LOCUS       AAV_pCAG_FLEX_mScarlet        6440 bp    DNA     circular     29-JUN-2017
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|-11734178|
COMMENT     VNTDBDATE|-11829015|
COMMENT     LSOWNER|
COMMENT     VNTNAME|AAV_pCAG_FLEX_mScarlet|
COMMENT     VNTAUTHORNAME|Rylan Larsen|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "AAV_pCAG_FLEX_mScarlet" 0 6440 
COMMENT       (CDBMol 0 0 1 3704 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 0 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "ApaLI" "AvaI" "BamHI" "ClaI" "EcoRI" "HindIII" "NcoI" 
COMMENT         "PstI" "SmaI" "XmaI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 6440 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #0=(CFSignal (CObList) "pBR322_origin" 33 0 0 5775 6394 1 (CStringList) 
COMMENT            (CStringList "/ApEinfo_fwdcolor=\"#999999\"" 
COMMENT             "/ApEinfo_revcolor=\"#999999\"" 
COMMENT             "/ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT             "width 5 offset 0\"") 1 1 1 1 "6532..7151") 
COMMENT        #1=(CFSignal (CObList) "AmpR_promoter" 30 0 0 4690 4718 1 (CStringList) 
COMMENT            (CStringList "/ApEinfo_fwdcolor=\"#346ee0\"" 
COMMENT             "/ApEinfo_revcolor=\"#346ee0\"" 
COMMENT             "/ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT             "width 5 offset 0\"") 1 1 1 1 "5447..5475") 
COMMENT        #2=(CFSignal (CObList) "pGEX_3_primer" 21 0 0 4509 4531 1 (CStringList) 
COMMENT            (CStringList "/ApEinfo_fwdcolor=\"#7eff74\"" 
COMMENT             "/ApEinfo_revcolor=\"#7eff74\"" 
COMMENT             "/ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT             "width 5 offset 0\"") 1 1 1 1 "5266..5288") 
COMMENT        #3=(CFSignal (CObList) "f1_origin" 33 0 0 3935 4241 1 (CStringList) 
COMMENT            (CStringList "/ApEinfo_fwdcolor=\"#999999\"" 
COMMENT             "/ApEinfo_revcolor=\"#999999\"" 
COMMENT             "/ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT             "width 5 offset 0\"") 1 1 1 1 "4692..4998") 
COMMENT        #4=(CFSignal (CObList) "bGH_PA_terminator" 43 0 0 3470 3673 1 
COMMENT            (CStringList) 
COMMENT            (CStringList "/ApEinfo_fwdcolor=\"#9d1b1c\"" 
COMMENT             "/ApEinfo_revcolor=\"#9d1b1c\"" 
COMMENT             "/ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT             "width 5 offset 0\"") 1 1 1 1 "4227..4430") 
COMMENT        #5=(CFSignal (CObList) "BGH_rev_primer" 21 0 1 3467 3484 1 (CStringList) 
COMMENT            (CStringList "/ApEinfo_fwdcolor=\"#7eff74\"" 
COMMENT             "/ApEinfo_revcolor=\"#7eff74\"" 
COMMENT             "/ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT             "width 5 offset 0\"") 1 1 1 1 "complement(4224..4241)") 
COMMENT        #6=(CFSignal (CObList) "WPRE" 21 0 0 2846 3433 1 (CStringList) 
COMMENT            (CStringList "/ApEinfo_fwdcolor=\"#7eff74\"" 
COMMENT             "/ApEinfo_revcolor=\"#7eff74\"" 
COMMENT             "/ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT             "width 5 offset 0\"") 1 1 1 1 "3603..4190") 
COMMENT        #7=(CFSignal (CObList) "loxP(3)" 21 0 0 2787 2820 1 (CStringList) 
COMMENT            (CStringList "/ApEinfo_label=\"loxP\"" 
COMMENT             "/ApEinfo_fwdcolor=\"#7eff74\"" "/ApEinfo_revcolor=\"#7eff74\"" 
COMMENT             "/ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT             "width 5 offset 0\"") 1 1 1 1 "3544..3577") 
COMMENT        #8=(CFSignal (CObList) "ORF frame 1" 4 0 0 2760 3485 1 (CStringList) 
COMMENT            (CStringList "/ApEinfo_fwdcolor=\"#e9d024\"" 
COMMENT             "/ApEinfo_revcolor=\"#e9d024\"" 
COMMENT             "/ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT             "width 5 offset 0\"") 1 1 1 1 "3517..4242") 
COMMENT        #9=(CFSignal (CObList) "loxP(2)" 21 0 1 2743 2776 1 (CStringList) 
COMMENT            (CStringList "/ApEinfo_label=\"loxP\"" 
COMMENT             "/ApEinfo_fwdcolor=\"#7eff74\"" "/ApEinfo_revcolor=\"#7eff74\"" 
COMMENT             "/ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT             "width 5 offset 0\"") 1 1 1 1 "complement(3500..3533)") 
COMMENT        #10=(CFSignal (CObList) "loxP(1)" 21 0 0 1983 2016 1 (CStringList) 
COMMENT             (CStringList "/ApEinfo_label=\"loxP\"" 
COMMENT              "/ApEinfo_fwdcolor=\"#7eff74\"" "/ApEinfo_revcolor=\"#7eff74\"" 
COMMENT              "/ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT              "width 5 offset 0\"") 1 1 1 1 "1983..2016") 
COMMENT        #11=(CFSignal (CObList) "loxP" 21 0 0 1939 1972 1 (CStringList) 
COMMENT             (CStringList "/ApEinfo_fwdcolor=\"#7eff74\"" 
COMMENT              "/ApEinfo_revcolor=\"#7eff74\"" 
COMMENT              "/ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT              "width 5 offset 0\"") 1 1 1 1 "1939..1972") 
COMMENT        #12=(CFSignal (CObList) "pCAG_F_primer" 21 0 0 1843 1862 1 (CStringList) 
COMMENT             (CStringList "/ApEinfo_fwdcolor=\"#7eff74\"" 
COMMENT              "/ApEinfo_revcolor=\"#7eff74\"" 
COMMENT              "/ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT              "width 5 offset 0\"") 1 1 1 1 "1843..1862") 
COMMENT        #13=(CFSignal (CObList) "CAG_enhancer" 21 0 0 238 525 1 (CStringList) 
COMMENT             (CStringList "/ApEinfo_fwdcolor=\"#7eff74\"" 
COMMENT              "/ApEinfo_revcolor=\"#7eff74\"" 
COMMENT              "/ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT              "width 5 offset 0\"") 1 1 1 1 "238..525") 
COMMENT        #14=(CFSignal (CObList) "Stop Codon" 21 0 1 2027 2029 1 (CStringList) 
COMMENT             (CStringList "/ApEinfo_fwdcolor=\"#808080\"" 
COMMENT              "/ApEinfo_revcolor=\"green\"" 
COMMENT              "/ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT              "width 5 offset 0\"") 1 1 1 1 "complement(9..11)") 
COMMENT        #15=(CFSignal (CObList) "kozak" 21 0 1 2726 2731 1 (CStringList) 
COMMENT             (CStringList "/ApEinfo_fwdcolor=\"#ff8000\"" 
COMMENT              "/ApEinfo_revcolor=\"green\"" 
COMMENT              "/ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT              "width 5 offset 0\"") 1 1 1 1 "complement(708..713)") 
COMMENT        #16=(CFSignal (CObList) "mScarlet" 21 0 1 2030 2725 1 (CStringList) 
COMMENT             (CStringList "/ApEinfo_fwdcolor=\"#ff0000\"" 
COMMENT              "/ApEinfo_revcolor=\"green\"" 
COMMENT              "/ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT              "width 5 offset 0\"") 1 1 1 1 "complement(12..707)") 
COMMENT        #17=(CFSignal (CObList) "AAV2 ITR" 21 0 0 3703 3843 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #18=(CFSignal (CObList) "AAV2 ITR" 21 0 0 1 141 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #19=(CFSignal (CObList) "Chimeric Intron" 15 0 0 818 1835 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #20=(CFSignal (CObList) "CAG Promoter" 29 0 0 233 816 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #21=(CFSignal (CObList) "AmpR" 21 0 0 4760 5620 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #22=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #23=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) 
COMMENT                    "AAV_pCAG_FLEX_mScarlet" 2) 5 "" 0 4) 
COMMENT              #24=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA 'AAV_pCAG_FLEX_mScarlet'" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Local object" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 04/24/17 04:53PM" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 06/29/17 01:30PM" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6440 bp" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #32=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) (CObjectList)) 
COMMENT                   "Standard Fields") 
COMMENT              #33=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #34=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) 
COMMENT                   "Annotations") 
COMMENT              #35=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 11 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #36=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 0 (0 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #37=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "Result Molecule: AAV_pCAG_FLEX_mScarlet" 1) 
COMMENT                            #38=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "" 1) 
COMMENT                            #39=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "Recipient: AAV_pCAG_FLEX_tdtomato_backbone" 1) 
