LOCUS Exported 9462 bp ds-DNA circular SYN 31-MAY-2014 DEFINITION . ACCESSION . VERSION . KEYWORDS pCW57.1_luciferase SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9462) AUTHORS . TITLE Direct Submission JOURNAL Exported Thursday, Jun 29, 2017 from SnapGene Viewer 2.4.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9462 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 41..158 /note="cPPT/CTS" /note="central polypurine tract and central termination sequence of HIV-1" promoter 212..526 /note="tight TRE promoter" /note="Tet-responsive promoter PTight, consisting of seven tet operator sequences followed by the minimal CMV promoter" protein_bind 220..238 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 256..274 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 291..309 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 327..345 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 363..381 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 398..416 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 434..452 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" CDS 569..2221 /codon_start=1 /gene="luc+" /product="firefly luciferase" /note="luciferase" /note="enhanced luc+ version of the luciferase gene" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV" protein_bind complement(2235..2359) /gene="mutant version of attR" /bound_moiety="LR Clonase(TM)" /note="attR2" /note="recombination site for the Gateway(R) LR reaction" CDS 2377..2394 /codon_start=1 /product="6xHis affinity tag" /note="6xHis" /translation="HHHHHH" promoter 2404..2914 /note="hPGK promoter" /note="human phosphoglycerate kinase 1 promoter" CDS 2924..3520 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /note="PuroR" /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" CDS 3521..3574 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /note="T2A" /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 3575..4321 /codon_start=1 /product="improved tetracycline-controlled transactivator" /note="rtTA-Advanced" /note="In the Tet-On(R) system, rtTA-Advanced binds to the Tet-responsive element and stimulates transcription only in the presence of tetracycline or doxycycline." /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA DALDDFDLDMLPADALDDFDLDMLPG" CDS complement(4779..4790) /codon_start=1 /product="Factor Xa recognition and cleavage site" /note="Factor Xa site" /translation="IEGR" LTR 4995..5228 /note="3' LTR (Delta-U3)" /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 5300..5421 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" rep_origin 5461..5596 /note="SV40 ori" /note="SV40 origin of replication" CDS 5668..6528 /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 6699..7287 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 7575..7596 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." promoter 7611..7641 /note="lac promoter" /note="promoter for the E. coli lac operon" protein_bind 7649..7665 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 7673..7689 /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" promoter 7710..7728 /note="T3 promoter" /note="promoter for bacteriophage T3 RNA polymerase" promoter 7754..7982 /note="RSV promoter" /note="Rous sarcoma virus enhancer/promoter" LTR 7983..8163 /note="5' LTR (truncated)" /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 8210..8335 /note="HIV-1 Psi" /note="packaging signal of human immunodeficiency virus type 1" misc_feature 8828..9061 /note="RRE" /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." ORIGIN 1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga 61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta 121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca 181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac 241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag 301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt 361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt 421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg 481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta 541 gcattccggt actgttggta aagccaccat ggaagacgcc aaaaacataa agaaaggccc 601 ggcgccattc tatccgctgg aagatggaac cgctggagag caactgcata aggctatgaa 661 gagatacgcc ctggttcctg gaacaattgc ttttacagat gcacatatcg aggtggacat 721 cacttacgct gagtacttcg aaatgtccgt tcggttggca gaagctatga aacgatatgg 781 gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc 841 ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca tttataatga 901 acgtgaattg ctcaacagta tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa 961 ggggttgcaa aaaattttga acgtgcaaaa aaagctccca atcatccaaa aaattattat 1021 catggattct aaaacggatt accagggatt tcagtcgatg tacacgttcg tcacatctca 1081 tctacctccc ggttttaatg aatacgattt tgtgccagag tccttcgata gggacaagac 1141 aattgcactg atcatgaact cctctggatc tactggtctg cctaaaggtg tcgctctgcc 1201 tcatagaact gcctgcgtga gattctcgca tgccagagat cctatttttg gcaatcaaat 1261 cattccggat actgcgattt taagtgttgt tccattccat cacggttttg gaatgtttac 1321 tacactcgga tatttgatat gtggatttcg agtcgtctta atgtatagat ttgaagaaga 1381 gctgtttctg aggagccttc aggattacaa gattcaaagt gcgctgctgg tgccaaccct 1441 attctccttc ttcgccaaaa gcactctgat tgacaaatac gatttatcta atttacacga 1501 aattgcttct ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt 1561 ccatctgcca ggtatcaggc aaggatatgg gctcactgag actacatcag ctattctgat 1621 tacacccgag ggggatgata aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc 1681 gaaggttgtg gatctggata ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg 1741 tgtgagaggt cctatgatta tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt 1801 gattgacaag gatggatggc tacattctgg agacatagct tactgggacg aagacgaaca 1861 cttcttcatc gttgaccgcc tgaagtctct gattaagtac aaaggctatc aggtggctcc 1921 cgctgaattg gaatccatct tgctccaaca ccccaacatc ttcgacgcag gtgtcgcagg 1981 tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa 2041 gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa 2101 gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga 2161 cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaaga tcgccgtgta 2221 attctagact cgaccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt 2281 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag 2341 ctttcttgta caaagtggtt tagtaatgaa ccggtccacc accaccacca ccactaagga 2401 tccggggttg gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg 2461 ctctgggcgt ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac 2521 gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct 2581 tcctgctccg cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca 2641 aacggaagcc gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc 2701 agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag 2761 cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt 2821 cctgcccgcg cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc 