LOCUS       pMK17-parBpx-modified1      9279 bp    DNA     circular UNA 
DEFINITION  Ligation of pMK14-parBp-MUT* into pMK17*
FEATURES             Location/Qualifiers
     CDS             334..531
                     /label='czcD'
     CDS             531
                     /label=ORF-5''
     promoter        563..568
                     /label=RBS
     promoter        575..577
                     /label=start
     CDS             575..1300
                     /label=ParB
     CDS             575..2059
                     /label=parBpx-sfmGFP
     promoter        1154..1170
                     /label=parS-MUT
     CDS             1301
                     /label=linker'
     CDS             1350..2060
                     /label='gfp_sfDSM''
     promoter        2089..2177
                     /label=TER_rrnB
     promoter        2182..2233
                     /label=TER_rpsI
     promoter        2238..2265
                     /label=TER_tufA
     CDS             2286..2330
                     /note="promoterless and AUG-less 'lacZ"
                     /label='lacZ
     CDS             2395..3207
                     /note="end of pneumo ??-Gal"
                     /label='bgaA
     CDS             complement(4436..5297)
                     /note=??-lactamase
                     /label=bla
     CDS             5506..6441
                     /note="EII galactitol"
                     /label='gatC
     CDS             complement(6869..8803)
                     /note="tetracycline resistance"
                     /label=tetM
ORIGIN
        1 CTAGCGCTAT ATGCGTTGAT GCAATTTCTA TGCGCACCCG TTCTCGGAGC ACTGTCCGAC
       61 CGCTTTGGCC GCCGCCCAGT CCTGCTCGCT TCGCTACTTG GAGCCACTAT CGACTACGCG
      121 ATCATGGCGA CCACACCCGT CCTGTGGATC TATCGATGCA TGCAGCCGAC GGTACCGCAT
      181 GCTGCTGATA CGGCGGTCAA TGTTAGTCAT ATGGACACTT AAGGCAAATT GTTCAGAACT
      241 GAATAAAGCT GACGTTTTGC TTCTATCCTT TCTTTGAGTT TTAGTGGATA ATGATAATGA
      301 ACAAGGTGTT CATAAATCTA TTATAACAAA GGAATGAGAA ATATGAAGGC AAAATATGCT
      361 GTTTGGGTGG CTTTTTTCTT AAATTTGACT TATGCCATTG TTGAGTTTAT TGCAGGTGGA
      421 GTATTTGGTT CTAGCGCTGT TCTTGCTGAC TCTGTGCATG ACTTGGGAGA TGCGATTGCA
      481 ATTGGAATAT CAGCTTTTCT AGAACTAGTA GATCTCGCGG CCGCGGATCC GAATTCGGAT
      541 CTCGAGTACT GATTAACTAA TAAGGAGGAC AAACATGAGT AATAGTTTCG GATTTACAGA
      601 CCTTATGAAC AAGGACGAAC ACAAGCGAAA AAAAACAAAT ACAAAAAATA TTCCTATTGA
      661 GGAGATAAAG GAAAATGAGA ATAACAACTA TGATTTAGTT GACATTGATA AGTTAGCTGA
      721 TAGCATAGAC GAGTTAGGCT TATTACAACC CGTTCTTGTT AAACAACGTG ATAAATATAG
      781 CTATGAATTG ATTGCAGGGC ATAGACGATT TAATGCTATT AAAAAACTAA TTAGTGAAAA
