LOCUS Exported 9810 bp ds-DNA circular UNA 29-JUN-2017 DEFINITION EcoRV.BstBI.NheI.kzk.mDUX_opti.SalI.EcoRV_FINAL ORDER (bases 14 to 2057) inserted into pCW57.1 (bases 2122 to 541). ACCESSION . VERSION . KEYWORDS pCW57.1_mDUX_opti_(CA) SOURCE natural DNA sequence ORGANISM unspecified REFERENCE 1 (bases 1 to 9810) AUTHORS . TITLE Direct Submission JOURNAL Exported Thursday, Jun 29, 2017 from SnapGene Viewer 2.4.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9810 /organism="unspecified" /mol_type="genomic DNA" misc_feature 41..158 /note="cPPT/CTS" /note="central polypurine tract and central termination sequence of HIV-1" promoter 212..526 /note="tight TRE promoter" /note="Tet-responsive promoter PTight, consisting of seven tet operator sequences followed by the minimal CMV promoter" protein_bind 220..238 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 256..274 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 291..309 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 327..345 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 363..381 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 398..416 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 434..452 /gene="tetO" /bound_moiety="tetracycline repressor TetR" /note="tet operator" /note="bacterial operator O2 for the tetR and tetA genes" CDS 552..2576 /codon_start=1 /note="mDUX-CA" /translation="MAEAGSPVGGSGVARESRRRRKTVWQAWQEQALLSTFKKKRYLSF KERKELAKRMGVSDCRIRVWFQNRRNRSGEEGHASKRSIRGSRRLASPQLQEELGSRPQ GRGMRSSGRRPRTRLTSLQLRILGQAFERNPRPGFATREELARDTGLPEDTIHIWFQNR RARRRHRRGRPTAQDQDLLASQGSDGAPAGPEGREREGAQENLLPQEEAGSTGMDTSSP SDLPSFCGESQPFQVAQPRGAGQQEAPTRAGNAGSLEPLLDQLLDEVQVEEPAPAPLNL DGDPGGRVHEGSQESFWPQEEAGSTGMDTSSPSDSNSFCRESQPSQVAQPCGAGQEDAR TQADSTGPLELLLLDQLLDEVQKEEHVPVPLDWGRNPGSREHEGSQDSLLPLEEAVNSG MDTSIPSIWPTFCRESQPPQVAQPSGPGQAQAPTQGGNTDPLELFLYQLLDEVQVEEHA PAPLNWDVDPGGRVHEGSWESFWPQEEAGSTGLDTSSPSDSNSFFRESKPSQVAQRRGA GQEDARTQADSTGPLELLLFDQLLDEVQKEEHVPAPLDWGRNPGSMEHEGSQDSLLPLE EAANSGRDTSIPSIWPAFCRKSQPPQVAQPSGPGQAQAPIQGGNTDPLELFLDQLLTEV QLEEQGPAPVNVEETWEQMDTTPDLPLTSEEYQTLLDML" misc_feature 2578..2582 /note="Geneious name: Typical insertion site (removes ccdB)" protein_bind complement(2583..2707) /gene="mutant version of attR" /bound_moiety="LR Clonase(TM)" /note="attR2" /note="recombination site for the Gateway(R) LR reaction" CDS 2725..2742 /codon_start=1 /product="6xHis affinity tag" /note="6xHis" /translation="HHHHHH" promoter 2752..3262 /note="hPGK promoter" /note="human phosphoglycerate kinase 1 promoter" CDS 3272..3868 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /note="PuroR" /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" CDS 3869..3922 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /note="T2A" /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 3923..4669 /codon_start=1 /product="improved tetracycline-controlled transactivator" /note="rtTA-Advanced" /note="In the Tet-On(R) system, rtTA-Advanced binds to the Tet-responsive element and stimulates transcription only in the presence of tetracycline or doxycycline." /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA DALDDFDLDMLPADALDDFDLDMLPG" CDS complement(5127..5138) /codon_start=1 /product="Factor Xa recognition and cleavage site" /note="Factor Xa site" /translation="IEGR" LTR 5343..5576 /note="3' LTR (Delta-U3)" /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 5648..5769 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" rep_origin 5809..5944 /note="SV40 ori" /note="SV40 origin of replication" promoter 5944..6015 /gene="bla" /note="AmpR promoter" CDS 6016..6876 /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 7047..7635 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 7923..7944 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." promoter 7959..7989 /note="lac promoter" /note="promoter for the E. coli lac operon" protein_bind 7997..8013 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 8021..8037 /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" promoter 8058..8076 /note="T3 promoter" /note="promoter for bacteriophage T3 RNA polymerase" promoter 8102..8330 /note="RSV promoter" /note="Rous sarcoma virus enhancer/promoter" LTR 8331..8511 /note="5' LTR (truncated)" /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 8558..8683 /note="HIV-1 Psi" /note="packaging signal of human immunodeficiency virus type 1" misc_feature 9176..9409 /note="RRE" /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." ORIGIN 1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga 61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta 121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca 181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac 241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag 301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt 361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt 421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg 