LOCUS       Exported                6167 bp ds-DNA     circular SYN 21-JUN-2017
DEFINITION  AAV-CAG-fDIO-mNeonGreen for flp-dependent, ubiquitous expression of 
            mNeonGreen.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-CAG-fDIO-mNeonGreen
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jun 22, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6167
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..141
                     /note="ITR"
     promoter        164..1263
                     /note="CAG"
     protein_bind    1303..1336
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FRT (minimal)"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    1387..1420
                     /note="frt*"
     CDS             complement(1429..2139)
                     /codon_start=1
                     /note="mNeonGreen"
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     protein_bind    complement(2152..2185)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FRT (minimal)"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    complement(2236..2269)
                     /note="frt*"
     misc_signal     2296..2883
                     /note="WPRE"
     polyA_signal    2912..3390
                     /note="hGH polyA"
     misc_feature    complement(3430..3570)
                     /note="LTR"
     CDS             3662..3968
                     /codon_start=1
                     /note="f1_origin"
                     /translation="ALSAAGVVVTRSVTATLASALAPAPFAFFPSFLATFAGFPRQALN
                     RGLPLGFRFSALRHLDPKKLDLGDGSRSGPSP**TVFRPLTLESTFFNSGLLFQTGT"
     CDS             4417..4445
                     /codon_start=1
                     /note="AmpR_promoter"
                     /translation="FKYVSAHET"
     CDS             4487..5347
                     /codon_start=1
                     /note="Ampicillin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             5502..6121
                     /codon_start=1
                     /note="pBR322_origin"
                     /translation="RSKDLLEILFFCA*SAACKQKNHRYQRWFVCRIKSYQLFFRR*LA
                     SAERRYQILSF*CSRS*ATTSRTL*HRLHTSLC*SCYQWLLPVAISRVLPGWTQDDSYR
                     IRRSGRAERGVRAHSPAWSERPTPN*DTYSVSYEKAPRFPKGERRTGIR*AAGSEQESA
                     RGSFQGETPGIFIVLSGFATSDLSVDFCDARQGGGAYGKTPAT"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaacac tagttattaa tagtaatcaa
      181 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      301 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      481 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
      541 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
      601 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggcgc gcgccaggcg
      661 gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc
      721 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata
      781 aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct
      841 ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga
      901 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg
      961 tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagcgccgg caggaaggaa
     1021 atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct
     1081 cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc
     1141 ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt
     1201 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
     1261 ttgcggccca acggtaccgg atcctctaga gtcgactccg gagaagttcc tattctctag
     1321 aaagtatagg aacttcgcag aatggtagct ggattgtagc tgctattagc aatatgaaac
     1381 ctcttagaag ttcctattct tcaaaaggta taggaacttc ggcgcgcctc acttgtacag
     1441 ctcgtccatg cccatcacat cggtaaaggc cttttgccac tccttgaagt tgagctcggt
     1501 cttggagtgc ttgagctccg tcttacggaa cacgtacatc ggctggttct tcagatagtt
     1561 agccgccatt ggcttggcaa aggtgtaggt ggtccgcgca gtgctccggt agcgcttgcc
     1621 atttccagtg gtgtaactcc acttaaaggt actgatgatg gttttgtcgt tggggtaagt
     1681 cttcttcgac ctgcaccagt ccgcagcggt cagcgagttg gtcatcacag gaccgtcagc
     1741 agggaaacca gtccccttca cctgggcctc tcctttgatg tggcttccct cgtaggtgta
     1801 gcggtagtta acagtaaggg aggcaccatc ttcaaactgc attgtgcgat ggacttggta
     1861 tccggagcca tctaccatgg cggcctggaa aggcgacatc ccgtcagggt agggcaggta
     1921 ctgatggaag ccatacccga tatgagggac cagaatccag ggggagaact ggaggtcacc
     1981 cttggtggac ttcaggttta actcctcata accatcattt ggattgccgg tgccctgacc
     2041 caccatgtca aagtccacac cgttgatgga gccaaagatg tgtaactcat gtgtcgctgg
     2101 gagagaggcc atgttatcct cctcgccctt gctcaccatg gtggcgctag cgaagttcct
     2161 atactttcta gagaatagga acttcttgcc ttaacccaga aattatcact gttattcttt
     2221 agaatggtgc aaagagaagt tcctatacct tttgaagaat aggaacttcg aattcgatat
     2281 caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     2341 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     2401 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     2461 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     2521 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     2581 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     2641 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     2701 tccttggctg ctcgcctatg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     2761 cccttcggcc ctcaatccag cggaccttcc tccccgcggc ctgctgccgg ctctgcggcc
     2821 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     2881 gcatcgatac cgagcgctgc tcgagagatc tacgggtggc atccctgtga cccctcccca
     2941 gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt cctaataaaa
     3001 ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg gtggaggggg
     3061 gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg ggtctattgg
     3121 gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc ctcctgggtt
     3181 caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg catgaccagg
     3241 ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc caggctggtc
     3301 tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct gggattacag
     3361 gcgtgaacca ctgctccctt ccctgtcctt ctgattttgt aggtaaccac gtgcggaccg
     3421 agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc
     3481 tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag
     3541 tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc
     3601 atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg
     3661 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     3721 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     3781 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     3841 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
     3901 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     3961 tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat
     4021 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     4081 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata
     4141 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     4201 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     4261 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     4321 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     4381 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     4441 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     4501 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     4561 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     4621 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     4681 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     4741 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     4801 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     4861 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     4921 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     4981 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     5041 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     5101 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     5161 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     5221 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     5281 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     5341 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     5401 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     5461 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     5521 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     5581 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     5641 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     5701 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     5761 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     5821 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     5881 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     5941 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6001 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6061 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6121 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//
