LOCUS       Exported                3781 bp ds-DNA     circular SYN 26-JUN-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pYPQ121 (attL1-attR5)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Invitrogen
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 26, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
COMMENT     ORIGDB|Private
            SSBMSPEC|10836|NONE|Private|0
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1080..3773
                     /note="dnas_title=pYPQ121 (attL1-attR5)
                     invitrogen=814
                     vntifkey=98"
     mat_peptide     27..50
                     /label=FLAG tag
                     /note="FLAG"
     sig_peptide     54..74
                     /label=NLS-1
                     /note="NLS-1"
     intron          97..181
                     /note="Intron1"
     sig_peptide     182..202
                     /label=NLS-2
                     /note="NLS-2"
     exon            203..334
                     /label=VP64
                     /note="VP64"
     misc_feature    335..388
                     /label=T2A
     mat_peptide     395..421
                     /label=HA tag
                     /note="HA"
     sig_peptide     422..442
                     /label=NLS-1
                     /note="NLS-3"
     sig_peptide     483..503
                     /label=NLS-2
                     /note="NLS-2"
     exon            504..635
                     /label=VP64
                     /note="VP64"
     misc_feature    648..926
                     /label=NOS term
                     /note="dnas_title=NOS term
                     vntifkey=21"
     protein_bind    933..937
                     /label=TOPO Cloning site(1)
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_label=TOPO Cloning site
                     ApEinfo_revcolor=pink
                     dnas_title=TOPO Cloning site(1)
                     invitrogen=2460000
                     vntifkey=31"
     misc_feature    933
                     /label=overhang
                     /label=3'-T overhang
                     /label=overhang
                     /note="ApEinfo_fwdcolor=pink
                     ApEinfo_revcolor=pink
                     dnas_title=3'-T overhang
                     vntifkey=274"
     misc_recomb     946..1069
                     /label=attR5
                     /note="dnas_title=attR5
                     invitrogen=2703000
                     vntifkey=86"
     promoter        1198..1337
                     /label=Spn promoter
                     /note="dnas_title=Spn promoter
                     vntifkey=30"
     CDS             1338..2348
                     /codon_start=1
                     /gene="Streptomycin/Spectinomycin adenyltransferase"
                     /label=SpnR
                     /note="dnas_title=SpnR
                     invitrogen=2750000
                     vntifkey=4"
                     /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQAR
                     YAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLLAV
                     HLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTI
                     VVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPA
                     AEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVA
                     ADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2415..3088
                     /direction=RIGHT
                     /gene="ori"
                     /label=pUC origin
                     /note="dnas_title=pUC origin
                     invitrogen=2530000
                     vntifkey=33"
     terminator      3359..3386
                     /label=rrnB T2 transcription terminator
                     /note="dnas_title=rrnB T2 transcription terminator
                     invitrogen=2590000
                     vntifkey=43"
     terminator      3518..3561
                     /label=rrnB T1 terminatior
                     /note="dnas_title=rrnB T1 terminatior
                     vntifkey=43"
     misc_recomb     3660..3759
                     /label=attL1
                     /note="dnas_title=attL1
                     invitrogen=2670000
                     vntifkey=86"
ORIGIN
        1 gcgaagcttg gcttggtacc gatatggact acaaagacga tgacgataaa gctcctaaga
       61 aaaagcgcaa agtcccctct agataacgca ggatccgtaa gtttatcagt taaatataat
      121 aaataaagaa gaaaaccaaa aaaatggcta actaaaacga tggtcttatg attttatgca
      181 gccaaaaaag aaacgtaagg ttgatgctct tgacgatttt gacctcgata tgctcgacgc
      241 tcttgatgat tttgatctcg acatgctcga tgcacttgat gactttgacc ttgacatgct
      301 cgacgcactc gatgacttcg acctcgacat gcttgagggc agaggaagtc ttctaacatg
      361 cggtgacgtg gaggagaatc ccggccctat cgattaccct tatgatgtgc cagactacgc
      421 tcctaaaaag aagcgcaaag ttggcattca cggggtgccg gctagctaat aggcttagat
      481 ctccaaaaaa gaaacgtaag gttgatgctc ttgacgattt tgacctcgat atgctcgacg
      541 ctcttgatga ttttgatctc gacatgctcg atgcacttga tgactttgac cttgacatgc
      601 tcgacgcact cgatgacttc gacctcgaca tgctttaata ggacgtccga tcgttcaaac
      661 atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata
      721 taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt
      781 atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac
      841 aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat
      901 cgggaattga tcccccctcg acagcttccg gaaagggcga attcgcaact ttgtatacaa
      961 aagttgaacg agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata
     1021 aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgc catccagctg
     1081 atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg
     1141 tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca tgcctcctct
     1201 ggaccagcca ggacagaaat gcctcgactt cgctgctacc caaggttgcc gggtgacgca
     1261 caccgtggaa acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc
     1321 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
     1381 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt
     1441 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
     1501 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatga
     1561 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
     1621 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
     1681 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
     1741 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
     1801 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
     1861 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
     1921 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
     1981 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
     2041 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
     2101 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
     2161 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
     2221 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
     2281 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg
     2341 gcaaataacc ctcgagccac ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc
     2401 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     2461 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     2521 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     2581 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2641 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2701 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     2761 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     2821 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2881 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2941 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3001 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3061 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     3121 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     3181 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     3241 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     3301 gtgagcgcaa cgcaattaat acgcgtaccg cgagccagga agagtttgta gaaacgcaaa
     3361 aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg
     3421 tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc ccggcggatt
     3481 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttc
     3541 cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg ttaacgcttg
     3601 catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag ctcgggcccc
     3661 aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat
     3721 gcttttttat aatgccaact ttgtacaaaa aagcaggctc cgaattcgcc cttcaccatg
     3781 g
//
