LOCUS       Exported                9132 bp ds-DNA    circular SYN 31-MAY-2014
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pCW57.1_Dux4_Codon_altered_V2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jun 29, 2017 from SnapGene Viewer 2.4.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9132
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    41..158
                     /note="cPPT/CTS"
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /note="tight TRE promoter"
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             585..1859
                     /codon_start=1
                     /note="Dux4_CA"
                     /translation="MALPTPSDSTLPAEARGRGRRRRLVWTPSQSEALRACFERNPYPG
                     IATRERLAQAIGIPEPRVQIWFQNERSRQLRQHRRESRPWPGRRGPPEGRRKRTAVTGS
                     QTALLLRAFEKDRFPGIAAREELARETGLPESRIQIWFQNRRARHPGQGGRAPAQAGGL
                     CSAAPGGGHPAPSWVAFAHTGAWGTGLPAPHVPCAPGALPQGAFVSQAARAAPALQPSQ
                     AAPAEGISQPAPARGDFAYAAPAPPDGALSHPQAPRWPPHPGKSREDRDPQRDGLPGPC
                     AVAQPGPAQAGPQGQGVLAPPTSQGSPWWGWGRGPQVAGAAWEPQAGAAPPPQPAPPDA
                     SASARQGQMQGIPAPSQALQEPAPWSALPCGLLLDELLASPEFLQQAQPLLETEAPGEL
                     EASEEAASLEAPLSEEEYRALLEEL"
     primer_bind     complement(1886..1902)
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1905..2029)
                     /gene="mutant version of attR"
                     /bound_moiety="LR Clonase(TM)"
                     /note="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2047..2064
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     promoter        2074..2584
                     /note="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             2594..3190
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     CDS             3191..3244
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /note="T2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3245..3991
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /note="rtTA-Advanced"
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(4449..4460)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /translation="IEGR"
     LTR             4665..4898
                     /note="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4970..5091
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      5131..5266
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     CDS             5338..6198
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6369..6957
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    7245..7266
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7281..7311
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    7319..7335
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7343..7359
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7380..7398
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7424..7652
                     /note="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7653..7833
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7880..8005
                     /note="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8498..8731
                     /note="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gaactagtgg atcccccggg ctgcaggaat tcgcccttgc caccatggca ttgcctacac
      601 cttcagactc tacgctgcct gcagaggcta ggggaagagg tagacggcgg cgattggtgt
      661 ggactccatc acaatccgaa gctcttcgcg catgcttcga gcgcaatccc tatccgggga
      721 ttgccacaag ggagaggctt gcacaggcta tcggaatccc ggaaccgaga gtgcagatct
      781 ggttccaaaa tgaacgctct cggcagctca gacagcatcg cagggagtcc cgcccgtggc
      841 caggaagaag gggaccacct gaaggaagaa gaaaacgcac agcggtgact ggcagccaaa
      901 cggctctgct gctccgcgct ttcgagaaag atcggttccc cggaattgcc gcacgcgaag
      961 aactcgccag agaaactggg ctcccagaat cacgaataca gatttggttc cagaaccgca
     1021 gagcaagaca cccaggccag gggggacggg cacctgctca ggccggtgga ctctgctctg
     1081 ctgcccctgg gggcggccat ccagcacctt cctgggtggc tttcgctcat actggcgctt
     1141 ggggtaccgg gctgcctgct ccgcatgttc cctgtgctcc aggggccctc ccgcagggag
     1201 cgtttgtttc ccaggcagct agggctgcac ctgccctgca accatcacag gcagcgccag
     1261 ctgaaggcat cagccaaccc gccccagccc gcggagattt tgcttatgca gcgccagcac
     1321 ctccagacgg tgccctgagc cacccccaag cccccagatg gccccctcac cctggtaagt
     1381 cccgggaaga ccgcgatccc caacgagatg gactgcccgg tccttgcgct gtggcccagc
     1441 caggacctgc tcaagccggc cctcaggggc aaggagtgct ggccccacct acaagccagg
     1501 gatctccctg gtggggttgg ggacgcggac ctcaggttgc tggagccgct tgggagcctc
     1561 aggccggagc tgcaccgccg ccacaaccgg cccctcccga cgcgtcagcg tccgcccgac
     1621 aaggccagat gcagggaatc ccagcaccta gccaagctct tcaagagcct gccccttgga
     1681 gcgcactgcc gtgtgggctg ctcctggatg aactcctggc tagcccagaa tttctccagc
     1741 aggcacagcc actcctggaa acagaagctc cgggagagct cgaagcctcc gaagaagcag
     1801 caagcctgga ggcacctctt tccgaggagg agtatagagc ccttctggaa gaactttgaa
     1861 agggcgaatt cgatatcaag cttatcgata ccgtcgacct cgaccatagt gactggatat
     1921 gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga
     1981 tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt tagtaatgaa
     2041 ccggtccacc accaccacca ccactaagga tccggggttg gggttgcgcc ttttccaagg
     2101 cagccctggg tttgcgcagg gacgcggctg ctctgggcgt ggttccggga aacgcagcgg
     2161 cgccgaccct gggtctcgca cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc
     2221 cgctaccctt gtgggccccc cggcgacgct tcctgctccg cccctaagtc gggaaggttc
     2281 cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca ctagtaccct
     2341 cgcagacgga cagcgccagg gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa
     2401 tagcggctgc tcagcagggc gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc
     2461 ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg cattctgcaa
     2521 gcctccggag cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg acctctctcc
     2581 ccagcaattc accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     2641 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     2701 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     2761 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     2821 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     2881 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     2941 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     3001 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     3061 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     3121 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     3181 gcccggtgcc gaaggtagag gttctctcct cacttgtggt gatgttgaag aaaaccctgg
