note="pUC ori"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtgtc tagactgcag agggccctgc
      181 gtatgagtgc aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg
      241 accgaccccg acccactgga caagcaccca acccccattc cccaaattgc gcatccccta
      301 tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca
      361 ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa
      421 ggcgcgctga cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc
      481 gtccgcgccg ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac
      541 gggcgcgacc atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc
      601 agcggaggag tcgtgtcgtg cctgagagcg cagtcgagaa cagatctcta aaacaggtaa
      661 gtcccattaa tctccctatc agtgatagag aaggtctgaa gagtttactc cctatcagtg
      721 atagagatta attcctctac taaccttgtt catcttttct ttttttttct acaggtcctg
      781 ggtgattaac agcttaaggg cccggatccg gtaccgccac catgagtcgg ctggataaat
      841 ctaaagtcat aaacggcgct ctggaattac tcaatgaagt cggtatcgaa ggcctgacga
      901 caaggaaact cgctcaaaag ctgggagttg agcagcctac cctgtactgg cacgtgaaga
      961 acaagcgggc cctgctcgat gccctggcca tcgagatgct ggacaggcat catacccact
     1021 tctgccccct ggaaggcgag tcatggcaag actttctgcg gaacaacgcc aagtcattcc
     1081 gctgtgctct cctctcacat cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag
     1141 agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc
     1201 tggagaacgc actgtacgct ctgtccgccg tgggccactt tacactgggc tgcgtattgg
     1261 aggaacagga gcatcaagta gcaaaagagg aaagagagac acctaccacc gattctatgc
     1321 ccccacttct gagacaagca attgagctgt tcgaccggca gggagccgaa cctgccttcc
     1381 ttttcggcct ggaactaatc atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg
     1441 ggccggccga cgcccttgac gattttgact tagacatgct cccagccgat gcccttgacg
     1501 actttgacct tgatatgctg cctgctgacg ctcttgacga ttttgacctt gacatgctcc
     1561 ccgggtaaga attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg
     1621 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     1681 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     1741 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     1801 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     1861 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     1921 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     1981 cggggaaatc atcgtccttt ccttggctgc tcgcctatgt tgccacctgg attctgcgcg
     2041 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     2101 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     2161 ccctttgggc cgcctccccg catcgatacc gagcgctgct cgagagatct acgggtggca
     2221 tccctgtgac ccctccccag tgcctctcct ggccctggaa gttgccactc cagtgcccac
     2281 cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa
     2341 tattatgggg tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag
     2401 ggcctgcggg gtctattggg aaccaagctg gagtgcagtg gcacaatctt ggctcactgc
     2461 aatctccgcc tcctgggttc aagcgattct cctgcctcag cctcccgagt tgttgggatt
     2521 ccaggcatgc atgaccaggc tcagctaatt tttgtttttt tggtagagac ggggtttcac
     2581 catattggcc aggctggtct ccaactccta atctcaggtg atctacccac cttggcctcc
     2641 caaattgctg ggattacagg cgtgaaccac tgctcccttc cctgtccttc tgattttgta
     2701 ggtaaccacg tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc
     2761 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
     2821 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg
     2881 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
     2941 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     3001 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     3061 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     3121 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
     3181 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     3241 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt
     3301 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     3361 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
     3421 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     3481 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     3541 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     3601 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     3661 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     3721 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     3781 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     3841 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     3901 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     3961 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     4021 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     4081 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     4141 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     4201 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     4261 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     4321 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     4381 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     4441 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     4501 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     4561 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     4621 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     4681 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     4741 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     4801 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     4861 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     4921 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     4981 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     5041 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     5101 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     5161 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     5221 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     5281 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     5341 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     5401 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     5461 acatgt
//
