LOCUS Exported 5740 bp ds-DNA circular SYN 21-JUN-2017 DEFINITION hSynapsin driven Tet Transactivator with positive feedback loop for greater tTA expression at low vector doses. Cre dependent (DIO). ACCESSION . VERSION . KEYWORDS pAAV-ihSyn1-DIO-tTA SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 5740) AUTHORS . TITLE Direct Submission JOURNAL Exported Thursday, Jun 22, 2017 from SnapGene 3.1.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5740 /organism="recombinant plasmid" /mol_type="other DNA" misc_feature 1..141 /note="L-ITR" exon 156..640 /note="Human Synapsin I promoter" misc_feature 657..774 /note="synthetic intron" misc_feature 673..691 /note="TetO" misc_feature 709..727 /note="TetO" misc_feature 840..873 /note="lox2722" misc_feature 924..957 /note="loxP" CDS complement(966..1712) /codon_start=1 /note="tTA" /translation="MSRLDKSKVINGALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA DALDDFDLDMLPADALDDFDLDMLPG" misc_feature complement(1725..1758) /note="lox2722" misc_feature complement(1809..1842) /note="loxP" misc_feature 1857..2465 /note="WPRE" polyA_signal 2485..2963 /note="hGH polyA" misc_feature 3003..3143 /note="R-ITR" rep_origin 3235..3541 /direction=RIGHT /note="f1 Origin" misc_feature 4060..4917 /note="AmpR" rep_origin 5068..5735 /direction=RIGHT /note="pUC ori" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtgtgtc tagactgcag agggccctgc 181 gtatgagtgc aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg 241 accgaccccg acccactgga caagcaccca acccccattc cccaaattgc gcatccccta 301 tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca 361 ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa 421 ggcgcgctga cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc 481 gtccgcgccg ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac 541 gggcgcgacc atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc 601 agcggaggag tcgtgtcgtg cctgagagcg cagtcgagaa cagatctcta aaacaggtaa 661 gtcccattaa tctccctatc agtgatagag aaggtctgaa gagtttactc cctatcagtg 721 atagagatta attcctctac taaccttgtt catcttttct ttttttttct acaggtcctg 781 ggtgattaac agcttaaggg cccggatccg gtaccggatc ctctagagtc gactccggaa 841 taacttcgta taggatactt tatacgaagt tatgcagaat ggtagctgga ttgtagctgc 901 tattagcaat atgaaacctc ttaataactt cgtatagcat acattatacg aagttatggc 961 gcgccttacc cggggagcat gtcaaggtca aaatcgtcaa gagcgtcagc aggcagcata 1021 tcaaggtcaa agtcgtcaag ggcatcggct gggagcatgt ctaagtcaaa atcgtcaagg 1081 gcgtcggccg gcccgccgct ttcgcacttt agctgtttct ccaggccaca tatgattagt 1141 tccaggccga aaaggaaggc aggttcggct ccctgccggt cgaacagctc aattgcttgt 1201 ctcagaagtg ggggcataga atcggtggta ggtgtctctc tttcctcttt tgctacttga 1261 tgctcctgtt cctccaatac gcagcccagt gtaaagtggc ccacggcgga cagagcgtac 1321 agtgcgttct ccagggagaa gccttgctga cacaggaacg cgagctgatt ttccagggtt 1381 tcgtactgtt tctctgttgg gcgggtgccg agatgcactt tagccccgtc gcgatgtgag 1441 aggagagcac agcggaatga cttggcgttg ttccgcagaa agtcttgcca tgactcgcct 1501 tccagggggc agaagtgggt atgatgcctg tccagcatct cgatggccag ggcatcgagc 1561 agggcccgct tgttcttcac gtgccagtac agggtaggct gctcaactcc cagcttttga 1621 gcgagtttcc ttgtcgtcag gccttcgata ccgacttcat tgagtaattc cagagcgccg 1681 tttatgactt tagatttatc cagccgactc atggtggcgc tagcataact tcgtataaag 1741 tatcctatac gaagttattt gccttaaccc agaaattatc actgttattc tttagaatgg 1801 tgcaaagaat aacttcgtat aatgtatgct atacgaagtt atgaattcga tatcaagctt 1861 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat 1921 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct 1981 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag 2041 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc 2101 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc 2161 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct 2221 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg 2281 ctgctcgcct atgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg 2341 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg 2401 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga 2461 taccgagcgc tgctcgagag atctacgggt ggcatccctg tgacccctcc ccagtgcctc 2521 tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata aaattaagtt 2581 gcatcatttt gtctgactag gtgtccttct ataatattat ggggtggagg ggggtggtat 2641 ggagcaaggg gcaagttggg aagacaacct gtagggcctg cggggtctat tgggaaccaa 2701 gctggagtgc agtggcacaa tcttggctca ctgcaatctc cgcctcctgg gttcaagcga 2761 ttctcctgcc tcagcctccc gagttgttgg gattccaggc atgcatgacc aggctcagct 2821 aatttttgtt tttttggtag agacggggtt tcaccatatt ggccaggctg gtctccaact 2881 cctaatctca ggtgatctac ccaccttggc ctcccaaatt gctgggatta caggcgtgaa 2941 ccactgctcc cttccctgtc cttctgattt tgtaggtaac cacgtgcgga ccgagcggcc 3001 gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga 3061 ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga 3121 gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg 3181 cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta 3241 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 3301 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 3361 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 3421 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt 3481 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 3541 acactcaacc ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc 3601 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta 3661 acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc 3721 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca 3781 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg 3841 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat 3901 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga 3961 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa 4021 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt 4081 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg 4141 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg 4201 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg 4261 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag 4321 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca 4381 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg 4441 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc 4501 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg 4561 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg 4621 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac 4681 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg 4741 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg 4801 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact 4861 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa 4921 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt 4981 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag 5041 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct 5101 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt 5161 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg 5221 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct 5281 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc 5341 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg 5401 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa 5461 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg 5521 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg 5581 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga 5641 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt 5701 ttacggttcc tggccttttg ctggcctttt gctcacatgt //