COMMENT                            #40=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "  Left Terminus: FseI #1 (total 1)" 1) 
COMMENT                            #41=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "  Right Terminus: AscI #1 (total 2)" 1) 
COMMENT                            #42=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "Full digestion" 1) 
COMMENT                            #43=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "" 1) 
COMMENT                            #44=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "Donor: mScarlet_sequence_RCforCloning " 1) 
COMMENT                            #45=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "  Left Terminus: AscI #1 (total 1)" 1) 
COMMENT                            #46=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "    Flank Region: 0" 1) 
COMMENT                            #47=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "  Right Terminus: FseI #1 (total 1)" 1) 
COMMENT                            #48=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "    Flank Region: 0" 1) 
COMMENT                            #49=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "Full digestion" 1) 
COMMENT                            #50=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "" 1) 
COMMENT                            #51=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "Ligation: cohesive termini at both junctions" 
COMMENT                                 1) 
COMMENT                            #52=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "Required orientation achieved" 1) 
COMMENT                            #53=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "" 1) 
COMMENT                            #54=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "Transformation system: Bacteria" 1) 
COMMENT                            #55=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "Extra-chromosomal replication" 1) 
COMMENT                            #56=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "" 1) 
COMMENT                            #57=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "Recommended method(s) for clone analysis:" 1) 
COMMENT                            #58=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "   Restriction Analysis" 1) 
COMMENT                            #59=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "" 1) 
COMMENT                            #60=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "Restriction analysis:" 1) 
COMMENT                            #61=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "   recombinant has new restriction site BclI" 
COMMENT                                 1) 
COMMENT                            #62=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "" 1) 
COMMENT                            #63=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "Recommended oligonucleotide for colony hybridization" 
COMMENT                                 1) 
COMMENT                            #64=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "of cloned fragment in resulting molecule:" 1) 
COMMENT                            #65=(COlyLinePar 
COMMENT                                 (CLinePar 
COMMENT                                  (CParagraph 0 
COMMENT                                   (17 
COMMENT                                    #66=(COligo "" "CGCGCCTGACTTGTACAGCT" "" 0 
COMMENT                                         (CStringList) 0) 0) 1 2 2 0 181) 
COMMENT                                  " CGCGCCTGACTTGTACAGCT" 1)) 
COMMENT                            #67=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "" 1) 
COMMENT                            #68=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "Important Restriction Sites:" 1) 
COMMENT                            #69=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "Cloned fragment may be cut by AscI and FseI" 1) 
COMMENT                            #70=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "Recombinant lacks sites of:" 1) 
COMMENT                            #71=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 " AflII AgeI AsiSI AvrII BaeI BamHI" 1) 
COMMENT                            #72=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 " BbvCI BfrBI BglII BmgBI BmtI Bpu10I" 1) 
COMMENT                            #73=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 " BsaBI BsmI BspEI BstBI BstXI BstZ17I" 1) 
COMMENT                            #74=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 " Bsu36I EcoICRI EcoNI EcoRI EcoRV FspAI" 1) 
COMMENT                            #75=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 " HpaI M.BaeI M.BbvCIA M.BglII M.BnaI M.BstI" 1) 
COMMENT                            #76=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 " M.EcoBI M.EcoR124II M.EcoRI M.EcoRV M.HpaI M.KpnAI" 
COMMENT                                 1) 
COMMENT                            #77=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 " M.KpnBI M.MunI M.PhiBssHII M.PshAI M.SacI M.SsoI" 
COMMENT                                 1) 
COMMENT                            #78=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 " M.Tth111I MfeI MluI NheI NruI NsiI" 1) 
COMMENT                            #79=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 " PacI PmeI Ppu10I PpuMI PshAI PsrI" 1) 
COMMENT                            #80=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 " SacI SanDI SapI SexAI SfiI SpeI" 1) 
COMMENT                            #81=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 " Sse8647I StuI SwaI Tth111I" 1) 
COMMENT                            #82=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "Unique sites inside cloned fragment:" 1) 
COMMENT                            #83=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 " AscI AleI BclI BsrGI BstEII FalI M.BclI M.EcaI" 
COMMENT                                 1) 
COMMENT                            #84=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 " M.XcmI XcmI" 1) 
COMMENT                            #85=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "Unique sites outside cloned fragment:" 1) 
COMMENT                            #86=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 " FseI AarI Acc65I AloI ApaBI BlpI BplI BsaI" 1) 
COMMENT                            #87=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 " BsiWI BspMI BstAPI DraIII FspI HindIII KpnI M.AloI" 
COMMENT                                 1) 
COMMENT                            #88=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 " M.BstVI M.DraIII M.EcoAI M.EcoKI M.HindIII M.KpnI M.NmeSI M.SnaBI" 
COMMENT                                 1) 
COMMENT                            #89=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 " M.StyLTIII M.StySPI M.XbaI NcoI NdeI PciI PflMI PmlI" 
COMMENT                                 1) 
COMMENT                            #90=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 " PsiI PvuI RsrII ScaI SciI SnaBI SphI Sse232I" 
COMMENT                                 1) 
COMMENT                            #91=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 " XbaI XhoI" 1))) "Step #1"))) 
COMMENT                   "Design Description") 
COMMENT              #92=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #93=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #94=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2760 (3 #8# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #95=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2760  End: 3485" 1) 
COMMENT                                   #96=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #97=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3517..4242" 1) 
COMMENT                                   #98=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Qualifiers:" 1) 
COMMENT                                   #99=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   /ApEinfo_fwdcolor=\"#e9d024\"" 1) 
COMMENT                                   #100=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "   /ApEinfo_revcolor=\"#e9d024\"" 1) 
COMMENT                                   #101=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "   /ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT                                         1) 