      841 TAGACTCCCT GAAGATTACG AAGTTCTCGC TAAGAAAGTT GATGAAGACG AGGACGAGCT
      901 TGTAACAAGA CTAAAGTTGC ATGAAACTAA CCTACAAACT CGCTCGCTCT TAAAAATGCC
      961 AGAAGAAGAA AAAATAGCCA TCATTGATGA CTATATGGAT ATTTTAGATA AAGCCAAAAA
     1021 ACAAGGGGTA CAAATCAACG GAAAACCTGT TAAAGGAAAG ACTAGGGACT TAATTGCCGA
     1081 AAGATTTGGT ATCAGTCATT ATACCGCTCA AAAACTGATT AGAAAAGCTA AAGAACAAGG
     1141 TGGTGAAGAA GAGGGAGCAA AGTTCTCTCC ACAAAAGAAA ACCGCTAAAA AGCCAATCAC
     1201 GCAGTTAAAG AAAATTGAAA CTCAATTAGA AAAATTAGAG TTTGAGGGAA CGGAAGAAGA
     1261 ACAAGAAATC AAGAAAAAAT TGATTGAGTT GTTGATGAAA TCTAGTTCTA GAGGATCTGG
     1321 TGGAGAAGCT GCAGCTAAAG CTGGATCAAA AGGAGAAGAG CTGTTCACAG GTGTTGTGCC
     1381 GATTCTCGTT GAGCTTGACG GAGATGTAAA CGGACACAAA TTCTCTGTTC GCGGTGAAGG
     1441 TGAAGGAGAT GCAACAAACG GCAAGCTGAC ATTGAAGTTT ATTTGCACAA CTGGAAAGCT
     1501 GCCGGTTCCT TGGCCGACAC TTGTAACGAC GCTGACTTAC GGCGTTCAAT GCTTCTCTCG
     1561 TTATCCAGAC CACATGAAAC GCCATGATTT CTTCAAATCT GCAATGCCTG AAGGCTACGT
     1621 TCAAGAGCGT ACGATCAGCT TCAAAGATGA CGGAACGTAC AAAACAAGAG CAGAAGTGAA
     1681 GTTTGAAGGT GACACACTTG TGAACCGCAT TGAATTGAAA GGCATTGATT TCAAAGAAGA
     1741 TGGAAACATC CTTGGACACA AACTTGAATA CAACTTCAAC AGCCACAACG TATACATCAC
     1801 TGCTGACAAA CAAAAAAACG GCATCAAAGC AAACTTCAAA ATCCGTCATA ACGTAGAGGA
     1861 CGGTTCTGTT CAGCTTGCTG ATCATTATCA GCAAAATACA CCGATCGGTG ACGGCCCGGT
     1921 TCTTCTTCCT GATAACCATT ATTTATCAAC TCAAAGCAAA TTATCAAAAG ACCCAAATGA
     1981 AAAGCGTGAC CACATGGTGC TGCTTGAATT TGTGACAGCT GCTGGTATCA CTCACGGCAT
     2041 GGATGAGCTT TATAAGTAAG CTAGTCAAGG TCGGCAATTC TGCAGTACTA GGACGCCGCC
     2101 AAGCCAGCTT AAACCCAGCT CAATGAGCTG GGTTTTTTGT TTGTTAAAAA TGAAGAAGAA
     2161 ACTGTGAAGC GTATTTATAG CAAAGCACTC AAAAGTTTAC CTTATGGGTG CTTTTTTCGT
     2221 GCTTTTTTGA AAAGACAAAA AAAAGAACCT TGCCAAGCAA GATTCTTTTG ATAGCGCTAT
     2281 CGCTGAGCGC CGGTCGCTAC CATTACCAGT TGGTCTGGTG TCAAAAATAA TAATAACCGG
     2341 GCAGGCCATG TCTGCCCGTA TTTCGCGTAA GGAAATCCAT TATGTACTAT TTCTGGTGAT
     2401 GAAATCAACG TAACATTTAA AGCTGTCAAA GCCAAAGTCA TGAGATGGCG TATGGAGCGT
     2461 AAAGCTGACA AGAGCGGTGT TGCGATGATT GAGATGACCT TCCTTGCACC AAGTGAATTG
     2521 CCTCAAGAAA GCACTCAATC AAAGATTCTT GTAGATGGAA AAGAACTTGC TGATTTCGCT
     2581 GAAAATCGTC AAGACTATCA AATTACCTAT AAAGGTCAAC GGCCAAAAGT CTCAGTTGAA
     2641 GAAAACAATC AAGTAGCTTC AACTGTGGTA GATAGTGGAG AAGATAGCCT TCCAGTACTT
     2701 GTTCGCCTCG TTTCAGAAAG TGGAAAACAA GTCAAGGAAT ACCGTATCCA GTTGACTAAG
     2761 GAAAAACCAG TTTCTGCTGT ACAAGAAGAT CTTCCAAAAC TCGAATTTGT TGAAAAAGAT
     2821 TTGGCCTACA AGACAGTTGA GAAAAAAGAT TCAACACTGT ATCTAGGTGA AACTCGTGTA
