LOCUS       pDB016_actual           3981 bp    DNA     circular UNA 05-JUL-2017
DEFINITION  .
ACCESSION   urn.local...5o-7q3iana
VERSION     urn.local...5o-7q3iana
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     rep_origin      1..588
                     /label="ColE1 origin of replication"
     CDS             complement(1016..1207)
                     /note="Rop"
                     /label="Rop CDS"
     terminator      1318..1817
                     /created_by="User"
                     /label="long terminator"
     Promoter        1827..1846
                     /created_by="User"
                     /label="T7 Prom"
     RBS             1868..1882
                     /created_by="User"
                     /label="SD8 RBS"
     CDS             1883..2977
                     /created_by="User"
                     /label="pIII-neg"
     terminator      complement(3002..3043)
                     /note="tetA/orfL bidirectional terminator"
                     /note="termination of bla transcript is slightly weaker
                     than opposite direction"
                     /label="tetA/orfL bidirectional terminator"
     CDS             complement(3057..3848)
                     /dnas_title="aadA"
                     /vntifkey=4
                     /label="aadA"
     promoter        3849..3933
                     /note="aadA promoter"
                     /label="aadA promoter"
ORIGIN      
        1 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
       61 gcggtggttt gtttgccgga tcaagagcta ccacctcttt ttccgaaggt aactggcttc
      121 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
      181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
      241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
      301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
      361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
      421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
      481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
      541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
      601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
      661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
      721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
      781 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
      841 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
      901 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      961 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1021 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     1081 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     1141 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     1201 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     1261 aacgagagag gatgctcacg atacgggtta ctgatgatga acaagaggac atccggtaag
     1321 gccaaatagg ggttcataag gtacggcccg tactgaaccc gggtaggctg cttgagccag
     1381 tgagcgattg ctggcctaga tgaatgactg tccacgacag aacccggctt atcggtcagt
     1441 ttcacctgat ttacgtaaaa acccgcttcg gcgggttttt gcttttggag gggcagaaag
     1501 atgaatgact gtccacgacg ctatacccaa aagaaagcgg cttatcggtc agtttcacct
     1561 ggtttacgta aaaacccgct tcggcgggtt tttgcttttg gaggggcaga aagatgaatg
     1621 actgtccacg acactatacc caaaagaaag cggcttatcg gtcagtttca cctgttttac
     1681 gtaaaaaccc gcttcggcgg gtttttactt ttggaggggc agaaagatga atgactgtcc
     1741 acgacactat acccaaaaga aagcggctta tcggtcagtt ttacctgatg tacgtaataa
     1801 accgttccgg cgggtttggg acaaattaat acgactcact atagggtgaa agacctgcag
     1861 gtgcagtaag gaggaaaaaa aaatgaaaaa attattattc gcaattcctt tagttgttcc
     1921 tttctattct cactccgctg aaactgttca tcaccatcac catcacgctg aaactgttga
     1981 aagttgttta gcaaaacccc atacagaaaa ttcatttact aacgtctgga aagacgacaa
     2041 aactttagat cgttacgcta actatgaggg ctgtctgtgg aatgctacag gcgttgtagt
     2101 ttgtactggt gacgaaactc agtgttacgg tacatgggtt cctattgggc ttgctatccc
     2161 tgaaaatgag ggtggtggct ctgagggtgg cggttctgag ggtggcggtt ctgagggtgg
     2221 cggtactaaa cctcctgagt acggtgatac acctattccg ggctatactt atatcaaccc
     2281 tctcgacggc acttatccgc ctggtactga gcaaaacccc gctaatccta atccttctct
     2341 tgaggagtct cagcctctta atactttcat gtttcagaat aataggttcc gaaataggca
     2401 gggggcatta actgtttata cgggcactgt tactcaaggc actgaccccg ttaaaactta
     2461 ttaccagtac actcctgtat catcaaaagc catgtatgac gcttactgga acggtaaatt
     2521 cagagactgc gctttccatt ctggctttaa tgaggatcca ttcgtttgtg aatatcaagg
     2581 ccaatcgtct gacctgcctc aacctcctgt caatgctggc ggcggctctg gtggtggttc
     2641 tggtggcggc tctgagggtg gtggctctga gggtggcggt tctgagggtg gcggctctga
     2701 gggaggcggt tccggtggtg gctcttccca aatggctcaa gtcggtgacg gtgataattc
     2761 acctttaatg aataatttcc gtcaatattt accttccctc cctcaatcgg ttgaatgtcg
     2821 cccttttgtc tttggcgctg gtaaacctta cgagttcagt atcgactgcg ataagatcaa
     2881 cctgttccgc ggtgtctttg cgtttctttt atatgttgcc acctttatgt atgtattttc
     2941 tacgtttgct aacatactgc gtaataagga gtcttaaact taattaacgg cactcctcag
     3001 caaatataat gaccctcttg ataacccaag agggcatttt ttaatgccca tggcgtttat
     3061 ttgccgacta ccttggtgat ctcgcctttc acgtagtgga caaattcttc caactgatct
     3121 gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag cctgtctagc ttcaagtatg
     3181 acgggctgat actgggccgg caggcgctcc attgcccagt cggcagcgac atccttcggc
     3241 gcgattttgc cggttactgc gctgtaccaa atgcgggaca acgtaagcac tacatttcgc
     3301 tcatcgccag cccagtcggg cggcgagttc catagcgtta aggtttcatt tagcgcctca
     3361 aatagatcct gttcaggaac cggatcaaag agttcctccg ccgctggacc taccaaggca
     3421 acgctatgtt ctcttgcttt tgtcagcaag atagccagat caatgtcgat cgtggctggc
     3481 tcgaagatac ctgcaagaat gtcattgcgc tgccattctc caaattgcag ttcgcgctta
     3541 gctggataac gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg
     3601 agaatctcgc tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc
     3661 cgcgttgttt catcaagcct tacggtcacc gtaaccagca aatcaatatc actgtgtggc
     3721 ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga
     3781 tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc gatcaccgct
     3841 tccctcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     3901 agcggataca tatttgaatg tatttagaaa aatattaatt gcgttgcgct cactgcccgc
     3961 tttccaggcc aaataggccg t
//
