LOCUS       pMK11-parBp-mKate2_(pMK11-01)      9544 bp    DNA     circular UNA 
DEFINITION  Ligation of pJWV102-parBp-mKate2* into pMK11_v3*
FEATURES             Location/Qualifiers
     CDS             334..790
                     /label='czcD'
     promoter        854..859
                     /label=RBS
     CDS             866..2329
                     /cds_type=ORF
                     /note="Length: 1464"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
                     /label=parBp-mKate2
     promoter        866..868
                     /label=start
     CDS             866..1591
                     /label=ParB
     promoter        1445..1462
                     /label=parS??
     CDS             1592..1633
                     /label=linker
     CDS             1634..2325
                     /label=mKate2'
     promoter        2354..2442
                     /label=TER_rrnB
     promoter        2447..2498
                     /label=TER_rpsI
     promoter        2503..2530
                     /label=TER_tufA
     CDS             2551..2595
                     /note="promoterless and AUG-less 'lacZ"
                     /label='lacZ
     CDS             2660..3472
                     /note="end of pneumo ?-Gal"
                     /label='bgaA
     CDS             complement(4701..5562)
                     /note=?-lactamase
                     /label=bla
     CDS             5771..6706
                     /note="EII galactitol"
                     /label='gatC
     CDS             complement(7134..9068)
                     /note="tetracycline resistance"
                     /label=tetM
ORIGIN
        1 CTAGCGCTAT ATGCGTTGAT GCAATTTCTA TGCGCACCCG TTCTCGGAGC ACTGTCCGAC
       61 CGCTTTGGCC GCCGCCCAGT CCTGCTCGCT TCGCTACTTG GAGCCACTAT CGACTACGCG
      121 ATCATGGCGA CCACACCCGT CCTGTGGATC TATCGATGCA TGCAGCCGAC GGTACCGCAT
      181 GCTGCTGATA CGGCGGTCAA TGTTAGTCAT ATGGACACTT AAGGCAAATT GTTCAGAACT
      241 GAATAAAGCT GACGTTTTGC TTCTATCCTT TCTTTGAGTT TTAGTGGATA ATGATAATGA
      301 ACAAGGTGTT CATAAATCTA TTATAACAAA GGAATGAGAA ATATGAAGGC AAAATATGCT
      361 GTTTGGGTGG CTTTTTTCTT AAATTTGACT TATGCCATTG TTGAGTTTAT TGCAGGTGGA
      421 GTATTTGGTT CTAGCGCTGT TCTTGCTGAC TCTGTGCATG ACTTGGGAGA TGCGATTGCA
      481 ATTGGAATAT CAGCTTTTCT AGAAACAATC TCCAATCGTG AAGAAGACAA TCAGTACACC
      541 TTGGGCTATA AGCGGTTTAG CCTGCTAGGA GCCTTAGTAA CAGCTGTGAT TCTCGTAACG
      601 GGCTCTGTTC TAGTCATTTT GGAAAATGTC ACGAAGATTT TGCATCCGCA ACCAGTCAAT
      661 GATGAGGGGA TTCTCTGGTT AGGAATTATT GCGATTACTA TCAATCTGTT AACGAGTCTG
      721 GTGGTTGGTA AGGGAAAGAC AAAGAATGAG TCTATTCTGA GTCTGCGGAT CTTAAGGAGG
      781 CAAATATGAG AATTCCAATC CCGGGTAAAG CTTCCAGCGG CCGCATAGGA TCTCGAGTAC
