LOCUS       Exported                5740 bp ds-DNA     circular SYN 21-JUN-2017
DEFINITION  hSynapsin driven Tet Transactivator with positive feedback loop for 
            greater tTA expression at low vector doses. Cre dependent (DIO).
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-ihSyn1-DIO-tTA
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jun 22, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    1..141
                     /note="L-ITR"
     exon            156..640
                     /note="Human Synapsin I promoter"
     misc_feature    657..774
                     /note="synthetic intron"
     misc_feature    673..691
                     /note="TetO"
     misc_feature    709..727
                     /note="TetO"
     misc_feature    840..873
                     /note="lox2722"
     misc_feature    924..957
                     /note="loxP"
     CDS             complement(966..1712)
                     /codon_start=1
                     /note="tTA"
                     /translation="MSRLDKSKVINGALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    complement(1725..1758)
                     /note="lox2722"
     misc_feature    complement(1809..1842)
                     /note="loxP"
     misc_feature    1857..2465
                     /note="WPRE"
     polyA_signal    2485..2963
                     /note="hGH polyA"
     misc_feature    3003..3143
                     /note="R-ITR"
     rep_origin      3235..3541
                     /direction=RIGHT
                     /note="f1 Origin"
     misc_feature    4060..4917
                     /note="AmpR"
     rep_origin      5068..5735
                     /direction=RIGHT
                     /note="pUC ori"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtgtc tagactgcag agggccctgc
      181 gtatgagtgc aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg
      241 accgaccccg acccactgga caagcaccca acccccattc cccaaattgc gcatccccta
      301 tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca
      361 ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa
      421 ggcgcgctga cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc
      481 gtccgcgccg ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac
      541 gggcgcgacc atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc
      601 agcggaggag tcgtgtcgtg cctgagagcg cagtcgagaa cagatctcta aaacaggtaa
      661 gtcccattaa tctccctatc agtgatagag aaggtctgaa gagtttactc cctatcagtg
      721 atagagatta attcctctac taaccttgtt catcttttct ttttttttct acaggtcctg
      781 ggtgattaac agcttaaggg cccggatccg gtaccggatc ctctagagtc gactccggaa
      841 taacttcgta taggatactt tatacgaagt tatgcagaat ggtagctgga ttgtagctgc
      901 tattagcaat atgaaacctc ttaataactt cgtatagcat acattatacg aagttatggc
      961 gcgccttacc cggggagcat gtcaaggtca aaatcgtcaa gagcgtcagc aggcagcata
     1021 tcaaggtcaa agtcgtcaag ggcatcggct gggagcatgt ctaagtcaaa atcgtcaagg
     1081 gcgtcggccg gcccgccgct ttcgcacttt agctgtttct ccaggccaca tatgattagt
     1141 tccaggccga aaaggaaggc aggttcggct ccctgccggt cgaacagctc aattgcttgt
     1201 ctcagaagtg ggggcataga atcggtggta ggtgtctctc tttcctcttt tgctacttga
     1261 tgctcctgtt cctccaatac gcagcccagt gtaaagtggc ccacggcgga cagagcgtac
     1321 agtgcgttct ccagggagaa gccttgctga cacaggaacg cgagctgatt ttccagggtt
     1381 tcgtactgtt tctctgttgg gcgggtgccg agatgcactt tagccccgtc gcgatgtgag
     1441 aggagagcac agcggaatga cttggcgttg ttccgcagaa agtcttgcca tgactcgcct
     1501 tccagggggc agaagtgggt atgatgcctg tccagcatct cgatggccag ggcatcgagc
     1561 agggcccgct tgttcttcac gtgccagtac agggtaggct gctcaactcc cagcttttga
     1621 gcgagtttcc ttgtcgtcag gccttcgata ccgacttcat tgagtaattc cagagcgccg
     1681 tttatgactt tagatttatc cagccgactc atggtggcgc tagcataact tcgtataaag
     1741 tatcctatac gaagttattt gccttaaccc agaaattatc actgttattc tttagaatgg
     1801 tgcaaagaat aacttcgtat aatgtatgct atacgaagtt atgaattcga tatcaagctt
     1861 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     1921 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     1981 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     2041 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     2101 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     2161 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     2221 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     2281 ctgctcgcct atgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     2341 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     2401 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga
     2461 taccgagcgc tgctcgagag atctacgggt ggcatccctg tgacccctcc ccagtgcctc
     2521 tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata aaattaagtt
     2581 gcatcatttt gtctgactag gtgtccttct ataatattat ggggtggagg ggggtggtat
     2641 ggagcaaggg gcaagttggg aagacaacct gtagggcctg cggggtctat tgggaaccaa
     2701 gctggagtgc agtggcacaa tcttggctca ctgcaatctc cgcctcctgg gttcaagcga
     2761 ttctcctgcc tcagcctccc gagttgttgg gattccaggc atgcatgacc aggctcagct
     2821 aatttttgtt tttttggtag agacggggtt tcaccatatt ggccaggctg gtctccaact
     2881 cctaatctca ggtgatctac ccaccttggc ctcccaaatt gctgggatta caggcgtgaa
     2941 ccactgctcc cttccctgtc cttctgattt tgtaggtaac cacgtgcgga ccgagcggcc
     3001 gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga
     3061 ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga
     3121 gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg
     3181 cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta
     3241 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     3301 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     3361 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     3421 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     3481 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     3541 acactcaacc ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc
     3601 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     3661 acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     3721 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     3781 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     3841 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     3901 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     3961 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     4021 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     4081 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     4141 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     4201 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     4261 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     4321 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     4381 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     4441 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     4501 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     4561 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     4621 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     4681 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     4741 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     4801 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     4861 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     4921 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     4981 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     5041 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     5101 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     5161 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     5221 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     5281 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     5341 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     5401 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     5461 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     5521 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     5581 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     5641 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     5701 ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
