LOCUS       Exported                4552 bp ds-DNA     circular SYN 13-JUL-2017
DEFINITION  -.
ACCESSION   -
VERSION     .
KEYWORDS    -
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 13, 2017 from SnapGene Viewer 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(1..861)
                     /label=bla
     misc_difference 1078..2391
                     /label=Transposon
     CDS             1341..2078
                     /codon_start=1
                     /gene="ErmR"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTT
                     KLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIPYH
                     LSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFH
                     PKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNN
                     LSTVTYEQVLSIFNSYLLFNGRK"
     promoter        2393..2696
                     /label=nrdR promoter
                     /label=insert
                     /note="source=nrdR (VF_0699) promoter"
     CDS             2698..3744
                     /codon_start=1
                     /label=himar1C9
                     /translation="MEKKEFRVLIKYCFLKGKNTVEAKTWLDNEFPDSAPGKSTIIDWY
                     AKFKRGEMSTEDGERSGRPKEVVTDENIKKIHKMILNDRKMKLIEIAEALKISKERVGH
                     IIHQYLDMRKLCAKWVPRELTFDQKQRRVDDSKRCLQLLTRNTPEFFRRYVTMDETWLH
                     HYTPESNRQSAEWTATGEPSPKRGKTQKSAGKVMASVFWDAHGIIFIDYLEKGKTINSD
                     YYMALLERLKVEIAAKRPHMKKKKVLFHQDNAPCHKSLRTMAKIHELGFELLPHPPYSP
                     DLAPSDFFLFSDLKRMLAGKKFGCNEEVIAETEAYFEAKPKEYYQNGIKKLEGRYNRCI
                     ALEGNYVE"
     misc_feature    3773..4546
                     /label=RP4/oriT/oriR6K
ORIGIN
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      121 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      961 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     1021 attaacctat aaaaataggc gtatcacgag gccctttcgt cacgcgtcct cggtacctaa
     1081 caggttggat gataagtccc cggtcttcgt atgccgtctt ctgcttggcg cgccctcgag
     1141 ggttcgtgtt cgtgctgact tgcaccatat cataaaaatc gaaacagcaa agaatggcgg
     1201 aaacgtaaaa gaagttatgg aaataagact tagaagcaaa cttaagagtg tgttgatagt
     1261 gcagtatctt aaaattttgt ataataggaa ttgaagttaa attagatgct aaaaatttgt
     1321 aattaagaag gagtgattac atgaacaaaa atataaaata ttctcaaaac tttttaacga
     1381 gtgaaaaagt actcaaccaa ataataaaac aattgaattt aaaagaaacc gataccgttt
     1441 acgaaattgg aacaggtaaa gggcatttaa cgacgaaact ggctaaaata agtaaacagg
     1501 taacgtctat tgaattagac agtcatctat tcaacttatc gtcagaaaaa ttaaaactga
     1561 atactcgtgt cactttaatt caccaagata ttctacagtt tcaattccct aacaaacaga
     1621 ggtataaaat tgttgggagt attccttacc atttaagcac acaaattatt aaaaaagtgg
     1681 tttttgaaag ccatgcgtct gacatctatc tgattgttga agaaggattc tacaagcgta
     1741 ccttggatat tcaccgaaca ctagggttgc tcttgcacac tcaagtctcg attcagcaat
     1801 tgcttaagct gccagcggaa tgctttcatc ctaaaccaaa agtaaacagt gtcttaataa
     1861 aacttacccg ccataccaca gatgttccag ataaatattg gaagctatat acgtactttg
     1921 tttcaaaatg ggtcaatcga gaatatcgtc aactgtttac taaaaatcag tttcatcaag
     1981 caatgaaaca cgccaaagta aacaatttaa gtaccgttac ttatgagcaa gtattgtcta