     3241 tccaatgtct agactggaca agagcaaagt cataaacgga gctctggaat tactcaatgg
     3301 tgtcggtatc gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc
     3361 taccctgtac tggcacgtga agaacaagcg ggccctgctc gatgccctgc caatcgagat
     3421 gctggacagg catcataccc acttctgccc cctggaaggc gagtcatggc aagactttct
     3481 gcggaacaac gccaagtcat accgctgtgc tctcctctca catcgcgacg gggctaaagt
     3541 gcatctcggc acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt
     3601 cctgtgtcag caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca
     3661 ctttacactg ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga
     3721 gacacctacc accgattcta tgcccccact tctgagacaa gcaattgagc tgttcgaccg
     3781 gcagggagcc gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa
     3841 acagctaaag tgcgaaagcg gcgggccgac cgacgccctt gacgattttg acttagacat
     3901 gctcccagcc gatgcccttg acgactttga ccttgatatg ctgcctgctg acgctcttga
     3961 cgattttgac cttgacatgc tccccgggta actaagtaag gatcgatcca agatatcgta
     4021 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     4081 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     4141 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     4201 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     4261 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     4321 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     4381 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     4441 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     4501 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     4561 ccccgcctgt ttcgcctcgg cgtccggact agaggtacct ttaagaccaa tgacttacaa
     4621 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     4681 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     4741 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     4801 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     4861 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     4921 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     4981 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     5041 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     5101 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     5161 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     5221 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctttc aaatatgtat
     5281 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     5341 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     5401 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     5461 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     5521 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     5581 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     5641 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     5701 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     5761 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     5821 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     5881 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     5941 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     6001 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     6061 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     6121 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     6181 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     6241 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     6301 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     6361 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     6421 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     6481 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     6541 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     6601 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     6661 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     6721 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     6781 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     6841 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     6901 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     6961 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     7021 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     7081 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     7141 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     7201 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     7261 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     7321 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
     7381 attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa
     7441 tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga
     7501 aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa
     7561 ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata
     7621 ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag
     7681 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     7741 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     7801 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     7861 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     7921 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     7981 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     8041 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     8101 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     8161 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     8221 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     8281 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     8341 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     8401 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     8461 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     8521 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     8581 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     8641 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     8701 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     8761 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     8821 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     8881 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     8941 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     9001 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     9061 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     9121 ccccgagggg ac
//