481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta 541 gcgccgccac catggctgag gctggctctc cagtgggagg atctggagtg gccagagaat 601 caaggagaag gaggaaaact gtctggcaag cttggcagga acaggcactc ctgagcacat 661 ttaagaaaaa aaggtatctg tcctttaaag aaagaaagga actggcaaaa aggatgggag 721 tttctgattg caggatcaga gtctggttcc agaataggag aaataggtct ggggaggaag 781 gacatgcaag caagagaagc ataagaggtt ccaggaggct ggcatcccct caacttcagg 841 aggaactggg aagtaggccc caaggcaggg gcatgaggtc ctcagggagg agacccagaa 901 ccaggctgac aagtctgcag ctgagaatcc ttggtcaggc ttttgaaagg aatccaaggc 961 caggatttgc caccagagag gaactggcca gggatacagg ccttcctgag gatactatcc 1021 atatctggtt ccagaacagg agggccagga gaaggcacag aaggggaaga cctacagccc 1081 aggaccagga cctcctggct tcccagggtt ctgatggagc acctgctggg cctgaaggta 1141 gagagagaga aggagcacag gaaaatttgc tgccccagga ggaggcagga tcaacaggga 1201 tggacacctc aagcccttct gacctccctt cattctgtgg tgaatcacag ccctttcagg 1261 tggcccagcc caggggagct ggacagcagg aggctcccac aagggcaggg aatgctggat 1321 cattggagcc actgttggac cagctcttgg atgaggtcca ggtggaggaa cctgccccag 1381 ctccactcaa cctggatggt gatcctgggg ggagggttca tgagggtagt caggagtcct 1441 tctggcccca ggaggaggct ggttctactg gaatggacac ttcttcaccc tctgacagca 1501 atagcttttg cagggagagt caaccctctc aggtagctca gccttgtggg gctggccagg 1561 aggatgctag gacccaggct gactcaacag ggcccttgga gctgttgctg ctggaccagc 1621 tcctggatga ggtacagaag gaggaacatg taccagtgcc cctggactgg gggaggaacc 1681 ctggaagcag agaacatgag ggtagtcagg attctctcct tcctctggaa gaggctgtga 1741 attctggaat ggacactagt ataccaagta tttggcctac attttgcagg gagtcacaac 1801 ccccacaggt ggctcagcct tcaggacctg ggcaggccca ggctcctacc caagggggta 1861 atacagaccc actggaactc tttctgtatc agctgctgga tgaggtccag gtggaggaac 1921 atgccccagc tccactcaac tgggatgtgg atccaggggg cagagtccat gagggttcct 1981 gggagtcatt ctggccccag gaggaggcag gctctacagg actggacaca agctccccta 2041 gtgacagcaa ctcattcttt agggagagta agccctctca ggttgctcaa aggaggggag 2101 ctgggcaaga ggatgccagg actcaggctg acagtacagg acccctggag ctgctgttgt 2161 ttgaccagct cctggatgaa gtgcagaagg aggaacatgt tccagctccc ctggactggg 2221 gaaggaaccc tggttctatg gaacatgagg gctctcagga ctctctcttg cctctggaag 2281 aagctgctaa tagtggcaga gatacaagta tcccaagcat ttggcctgcc ttttgcagga 2341 aaagccagcc accccaggta gcccagccta gtggacctgg acaggctcag gcacctatac 2401 aaggaggcaa cactgaccca ttggagttgt ttctggacca gctgctcact gaggtgcaac 2461 tggaggaaca agggccagca cctgtcaatg ttgaagagac ctgggaacag atggatacca 2521 ctccagactt gccactgact tctgaagagt accagaccct tcttgacatg ctgtaagtcg 2581 accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa 2641 tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca 2701 aagtggttta gtaatgaacc ggtccaccac caccaccacc actaaggatc cggggttggg 2761 gttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg 2821 ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag 2881 cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc ctgctccgcc 2941 cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc 3001 acgtctcact agtaccctcg cagacggaca gcgccaggga gcaatggcag cgcgccgacc 3061 gcgatgggct gtggccaata gcggctgctc agcagggcgc gccgagagca gcggccggga 3121 aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg 3181 gtgttccgca ttctgcaagc ctccggagcg cacgtcggca gtcggctccc tcgttgaccg 3241 aatcaccgac ctctctcccc agcaattcac catgaccgag tacaagccca cggtgcgcct 3301 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta 3361 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca 3421 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg 3481 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga 3541 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga 3601 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt 3661 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc 3721 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt 3781 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac 3841 ctggtgcatg acccgcaagc ccggtgccga aggtagaggt tctctcctca cttgtggtga 3901 tgttgaagaa aaccctggtc caatgtctag actggacaag agcaaagtca taaacggagc 3961 tctggaatta ctcaatggtg tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa 4021 gctgggagtt gagcagccta ccctgtactg gcacgtgaag aacaagcggg ccctgctcga 4081 tgccctgcca atcgagatgc tggacaggca tcatacccac ttctgccccc tggaaggcga 4141 gtcatggcaa gactttctgc ggaacaacgc caagtcatac cgctgtgctc tcctctcaca 4201 tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca gagaaacagt acgaaaccct 4261 ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg cactgtacgc 4321 tctgtccgcc gtgggccact ttacactggg ctgcgtattg gaggaacagg agcatcaagt 4381 agcaaaagag gaaagagaga cacctaccac cgattctatg cccccacttc tgagacaagc 4441 aattgagctg ttcgaccggc agggagccga acctgccttc cttttcggcc tggaactaat 4501 catatgtggc ctggagaaac agctaaagtg cgaaagcggc gggccgaccg acgcccttga 4561 cgattttgac ttagacatgc tcccagccga tgcccttgac gactttgacc ttgatatgct 4621 gcctgctgac gctcttgacg attttgacct tgacatgctc cccgggtaac taagtaagga 4681 tcgatccaag atatcgtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg 4741 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt 4801 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg 