LOCUS       pMK17-parBp_(pMK17-01)      9538 bp    DNA     circular UNA 
DEFINITION  Ligation of pMK14-parBp* into pMK17*
FEATURES             Location/Qualifiers
     CDS             334..790
                     /label='czcD'
     CDS             790
                     /label=ORF-5''
     promoter        822..827
                     /label=RBS
     promoter        834..836
                     /label=start
     CDS             834..2318
                     /label=parBp-sfmGFP
     CDS             834..1559
                     /label=parB
     CDS             1560
                     /label=linker'
     CDS             1609..2319
                     /label='gfp_sfDSM''
     promoter        2348..2436
                     /label=TER_rrnB
     promoter        2441..2492
                     /label=TER_rpsI
     promoter        2497..2524
                     /label=TER_tufA
     CDS             2545..2589
                     /note="promoterless and AUG-less 'lacZ"
                     /label='lacZ
     CDS             2654..3466
                     /note="end of pneumo ?-Gal"
                     /label='bgaA
     CDS             complement(4695..5556)
                     /note=?-lactamase
                     /label=bla
     CDS             5765..6700
                     /note="EII galactitol"
                     /label='gatC
     CDS             complement(7128..9062)
                     /note="tetracycline resistance"
                     /label=tetM
ORIGIN
        1 CTAGCGCTAT ATGCGTTGAT GCAATTTCTA TGCGCACCCG TTCTCGGAGC ACTGTCCGAC
       61 CGCTTTGGCC GCCGCCCAGT CCTGCTCGCT TCGCTACTTG GAGCCACTAT CGACTACGCG
      121 ATCATGGCGA CCACACCCGT CCTGTGGATC TATCGATGCA TGCAGCCGAC GGTACCGCAT
      181 GCTGCTGATA CGGCGGTCAA TGTTAGTCAT ATGGACACTT AAGGCAAATT GTTCAGAACT
      241 GAATAAAGCT GACGTTTTGC TTCTATCCTT TCTTTGAGTT TTAGTGGATA ATGATAATGA
      301 ACAAGGTGTT CATAAATCTA TTATAACAAA GGAATGAGAA ATATGAAGGC AAAATATGCT
      361 GTTTGGGTGG CTTTTTTCTT AAATTTGACT TATGCCATTG TTGAGTTTAT TGCAGGTGGA
      421 GTATTTGGTT CTAGCGCTGT TCTTGCTGAC TCTGTGCATG ACTTGGGAGA TGCGATTGCA
      481 ATTGGAATAT CAGCTTTTGT AGAAACAATC TCCAATCGTG AAGAAGACAA TCAGTACACC
      541 TTGGGCTATA AGCGGTTTAG CCTGCTAGGA GCCTTAGTAA CAGCTGTGAT TCTCGTAACG
      601 GGCTCTGTTC TAGTCATTTT GGAAAATGTC ACGAAGATTT TGCATCCGCA ACCAGTCAAT
      661 GATGAGGGGA TTCTCTGGTT AGGAATTATT GCGATTACTA TCAATCTGTT AACGAGTCTG
      721 GTGGTTGGTA AGGGAAAGAC AAAGAATGAG TCTATTCTGA GTCTGCGGAT CTTAAGGAGG
      781 CAAATATGAG AATTCGGATC TCGAGTACTG ATTAACTAAT AAGGAGGACA AACATGAGTA
      841 ATAGTTTCGG ATTTACAGAC CTTATGAACA AGGACGAACA CAAGCGAAAA AAAACAAATA
