LOCUS       pADMU4                  3795 bp ds-DNA     circular     27-OCT-2016
DEFINITION  pRSF-1b (kan).
SOURCE      
  ORGANISM  
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             complement(705..1520)
                     /vntifkey="4"
                     /label=KanR
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(2573..3655)
                     /vntifkey="4"
                     /note="Wildtype lacI gene"
                     /label=lacI
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        3779..3795
                     /vntifkey="30"
                     /label=T7 Promoter
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     protein_bind    5..25
                     /vntifkey="31"
                     /label=lacO
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      428..475
                     /vntifkey="43"
                     /label=T7 Terminator
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      1628..2377
                     /vntifkey="33"
                     /label=RSF1030 origin
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    71..394
                     /label=GamS-6xHis construct
                     /ApEinfo_fwdcolor=#70b867
                     /ApEinfo_revcolor=#70b867
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
       61 gagatatacc atgggaaacg cttattacat tcaggatcgt cttgaggctc agagctgggc
      121 gcgtcactac cagcagctcg cccgtgaaga gaaagaggca gaactggcag acgacatgga
      181 aaaaggcctg ccccagcacc tgtttgaatc gctatgcatc gatcatttgc aacgccacgg
      241 ggccagcaaa aaatccatta cccgtgcgtt tgatgacgat gttgagtttc aggagcgcat
      301 ggcagaacac atccggtaca tggttgaaac cattgctcac caccaggttg atattgattc
      361 agaggtactc gagcaccacc accaccacca ctaattaacc taggctgctg ccaccgctga
      421 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
      481 acctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa
      541 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga caagctgacg
      601 accgggtctc cgcaagtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
      661 ttttctaaat acattcaaat atgtatccgc tcatgaatta attcttagaa aaactcatcg
      721 agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa
      781 agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc
      841 tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg
      901 tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat
      961 ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca
     1021 tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga
     1081 aatacgcggt cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg
     1141 aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg
     1201 aatgctgttt tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata
     1261 aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca
     1321 tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg
     1381 ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat
     1441 ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctaga gcaagacgtt
     1501 tcccgttgaa tatggctcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     1561 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggcatg
     1621 cagcgctctt ccgcttcctc gctcactgac tcgctacgct cggtcgttcg actgcggcga
     1681 gcggtgtcag ctcactcaaa agcggtaata cggttatcca cagaatcagg ggataaagcc
     1741 ggaaagaaca tgtgagcaaa aagcaaagca ccggaagaag ccaacgccgc aggcgttttt
     1801 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagcc agaggtggcg
     1861 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     1921 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     1981 ggcgctttct catagctcac gctgttggta tctcagttcg gtgtaggtcg ttcgctccaa
     2041 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     2101 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccattggtaa
     2161 ctgatttaga ggactttgtc ttgaagttat gcacctgtta aggctaaact gaaagaacag
     2221 attttggtga gtgcggtcct ccaacccact taccttggtt caaagagttg gtagctcagc
     2281 gaaccttgag aaaaccaccg ttggtagcgg tggtttttct ttatttatga gatgatgaat
     2341 caatcggtct atcaagtcaa cgaacagcta ttccgttact ctagatttca gtgcaattta
     2401 tctcttcaaa tgtagcacct gaagtcagcc ccatacgata taagttgtaa ttctcatgtt
     2461 agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg
     2521 tcgagatccc ggtgcctaat gagtgagcta acttacatta attgcgttgc gctcactgcc
     2581 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     2641 gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt gagacgggca
     2701 acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg
     2761 tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata taacatgagc
     2821 tgtcttcggt atcgtcgtat cccactaccg agatgtccgc accaacgcgc agcccggact
     2881 cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg
     2941 gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt
     3001 cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag
     3061 ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt
     3121 gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa
     3181 tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg
     3241 cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga
     3301 cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta
     3361 ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa
     3421 tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt
     3481 tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt
     3541 ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg
     3601 tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca
     3661 ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgcc
     3721 attcgatggt gtccgggatc tcgacgctct cccttatgcg actcctgcat taggaaatta
     3781 atacgactca ctata    
//