COMMENT                                   #102=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "   width 5 offset 0\"" 1))) 
COMMENT                                 "ORF frame 1"))) "CDS (1 total)") 
COMMENT                     #103=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 15 (7 15 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #104=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 818 (3 #19# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #105=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 818   End: 1835" 1))) 
COMMENT                                   "Chimeric Intron"))) "Intron (1 total)") 
COMMENT                     #106=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #107=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 1 (3 #18# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #108=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 1     End: 141 " 1))) 
COMMENT                                   "AAV2 ITR") 
COMMENT                             #109=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 238 (3 #13# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #110=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 238   End: 525 " 1) 
COMMENT                                     #111=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #112=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   238..525" 1) 
COMMENT                                     #113=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Qualifiers:" 1) 
COMMENT                                     #114=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_fwdcolor=\"#7eff74\"" 1) 
COMMENT                                     #115=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_revcolor=\"#7eff74\"" 1) 
COMMENT                                     #116=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT                                           1) 
COMMENT                                     #117=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   width 5 offset 0\"" 1))) 
COMMENT                                   "CAG_enhancer") 
COMMENT                             #118=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 1843 (3 #12# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #119=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 1843  End: 1862" 1) 
COMMENT                                     #120=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #121=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   1843..1862" 1) 
COMMENT                                     #122=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Qualifiers:" 1) 
COMMENT                                     #123=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_fwdcolor=\"#7eff74\"" 1) 
COMMENT                                     #124=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_revcolor=\"#7eff74\"" 1) 
COMMENT                                     #125=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT                                           1) 
COMMENT                                     #126=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   width 5 offset 0\"" 1))) 
COMMENT                                   "pCAG_F_primer") 
COMMENT                             #127=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 1939 (3 #11# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #128=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 1939  End: 1972" 1) 
COMMENT                                     #129=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #130=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   1939..1972" 1) 
COMMENT                                     #131=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Qualifiers:" 1) 
COMMENT                                     #132=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_fwdcolor=\"#7eff74\"" 1) 
COMMENT                                     #133=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_revcolor=\"#7eff74\"" 1) 
COMMENT                                     #134=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT                                           1) 
COMMENT                                     #135=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   width 5 offset 0\"" 1))) "loxP") 
COMMENT                             #136=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 1983 (3 #10# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #137=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 1983  End: 2016" 1) 
COMMENT                                     #138=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #139=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   1983..2016" 1) 
COMMENT                                     #140=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Qualifiers:" 1) 
COMMENT                                     #141=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_label=\"loxP\"" 1) 
COMMENT                                     #142=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_fwdcolor=\"#7eff74\"" 1) 
COMMENT                                     #143=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_revcolor=\"#7eff74\"" 1) 
COMMENT                                     #144=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT                                           1) 
COMMENT                                     #145=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   width 5 offset 0\"" 1))) 
COMMENT                                   "loxP(1)") 
COMMENT                             #146=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 2027 (3 #14# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #147=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 2027  End: 2029 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #148=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #149=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(9..11)" 1) 
COMMENT                                     #150=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Qualifiers:" 1) 
COMMENT                                     #151=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_fwdcolor=\"#808080\"" 1) 
COMMENT                                     #152=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_revcolor=\"green\"" 1) 
COMMENT                                     #153=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT                                           1) 
COMMENT                                     #154=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   width 5 offset 0\"" 1))) 
COMMENT                                   "Stop Codon") 
COMMENT                             #155=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 2030 (3 #16# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #156=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 2030  End: 2725 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #157=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #158=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(12..707)" 1) 
COMMENT                                     #159=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Qualifiers:" 1) 
COMMENT                                     #160=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_fwdcolor=\"#ff0000\"" 1) 
COMMENT                                     #161=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_revcolor=\"green\"" 1) 
COMMENT                                     #162=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT                                           1) 
COMMENT                                     #163=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   width 5 offset 0\"" 1))) 
COMMENT                                   "mScarlet") 
COMMENT                             #164=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 2726 (3 #15# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #165=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 2726  End: 2731 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #166=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #167=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(708..713)" 1) 
COMMENT                                     #168=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Qualifiers:" 1) 
COMMENT                                     #169=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_fwdcolor=\"#ff8000\"" 1) 
COMMENT                                     #170=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_revcolor=\"green\"" 1) 