      901 CAAAAAATAT TCCTATTGAG GAGATAAAGG AAAATGAGAA TAACAACTAT GATTTAGTTG
      961 ACATTGATAA GTTAGCTGAT AGCATAGACG AGTTAGGCTT ATTACAACCC GTTCTTGTTA
     1021 AACAACGTGA TAAATATAGC TATGAATTGA TTGCAGGGCA TAGACGATTT AATGCTATTA
     1081 AAAAACTAAT TAGTGAAAAT AGACTCCCTG AAGATTACGA AGTTCTCGCT AAGAAAGTTG
     1141 ATGAAGACGA GGACGAGCTT GTAACAAGAC TAAAGTTGCA TGAAACTAAC CTACAAACTC
     1201 GCTCGCTCTT AAAAATGCCA GAAGAAGAAA AAATAGCCAT CATTGATGAC TATATGGATA
     1261 TTTTAGATAA AGCCAAAAAA CAAGGGGTAC AAATCAACGG AAAACCTGTT AAAGGAAAGA
     1321 CTAGGGACTT AATTGCCGAA AGATTTGGTA TCAGTCATTA TACCGCTCAA AAACTGATTA
     1381 GAAAAGCTAA AGAACAAGGT GGTGAAGAAG AGGGGGCTAA ATTTAGCCCC CAAAAGAAAA
     1441 CCGCTAAAAA GCCAATCACG CAGTTAAAGA AAATTGAAAC TCAATTAGAA AAATTAGAGT
     1501 TTGAGGGAAC GGAAGAAGAA CAAGAAATCA AGAAAAAATT GATTGAGTTG TTGATGAAAT
     1561 CTAGTTCTAG AGGATCTGGT GGAGAAGCTG CAGCTAAAGC TGGATCAAAA GGAGAAGAGC
     1621 TGTTCACAGG TGTTGTGCCG ATTCTCGTTG AGCTTGACGG AGATGTAAAC GGACACAAAT
     1681 TCTCTGTTCG CGGTGAAGGT GAAGGAGATG CAACAAACGG CAAGCTGACA TTGAAGTTTA
     1741 TTTGCACAAC TGGAAAGCTG CCGGTTCCTT GGCCGACACT TGTAACGACG CTGACTTACG
     1801 GCGTTCAATG CTTCTCTCGT TATCCAGACC ACATGAAACG CCATGATTTC TTCAAATCTG
     1861 CAATGCCTGA AGGCTACGTT CAAGAGCGTA CGATCAGCTT CAAAGATGAC GGAACGTACA
     1921 AAACAAGAGC AGAAGTGAAG TTTGAAGGTG ACACACTTGT GAACCGCATT GAATTGAAAG
     1981 GCATTGATTT CAAAGAAGAT GGAAACATCC TTGGACACAA ACTTGAATAC AACTTCAACA
     2041 GCCACAACGT ATACATCACT GCTGACAAAC AAAAAAACGG CATCAAAGCA AACTTCAAAA
     2101 TCCGTCATAA CGTAGAGGAC GGTTCTGTTC AGCTTGCTGA TCATTATCAG CAAAATACAC
     2161 CGATCGGTGA CGGCCCGGTT CTTCTTCCTG ATAACCATTA TTTATCAACT CAAAGCAAAT
     2221 TATCAAAAGA CCCAAATGAA AAGCGTGACC ACATGGTGCT GCTTGAATTT GTGACAGCTG
     2281 CTGGTATCAC TCACGGCATG GATGAGCTTT ATAAGTAAGC TAGTCAAGGT CGGCAATTCT
     2341 GCAGTACTAG GACGCCGCCA AGCCAGCTTA AACCCAGCTC AATGAGCTGG GTTTTTTGTT
     2401 TGTTAAAAAT GAAGAAGAAA CTGTGAAGCG TATTTATAGC AAAGCACTCA AAAGTTTACC
     2461 TTATGGGTGC TTTTTTCGTG CTTTTTTGAA AAGACAAAAA AAAGAACCTT GCCAAGCAAG
     2521 ATTCTTTTGA TAGCGCTATC GCTGAGCGCC GGTCGCTACC ATTACCAGTT GGTCTGGTGT
     2581 CAAAAATAAT AATAACCGGG CAGGCCATGT CTGCCCGTAT TTCGCGTAAG GAAATCCATT
     2641 ATGTACTATT TCTGGTGATG AAATCAACGT AACATTTAAA GCTGTCAAAG CCAAAGTCAT
     2701 GAGATGGCGT ATGGAGCGTA AAGCTGACAA GAGCGGTGTT GCGATGATTG AGATGACCTT
     2761 CCTTGCACCA AGTGAATTGC CTCAAGAAAG CACTCAATCA AAGATTCTTG TAGATGGAAA
     2821 AGAACTTGCT GATTTCGCTG AAAATCGTCA AGACTATCAA ATTACCTATA AAGGTCAACG
     2881 GCCAAAAGTC TCAGTTGAAG AAAACAATCA AGTAGCTTCA ACTGTGGTAG ATAGTGGAGA
     2941 AGATAGCCTT CCAGTACTTG TTCGCCTCGT TTCAGAAAGT GGAAAACAAG TCAAGGAATA
