LOCUS       Exported                5261 bp ds-DNA     circular SYN 21-JUN-2017
DEFINITION  hSynapsin1 driven mNeonGreen.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-hSyn1-mNeonGreen
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jun 22, 2017 from SnapGene 3.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5261
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..141
                     /note="L-ITR"
     exon            156..640
                     /note="Human Synapsin I promoter"
     CDS             653..1363
                     /codon_start=1
                     /note="mNeonGreen"
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     misc_feature    1378..1986
                     /note="WPRE"
     polyA_signal    2006..2484
                     /note="hGH polyA"
     misc_feature    2524..2664
                     /note="R-ITR"
     rep_origin      2756..3062
                     /direction=RIGHT
                     /note="f1 Origin"
     misc_feature    3581..4438
                     /note="AmpR"
     rep_origin      4589..5256
                     /direction=RIGHT
                     /note="pUC ori"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtgtc tagactgcag agggccctgc
      181 gtatgagtgc aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg
      241 accgaccccg acccactgga caagcaccca acccccattc cccaaattgc gcatccccta
      301 tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca
      361 ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa
      421 ggcgcgctga cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc
      481 gtccgcgccg ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac
      541 gggcgcgacc atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc
      601 agcggaggag tcgtgtcgtg cctgagagcg cagtcgagaa ggtaccgcca ccatggtgag
      661 caagggcgag gaggataaca tggcctctct cccagcgaca catgagttac acatctttgg
      721 ctccatcaac ggtgtggact ttgacatggt gggtcagggc accggcaatc caaatgatgg
      781 ttatgaggag ttaaacctga agtccaccaa gggtgacctc cagttctccc cctggattct
      841 ggtccctcat atcgggtatg gcttccatca gtacctgccc taccctgacg ggatgtcgcc
      901 tttccaggcc gccatggtag atggctccgg ataccaagtc catcgcacaa tgcagtttga
      961 agatggtgcc tcccttactg ttaactaccg ctacacctac gagggaagcc acatcaaagg
     1021 agaggcccag gtgaagggga ctggtttccc tgctgacggt cctgtgatga ccaactcgct
     1081 gaccgctgcg gactggtgca ggtcgaagaa gacttacccc aacgacaaaa ccatcatcag
     1141 tacctttaag tggagttaca ccactggaaa tggcaagcgc taccggagca ctgcgcggac
     1201 cacctacacc tttgccaagc caatggcggc taactatctg aagaaccagc cgatgtacgt
     1261 gttccgtaag acggagctca agcactccaa gaccgagctc aacttcaagg agtggcaaaa
     1321 ggcctttacc gatgtgatgg gcatggacga gctgtacaag tgagaattcg atatcaagct
     1381 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     1441 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     1501 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     1561 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     1621 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     1681 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     1741 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     1801 gctgctcgcc tatgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     1861 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     1921 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     1981 ataccgagcg ctgctcgaga gatctacggg tggcatccct gtgacccctc cccagtgcct
     2041 ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt
     2101 tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta
     2161 tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca
     2221 agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg
     2281 attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc
     2341 taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac
     2401 tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga
     2461 accactgctc ccttccctgt ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc
     2521 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     2581 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
     2641 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt
     2701 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
     2761 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     2821 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     2881 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     2941 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     3001 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     3061 aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc
     3121 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     3181 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     3241 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     3301 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     3361 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     3421 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     3481 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     3541 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     3601 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     3661 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     3721 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     3781 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     3841 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     3901 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     3961 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     4021 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     4081 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     4141 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     4201 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     4261 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     4321 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     4381 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     4441 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     4501 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     4561 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     4621 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     4681 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4741 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4801 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4861 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4921 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4981 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     5041 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     5101 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     5161 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     5221 tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