COMMENT                                     #171=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT                                           1) 
COMMENT                                     #172=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   width 5 offset 0\"" 1))) "kozak") 
COMMENT                             #173=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 2743 (3 #9# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #174=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 2743  End: 2776 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #175=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #176=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(3500..3533)" 1) 
COMMENT                                     #177=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Qualifiers:" 1) 
COMMENT                                     #178=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_label=\"loxP\"" 1) 
COMMENT                                     #179=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_fwdcolor=\"#7eff74\"" 1) 
COMMENT                                     #180=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_revcolor=\"#7eff74\"" 1) 
COMMENT                                     #181=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT                                           1) 
COMMENT                                     #182=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   width 5 offset 0\"" 1))) 
COMMENT                                   "loxP(2)") 
COMMENT                             #183=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 2787 (3 #7# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #184=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 2787  End: 2820" 1) 
COMMENT                                     #185=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #186=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   3544..3577" 1) 
COMMENT                                     #187=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Qualifiers:" 1) 
COMMENT                                     #188=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_label=\"loxP\"" 1) 
COMMENT                                     #189=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_fwdcolor=\"#7eff74\"" 1) 
COMMENT                                     #190=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_revcolor=\"#7eff74\"" 1) 
COMMENT                                     #191=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT                                           1) 
COMMENT                                     #192=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   width 5 offset 0\"" 1))) 
COMMENT                                   "loxP(3)") 
COMMENT                             #193=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 2846 (3 #6# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #194=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 2846  End: 3433" 1) 
COMMENT                                     #195=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #196=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   3603..4190" 1) 
COMMENT                                     #197=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Qualifiers:" 1) 
COMMENT                                     #198=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_fwdcolor=\"#7eff74\"" 1) 
COMMENT                                     #199=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_revcolor=\"#7eff74\"" 1) 
COMMENT                                     #200=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT                                           1) 
COMMENT                                     #201=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   width 5 offset 0\"" 1))) "WPRE") 
COMMENT                             #202=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 3467 (3 #5# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #203=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 3467  End: 3484 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #204=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #205=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(4224..4241)" 1) 
COMMENT                                     #206=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Qualifiers:" 1) 
COMMENT                                     #207=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_fwdcolor=\"#7eff74\"" 1) 
COMMENT                                     #208=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_revcolor=\"#7eff74\"" 1) 
COMMENT                                     #209=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT                                           1) 
COMMENT                                     #210=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   width 5 offset 0\"" 1))) 
COMMENT                                   "BGH_rev_primer") 
COMMENT                             #211=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 3703 (3 #17# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #212=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 3703  End: 3843" 1))) 
COMMENT                                   "AAV2 ITR") 
COMMENT                             #213=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 4509 (3 #2# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #214=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 4509  End: 4531" 1) 
COMMENT                                     #215=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #216=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   5266..5288" 1) 
COMMENT                                     #217=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Qualifiers:" 1) 
COMMENT                                     #218=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_fwdcolor=\"#7eff74\"" 1) 
COMMENT                                     #219=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_revcolor=\"#7eff74\"" 1) 
COMMENT                                     #220=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT                                           1) 
COMMENT                                     #221=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   width 5 offset 0\"" 1))) 
COMMENT                                   "pGEX_3_primer") 
COMMENT                             #222=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 4760 (3 #21# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #223=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 4760  End: 5620" 1))) "AmpR"))) 
COMMENT                           "Misc. Feature (15 total)") 
COMMENT                     #224=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #225=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 233 (3 #20# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #226=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 233   End: 816 " 1))) 
COMMENT                                   "CAG Promoter"))) 
COMMENT                           "Promoter Eukaryotic (1 total)") 
COMMENT                     #227=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #228=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 4690 (3 #1# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #229=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 4690  End: 4718" 1) 
COMMENT                                     #230=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #231=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   5447..5475" 1) 
COMMENT                                     #232=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Qualifiers:" 1) 
COMMENT                                     #233=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_fwdcolor=\"#346ee0\"" 1) 
COMMENT                                     #234=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_revcolor=\"#346ee0\"" 1) 
COMMENT                                     #235=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT                                           1) 
COMMENT                                     #236=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   width 5 offset 0\"" 1))) 
COMMENT                                   "AmpR_promoter"))) 
COMMENT                           "Promoter Prokaryotic (1 total)") 