     3001 CCGTATCCAG TTGACTAAGG AAAAACCAGT TTCTGCTGTA CAAGAAGATC TTCCAAAACT
     3061 CGAATTTGTT GAAAAAGATT TGGCCTACAA GACAGTTGAG AAAAAAGATT CAACACTGTA
     3121 TCTAGGTGAA ACTCGTGTAG AACAAGAAGG AAAAGTTGGA AAAGAACGTA TCTTTACAGT
     3181 GATTAATCCT GATGGAAGTA AGGAAGAAAA ACTCCGTGAA GTGGTAGAAG TTCCGACAGA
     3241 CCGCATCGTC TTGGTTGGAA CCAAACCAGT AGCTCAAGAA GCTAAAAAAC CACAAGTGTC
     3301 AGAAAAAGCA GATACAAAAC CAATTGATTC AAGTGAAGCT GATCAAACTA ATAAAGCCCA
     3361 GTTACCAAAT ACAGGTAGTG CGGCAAGCCA AGCAGCAGTA GCAGCAGGTT TAGCTCTTCT
     3421 AGGTTTGAGT GCAGGATTAG TAGTTACTAA AGGTAAAAAA GAAGACTAGC TGCCTCGCGC
     3481 GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT
     3541 GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG
     3601 GGTGTCGGGG CGCAGCCATG ACCCAGTCAC GTAGCGATAG CGGAGTGTAT ACTGGCTTAA
     3661 CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGCGGTGTG AAATACCGCA
     3721 CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGCTCTTCC GCTTCCTCGC TCACTGACTC
     3781 GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG
     3841 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA
     3901 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA
     3961 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG
     4021 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT
     4081 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG
     4141 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC
     4201 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT
     4261 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA
     4321 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC
     4381 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC
     4441 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT
     4501 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC
     4561 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT
     4621 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA
     4681 AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT
     4741 ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG
     4801 CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA
     4861 TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT
     4921 ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT
     4981 TAATAGTTTG CGCAACGTTG TTGCCATTGC TGCAGGCATC GTGGTGTCAC GCTCGTCGTT
     5041 TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT
     5101 GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC
     5161 CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC
     5221 CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT
