LOCUS       AAV_pCAG_Flex_mRuby3        6455 bp    DNA     circular     13-FEB-2017
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11633309|
COMMENT     VNTDBDATE|-11633308|
COMMENT     LSOWNER|
COMMENT     VNTNAME|AAV_pCAG_Flex_mRuby3|
COMMENT     VNTAUTHORNAME|Rylan Larsen|
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COMMENT                             #262=(CScratch 
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COMMENT                      #293=(CGroupWidget 
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COMMENT                                   #302#)) (CObjectList)) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "bGH PA terminator" "@N" 1 -9.49799 -13.9386 
COMMENT                                   0 -10 8.11733 1.04901 #305#)) (CObjectList))) 
COMMENT                     (CObjectList)) 
COMMENT               #307=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -1019 100) 
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COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #309=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -949 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
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FEATURES             Location/Qualifiers
     rep_origin      5790..6409
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="33"
                     /label=pBR322_origin
     gene            4775..5635
                     /gene="Ampicillin"
                     /ApEinfo_fwdcolor="#ff797d"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="60"
     promoter        4705..4733
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="30"
                     /label=AmpR_promoter
     misc_feature    4524..4546
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=pGEX_3_primer
     rep_origin      3950..4256
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="33"
                     /label=f1_origin
     terminator      3485..3688
                     /ApEinfo_fwdcolor="#9d1b1c"
                     /ApEinfo_revcolor="#9d1b1c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="43"
                     /label=bGH_PA_terminator
     misc_feature    complement(3482..3499)
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=BGH_rev_primer
     misc_feature    2802..2835
                     /ApEinfo_label="loxP"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=loxP(3)
     misc_feature    complement(2758..2791)
                     /ApEinfo_label="loxP"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=loxP(2)
     misc_feature    1983..2016
                     /ApEinfo_label="loxP"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=loxP(1)
     misc_feature    1939..1972
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=loxP
     misc_feature    1843..1862
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=pCAG_F_primer
     misc_feature    238..525
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=CAG_enhancer
     misc_feature    complement(2027..2029)
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=Stop\Codon
     misc_feature    complement(2741..2746)
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=kozak
     misc_feature    complement(2030..2740)
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=mRuby3
     misc_feature    4775..5635
                     /vntifkey="21"
                     /label=AmpR
     promoter        233..816
                     /vntifkey="29"
                     /label=CAG\Promoter
     intron          818..1835
                     /vntifkey="15"
                     /label=Chimeric\Intron
     misc_feature    2859..3447
                     /vntifkey="21"
                     /label=WPRE
     polyA_signal    3488..3695
                     /vntifkey="25"
                     /label=bGH\Poly(A)\signal
     misc_feature    3718..3858
                     /vntifkey="21"
                     /label=AAV2\ITR
     misc_feature    1..141
                     /vntifkey="21"
                     /label=AAV2\ITR
BASE COUNT     1268 a      1775 c      1825 g      1587 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca cgcgaaacaa ttctgcagga atctagttat 
      181 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca 
      241 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca 
      301 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg 
      361 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg 
      421 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc 
      481 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc 
      541 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt 
      601 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg 
      661 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg 
      721 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg 
      781 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggga gtcgctgcga cgctgccttc 
      841 gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt 
      901 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg 
      961 tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg 
     1021 gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg 
     1081 ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt 
     1141 gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg 
     1201 ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg 
     1261 gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga gttgctgagc 
     1321 acggcccggc ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg 
     1381 gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg 
     1441 gctcggggga ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc 
     1501 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa 
     1561 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga 
     1621 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc 
     1681 gccgtcccct tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg 
     1741 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg 
     1801 ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg 
     1861 tgctgtctca tcattttggc aaagaattga tttgataccg cgggccccgc gtggacggta 
     1921 cccttcaggt cgacgtcgat aacttcgtat agcatacatt atacgaagtt atccgaatcg 
     1981 caataacttc gtataggata ctttatacga agttatttgg cgcgcctgac ttgtacagct 
     2041 cgtccatgcc accaccaagg ttgctgtatt tggcaactgc cacttctctt tgcactacgt 
     2101 aggtttcatt gtcactctcc tcgatccttt ccaggcggtg atcaacggca tggacaccgg 
     2161 gcatcttgat gttcccgacg gtcttttttg acctgtaagt tgtcacgaag ttgcagtgca 
     2221 gatggccacc accatcaact ttcagtgcga tgtcagtgta tcctctcaga ccaccatctg 
     2281 ctggatacat catctctgta ttaggctccc aacccttggt cttcttctgc atcacgggac 
     2341 cattggaggg aaagtttacc cctctgacct tgacgttgta gacgagctcg ccatcctcaa 
     2401 ggctggtgtc ctgcgtgacg gtgacgactc caccatcttc gtatctcgta actctttccc 
     2461 aagtaaaacc ctcaggaaag gactgtttaa agaaatcagg gatgtcggcc gggtacttga 
     2521 taaaggtacg gctgccatac atgaacgacg tggcaagaat gtcaaaggca aatggcaggg 
     2581 gtcctccctc gatgactttg atcctcatgg tttgcactcc ctcgtacggt ctgccttctc 
     2641 cttcacctgt gcatttgaat tggtggccgt tgaccgaacc ttccatgacc accttcatac 
     2701 gcatattttc cttgatcagc tcttcgccct tagacaccat gccaccggcc ggccttgata 
     2761 acttcgtata atgtatgcta tacgaagtta tccgaatcgc aataacttcg tataaagtat 
     2821 cctatacgaa gttatcgaag atcatcaagc ttatcgataa tcaacctctg gattacaaaa 
     2881 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg 
     2941 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct 
     3001 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg 
     3061 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct 
     3121 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg 
     3181 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg 
     3241 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctatgttgcc acctggattc 
     3301 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc 
     3361 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc 
     3421 ggatctccct ttgggccgcc tccccgcatc gataccgagc gctgctcgag agatcgatct 
     3481 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc 
     3541 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg 
     3601 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg 
     3661 gaggattggg aagacaatag caggcatgct ggggacacgt gcggaccgag cggccgcagg 
     3721 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg 
     3781 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag 
     3841 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta 
     3901 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc 
     3961 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc 
     4021 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct 
     4081 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa 
     4141 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc 
     4201 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact 
     4261 caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg 
     4321 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt 
     4381 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc 
     4441 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc 
     4501 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc 
     4561 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat 
     4621 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc 
     4681 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg 
     4741 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc 
     4801 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt 
     4861 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct 
     4921 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac 
     4981 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact 
     5041 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa 
     5101 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga 
     5161 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt 
     5221 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga 
     5281 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg 
     5341 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat 
     5401 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat 
     5461 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc 
     5521 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga 
     5581 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc 
     5641 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag 
     5701 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc 
     5761 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 
     5821 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 
     5881 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 
     5941 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 
     6001 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 
     6061 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 
     6121 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 
     6181 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 
     6241 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 
     6301 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 
     6361 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 
     6421 gttcctggcc ttttgctggc cttttgctca catgt 
//
