LOCUS       pDB070.iodo5_act        4682 bp    DNA     circular UNA 05-JUL-2017
DEFINITION  .
ACCESSION   urn.local...1j-7rxwani
VERSION     urn.local...1j-7rxwani
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     rep_origin      1..913
                     /created_by="User"
                     /label="p15A_origin"
     CDS             complement(974..1597)
                     /note="TetR"
                     /label="TetR CDS"
     promoter        complement(1611..1653)
                     /note="PN25 promoter"
                     /label="PN25 promoter"
     protein_bind    complement(1705..1723)
                     /created_by="User"
                     /label="TetR binding site-O2"
     promoter        1724..1759
                     /created_by="User"
                     /label="Tet-regulated promoter"
     protein_bind    1729..1747
                     /created_by="User"
                     /label="TetR binding site-O1"
     protein_bind    complement(1759..1777)
                     /created_by="User"
                     /label="TetR binding site-O2"
     CDS             1785..2705
                     /created_by="User"
                     /label="pNiF aaRS"
     terminator      2786..2943
                     /created_by="User"
                     /label="rrnB_terminator"
     promoter        3263..3413
                     /created_by="User"
                     /label="proK"
     tRNA            3442..3518
                     /created_by="User"
                     /label="optimized tRNA"
     CDS             complement(3675..4334)
                     /created_by="User"
                     /label="CAT/CamR"
ORIGIN      
        1 ccgccggaca tcagcgctag cggagtgtat actggcttac tatgttggca ctgatgaggg
       61 tgtcagtgaa gtgcttcatg tggcaggaga aaaaaggctg caccggtgcg tcagcagaat
      121 atgtgataca ggatatattc cgcttcctcg ctcactgact cgctacgctc ggtcgttcga
      181 ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt tcctggaaga tgccaggaag
      241 atacttaaca gggaagtgag agggccgcgg caaagccgtt tttccatagg ctccgccccc
      301 ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg acaggactat
      361 aaagatacca ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt cctgcctttc
      421 ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc tcattccacg cctgacactc
      481 agttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc cgttcagtcc
      541 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggaaag acatgcaaaa
      601 gcaccactgg cagcagccac tggtaattga tttagaggag ttagtcttga agtcatgcgc
      661 cggttaaggc taaactgaaa ggacaagttt tggtgactgc gctcctccaa gccagttacc
      721 tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa ccgccctgca aggcggtttt
      781 ttcgttttca gagcaagaga ttacgcgcag accaaaacga tctcaagaag atcatcttat
      841 taatcagata aaatatttct agatttcagt gcaatttatc tcttcaaatg tagcacctga
      901 agtcagcccc atacgatata agttgtaatt ctcatgtttg acagcttatc atcgataaag
      961 ctatctagaa agcttaagac ccactttcac atttaagttg tttttctaat ccgcatatga
     1021 tcaattcaag gccgaataag aaggctggct ctgcaccttg gtgatcaaat aattcgatag
     1081 cttgtcgtaa taatggcggc atactatcag tagtaggtgt ttccctttct tctttagcga
     1141 cttgatgctc ttgatcttcc aatacgcaac ctaaagtaaa atgccccact gcgctgagtg
     1201 catataatgc attctctagt gaaaaacctt gttggcataa aaaggctaat tgattttcga
     1261 gagtttcata ctgtttttct gtaggccgtg tacctaaatg tacttttgct ccatcgcgat
     1321 gacttagtaa agcacatcta aaacttttag cgttattacg taaaaaatct tgccagcttt
     1381 ccccttctaa agggcaaaag tgagtatggt gcctatctaa catctcaatg gctaaggcgt
     1441 cgagcaaagc ccgcttattt tttacatgcc aatacaatgt aggctgctct acacctagct
     1501 tctgggcgag tttacgggtt gttaaacctt cgattccgac ctcattaagc agctctaatg
     1561 cgctgttaat cactttactt ttatctaatc tagacatatg aattcggggc tttatgaatc