     5281 GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AACACGGGAT AATACCGCGC CACATAGCAG
     5341 AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT
     5401 ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC
     5461 TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA
     5521 GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG
     5581 AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA
     5641 TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TCTAAGAAAC
     5701 CATTATTATC ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTTCA
     5761 AGAATTAATT CCTTCTTAAC GCCCCAAGTT CATCACCAAT GACATCAACT CACATGAACT
     5821 ACATGATGAA CCCAGTTATC ATGGTTTTGG ATAAGATTTT TGAAAAATTC TTCCCAGGCC
     5881 TTGATAAATA TGACTTTGAT GCTGCTAAAT TGAACAAGAA AATCGGTTTC TGGGGATCTA
     5941 AATTCTTCAT CGGTTTCATC CTTGGTATCG TTATCGGTAT TATGGGAACT CCACATCCAA
     6001 TTGCAGGTGT TGCAGATGCA GATAAATGGC GTCTTGTTAT CAAAGGATGG TTGTCTCTTG
     6061 GTTTGACTGC CGGTGTATCT TTGGAACTCT TCTCACTTAT CGGTTCATGG TTCATCGCAG
     6121 CCGTAGAACC ACTATCACAA GGTATTACAA ACGTTGCTAC TAAACGTCTT CAAGGACGTA
     6181 AATTCAATAT CGGTCTTGAC TGGCCATTCA TCGCTGGTCG TGCTGAAATC TGGGCTTGTG
     6241 CCAACGTACT TGCACCAATC ATGTTGATTG AAGCAGTGCT TCTTTCAAAA GTTGGAAATG
     6301 GTATCTTGCC ACTTGCAGGT ATCATCGCTA TGGGTGTTAC TCCAGCTCTC TTGGTTGTAA
     6361 CTCGTGGTAA ATTGCTCCGT ATGATTATCT TCGGAACACT CTTGTTGCCA CTCTTCCTTC
     6421 TTTCAGGTAC ACTTATTGCA CCATTTGCAA CAGAACTTGC TAAAGGTGTA GGTGCCTTCC
     6481 CAGAAGGTGT GAGCCAAACT CAATTGATTA CTCACTCTAC TCTTGAAGGA CCAATCGAAA
     6541 AACTTCTTGG TTGGACAATT GGTAACACTA CAACTGGTGA TATCAAAGCA ATCCTTGGTG
     6601 CAGTAGCCTT CCTTGTATTC TATATCGGTA TCTTTGCTTG GTACAGAAAA CAAATGATCA
     6661 AACGTAACGA AGAGTACGCA GCAAAAGCAA AATAATGCGC TCCGCTAGCT TTACAGACAA
     6721 AGAACTATCC TTAATGGAAG CACTAGCAAA AGGTATCAAT GAAGCAAGAA ATATTGAAGG
     6781 CTAGTCAGTA AAATTCAGAT AAACAAAAAG AGCCGATAAG ATGAGAGTTT CATTTCTCAA
     6841 ATTATCGGCT CTGCGTCTTG GCGTCTGGCT CTTTGATTGT TATTATATTC ATTTTTTGAA
     6901 CATTAATTAA AGTTTCGTTC TTACATTTGG GGCAGTATAA AGGGAAATTT TTAAGTATAG
     6961 TATCCACTCG TATTCGTAGT CTTGTTTTAT TTCCACAAAT AGGACACAAT ATCCACTTGT
     7021 AGTTTATAAT AACAATCTCC TCCTTTCCAC TTTAATTCAA ATCTATATTA AAGAATATTT
     7081 CATCTTATTT AATAAGAAAC CATATTTATA TAACAACATA AAACGCACTA AGTTATTTTA
     7141 TTGAACATAT ATCTTACTTT ATCTATCTGA CTATTTAGAC GACGGGTCTG GCAAACAGGT
     7201 TCGCCAGTGG TAACCTGATA TCCTTTTAGC TCTGCTAAAC AAACACTAAG CCCATTTGTA
     7261 AAAAAAGTTA AATCATTGCG ATAATCTTGA ATACATCGAG CAGGAATTTC TCCAATAATA
     7321 ATGACCTCAT TATTTTTCAG TTGAGTATTT ACGATATTTG CACAATATTT GGGAGCATCG
     7381 TTATATGCCC GTGAAAGATA TTCCTGTGGT GCATAAACTT TAAAACTAAG ATATGGCTCT