COMMENT                     #237=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #238=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 3935 (3 #3# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #239=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 3935  End: 4241" 1) 
COMMENT                                     #240=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #241=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   4692..4998" 1) 
COMMENT                                     #242=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Qualifiers:" 1) 
COMMENT                                     #243=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_fwdcolor=\"#999999\"" 1) 
COMMENT                                     #244=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_revcolor=\"#999999\"" 1) 
COMMENT                                     #245=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT                                           1) 
COMMENT                                     #246=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   width 5 offset 0\"" 1))) 
COMMENT                                   "f1_origin") 
COMMENT                             #247=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 5775 (3 #0# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #248=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 5775  End: 6394" 1) 
COMMENT                                     #249=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #250=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   6532..7151" 1) 
COMMENT                                     #251=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Qualifiers:" 1) 
COMMENT                                     #252=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_fwdcolor=\"#999999\"" 1) 
COMMENT                                     #253=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_revcolor=\"#999999\"" 1) 
COMMENT                                     #254=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT                                           1) 
COMMENT                                     #255=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   width 5 offset 0\"" 1))) 
COMMENT                                   "pBR322_origin"))) 
COMMENT                           "Replication Origin (2 total)") 
COMMENT                     #256=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #257=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 3470 (3 #4# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #258=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 3470  End: 3673" 1) 
COMMENT                                     #259=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #260=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   4227..4430" 1) 
COMMENT                                     #261=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Qualifiers:" 1) 
COMMENT                                     #262=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_fwdcolor=\"#9d1b1c\"" 1) 
COMMENT                                     #263=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_revcolor=\"#9d1b1c\"" 1) 
COMMENT                                     #264=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT                                           1) 
COMMENT                                     #265=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   width 5 offset 0\"" 1))) 
COMMENT                                   "bGH_PA_terminator"))) "Terminator (1 total)"))) 
COMMENT                   "Feature Map") 
COMMENT              #266=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #267=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 60 (0 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #268=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 4760 (0 0) 1 2 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #269=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Start: 4760  End: 5620" 1) 
COMMENT                                      #270=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Original Location Description:" 1) 
COMMENT                                      #271=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   5517..6377" 1) 
COMMENT                                      #272=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Qualifiers:" 1) 
COMMENT                                      #273=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   /gene=\"Ampicillin\"" 1) 
COMMENT                                      #274=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   /ApEinfo_fwdcolor=\"#ff797d\"" 1) 
COMMENT                                      #275=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   /ApEinfo_revcolor=\"#ff102f\"" 1) 
COMMENT                                      #276=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   /ApEinfo_graphicformat=\"arrow_data {{0 1 2 0 0 -1} {} 0}" 
COMMENT                                            1) 
COMMENT                                      #277=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   width 5 offset 0\"" 1))) 
COMMENT                                    "Ampicillin"))) "Gene (1 total)"))) 
COMMENT                    "Imported Features Not Shown on Map")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #278=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 
COMMENT                "@N (@S)" 0) 
COMMENT          #279=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #280=(CWidgetStyle "Molecule Label 2" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #281=(CWidgetStyle "Molecule Label 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #282=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 
COMMENT                (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #283=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 
COMMENT                (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #284=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 
COMMENT                (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) 
COMMENT          #285=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #286=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #287=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #288=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 128 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #289=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 
COMMENT                1 2 "?" 0) 
COMMENT          #290=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 
COMMENT                0) 
COMMENT          #291=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 
COMMENT                1 65535 "@N (@H)" 0) 
COMMENT          #292=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #293=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT                "Fragment of @N" 0) 
COMMENT          #294=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #295=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT                0 0) 
COMMENT          #296=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT                0) 
COMMENT          #297=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 
COMMENT                (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) 
COMMENT          #298=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #299=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #300=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #301=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #302=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #303=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #304=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #305=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                1 3 "@T @N " 0) 
COMMENT          #306=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #307=(CWidgetStyle "Annotation" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                0 0) 
COMMENT          #308=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #309=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #310=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #311=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 65535 "@N" 0) 
COMMENT          #312=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 
COMMENT                "@N" 0) 
COMMENT          #313=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 3 1 
COMMENT                (LOGBRUSH 0 16777113 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 