     2881 GAACAAGAAG GAAAAGTTGG AAAAGAACGT ATCTTTACAG TGATTAATCC TGATGGAAGT
     2941 AAGGAAGAAA AACTCCGTGA AGTGGTAGAA GTTCCGACAG ACCGCATCGT CTTGGTTGGA
     3001 ACCAAACCAG TAGCTCAAGA AGCTAAAAAA CCACAAGTGT CAGAAAAAGC AGATACAAAA
     3061 CCAATTGATT CAAGTGAAGC TGATCAAACT AATAAAGCCC AGTTACCAAA TACAGGTAGT
     3121 GCGGCAAGCC AAGCAGCAGT AGCAGCAGGT TTAGCTCTTC TAGGTTTGAG TGCAGGATTA
     3181 GTAGTTACTA AAGGTAAAAA AGAAGACTAG CTGCCTCGCG CGTTTCGGTG ATGACGGTGA
     3241 AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG
     3301 GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCGCAGCCAT
     3361 GACCCAGTCA CGTAGCGATA GCGGAGTGTA TACTGGCTTA ACTATGCGGC ATCAGAGCAG
     3421 ATTGTACTGA GAGTGCACCA TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA
     3481 TACCGCATCA GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG
     3541 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG
     3601 GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG
     3661 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA
     3721 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT
     3781 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC
     3841 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG
     3901 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC
     3961 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA
     4021 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG
     4081 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT
     4141 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC
     4201 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA
     4261 TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA
     4321 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT
     4381 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC
     4441 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT
     4501 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT
     4561 GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG
     4621 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT
     4681 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT
     4741 GTTGCCATTG CTGCAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC
     4801 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT
     4861 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG
     4921 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG
     4981 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT
     5041 TGCCCGGCGT CAACACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC
     5101 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT
