LOCUS       Exported                5893 bp ds-DNA     circular SYN 21-JUN-2017
DEFINITION  ubiquitous mNeonGreen expression.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-CAG-mNeonGreen
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jun 22, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5893
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..141
                     /note="L-ITR"
     misc_feature    151..1277
                     /note="KC103F"
     promoter        164..1263
                     /note="CAG"
     CDS             1285..1996
                     /codon_start=1
                     /note="mNeonGreen"
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     misc_feature    2010..2618
                     /note="WPRE"
     polyA_signal    2638..3116
                     /note="hGH polyA"
     misc_feature    3156..3296
                     /note="R-ITR"
     rep_origin      3388..3694
                     /direction=RIGHT
                     /note="f1 Origin"
     misc_feature    4213..5070
                     /note="AmpR"
     rep_origin      5221..5888
                     /direction=RIGHT
                     /note="pUC ori"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaacac tagttattaa tagtaatcaa
      181 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      301 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      481 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
      541 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
      601 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggcgc gcgccaggcg
      661 gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc
      721 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata
      781 aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct
      841 ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga
      901 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg
      961 tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagcgccgg caggaaggaa
     1021 atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct
     1081 cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc
     1141 ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt
     1201 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
     1261 ttgcggccca acggtaccgc caccatggtg agcaagggcg aggaggataa catggcctct
     1321 ctcccagcga cacatgagtt acacatcttt ggctccatca acggtgtgga ctttgacatg
     1381 gtgggtcagg gcaccggcaa tccaaatgat ggttatgagg agttaaacct gaagtccacc
     1441 aagggtgacc tccagttctc cccctggatt ctggtccctc atatcgggta tggcttccat
     1501 cagtacctgc cctaccctga cgggatgtcg cctttccagg ccgccatggt agatggctcc
     1561 ggataccaag tccatcgcac aatgcagttt gaagatggtg cctcccttac tgttaactac
     1621 cgctacacct acgagggaag ccacatcaaa ggagaggccc aggtgaaggg gactggtttc
     1681 cctgctgacg gtcctgtgat gaccaactcg ctgaccgctg cggactggtg caggtcgaag
     1741 aagacttacc ccaacgacaa aaccatcatc agtaccttta agtggagtta caccactgga
     1801 aatggcaagc gctaccggag cactgcgcgg accacctaca cctttgccaa gccaatggcg
     1861 gctaactatc tgaagaacca gccgatgtac gtgttccgta agacggagct caagcactcc
     1921 aagaccgagc tcaacttcaa ggagtggcaa aaggccttta ccgatgtgat gggcatggac
     1981 gagctgtaca agtgagaatt cgatatcaag cttatcgata atcaacctct ggattacaaa
     2041 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     2101 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     2161 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     2221 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     2281 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     2341 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     2401 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctatgttgc cacctggatt
     2461 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     2521 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     2581 cggatctccc tttgggccgc ctccccgcat cgataccgag cgctgctcga gagatctacg
     2641 ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag
     2701 tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct
     2761 tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa
     2821 cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc
     2881 tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt
     2941 tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg
     3001 gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt
     3061 ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctga
     3121 ttttgtaggt aaccacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc
     3181 cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg
     3241 cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg
     3301 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag
     3361 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     3421 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     3481 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     3541 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
     3601 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     3661 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc gggctattct
     3721 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     3781 caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc
     3841 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     3901 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     3961 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     4021 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     4081 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     4141 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     4201 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     4261 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     4321 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     4381 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     4441 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     4501 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     4561 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     4621 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     4681 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     4741 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     4801 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     4861 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     4921 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     4981 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     5041 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     5101 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     5161 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5221 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     5281 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     5341 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     5401 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     5461 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     5521 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     5581 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     5641 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     5701 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     5761 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     5821 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     5881 tttgctcaca tgt
//