4861 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc 4921 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg 4981 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt 5041 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc 5101 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg 5161 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga 5221 tctccctttg ggccgcctcc ccgcctgttt cgcctcggcg tccggactag aggtaccttt 5281 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg 5341 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc 5401 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 5461 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 5521 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag 5581 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag 5641 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 5701 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 5761 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta 5821 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 5881 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 5941 ggcctttcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 6001 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 6061 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 6121 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 6181 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 6241 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 6301 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 6361 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 6421 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 6481 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 6541 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 6601 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 6661 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 6721 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 6781 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 6841 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 6901 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 6961 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 7021 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 7081 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 7141 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 7201 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 7261 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 7321 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 7381 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 7441 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 7501 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 7561 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 7621 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 7681 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 7741 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg 7801 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt 7861 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca 7921 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct 7981 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat 8041 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct 8101 taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac 8161 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg 8221 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat 8281 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct 8341 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc 8401 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg 8461 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc 8521 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct 8581 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 8641 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 8701 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 8761 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 8821 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 8881 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 8941 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 9001 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg 9061 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 9121 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 9181 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc 9241 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 9301 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 9361 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 9421 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 9481 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca 9541 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg 9601 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa 9661 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa 9721 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt 9781 ttcagaccca cctcccaacc ccgaggggac //