LOCUS Exported 5261 bp ds-DNA circular SYN 21-JUN-2017 DEFINITION expression of membrane targeted mKate2.5 from a truncated version of the GFAP promoter. This plasmid contains a synthetic intron with two TetO sites for Tet inducible/repressible expression. 3 copies of a panel of 6 miRNA binding sites may help reduce off-target expression. ACCESSION . VERSION . KEYWORDS pAAV-GFAP-mKate2.5f SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5261) AUTHORS . TITLE Direct Submission JOURNAL Exported Thursday, Jun 22, 2017 from SnapGene 3.1.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5261 /organism="synthetic DNA construct" /mol_type="other DNA" misc_signal 1..145 /note="L-ITR" promoter 152..831 /note="GfABC1D enhancer/promoter" misc_feature 842..959 /note="synthetic intron" CDS 984..1742 /codon_start=1 /note="mKate2.5-f" /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK VVEGGPLPFAFDILATSFMYGSKTFINHPQGIPDFFKQSFPEGFTWERVTTYEDGGVLT ATQDTSLQDGCLIYNVKIRGVNFPANGPVMQKKTLGWEASTETLYPADGGLEGACDMAL KLVGGGHLICNLETTYRSKKPAKNLKMPGVYNVDRRLERIKEADNETYVEQHEVAVARY STGGAGDGGKKLNPPDESGPGCMSCKCVLS" misc_feature 1680..1739 /note="Ras farnesylation sequence" misc_feature 1749..2340 /note="WPRE" misc_feature 2360..2381 /note="miR1 BS" misc_feature 2393..2414 /note="miR122 BS" misc_feature 2421..2442 /note="miR-375 BS" misc_feature 2443..2464 /note="miR-196a BS" misc_feature 2470..2491 /note="miR-743 BS" misc_feature 2497..2519 /note="miR-10b BS" misc_feature 2541..2562 /note="miR1 BS" misc_feature 2574..2595 /note="miR122 BS" misc_feature 2602..2623 /note="miR-375 BS" misc_feature 2624..2645 /note="miR-196a BS" misc_feature 2651..2672 /note="miR-743 BS" misc_feature 2678..2700 /note="miR-10b BS" misc_feature 2722..2743 /note="miR1 BS" misc_feature 2755..2776 /note="miR122 BS" misc_feature 2783..2804 /note="miR-375 BS" misc_feature 2805..2826 /note="miR-196a BS" misc_feature 2832..2853 /note="miR-743 BS" misc_feature 2859..2881 /note="miR-10b BS" polyA_signal 2900..3132 /note="SV40 polyadenylation signal" misc_signal 3139..3283 /note="R-ITR" promoter 3489..3523 /note="P-Bla" /note="Beta Lactamase Promoter" CDS 3542..4381 /note="AMPr" /note="Ampicillin Resistance Gene" rep_origin 4610..5143 /direction=RIGHT /note="ORI" /note="Bacterial Origin of Replication" ORIGIN 1 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc 61 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag agagggagtg 121 gccaactcca tcactagggg ttcctactag taacatatcc tggtgtggag taggggacgc 181 tgctctgaca gaggctcggg ggcctgagct ggctctgtga gctggggagg aggcagacag 241 ccaggccttg tctgcaagca gacctggcag cattgggctg gccgcccccc agggcctcct 301 cttcatgccc agtgaatgac tcaccttggc acagacacaa tgttcggggt gggcacagtg 361 cctgcttccc gccgcacccc agcccccctc aaatgccttc cgagaagccc attgagcagg 421 gggcttgcat tgcaccccag cctgacagcc tggcatcttg ggataaaagc agcacagccc 481 cctaggggct gcccttgctg tgtggcgcca ccggcggtgg agaacaaggc tctattcagc 541 ctgtgcccag gaaaggggat caggggatgc ccaggcatgg acagtgggtg gcaggggggg 601 agaggagggc tgtctgcttc ccagaagtcc aaggacacaa atgggtgagg ggagagctct 661 ccccatagct gggctgcggc ccaaccccac cccctcaggc tatgccaggg ggtgttgcca 721 ggggcacccg ggcatcgcca gtctagccca ctccttcata aagccctcgt atcccaggag 781 cgagcagagc cagagcaggt tggagaggag acgcatcacc tctgctgctt gcctaaaaca 841 ggtaagtccc attaatctcc ctatcagtga tagagaaggt ctgaagagtt tactccctat 901 cagtgataga gattaattcc tctactaacc ttgttcatct tttctttttt tttctacagg 961 tcctgggtga ttaacagctt aagatggtga gcgagctgat taaggagaac atgcacatga 1021 agctgtacat ggagggcacc gtgaacaacc accacttcaa gtgcacatcc gagggcgaag 1081 gcaagcccta cgagggcacc cagaccatga gaatcaaggt cgtcgagggc ggccctctcc 1141 ccttcgcctt cgacatcctg gctaccagct tcatgtacgg cagcaaaacc ttcatcaacc 1201 accctcaggg catccccgac ttctttaagc agtccttccc tgagggcttc acatgggaga 1261 gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccagg 1321 acggctgcct catctacaac gtcaagatta gaggggtgaa cttcccagcc aacggccctg 1381 tgatgcagaa gaaaacactc ggctgggagg cctccaccga gacgctgtac cccgctgacg 1441 gcggcctgga aggcgcatgt gacatggccc tgaagctcgt gggcgggggc cacctgatct 