COMMENT                (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #314=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 
COMMENT                1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #315=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 
COMMENT                1 1 "@N" 0) 
COMMENT          #316=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #317=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 
COMMENT                "@N (@S)" 0)) 0.164644 1.74233 0.164644 2.53336 
COMMENT         (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 
COMMENT          (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 39 39 0.1 -365) 
COMMENT        1 1 0 1 1 
COMMENT        (mapper: 22.0405 -16.2201 40.0378 40.0378 0.01 10 -1 6440 6440 1 0 0) 
COMMENT        #318=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 745434985 100) 
COMMENT              (CObjectList 
COMMENT               #319=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -1158 100) 
COMMENT                     (CObjectList 
COMMENT                      #320=(CAxis 
COMMENT                            (CWideLine 
COMMENT                             (CWidget 0 (0 0) 1 2 0 0 #284# 92862220 0) 
COMMENT                             (LOGPEN 0 0 10079436) 2 (LOGBRUSH 0 13434879 0) 1 
COMMENT                             6.27516 6.27316 1 0.0214037) 0.0974097) 
COMMENT                      #321=(CLabel (CWidget 1001 (0 0) 1 2 0 0 #281# 513 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 58 22 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 
COMMENT                            1.74233 0.833333 0 "AAV pCAG FLEX mScarlet" "@N" 1 0 
COMMENT                            0.871165 0 -10 14.811 1.39868 Nil) 
COMMENT                      #322=(CLabel (CWidget 1002 (0 0) 1 2 0 0 #280# 0 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 
COMMENT                            1.74233 0.555556 0 "6440 bp" "@L bp" 16 0 -0.871165 
COMMENT                            0 -10.8 2.77238 0.799245 Nil)) (CObjectList)) 
COMMENT               #323=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -1019 100) 
COMMENT                     (CObjectList 
COMMENT                      #324=(CGroupWidget 
COMMENT                            (CWidget 4 (7 4 0) 1 2 0 0 Nil -112 100) 
COMMENT                            (CObjectList 
COMMENT                             #325=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #8# 0) 1 2 0 0 #282# 0 100) 
COMMENT                                    (LOGPEN 0 0 3355545) 1 
COMMENT                                    (LOGBRUSH 0 6724095 0) 1 2.87846 3.58565 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #326=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #279# 753533302 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "ORF frame 1" 
COMMENT                                   "@N" 1 4.88313 -17.4233 0 -10 5.46983 1.04901 
COMMENT                                   #325#)) (CObjectList)) 
COMMENT                      #327=(CGroupWidget 
COMMENT                            (CWidget 15 (7 15 0) 1 2 0 0 Nil -1088 100) 
COMMENT                            (CObjectList 
COMMENT                             #328=(CLine 
COMMENT                                   (CWidget 0 (3 #19# 0) 1 2 0 0 #300# 996 100) 
COMMENT                                   (LOGPEN 0 0 16711680) 1 0.835356 4.48572 
COMMENT                                   5.47735) 
COMMENT                             #329=(CLabel (CWidget 0 (0 0) 1 2 0 0 #279# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Chimeric Intron" "@N" 1 16.4478 3.48466 0 
COMMENT                                   -10 6.71865 1.04901 #328#)) (CObjectList)) 
COMMENT                      #330=(CGroupWidget 
COMMENT                            (CWidget 21 (7 21 0) 1 2 0 0 Nil -740 100) 
COMMENT                            (CObjectList 
COMMENT                             #331=(CScratch 
COMMENT                                   (CWidget 0 (3 #2# 0) 1 2 0 0 #287# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 1.88196 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #332=(CLabel (CWidget 0 (0 0) 1 2 0 0 #279# 2 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "pGEX 3 primer" 
COMMENT                                   "@N" 1 -16.2038 -3.48466 0 -10 6.44391 
COMMENT                                   1.04901 #331#) 
COMMENT                             #333=(CScratch 
COMMENT                                   (CWidget 0 (3 #5# 0) 1 2 0 0 #287# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 2.89696 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #334=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #279# 192 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "BGH rev primer" 
COMMENT                                   "@N" 1 -7.38544 -13.9386 0 -10 6.89349 
COMMENT                                   1.04901 #333#) 
COMMENT                             #335=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #6# 0) 1 2 0 0 #283# 1060 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 2.92911 
COMMENT                                    3.50188 1 0.082322) 0.8 1.8 0) 
COMMENT                             #336=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #279# 2081903016 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "WPRE" "@N" 1 
COMMENT                                   3.35121 -19.1656 0 -10 2.7474 1.04901 #335#) 
COMMENT                             #337=(CScratch 
COMMENT                                   (CWidget 0 (3 #7# 0) 1 2 0 0 #287# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 3.55935 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #338=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #279# 80559688 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "loxP(3)" "@N" 1 
COMMENT                                   7.87573 -15.681 0 -10 3.09707 1.04901 #337#) 
COMMENT                             #339=(CScratch 
COMMENT                                   (CWidget 0 (3 #9# 0) 1 2 0 0 #287# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 3.60221 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #340=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #279# 2081903016 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "loxP(2)" "@N" 1 
COMMENT                                   8.29597 -13.9386 0 -10 3.09707 1.04901 #339#) 
COMMENT                             #341=(CScratch 
COMMENT                                   (CWidget 0 (3 #10# 0) 1 2 0 0 #287# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 4.34252 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #342=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #279# 180 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "loxP(1)" "@N" 1 
COMMENT                                   14.3543 -6.96932 0 -10 3.09707 1.04901 #341#) 
COMMENT                             #343=(CScratch 
COMMENT                                   (CWidget 0 (3 #11# 0) 1 2 0 0 #287# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 4.38539 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #344=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #279# 452 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "loxP" "@N" 1 
COMMENT                                   13.8881 -5.22699 0 -10 2.04806 1.04901 #343#) 
COMMENT                             #345=(CScratch 
COMMENT                                   (CWidget 0 (3 #12# 0) 1 2 0 0 #287# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 4.4789 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #346=(CLabel (CWidget 0 (0 0) 1 2 0 0 #279# 2 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "pCAG F primer" 
COMMENT                                   "@N" 1 16.3848 -3.48466 0 -10 6.44391 1.04901 
COMMENT                                   #345#) 
COMMENT                             #347=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #13# 0) 1 2 0 0 #283# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 5.76178 6.04232 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #348=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #279# 2081903016 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "CAG enhancer" 
COMMENT                                   "@N" 1 9.48782 12.1963 0 -10 6.344 1.04901 
COMMENT                                   #347#) 
COMMENT                             #349=(CScratch 
COMMENT                                   (CWidget 0 (3 #14# 0) 1 2 0 0 #287# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 4.29966 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #350=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #279# 80561648 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "Stop Codon" 