     2041 tttttaatag ttatctatta tttaacggga ggaaataatc tagaatgcga gagtagggaa
     2101 ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     2161 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg
     2221 ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact gccaggcatc
     2281 aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacct gcagggcgcg
     2341 ccaagcagaa gacggcatac gaagaccggg gacttatcat ccaacctgtt aggatccgta
     2401 attggcatca taaacatttt cattttcttg taataacagt tgaaataaga atgttgagat
     2461 aatcaatgtc ggtagaagtg atatgattaa taatcgatgt tttatacttt taaatacaga
     2521 caaaatcata gttaaccttt taattaatgt gggtatatac atttatcggt tgcattaatt
     2581 gatactttaa taattaaaac tgggtttttc ttttggttta aacaggaact taatgaaggg
     2641 acataggcag gcatctgctt ttcttgtaca ctaattgaaa aaactaggag gcaacatatg
     2701 gaaaaaaagg aatttcgtgt tttgataaaa tactgttttc tgaagggaaa aaatacagtg
     2761 gaagcaaaaa cttggcttga taatgagttt ccggactctg ccccagggaa atcaacaata
     2821 attgattggt atgcaaaatt caagcgtggt gaaatgagca cggaggacgg tgaacgcagt
     2881 ggacgcccga aagaggtggt taccgacgaa aacatcaaaa aaatccacaa aatgattttg
     2941 aatgaccgta aaatgaagtt gatcgagata gcagaggcct taaagatatc aaaggaacgt
     3001 gttggtcata tcattcatca atatttggat atgcggaagc tctgtgcgaa atgggtgccg
     3061 cgcgagctca catttgacca aaaacaacga cgtgttgatg attctaagcg gtgtttgcag
     3121 ctgttaactc gtaatacacc cgagtttttc cgtcgatatg tgacaatgga tgaaacatgg
     3181 ctccatcact acactcctga gtccaatcga cagtcggctg agtggacagc gaccggtgaa
     3241 ccgtctccga agcgtggaaa gactcaaaag tccgctggca aagtaatggc ctctgttttt
     3301 tgggatgcgc atggaataat ttttatcgat tatcttgaga agggaaaaac catcaacagt
     3361 gactattata tggcgttatt ggagcgtttg aaggtcgaaa tcgcggcaaa acggccccac
     3421 atgaagaaga aaaaagtgtt gttccaccaa gacaacgcac cgtgccacaa gtcattgaga
     3481 acgatggcaa aaattcatga attgggcttc gaattgcttc cccacccgcc gtattctcca
     3541 gatctggccc ccagcgactt tttcttgttc tcagacctca aaaggatgct cgcagggaaa
     3601 aaatttggct gcaatgaaga ggtgatcgcc gaaactgagg cctattttga ggcaaaaccg
     3661 aaggagtact accaaaatgg tatcaaaaaa ttggaaggtc gttataatcg ttgtatcgct
     3721 cttgaaggga actatgttga ataagcggcc gccaccgcgg tggaggggaa ttcccatgtc
     3781 agccgttaag tgttcctgtg tcactcaaaa ttgctttgag aggctctaag ggcttctcag
     3841 tgcgttacat ccctggcttg ttgtccacaa ccgttaaacc ttaaaagctt taaaagcctt
     3901 atatattctt ttttttctta taaaacttaa aaccttagag gctatttaag ttgctgattt
     3961 atattaattt tattgttcaa acatgagagc ttagtacgtg aaacatgaga gcttagtacg
     4021 ttagccatga gagcttagta cgttagccat gagggtttag ttcgttaaac atgagagctt
     4081 agtacgttaa acatgagagc ttagtacgtg aaacatgaga gcttagtacg tactatcaac
     4141 aggttgaact gctgatcttc agatcctcta cgccggacgc atcgtggccg gatcaattcc
     4201 gttttccgct gcataaccct gcttcggggt cattatagcg attttttcgg tatatccatc
     4261 ctttttcgca cgatatacag gattttgcca aagggttcgt gtagactttc cttggtgtat
     4321 ccaacggcgt cagccgggca ggataggtga agtaggccca cccgcgagcg ggtgttcctt
     4381 cttcactgtc ccttattcgc acctggcggt gctcaacggg aatcctgctc tgcgaggctg
     4441 gccggctacc gccggcgtaa cagatgaggg caagcggatg gctgatgaaa ccaagccaac
     4501 caggaagggc agcccaccta tcacggaatt gatccccctc gaattgacgc gt
//