      841 TGATTAACTA ATAAGGAGGA CAAACATGAG TAATAGTTTC GGATTTACAG ACCTTATGAA
      901 CAAGGACGAA CACAAGCGAA AAAAAACAAA TACAAAAAAT ATTCCTATTG AGGAGATAAA
      961 GGAAAATGAG AATAACAACT ATGATTTAGT TGACATTGAT AAGTTAGCTG ATAGCATAGA
     1021 CGAGTTAGGC TTATTACAAC CCGTTCTTGT TAAACAACGT GATAAATATA GCTATGAATT
     1081 GATTGCAGGG CATAGACGAT TTAATGCTAT TAAAAAACTA ATTAGTGAAA ATAGACTCCC
     1141 TGAAGATTAC GAAGTTCTCG CTAAGAAAGT TGATGAAGAC GAGGACGAGC TTGTAACAAG
     1201 ACTAAAGTTG CATGAAACTA ACCTACAAAC TCGCTCGCTC TTAAAAATGC CAGAAGAAGA
     1261 AAAAATAGCC ATCATTGATG ACTATATGGA TATTTTAGAT AAAGCCAAAA AACAAGGGGT
     1321 ACAAATCAAC GGAAAACCTG TTAAAGGAAA GACTAGGGAC TTAATTGCCG AAAGATTTGG
     1381 TATCAGTCAT TATACCGCTC AAAAACTGAT TAGAAAAGCT AAAGAACAAG GTGGTGAAGA
     1441 AGAGGGGGCT AAATTTAGCC CCCAAAAGAA AACCGCTAAA AAGCCAATCA CGCAGTTAAA
     1501 GAAAATTGAA ACTCAATTAG AAAAATTAGA GTTTGAGGGA ACGGAAGAAG AACAAGAAAT
     1561 CAAGAAAAAA TTGATTGAGT TGTTGATGAA ATCTAGAGGA TCTGGAGGAG AAGCTGCAGC
     1621 TAAAGGATCC TCATCAGAAC TTATCAAGGA AAATATGCAC ATGAAATTGT ACATGGAAGG
     1681 AACAGTAAAT AATCACCACT TTAAATGTAC CTCAGAAGGA GAAGGAAAAC CATATGAAGG
     1741 TACTCAAACC ATGCGTATTA AGGCCGTTGA AGGTGGACCA TTGCCTTTTG CCTTTGATAT
     1801 TCTTGCTACA TCTTTTATGT ACGGATCAAA GACTTTTATC AATCATACCC AAGGTATCCC
     1861 AGATTTCTTT AAACAGTCAT TTCCTGAAGG ATTTACATGG GAACGTGTCA CAACTTATGA
     1921 AGATGGTGGA GTATTGACAG CAACTCAAGA TACATCTTTG CAAGATGGTT GTCTTATCTA
     1981 CAATGTAAAG ATCCGTGGAG TTAATTTTCC AAGTAATGGT CCTGTTATGC AGAAAAAGAC
     2041 CCTTGGATGG GAAGCATCTA CCGAAACATT ATATCCTGCC GATGGTGGAT TGGAAGGTCG
     2101 TGCTGATATG GCATTGAAAC TTGTCGGTGG AGGTCACCTT ATCTGTAATT TGAAGACCAC
     2161 ATACCGTTCT AAAAAGCCAG CTAAGAATCT TAAGATGCCT GGTGTTTACT ACGTCGATCG
     2221 TCGTTTAGAA CGTATCAAAG AAGCAGATAA GGAAACTTAT GTTGAACAGC ACGAAGTAGC
     2281 CGTCGCACGT TATTGTGATT TGCCTAGTAA ATTGGGACAC CGTGACTAGT CAAGGTCGGC
     2341 AATTCTGCAG TACTAGGACG CCGCCAAGCC AGCTTAAACC CAGCTCAATG AGCTGGGTTT
     2401 TTTGTTTGTT AAAAATGAAG AAGAAACTGT GAAGCGTATT TATAGCAAAG CACTCAAAAG
     2461 TTTACCTTAT GGGTGCTTTT TTCGTGCTTT TTTGAAAAGA CAAAAAAAAG AACCTTGCCA
     2521 AGCAAGATTC TTTTGATAGC GCTATCGCTG AGCGCCGGTC GCTACCATTA CCAGTTGGTC
     2581 TGGTGTCAAA AATAATAATA ACCGGGCAGG CCATGTCTGC CCGTATTTCG CGTAAGGAAA
     2641 TCCATTATGT ACTATTTCTG GTGATGAAAT CAACGTAACA TTTAAAGCTG TCAAAGCCAA
     2701 AGTCATGAGA TGGCGTATGG AGCGTAAAGC TGACAAGAGC GGTGTTGCGA TGATTGAGAT
     2761 GACCTTCCTT GCACCAAGTG AATTGCCTCA AGAAAGCACT CAATCAAAGA TTCTTGTAGA
     2821 TGGAAAAGAA CTTGCTGATT TCGCTGAAAA TCGTCAAGAC TATCAAATTA CCTATAAAGG
     2881 TCAACGGCCA AAAGTCTCAG TTGAAGAAAA CAATCAAGTA GCTTCAACTG TGGTAGATAG