COMMENT                                   "@N" 1 15.3082 -8.71165 0 -10 5.04523 1.04901 
COMMENT                                   #349#) 
COMMENT                             #351=(CScratch 
COMMENT                                   (CWidget 0 (3 #15# 0) 1 2 0 0 #287# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 3.61877 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #352=(CLabel (CWidget 0 (0 0) 1 2 0 0 #279# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "kozak" "@N" 1 
COMMENT                                   8.1335 -12.1963 0 -10 2.6475 1.04901 #351#) 
COMMENT                             #353=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #16# 0) 1 2 0 0 #283# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 3.61877 
COMMENT                                    4.29674 1 0.082322) 0.8 1.8 1) 
COMMENT                             #354=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #279# 753585078 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "mScarlet" "@N" 
COMMENT                                   1 12.2731 -10.454 0 -10 3.97125 1.04901 #353#) 
COMMENT                             #355=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #17# 0) 1 2 0 0 #283# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 2.52973 2.66708 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #356=(CLabel (CWidget 0 (0 0) 1 2 0 0 #279# 1 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "AAV2 ITR" "@N" 
COMMENT                                   1 -11.0394 -10.454 0 -10 4.07115 1.04901 
COMMENT                                   #355#) 
COMMENT                             #357=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #18# 0) 1 2 0 0 #283# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 6.13584 6.27319 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #358=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #279# 452 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "AAV2 ITR" "@N" 
COMMENT                                   1 3.8683 13.9386 0 -10 4.07115 1.04901 #357#) 
COMMENT                             #359=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #21# 0) 1 2 0 0 #283# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 0.79876 1.63746 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #360=(CLabel (CWidget 0 (0 0) 1 2 0 0 #279# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "AmpR" "@N" 1 
COMMENT                                   -14.1388 5.22699 0 -10 2.6475 1.04901 #359#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #361=(CGroupWidget 
COMMENT                            (CWidget 29 (7 29 0) 1 2 0 0 Nil -1088 100) 
COMMENT                            (CObjectList 
COMMENT                             #362=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #20# 0) 1 2 0 0 #283# 904 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 5.47832 
COMMENT                                    6.04719 1 0.082322) 0.8 1.8 0) 
COMMENT                             #363=(CLabel (CWidget 0 (0 0) 1 2 0 0 #279# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "CAG Promoter" 
COMMENT                                   "@N" 1 12.1458 10.454 0 -10 6.26908 1.04901 
COMMENT                                   #362#)) (CObjectList)) 
COMMENT                      #364=(CGroupWidget 
COMMENT                            (CWidget 30 (7 30 0) 1 2 0 0 Nil -182 100) 
COMMENT                            (CObjectList 
COMMENT                             #365=(CPromoterArrow 
COMMENT                                   (CLine 
COMMENT                                    (CWidget 0 (3 #1# 0) 1 2 0 0 #301# 0 100) 
COMMENT                                    (LOGPEN 0 4 128) 6 1.16464 1.61838 1.70564) 
COMMENT                                   0.4 0.082322 1.08464 1.8 1.8 0) 
COMMENT                             #366=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #279# 754558202 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "AmpR promoter" 
COMMENT                                   "@N" 1 -16.8888 -1.74233 0 -10 6.79358 
COMMENT                                   1.04901 #365#)) (CObjectList)) 
COMMENT                      #367=(CGroupWidget 
COMMENT                            (CWidget 33 (7 33 0) 1 2 0 0 Nil 1629633908 100) 
COMMENT                            (CObjectList 
COMMENT                             #368=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #0# 0) 1 2 0 0 #282# 0 100) 
COMMENT                                    (LOGPEN 0 0 3355545) 1 
COMMENT                                    (LOGBRUSH 0 6724095 0) 1 0.0448085 0.648749 
COMMENT                                    1 0.082322) 0.8 1.8 0) 
COMMENT                             #369=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #279# 538 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "pBR322 origin" 
COMMENT                                   "@N" 1 -9.42684 12.1963 0 -10 6.16917 1.04901 
COMMENT                                   #368#) 
COMMENT                             #370=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #3# 0) 1 2 0 0 #282# 0 100) 
COMMENT                                    (LOGPEN 0 0 3355545) 1 
COMMENT                                    (LOGBRUSH 0 6724095 0) 1 2.14204 2.44109 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #371=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #279# 522 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "f1 origin" "@N" 
COMMENT                                   1 -12.5063 -8.71165 0 -10 3.62158 1.04901 
COMMENT                                   #370#)) (CObjectList)) 
COMMENT                      #372=(CGroupWidget 
COMMENT                            (CWidget 43 (7 43 0) 1 2 0 0 Nil -251 100) 
COMMENT                            (CObjectList 
COMMENT                             #373=(CLine 
COMMENT                                   (CWidget 0 (3 #4# 0) 1 2 0 0 #287# 596 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 0.835356 2.69533 
COMMENT                                   2.89404) 
COMMENT                             #374=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #279# 2081903016 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "bGH PA terminator" "@N" 1 -10.4113 -12.1963 
COMMENT                                   0 -10 8.11733 1.04901 #373#)) (CObjectList))) 
COMMENT                     (CObjectList)) 
COMMENT               #375=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -1158 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #376=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -1019 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #377=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -182 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 277 
COMMENT       (CStringList) 216 1 (CObList)))
FEATURES             Location/Qualifiers
     rep_origin      5775..6394
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="33"
                     /label=pBR322_origin
     gene            4760..5620
                     /gene="Ampicillin"
                     /ApEinfo_fwdcolor="#ff797d"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="60"
     promoter        4690..4718
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="30"
                     /label=AmpR_promoter
     misc_feature    4509..4531
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=pGEX_3_primer
     rep_origin      3935..4241
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="33"
                     /label=f1_origin
     terminator      3470..3673
                     /ApEinfo_fwdcolor="#9d1b1c"
                     /ApEinfo_revcolor="#9d1b1c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="43"
                     /label=bGH_PA_terminator
     misc_feature    complement(3467..3484)
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=BGH_rev_primer
     misc_feature    2846..3433
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=WPRE
     misc_feature    2787..2820
                     /ApEinfo_label="loxP"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=loxP(3)
     CDS             2760..3485
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="4"
                     /label=ORF\frame\1
     misc_feature    complement(2743..2776)
                     /ApEinfo_label="loxP"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=loxP(2)
     misc_feature    1983..2016
                     /ApEinfo_label="loxP"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=loxP(1)