     7441 AACAATTCTG TTCCAGCTTT TCTAAAGGCT TGCTCCAATA CAATAGGAGC AAGCATCCGA
     7501 AAATCTGCTG GGGTACTAAC AGGGCTATAG TATAAGCCAT ACTTAAAACA GATTTTACAG
     7561 TCCGTCACAT TCCAACCATA CAATCCTTGT TCACAACCAT AGCGTATCCC TTCCATAACT
     7621 GCATTTTGAA ATGATTGATT TAAGTATCCA AGAGAAACCG AGCTCTCATA CTGCATTCCA
     7681 CTTCCCAACG GAAGCGGTGA TACAGATAAA CCAATGGAAG CCCAGAAAGG ATTTGGCGGC
     7741 ACTTCGATGT GAATGGTATA TTCTGCATTT TTTAACGGTC TCTCCATATA AATGACTGTA
     7801 GGCTCTTTTA GTTCTATCTC CACATGATAC TTTTCTTGCA ACAGTGCACT AATCACTTCC
     7861 ATTTGTACTT TCCCTAAGAA AGAAAGTATA ATTTCATGTG TCGTAGAATC CACGTAATAT
     7921 CGTAGAAGCG GATCACTATC TGAGATTTCC AAAAGGGCAT CAAGCAACAT TTCTCTCTGT
     7981 TCAGGTTTAC TCGGTTCAAC AGTTGTTTGT AGTAGAGGGT GCGGATTTTC AATCTTTTTT
     8041 CTCTGTGGCA ATAGTTTTGT ATCTCCAAGA ACACTATTTA ACTTCAAAAA CTCATTTTGC
     8101 AAAATAACAA TTTCTCCAGA ATAAGCTCTA TCAATCTTAC ATAATTCACC ATTTATTGAA
     8161 GTATACATTT CTGTAACTTT TATTTTTTCT TTTTCTGATA CTCTAACCGA ATCTCGTAAA
     8221 TGTAGTACTC CACTATAAAG GCGTATATAT GCAAGACGTT GTCTTTTTTT TGTATATTCA
     8281 ATTTTGAAAA CATTTCCGCA AAGTTCAGAC GGACCTCGAT GTGTTGATGA ATAAAATTTA
     8341 TTAGTAATAA CTTCTATAAG GTTATCAATC CCTATATTAC TTTTTGCACT TCCATGATAA
     8401 AGAGGGAACA GAGAACAATT CTGAAATCTT ATGCTTTCCT CTTGTTCGAG TTCCAATGCT
     8461 TCTAATGATT TACCGGACAT ATATTTCTCT AAAAGGTCAT CGTTTCCCTC TATTACCGTA
     8521 TCCCATTGTT CAGATTCGGT AAAGTTCGTC ACACACACAT TAGGATACAG TTCTACCTTC
     8581 TGTTTGATTA CAATTTCGGC AGAAAGTTTC TCTTTAATAT CCTGATAAAC CGTTGATAAA
     8641 TCAATTCCAT TTTGGTCAAT CTTATTGATA AAAAAGATTG TGGGAATCCC CATTTTCCTA
     8701 AGTGCATGAA ATAATATACG AGTTTGTGCT TGTACGCCAT CTTTTGCAGA AATCAGTAGA
     8761 ATTGCCCCAT CTAAAACTGA TAATGAACGA TATACTTCTG CTAAGAAATC CATATGTCCT
     8821 GGCGTGTCTA TGATGTTCAC CTTCGTATTT TCCCACTGAA AAGAGGTTAT TCCTGTCTGA
     8881 ATTGTAATTC CTCTCTGACG TTCTAAAAGC GTATTATCCG TCCTCGTTGT ACCTTTGTCC
     8941 ACGCTTCCTA ATTCTGTAAT CGCTCCACTG TTATATAATA AGCTTTCTGT TAAGGTAGTT
     9001 TTTCCTGCAT CAACATGAGC TAAAACTCCA ATATTAATAA TTTTCATGTG ATTTTCCTCC
     9061 ATTCAAAAGC CCAAAAGGGC ATAAAAATCC CAGTGATAAA TACTCTTATC ACTGGGATTT
     9121 TTATGCATAA CCATAGGCAT ACAAAGCATA CAGATATTCT CCGGATACTT TAGAATCACA
     9181 TGATAAAGGT ATTCTTAAAC TGGGTACAAA AAACTAAGCC CTCCTAAAAA AGGACATCCA
     9241 ATTATTTGTT CCCGCTATCA AATTGACAGT TTATTTAAGA ATACCTTGCC GCATATTTAT
     9301 TAACTCCTTT TAAATAGATA CTTAAATTAT AGCACGTAAG AGCATATTTG TAAAGGAATC
     9361 TCCAATTTTT TATCAAAAAG AGTACATGAT TACAAAGTAT CTGTAATCAT GTACCAATAT
     9421 TTGTTATTTT ACAATCTTCC AATTATCTTG TTTTTCTAAT ATCAACTCAA ATTGCGAGAT
     9481 TTGGGTTGCC TTTGTTTCTT GATCTAAATA TTTCACAGCG ACATTCACGA TTACTTGG
//