     1621 tattatacag aaaaattttc ctgaaagcaa ataattaatt gcgttgcgca ttctagagca
     1681 cagctccatc gaatggccag atgatcccta tcagtgatag agattgacac tctatcattg
     1741 atagagttat tttaccactc cctatcagtg atagagaaag acctatggac gaatttgaaa
     1801 tgataaagag aaacacatct gaaattatca gcgaggaaga gttaagagag gttttaaaaa
     1861 aagatgaaaa atctgctctg ataggttttg aaccaagtgg taaaatacat ttagggcatt
     1921 atctccaaat aaaaaagatg attgatttac aaaatgctgg atttgatata attatattgt
     1981 tggctgattt ccacgcctat ttaaaccaga aaggagagtt ggatgagatt agaaaaatag
     2041 gagattataa caaaaaagtt tttgaagcaa tggggttaaa ggcaaaatat gtttatggaa
     2101 gttcgttcca gcttgataag gattatacac tgaatgtcta tagattggct ttaaaaacta
     2161 ccttaaaaag agcaagaagg agtatggaac ttatagcaag agaggatgaa aatccaaagg
     2221 ttgctgaagt tatctatcca ataatgcagg ttaatcctct taattatgag ggcgttgatg
     2281 ttgcagttgg agggatggag cagagaaaaa tacacatgtt agcaagggag cttttaccaa
     2341 aaaaggttgt ttgtattcac aaccctgtct taacgggttt ggatggagaa ggaaagatga
     2401 gttcttcaaa agggaatttt atagctgttg atgactctcc agaagagatt agggctaaga
     2461 taaagaaagc atactgccca gctggaattg ttgaaggaaa tccaataatg gagatagcta
     2521 aatacttcct tgaatatcct ttaaccataa aaaggccaga aaaatttggt ggagatttga
     2581 cagttaatag ctatgaggag ttagagagtt tatttaaaaa taaggaattg catccaatgc
     2641 gcttaaaaaa tgctgtagct gaagaactta taaagatttt agagccaatt agaaagagat
     2701 tataagtctc cagcttggct gttttggcgg atgagagaag attttcagcc tgatacagat
     2761 taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca gtagcgcggt
     2821 ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt
     2881 ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt
     2941 cgaaagactg ggcctttcgt tttatctgtt gtttgtgagc tcccggtcat caatcatccc
     3001 cataatcctt gttagattat caaagtttca aacgctaaat tgcctgatgc gctacgctta
     3061 tcaggcctac atgatctctg caatatattg agtttgcgtg cttttgtagg ccggataagg
     3121 cgttcacgcc gcatccggca agaaacagca aacaatccaa aacgccgcgt tcagcggcgt
     3181 tttttctgct tttcttcgcg aattaattcc gcttcgcaac atgtgagcac cggtttattg
     3241 actaccggaa gcagtgtgac cgtgtgcttc tcaaatgcct gaggccagtt tgctcaggct
     3301 ctccccgtgg aggtaataat tgacgatatg atcagtgcac ggctaactaa gcggcctgct
     3361 gactttctcg ccgatcaaaa ggcattttgc tattaaggga ttgacgaggg cgtatctgcg
     3421 cagtaagatg cgccccgcat tccggcggta gttcagcagg gcagaacggc ggactctaaa
     3481 tccgcatggc aggggttcaa atcccctccg ccggaccaaa ttcgaaaagc ctgctcaacg
     3541 agcaggcttt tttgcatgct cgagcagctc agggtcgaat ttgctttcga atttctgcca
     3601 ttcatccgct tattatcact tattcaggcg tagcaaccag gcgtttaagg gcaccaataa
     3661 ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta
     3721 agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc
     3781 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
     3841 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag
     3901 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
     3961 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
     4021 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
     4081 catatcacca gctcaccgtc tttcattgcc atacggaatt ccggatgagc attcatcagg
     4141 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
     4201 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
     4261 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
     4321 atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg
     4381 cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg ccgatcaacg
     4441 tctcattttc gccaaaagtt ggcccagggc ttcccggtat caacagggac accaggattt
     4501 atttattctg cgaagtgatc ttccgtcaca ggtatttatt cggcgcaaag tgcgtcgggt
     4561 gatgctgcca acttactgat ttagtgtatg atggtgtttt tgaggtgctc cagtggcttc
     4621 tgtttctatc agctgtccct cctgttcagc tactgacggg gtggtgcgta acggcaaaag
     4681 ca
//