1501 gcaacttgga gaccacatac agatccaaga aacccgctaa gaacctcaag atgcccggcg 1561 tctacaacgt ggacaggaga ctggaaagaa tcaaggaggc cgacaatgag acctacgtcg 1621 agcagcacga ggtggctgtg gccagatact ctactggtgg cgctggtgat ggaggtaaaa 1681 agctgaaccc tcctgatgag agtggccccg gctgcatgag ctgcaagtgt gtgctctcct 1741 gaggatccta atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac 1801 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt 1861 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat 1921 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca 1981 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc 2041 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg 2101 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct 2161 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct 2221 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt 2281 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct 2341 gaattctgca tgtttaaaca tacatacttc tttacattcc agatatctgc atcaaacacc 2401 attgtcacac tccatcgcga tcacgcgagc cgaacgaaca aacccaacaa catgaaacta 2461 cctagcgctt ctattcagca tgatgtcttt cgtatacaca aattcggttc tacagggtaa 2521 cctaggctgc atgtttaaac atacatactt ctttacattc cagatatctg catcaaacac 2581 cattgtcaca ctccatcgcg atcacgcgag ccgaacgaac aaacccaaca acatgaaact 2641 acctagcgct tctattcagc atgatgtctt tcgtatacac aaattcggtt ctacagggta 2701 acctaggctg catgtttaaa catacatact tctttacatt ccagatatct gcatcaaaca 2761 ccattgtcac actccatcgc gatcacgcga gccgaacgaa caaacccaac aacatgaaac 2821 tacctagcgc ttctattcag catgatgtct ttcgtataca caaattcggt tctacagggt 2881 aacctaggct gcaggtacct tcgagcagac atgataagat acattgatga gtttggacaa 2941 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct 3001 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt 3061 atgtttcagg ttcaggggga gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa 3121 tgtggtaaaa tcaagcttag gaacccctag tgatggagtt ggccactccc tctctgcgcg 3181 ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg 3241 cggcctcagt gagcgagcga gcgcgcagag agggagtggc caagctagcg ggcgattaag 3301 gaaagggcta gatcattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt 3361 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg 3421 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca 3481 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt 3541 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga 3601 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga 3661 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat 3721 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca 3781 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt 3841 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac 3901 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct 3961 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga 4021 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac 4081 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat 4141 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg 4201 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc 4261 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc 4321 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg 4381 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta 4441 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg 4501 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 4561 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 4621 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 4681 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa 4741 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 4801 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 4861 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 4921 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa 4981 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 5041 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 5101 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 5161 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtaataaa cacacacaca 5221 ccaacaaccg tggttggttg ttgtgttggt ttattctcga g //