     misc_feature    1939..1972
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=loxP
     misc_feature    1843..1862
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=pCAG_F_primer
     misc_feature    238..525
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=CAG_enhancer
     misc_feature    complement(2027..2029)
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=Stop\Codon
     misc_feature    complement(2726..2731)
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=kozak
     misc_feature    complement(2030..2725)
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /vntifkey="21"
                     /label=mScarlet
     misc_feature    3703..3843
                     /vntifkey="21"
                     /label=AAV2\ITR
     misc_feature    1..141
                     /vntifkey="21"
                     /label=AAV2\ITR
     intron          818..1835
                     /vntifkey="15"
                     /label=Chimeric\Intron
     promoter        233..816
                     /vntifkey="29"
                     /label=CAG\Promoter
     misc_feature    4760..5620
                     /vntifkey="21"
                     /label=AmpR
BASE COUNT     1218 a      1785 c      1894 g      1543 t 
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 
      121 actccatcac taggggttcc tgcggccgca cgcgaaacaa ttctgcagga atctagttat 
      181 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca 
      241 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca 
      301 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg 
      361 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg 
      421 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc 
      481 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc 
      541 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt 
      601 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg 
      661 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg 
      721 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg 
      781 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggga gtcgctgcga cgctgccttc 
      841 gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt 
      901 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg 
      961 tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg 
     1021 gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg 
     1081 ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt 
     1141 gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg 
     1201 ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg 
     1261 gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga gttgctgagc 
     1321 acggcccggc ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg 
     1381 gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg 
     1441 gctcggggga ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc 
     1501 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa 
     1561 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga 
     1621 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc 
     1681 gccgtcccct tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg 
     1741 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg 
     1801 ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg 
     1861 tgctgtctca tcattttggc aaagaattga tttgataccg cgggccccgc gtggacggta 
     1921 cccttcaggt cgacgtcgat aacttcgtat agcatacatt atacgaagtt atccgaatcg 
     1981 caataacttc gtataggata ctttatacga agttatttgg cgcgcctgac ttgtacagct 
     2041 cgtccatgcc gccggtggag tggcggccct cggagcgttc gtactgttcc accacggtgt 
     2101 agtcctcgtt gtgggaggtg atgtccaact tgcggtcgac gttgtaggcg ccgggcatct 
     2161 gcacgggctt cttggccttg taggtggtct tgaagtccgc caggtagcgg ccgccgtcct 
     2221 tcaggcgcag ggccatctta atgtcgccct tcagcacgcc gtcctcgggg tacaaccgct 
     2281 cggtggacgc ttcccagccc attgtcttct tctgcattac ggggccgtca ggagggaagt 
     2341 tggtgccgcg gagcttcacc ttgtagatca gggtgccgtc ctccagggag gtgtcctggg 
     2401 tcacggtcac ggcgccgccg tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg 
     2461 ggaaggactg cttatagtag tcggggatgt cggcggggtg cttggtgaag gccctggagc 
     2521 cgtacatgaa ctgaggggac aggatgtccc aggagaaggg cagggggcca cccttggtca 
     2581 ccttcagctt ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct 
     2641 cgaactcgtg gccgttcatg gagccctcca tgtgcacctt gaaccgcatg aactccttga 
     2701 tcactgcctc gcccttgctc accatgccac cggccggcct tgataacttc gtataatgta 
     2761 tgctatacga agttatccga atcgcaataa cttcgtataa agtatcctat acgaagttat 
     2821 cgaagatcat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga 
     2881 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 
     2941 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 
     3001 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 
     3061 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 
     3121 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 
     3181 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat 
     3241 catcgtcctt tccttggctg ctcgcctatg ttgccacctg gattctgcgc gggacgtcct 
     3301 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 
     3361 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg 
     3421 ccgcctcccc gcatcgatac cgagcgctgc tcgagagatc gatctgcctc gactgtgcct 
     3481 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt 
     3541 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg 
     3601 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac 
     3661 aatagcaggc atgctgggga cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg 
     3721 agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg 
     3781 cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc 
     3841 aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac 
     3901 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt 
     3961 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt 
     4021 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc 
     4081 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga 
     4141 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc 
     4201 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg 
     4261 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct 
     4321 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg 
     4381 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac 
     4441 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt 
     4501 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag 
     4561 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc 
     4621 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 
     4681 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 
     4741 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt 
     4801 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc 
     4861 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat 
     4921 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct 
     4981 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca 
     5041 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg 
     5101 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa 
     5161 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg 
     5221 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga 
     5281 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg 
     5341 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt 
     5401 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg 
     5461 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc 
     5521 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca 
     5581 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc 
     5641 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat 
     5701 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc 
     5761 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 
     5821 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 
     5881 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct 
     5941 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 
     6001 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 
     6061 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 
     6121 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 
     6181 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 
     6241 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 
     6301 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 
     6361 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 
     6421 ctggcctttt gctcacatgt 
//