2881 cctcgttgac cgaatcaccg acctctctcc ccagcaattc accatgaccg agtacaagcc 2941 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc 3001 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt 3061 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt 3121 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc 3181 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca 3241 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc 3301 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc 3361 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg 3421 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga 3481 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc gaaggtagag gttctctcct 3541 cacttgtggt gatgttgaag aaaaccctgg tccaatgtct agactggaca agagcaaagt 3601 cataaacgga gctctggaat tactcaatgg tgtcggtatc gaaggcctga cgacaaggaa 3661 actcgctcaa aagctgggag ttgagcagcc taccctgtac tggcacgtga agaacaagcg 3721 ggccctgctc gatgccctgc caatcgagat gctggacagg catcataccc acttctgccc 3781 cctggaaggc gagtcatggc aagactttct gcggaacaac gccaagtcat accgctgtgc 3841 tctcctctca catcgcgacg gggctaaagt gcatctcggc acccgcccaa cagagaaaca 3901 gtacgaaacc ctggaaaatc agctcgcgtt cctgtgtcag caaggcttct ccctggagaa 3961 cgcactgtac gctctgtccg ccgtgggcca ctttacactg ggctgcgtat tggaggaaca 4021 ggagcatcaa gtagcaaaag aggaaagaga gacacctacc accgattcta tgcccccact 4081 tctgagacaa gcaattgagc tgttcgaccg gcagggagcc gaacctgcct tccttttcgg 4141 cctggaacta atcatatgtg gcctggagaa acagctaaag tgcgaaagcg gcgggccgac 4201 cgacgccctt gacgattttg acttagacat gctcccagcc gatgcccttg acgactttga 4261 ccttgatatg ctgcctgctg acgctcttga cgattttgac cttgacatgc tccccgggta 4321 actaagtaag gatcgatcca agatatcgta ttcttaacta tgttgctcct tttacgctat 4381 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt 4441 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca 4501 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg 4561 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg 4621 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca 4681 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca 4741 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc 4801 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc 4861 agacgagtcg gatctccctt tgggccgcct ccccgcctgt ttcgcctcgg cgtccggact 4921 agaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa 4981 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc 5041 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 5101 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 5161 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 5221 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg 5281 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa 5341 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 5401 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc 5461 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 5521 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 5581 ggcttttttg gaggcctttc aaatatgtat ccgctcatga gacaataacc ctgataaatg 5641 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt 5701 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta 5761 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc 5821 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa 5881 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc 5941 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt 6001 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact 6061 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac 6121 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata 6181 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta 6241 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg 6301 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat 6361 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt 6421 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga 6481 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa 6541 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag 6601 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 6661 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 6721 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 6781 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 6841 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 6901 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 6961 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 7021 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 7081 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 7141 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 7201 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 7261 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 7321 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 7381 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc 7441 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg 7501 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt 7561 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt 7621 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac 7681 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg 7741 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga 7801 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag 7861 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac 7921 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa 7981 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc 8041 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt 8101 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta 8161 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg 8221 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac 8281 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat 8341 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa 8401 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa 8461 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc 8521 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg 8581 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga 8641 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag 8701 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac 8761 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag 8821 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag 8881 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga 8941 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca 9001 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc 9061 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta 9121 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca 9181 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc 9241 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt 9301 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg 9361 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt 9421 caccattatc gtttcagacc cacctcccaa ccccgagggg ac //