     5161 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT
     5221 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG
     5281 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT
     5341 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG
     5401 CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT CATGACATTA
     5461 ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTTC AAGAATTAAT TCCTTCTTAA
     5521 CGCCCCAAGT TCATCACCAA TGACATCAAC TCACATGAAC TACATGATGA ACCCAGTTAT
     5581 CATGGTTTTG GATAAGATTT TTGAAAAATT CTTCCCAGGC CTTGATAAAT ATGACTTTGA
     5641 TGCTGCTAAA TTGAACAAGA AAATCGGTTT CTGGGGATCT AAATTCTTCA TCGGTTTCAT
     5701 CCTTGGTATC GTTATCGGTA TTATGGGAAC TCCACATCCA ATTGCAGGTG TTGCAGATGC
     5761 AGATAAATGG CGTCTTGTTA TCAAAGGATG GTTGTCTCTT GGTTTGACTG CCGGTGTATC
     5821 TTTGGAACTC TTCTCACTTA TCGGTTCATG GTTCATCGCA GCCGTAGAAC CACTATCACA
     5881 AGGTATTACA AACGTTGCTA CTAAACGTCT TCAAGGACGT AAATTCAATA TCGGTCTTGA
     5941 CTGGCCATTC ATCGCTGGTC GTGCTGAAAT CTGGGCTTGT GCCAACGTAC TTGCACCAAT
     6001 CATGTTGATT GAAGCAGTGC TTCTTTCAAA AGTTGGAAAT GGTATCTTGC CACTTGCAGG
     6061 TATCATCGCT ATGGGTGTTA CTCCAGCTCT CTTGGTTGTA ACTCGTGGTA AATTGCTCCG
     6121 TATGATTATC TTCGGAACAC TCTTGTTGCC ACTCTTCCTT CTTTCAGGTA CACTTATTGC
     6181 ACCATTTGCA ACAGAACTTG CTAAAGGTGT AGGTGCCTTC CCAGAAGGTG TGAGCCAAAC
     6241 TCAATTGATT ACTCACTCTA CTCTTGAAGG ACCAATCGAA AAACTTCTTG GTTGGACAAT
     6301 TGGTAACACT ACAACTGGTG ATATCAAAGC AATCCTTGGT GCAGTAGCCT TCCTTGTATT
     6361 CTATATCGGT ATCTTTGCTT GGTACAGAAA ACAAATGATC AAACGTAACG AAGAGTACGC
     6421 AGCAAAAGCA AAATAATGCG CTCCGCTAGC TTTACAGACA AAGAACTATC CTTAATGGAA
     6481 GCACTAGCAA AAGGTATCAA TGAAGCAAGA AATATTGAAG GCTAGTCAGT AAAATTCAGA
     6541 TAAACAAAAA GAGCCGATAA GATGAGAGTT TCATTTCTCA AATTATCGGC TCTGCGTCTT
     6601 GGCGTCTGGC TCTTTGATTG TTATTATATT CATTTTTTGA ACATTAATTA AAGTTTCGTT
     6661 CTTACATTTG GGGCAGTATA AAGGGAAATT TTTAAGTATA GTATCCACTC GTATTCGTAG
     6721 TCTTGTTTTA TTTCCACAAA TAGGACACAA TATCCACTTG TAGTTTATAA TAACAATCTC
     6781 CTCCTTTCCA CTTTAATTCA AATCTATATT AAAGAATATT TCATCTTATT TAATAAGAAA
     6841 CCATATTTAT ATAACAACAT AAAACGCACT AAGTTATTTT ATTGAACATA TATCTTACTT
     6901 TATCTATCTG ACTATTTAGA CGACGGGTCT GGCAAACAGG TTCGCCAGTG GTAACCTGAT
     6961 ATCCTTTTAG CTCTGCTAAA CAAACACTAA GCCCATTTGT AAAAAAAGTT AAATCATTGC
     7021 GATAATCTTG AATACATCGA GCAGGAATTT CTCCAATAAT AATGACCTCA TTATTTTTCA
     7081 GTTGAGTATT TACGATATTT GCACAATATT TGGGAGCATC GTTATATGCC CGTGAAAGAT
     7141 ATTCCTGTGG TGCATAAACT TTAAAACTAA GATATGGCTC TAACAATTCT GTTCCAGCTT
