LOCUS Exported 3506 bp ds-DNA circular SYN 26-JUN-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pRSETa_mEos3.1_69T SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3506) AUTHORS . TITLE Direct Submission JOURNAL Exported Monday, Jun 26, 2017 from SnapGene 3.3.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3506 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 13..30 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 34..66 /codon_start=1 /product="leader peptide from bacteriophage T7 gene 10" /label=T7 tag (gene 10 leader) /note="promotes efficient translation in E. coli" /translation="MASMTGGQQMG" CDS 70..93 /codon_start=1 /product="Xpress(TM) epitope tag, including an enterokinase recognition and cleavage site" /label=Xpress(TM) tag /translation="DLYDDDDK" CDS 109..789 /codon_start=1 /product="improved monomeric variant of green-to-red photoswitchable fluorescent protein EosFP (Zhang et al., 2012)" /label=pr-mEos3.1 (mEos3.1-A69T) /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK EGGPLPFAFDILTTAFHYGNRVFTKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD NARR" misc_feature 314..315 /label=A69T terminator 866..913 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" rep_origin 1010..1465 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 1546..1650 /gene="bla" /label=AmpR promoter rep_origin 2682..3270 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 atgcggggtt ctcatcatca tcatcatcat ggtatggcta gcatgactgg tggacagcaa 61 atgggtcggg atctgtacga cgatgacgat aaggatcgat ggggatccat gagtgcgatt 121 aagccagaca tgaagatcaa actccgtatg gaaggcaacg taaacgggca ccactttgtg 181 atcgacggag atggtacagg caagcctttt gagggaaaac agagtatgga tcttgaagtc 241 aaagagggcg gacctctgcc ttttgccttt gatatcctga ccactgcatt ccattacggc 301 aacagggtat tcaccaaata tccagacaac atacaagact attttaagca gtcgtttcct 361 aaggggtatt cgtgggaacg aagcttgact ttcgaagacg ggggcatttg caatgccaga 421 aacgacataa caatggaagg ggacactttc tataataaag ttcgatttta tggtaccaac 481 tttcccgcca atggtccagt tatgcagaag aagacgctga aatgggagcc ctccactgag 541 aaaatgtatg tgcgtgatgg agtgctgacg ggtgatgttg agatggcttt gttgcttgaa 601 ggaaatgccc attaccgatg tgacttcaga actacttaca aagctaagga gaagggtgtc 661 aagttaccag gcgcccactt tgtggaccac tgcattgaga ttttaagcca tgacaaagat 721 tacaacaagg ttaagctgta tgagcatgct gttgctcatt ctggattgcc tgacaatgcc 781 agacgataag aattcgaagc ttgatccggc tgctaacaaa gcccgaaagg aagctgagtt 841 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt 901 gaggggtttt ttgctgaaag gaggaactat atccggatct ggcgtaatag cgaagaggcc 961 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt 1021 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 1081 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 1141 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag agctttacgg 1201 cacctcgacc gcaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga 1261 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc 1321 caaactggaa caacactcaa ccctatcgcg gtctattctt ttgatttata agggattttg 1381 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaatatttaa cgcgaatttt 1441 aacaaaatat taacgtttac aatttcgcct gatgcggtat tttctcctta cgcatctgtg 1501 cggtatttca caccgcatac aggtggcact tttcggggaa atgtgcgcgg aacccctatt 1561 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa 1621 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt 1681 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa 1741 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac 1801 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt 1861 aaagttctgc tatgtgatac actattatcc cgtattgacg ccgggcaaga gcaactcggt 1921 cgccccccgc ggtattctca gaatgacttg gttgagtact caccagtcac agaaaagcat 1981 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac 2041 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg 2101 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc 2161 ataccaaacg acgagagtga caccacgatg cctgtagcaa tgccaacaac gttgcgcaaa 2221 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctgaatggag 2281 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct 2341 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat 2401 ggtaagcgct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa 2461 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac 2521 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 2581 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 2641 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 2701 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 2761 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 2821 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 2881 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 2941 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 3001 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 3061 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 3121 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 3181 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 3241 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 3301 ctgggctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 3361 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag 3421 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc 3481 gcgcgttggc cgattcatta atgcag //