LOCUS       Exported                5264 bp ds-DNA     circular SYN 21-JUN-2017
DEFINITION  hSynapsin1 driven mRuby2.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-hSyn1-mRuby2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jun 22, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5264
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..141
                     /note="L-ITR"
     exon            156..640
                     /note="Human Synapsin I promoter"
     CDS             653..1366
                     /codon_start=1
                     /note="mRuby2"
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     misc_feature    1381..1989
                     /note="WPRE"
     polyA_signal    2009..2487
                     /note="hGH polyA"
     misc_feature    2527..2667
                     /note="R-ITR"
     rep_origin      2759..3065
                     /direction=RIGHT
                     /note="f1 Origin"
     misc_feature    3584..4441
                     /note="AmpR"
     rep_origin      4592..5259
                     /direction=RIGHT
                     /note="pUC ori"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtgtc tagactgcag agggccctgc
      181 gtatgagtgc aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg
      241 accgaccccg acccactgga caagcaccca acccccattc cccaaattgc gcatccccta
      301 tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca
      361 ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa
      421 ggcgcgctga cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc
      481 gtccgcgccg ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac
      541 gggcgcgacc atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc
      601 agcggaggag tcgtgtcgtg cctgagagcg cagtcgagaa ggtaccgcca ccatggtgag
      661 caagggagag gaactcatca aggagaacat gcgcatgaag gtagtcatgg aagggtctgt
      721 gaatgggcat cagttcaagt gcacagggga aggtgaaggg aatccctaca tgggtactca
      781 gaccatgagg atcaaggtta ttgaaggtgg ccctctcccg tttgccttcg acattcttgc
      841 cactagcttc atgtatggca gtcggacctt catcaagtac cccaaaggca ttccagactt
      901 cttcaaacag tcctttccag agggcttcac atgggaaagg gtgacccgtt acgaggatgg
      961 tggtgtggtc acagtcatgc aggacactag tctggaggac ggatgtctgg tctatcacgt
     1021 gcaagtgaga ggggtgaact ttccctccaa tgggcctgtg atgcagaaga aaaccaaagg
     1081 gtgggagcca aacacagaga tgatgtatcc tgcagatgga ggactgagag gctatacgca
     1141 catggctctg aaggttgacg gaggaggcca cttgtcatgc tcatttgtga ccacgtacag
     1201 gagcaagaaa actgtcggca acatcaaaat gcctggcata catgcggttg atcaccgctt
     1261 ggaacgactg gaggagtctg acaacgagat gtttgtggta caacgggaac atgcagttgc
     1321 caagtttgct ggccttggcg gaggaatgga tgagctgtac aaatgagaat tcgatatcaa
     1381 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     1441 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     1501 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     1561 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     1621 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     1681 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     1741 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     1801 ttggctgctc gcctatgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     1861 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     1921 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     1981 tcgataccga gcgctgctcg agagatctac gggtggcatc cctgtgaccc ctccccagtg
     2041 cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta
     2101 agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg
     2161 gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa
     2221 ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa
     2281 gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc
     2341 agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc
     2401 aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg
     2461 tgaaccactg ctcccttccc tgtccttctg attttgtagg taaccacgtg cggaccgagc
     2521 ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca
     2581 ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga
     2641 gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc
     2701 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
     2761 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     2821 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     2881 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     2941 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     3001 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     3061 aacaacactc aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc
     3121 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     3181 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     3241 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     3301 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     3361 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     3421 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     3481 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     3541 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     3601 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     3661 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     3721 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     3781 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     3841 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     3901 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     3961 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     4021 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     4081 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     4141 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     4201 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     4261 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     4321 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     4381 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     4441 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     4501 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     4561 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     4621 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     4681 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     4741 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     4801 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4861 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4921 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4981 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     5041 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     5101 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     5161 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     5221 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
