LOCUS       Exported                5530 bp ds-DNA     circular SYN 21-JUN-2017
DEFINITION  Nuclear mRuby2 expressed from the DLX5/6 enhancer-synthetic 
            promoter/intron.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-mDlx-NLS-mRuby2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jun 22, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /note="L-ITR"
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     enhancer        170..699
                     /note="Dlx5/6 enhancer"
                     /note="Dlx enhancer"
     intron          860..992
                     /note="chimeric intron"
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             1089..1109
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     CDS             1119..1829
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /note="mRuby2"
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     misc_feature    1878..2465
                     /note="WPRE"
     polyA_signal    2633..2714
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_feature    2792..2933
                     /note="ITR"
     rep_origin      3008..3463
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3745..3849
                     /gene="bla"
                     /note="AmpR promoter"
     misc_feature    3850..4710
                     /note="Amp"
     CDS             3850..4710
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4881..5469
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtttaat taagaattct atacactcac
      181 agtggtttgg catatatttg gtgaaatttt ttaaggaaaa attagtgttg gtttcgatat
      241 atggtagctt tttctctaac ataatttgaa taattcagca aagccctact accagctgta
      301 cttctgcagc ctcttccatt ctttccagca ttataatttt ggttaatttt caattttagg
      361 tcctacgtct ctgcaatttg tgtatgaata acagaataat ttccctcttt tgtttcgcct
      421 ttcctgttcc tgaatctaaa taaagatggc tttttagtat taaaagtgga agaaaattac
      481 aggtaattat ctttgacggt aaaaacgctg taatcagcgg gctacatgaa aaattactct
      541 aattatggct gcatttaaga gaatggaaaa aaaccttctt gtggataaaa accttaaatt
      601 gtccccaatg tctgcttcaa attggatggc actgcagctg gaggctttgt tcagaattga
      661 tcctggggag ctacgaaccc aaagtttcac agtaggaagc ttgatatcga attcctgcag
      721 cccgggctgg gcataaaagt cagggcagag ccatctattg cttacatttg cttctagcct
      781 gcaggtcgag gagcgcagcc ttccagaagc agagcgcggc gccttaagct gcagaagttg
      841 gtcgtgaggc actgggcagg taagtatcaa ggttacaaga caggtttaag gagaccaata
      901 gaaactgggc ttgtcgagac agagaagact cttgcgtttc tgataggcac ctattggtct
      961 tactgacatc cactttgcct ttctctccac aggtgtccac tcccagttca attacagctc
     1021 ttaagaaact agtagccacc atgtctagtg atgatgaggc tactgctgac tctcaacatt
     1081 ctactcctcc aaaaaagaag agaaaggtag aagaccccat ggtgtctaag ggcgaagagc
     1141 tgatcaagga aaatatgcgt atgaaggtgg tcatggaagg ttcggtcaac ggccaccaat
     1201 tcaaatgcac aggtgaagga gaaggcaatc cgtacatggg aactcaaacc atgaggatca
     1261 aagtcatcga gggaggaccc ctgccatttg cctttgacat tcttgccacg tcgttcatgt
     1321 atggcagccg tacttttatc aagtacccga aaggcattcc tgatttcttt aaacagtcct
     1381 ttcctgaggg ttttacttgg gaaagagtta cgagatacga agatggtgga gtcgtcaccg
     1441 tcatgcagga caccagcctt gaggatggct gtctcgttta ccacgtccaa gtcagagggg
     1501 taaactttcc ctccaatggt cccgtgatgc agaagaagac caagggttgg gagcctaata
     1561 cagagatgat gtatccagca gatggtggtc tgaggggata cactcatatg gcactgaaag
     1621 ttgatggtgg tggccatctg tcttgctctt tcgtaacaac ttacaggtca aaaaagaccg
     1681 tcgggaacat caagatgccc ggtatccatg ccgttgatca ccgcctggaa aggttagagg
     1741 aaagtgacaa tgaaatgttc gtagtacaac gcgaacacgc agttgccaag ttcgccgggc
     1801 ttggtggtgg gatggacgag ctgtacaagt aaggcgcgcc acccctgcag ggaattcgat
     1861 atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     1921 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     1981 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     2041 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     2101 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     2161 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     2221 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     2281 tttccttggc tgctcgccta tgttgccacc tggattctgc gcgggacgtc cttctgctac
     2341 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     2401 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     2461 ccgcatcgat accgagcgct gctcgagaga tctgtgatag cggccatcaa gctggccgcg
     2521 actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     2581 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     2641 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     2701 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cagcttatcg
     2761 ataccgcatg cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac
     2821 tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc
     2881 gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct
     2941 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa
     3001 ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     3061 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     3121 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     3181 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
     3241 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     3301 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt
     3361 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     3421 aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt
     3481 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     3541 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     3601 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     3661 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     3721 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     3781 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     3841 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     3901 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     3961 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     4021 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     4081 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     4141 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     4201 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     4261 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     4321 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     4381 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     4441 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     4501 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     4561 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     4621 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     4681 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     4741 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     4801 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     4861 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     4921 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     4981 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     5041 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     5101 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     5161 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     5221 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     5281 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     5341 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     5401 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     5461 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     5521 gctcacatgt
//