     7201 TTCTAAAGGC TTGCTCCAAT ACAATAGGAG CAAGCATCCG AAAATCTGCT GGGGTACTAA
     7261 CAGGGCTATA GTATAAGCCA TACTTAAAAC AGATTTTACA GTCCGTCACA TTCCAACCAT
     7321 ACAATCCTTG TTCACAACCA TAGCGTATCC CTTCCATAAC TGCATTTTGA AATGATTGAT
     7381 TTAAGTATCC AAGAGAAACC GAGCTCTCAT ACTGCATTCC ACTTCCCAAC GGAAGCGGTG
     7441 ATACAGATAA ACCAATGGAA GCCCAGAAAG GATTTGGCGG CACTTCGATG TGAATGGTAT
     7501 ATTCTGCATT TTTTAACGGT CTCTCCATAT AAATGACTGT AGGCTCTTTT AGTTCTATCT
     7561 CCACATGATA CTTTTCTTGC AACAGTGCAC TAATCACTTC CATTTGTACT TTCCCTAAGA
     7621 AAGAAAGTAT AATTTCATGT GTCGTAGAAT CCACGTAATA TCGTAGAAGC GGATCACTAT
     7681 CTGAGATTTC CAAAAGGGCA TCAAGCAACA TTTCTCTCTG TTCAGGTTTA CTCGGTTCAA
     7741 CAGTTGTTTG TAGTAGAGGG TGCGGATTTT CAATCTTTTT TCTCTGTGGC AATAGTTTTG
     7801 TATCTCCAAG AACACTATTT AACTTCAAAA ACTCATTTTG CAAAATAACA ATTTCTCCAG
     7861 AATAAGCTCT ATCAATCTTA CATAATTCAC CATTTATTGA AGTATACATT TCTGTAACTT
     7921 TTATTTTTTC TTTTTCTGAT ACTCTAACCG AATCTCGTAA ATGTAGTACT CCACTATAAA
     7981 GGCGTATATA TGCAAGACGT TGTCTTTTTT TTGTATATTC AATTTTGAAA ACATTTCCGC
     8041 AAAGTTCAGA CGGACCTCGA TGTGTTGATG AATAAAATTT ATTAGTAATA ACTTCTATAA
     8101 GGTTATCAAT CCCTATATTA CTTTTTGCAC TTCCATGATA AAGAGGGAAC AGAGAACAAT
     8161 TCTGAAATCT TATGCTTTCC TCTTGTTCGA GTTCCAATGC TTCTAATGAT TTACCGGACA
     8221 TATATTTCTC TAAAAGGTCA TCGTTTCCCT CTATTACCGT ATCCCATTGT TCAGATTCGG
     8281 TAAAGTTCGT CACACACACA TTAGGATACA GTTCTACCTT CTGTTTGATT ACAATTTCGG
     8341 CAGAAAGTTT CTCTTTAATA TCCTGATAAA CCGTTGATAA ATCAATTCCA TTTTGGTCAA
     8401 TCTTATTGAT AAAAAAGATT GTGGGAATCC CCATTTTCCT AAGTGCATGA AATAATATAC
     8461 GAGTTTGTGC TTGTACGCCA TCTTTTGCAG AAATCAGTAG AATTGCCCCA TCTAAAACTG
     8521 ATAATGAACG ATATACTTCT GCTAAGAAAT CCATATGTCC TGGCGTGTCT ATGATGTTCA
     8581 CCTTCGTATT TTCCCACTGA AAAGAGGTTA TTCCTGTCTG AATTGTAATT CCTCTCTGAC
     8641 GTTCTAAAAG CGTATTATCC GTCCTCGTTG TACCTTTGTC CACGCTTCCT AATTCTGTAA
     8701 TCGCTCCACT GTTATATAAT AAGCTTTCTG TTAAGGTAGT TTTTCCTGCA TCAACATGAG
     8761 CTAAAACTCC AATATTAATA ATTTTCATGT GATTTTCCTC CATTCAAAAG CCCAAAAGGG
     8821 CATAAAAATC CCAGTGATAA ATACTCTTAT CACTGGGATT TTTATGCATA ACCATAGGCA
     8881 TACAAAGCAT ACAGATATTC TCCGGATACT TTAGAATCAC ATGATAAAGG TATTCTTAAA
     8941 CTGGGTACAA AAAACTAAGC CCTCCTAAAA AAGGACATCC AATTATTTGT TCCCGCTATC
     9001 AAATTGACAG TTTATTTAAG AATACCTTGC CGCATATTTA TTAACTCCTT TTAAATAGAT
     9061 ACTTAAATTA TAGCACGTAA GAGCATATTT GTAAAGGAAT CTCCAATTTT TTATCAAAAA
     9121 GAGTACATGA TTACAAAGTA TCTGTAATCA TGTACCAATA TTTGTTATTT TACAATCTTC
     9181 CAATTATCTT GTTTTTCTAA TATCAACTCA AATTGCGAGA TTTGGGTTGC CTTTGTTTCT
     9241 TGATCTAAAT ATTTCACAGC GACATTCACG ATTACTTGG
//