     2941 TGGAGAAGAT AGCCTTCCAG TACTTGTTCG CCTCGTTTCA GAAAGTGGAA AACAAGTCAA
     3001 GGAATACCGT ATCCAGTTGA CTAAGGAAAA ACCAGTTTCT GCTGTACAAG AAGATCTTCC
     3061 AAAACTCGAA TTTGTTGAAA AAGATTTGGC CTACAAGACA GTTGAGAAAA AAGATTCAAC
     3121 ACTGTATCTA GGTGAAACTC GTGTAGAACA AGAAGGAAAA GTTGGAAAAG AACGTATCTT
     3181 TACAGTGATT AATCCTGATG GAAGTAAGGA AGAAAAACTC CGTGAAGTGG TAGAAGTTCC
     3241 GACAGACCGC ATCGTCTTGG TTGGAACCAA ACCAGTAGCT CAAGAAGCTA AAAAACCACA
     3301 AGTGTCAGAA AAAGCAGATA CAAAACCAAT TGATTCAAGT GAAGCTGATC AAACTAATAA
     3361 AGCCCAGTTA CCAAATACAG GTAGTGCGGC AAGCCAAGCA GCAGTAGCAG CAGGTTTAGC
     3421 TCTTCTAGGT TTGAGTGCAG GATTAGTAGT TACTAAAGGT AAAAAAGAAG ACTAGCTGCC
     3481 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
     3541 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
     3601 TTGGCGGGTG TCGGGGCGCA GCCATGACCC AGTCACGTAG CGATAGCGGA GTGTATACTG
     3661 GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT
     3721 ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGC TCTTCCGCTT CCTCGCTCAC
     3781 TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT
     3841 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA
     3901 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC
     3961 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT
     4021 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
     4081 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG
     4141 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA
     4201 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA
     4261 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC
     4321 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
     4381 AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG
     4441 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA
     4501 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC
     4561 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG
     4621 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA
     4681 TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT
     4741 CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG
     4801 GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC
     4861 TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC
     4921 AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC
     4981 GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTGCA GGCATCGTGG TGTCACGCTC
     5041 GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC
     5101 CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA
     5161 GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT
     5221 GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA
     5281 GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAACA CGGGATAATA CCGCGCCACA
     5341 TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG
     5401 GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC
     5461 AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC
     5521 AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA
     5581 TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA
     5641 GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCTA
     5701 AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA GGCCCTTTCG
     5761 TCTTCAAGAA TTAATTCCTT CTTAACGCCC CAAGTTCATC ACCAATGACA TCAACTCACA
     5821 TGAACTACAT GATGAACCCA GTTATCATGG TTTTGGATAA GATTTTTGAA AAATTCTTCC
     5881 CAGGCCTTGA TAAATATGAC TTTGATGCTG CTAAATTGAA CAAGAAAATC GGTTTCTGGG
     5941 GATCTAAATT CTTCATCGGT TTCATCCTTG GTATCGTTAT CGGTATTATG GGAACTCCAC
     6001 ATCCAATTGC AGGTGTTGCA GATGCAGATA AATGGCGTCT TGTTATCAAA GGATGGTTGT
     6061 CTCTTGGTTT GACTGCCGGT GTATCTTTGG AACTCTTCTC ACTTATCGGT TCATGGTTCA
     6121 TCGCAGCCGT AGAACCACTA TCACAAGGTA TTACAAACGT TGCTACTAAA CGTCTTCAAG
     6181 GACGTAAATT CAATATCGGT CTTGACTGGC CATTCATCGC TGGTCGTGCT GAAATCTGGG
     6241 CTTGTGCCAA CGTACTTGCA CCAATCATGT TGATTGAAGC AGTGCTTCTT TCAAAAGTTG
     6301 GAAATGGTAT CTTGCCACTT GCAGGTATCA TCGCTATGGG TGTTACTCCA GCTCTCTTGG
     6361 TTGTAACTCG TGGTAAATTG CTCCGTATGA TTATCTTCGG AACACTCTTG TTGCCACTCT
     6421 TCCTTCTTTC AGGTACACTT ATTGCACCAT TTGCAACAGA ACTTGCTAAA GGTGTAGGTG
     6481 CCTTCCCAGA AGGTGTGAGC CAAACTCAAT TGATTACTCA CTCTACTCTT GAAGGACCAA
     6541 TCGAAAAACT TCTTGGTTGG ACAATTGGTA ACACTACAAC TGGTGATATC AAAGCAATCC
     6601 TTGGTGCAGT AGCCTTCCTT GTATTCTATA TCGGTATCTT TGCTTGGTAC AGAAAACAAA
     6661 TGATCAAACG TAACGAAGAG TACGCAGCAA AAGCAAAATA ATGCGCTCCG CTAGCTTTAC
     6721 AGACAAAGAA CTATCCTTAA TGGAAGCACT AGCAAAAGGT ATCAATGAAG CAAGAAATAT
     6781 TGAAGGCTAG TCAGTAAAAT TCAGATAAAC AAAAAGAGCC GATAAGATGA GAGTTTCATT
     6841 TCTCAAATTA TCGGCTCTGC GTCTTGGCGT CTGGCTCTTT GATTGTTATT ATATTCATTT
     6901 TTTGAACATT AATTAAAGTT TCGTTCTTAC ATTTGGGGCA GTATAAAGGG AAATTTTTAA
     6961 GTATAGTATC CACTCGTATT CGTAGTCTTG TTTTATTTCC ACAAATAGGA CACAATATCC
     7021 ACTTGTAGTT TATAATAACA ATCTCCTCCT TTCCACTTTA ATTCAAATCT ATATTAAAGA
     7081 ATATTTCATC TTATTTAATA AGAAACCATA TTTATATAAC AACATAAAAC GCACTAAGTT
     7141 ATTTTATTGA ACATATATCT TACTTTATCT ATCTGACTAT TTAGACGACG GGTCTGGCAA
     7201 ACAGGTTCGC CAGTGGTAAC CTGATATCCT TTTAGCTCTG CTAAACAAAC ACTAAGCCCA
     7261 TTTGTAAAAA AAGTTAAATC ATTGCGATAA TCTTGAATAC ATCGAGCAGG AATTTCTCCA
     7321 ATAATAATGA CCTCATTATT TTTCAGTTGA GTATTTACGA TATTTGCACA ATATTTGGGA
     7381 GCATCGTTAT ATGCCCGTGA AAGATATTCC TGTGGTGCAT AAACTTTAAA ACTAAGATAT
     7441 GGCTCTAACA ATTCTGTTCC AGCTTTTCTA AAGGCTTGCT CCAATACAAT AGGAGCAAGC
     7501 ATCCGAAAAT CTGCTGGGGT ACTAACAGGG CTATAGTATA AGCCATACTT AAAACAGATT
     7561 TTACAGTCCG TCACATTCCA ACCATACAAT CCTTGTTCAC AACCATAGCG TATCCCTTCC
     7621 ATAACTGCAT TTTGAAATGA TTGATTTAAG TATCCAAGAG AAACCGAGCT CTCATACTGC
     7681 ATTCCACTTC CCAACGGAAG CGGTGATACA GATAAACCAA TGGAAGCCCA GAAAGGATTT
     7741 GGCGGCACTT CGATGTGAAT GGTATATTCT GCATTTTTTA ACGGTCTCTC CATATAAATG
     7801 ACTGTAGGCT CTTTTAGTTC TATCTCCACA TGATACTTTT CTTGCAACAG TGCACTAATC
     7861 ACTTCCATTT GTACTTTCCC TAAGAAAGAA AGTATAATTT CATGTGTCGT AGAATCCACG
     7921 TAATATCGTA GAAGCGGATC ACTATCTGAG ATTTCCAAAA GGGCATCAAG CAACATTTCT
     7981 CTCTGTTCAG GTTTACTCGG TTCAACAGTT GTTTGTAGTA GAGGGTGCGG ATTTTCAATC
     8041 TTTTTTCTCT GTGGCAATAG TTTTGTATCT CCAAGAACAC TATTTAACTT CAAAAACTCA
     8101 TTTTGCAAAA TAACAATTTC TCCAGAATAA GCTCTATCAA TCTTACATAA TTCACCATTT
     8161 ATTGAAGTAT ACATTTCTGT AACTTTTATT TTTTCTTTTT CTGATACTCT AACCGAATCT
     8221 CGTAAATGTA GTACTCCACT ATAAAGGCGT ATATATGCAA GACGTTGTCT TTTTTTTGTA
     8281 TATTCAATTT TGAAAACATT TCCGCAAAGT TCAGACGGAC CTCGATGTGT TGATGAATAA
     8341 AATTTATTAG TAATAACTTC TATAAGGTTA TCAATCCCTA TATTACTTTT TGCACTTCCA
     8401 TGATAAAGAG GGAACAGAGA ACAATTCTGA AATCTTATGC TTTCCTCTTG TTCGAGTTCC
     8461 AATGCTTCTA ATGATTTACC GGACATATAT TTCTCTAAAA GGTCATCGTT TCCCTCTATT
     8521 ACCGTATCCC ATTGTTCAGA TTCGGTAAAG TTCGTCACAC ACACATTAGG ATACAGTTCT
     8581 ACCTTCTGTT TGATTACAAT TTCGGCAGAA AGTTTCTCTT TAATATCCTG ATAAACCGTT
     8641 GATAAATCAA TTCCATTTTG GTCAATCTTA TTGATAAAAA AGATTGTGGG AATCCCCATT
     8701 TTCCTAAGTG CATGAAATAA TATACGAGTT TGTGCTTGTA CGCCATCTTT TGCAGAAATC
     8761 AGTAGAATTG CCCCATCTAA AACTGATAAT GAACGATATA CTTCTGCTAA GAAATCCATA
     8821 TGTCCTGGCG TGTCTATGAT GTTCACCTTC GTATTTTCCC ACTGAAAAGA GGTTATTCCT
     8881 GTCTGAATTG TAATTCCTCT CTGACGTTCT AAAAGCGTAT TATCCGTCCT CGTTGTACCT
     8941 TTGTCCACGC TTCCTAATTC TGTAATCGCT CCACTGTTAT ATAATAAGCT TTCTGTTAAG
     9001 GTAGTTTTTC CTGCATCAAC ATGAGCTAAA ACTCCAATAT TAATAATTTT CATGTGATTT
     9061 TCCTCCATTC AAAAGCCCAA AAGGGCATAA AAATCCCAGT GATAAATACT CTTATCACTG
     9121 GGATTTTTAT GCATAACCAT AGGCATACAA AGCATACAGA TATTCTCCGG ATACTTTAGA
     9181 ATCACATGAT AAAGGTATTC TTAAACTGGG TACAAAAAAC TAAGCCCTCC TAAAAAAGGA
     9241 CATCCAATTA TTTGTTCCCG CTATCAAATT GACAGTTTAT TTAAGAATAC CTTGCCGCAT
     9301 ATTTATTAAC TCCTTTTAAA TAGATACTTA AATTATAGCA CGTAAGAGCA TATTTGTAAA
     9361 GGAATCTCCA ATTTTTTATC AAAAAGAGTA CATGATTACA AAGTATCTGT AATCATGTAC
     9421 CAATATTTGT TATTTTACAA TCTTCCAATT ATCTTGTTTT TCTAATATCA ACTCAAATTG
     9481 CGAGATTTGG GTTGCCTTTG TTTCTTGATC TAAATATTTC ACAGCGACAT TCACGATTAC
     9541 TTGG
//
