LOCUS       pcDNA3.1(+)	5428 bp	DNA	SYN	19-Jan-2012
DEFINITION  pcDNA3.1(+)
ACCESSION    
KEYWORDS    
SOURCE    
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..7164
                     /dnas_title="5"
                     /organism="pcDNA3.1(+)"
                     /mol_type="other DNA"
     misc_feature    complement(2758..2775)
                     /label=BGH_rev_primer
     terminator      2761..2988
                     /label=bGH_PA_terminator
     rep_origin      3051..3357
                     /label=f1_origin
     misc_feature    complement(3471..3491)
                     /label=pBABE_3_primer
     misc_feature    complement(3477..3692)
                     /label=SV40_enhancer
     promoter        3489..3757
                     /label=SV40_promoter
     rep_origin      3656..3733
                     /label=SV40_origin
     misc_feature    3718..3737
                     /label=SV40pro_F_primer
     CDS             3872..4666
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
                     /note="ORF frame 3"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            3875..4663
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
     terminator      4843..4962
                     /label=SV40_PA_terminator
     misc_feature    4931..4950
                     /label=EBV_rev_primer
     misc_feature    complement(5006..5024)
                     /label=M13_reverse_primer
     misc_feature    complement(5023..5045)
                     /label=M13_pUC_rev_primer
     promoter        complement(5059..5088)
                     /label=lac_promoter
     rep_origin      complement(5397..6013)
                     /label=pBR322_origin
     CDS             complement(6168..7028)
                     /label=Ampicillin
                     /gene="Ampicillin"
                     /note="ORF frame 1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     gene            complement(6168..7028)
                     /label=Ampicillin
                     /gene="Ampicillin"
     promoter        complement(7070..7098)
                     /label=AmpR_promoter
     misc_feature    82..369
                     /label=CAG_enhancer
     promoter        17..619
                     /label=CMV_immearly_promoter
     misc_feature    536..556
                     /label=CMV_fwd_primer
     promoter        630..648
                     /label=T7_promoter
     misc_feature    678..711
                     /note="LoxP3"
     misc_feature    1861..2450
                     /note="WPRE"
     misc_feature    complement(2477..2705)
                     /note="SV40-pA"
     PCR_primer      complement(2690..2712)
                     /pair=""
                     /primer="CACGCCTTAAGATACATTGATGA"
                     /current=1
     promoter        673..677
                     /label=CMV_immearly_promoter
     misc_feature    853..879
                     /note="HA"
     misc_feature    919..945
                     /note="HA"
     misc_feature    886..912
                     /note="HA"
     misc_feature    853..954
                     /note="3X HA Tag"
     PCR_primer      724..743
                     /pair=""
                     /primer="ATGGGTTGCTGTTTCTCCAA"
                     /current=0
     misc_feature    724..852
                     /note="Membrane Tag plus spacer PERFECT"
     CDS             724..852
                     /label=EGFP
                     /gene="EGFP"
                     /note="ORF frame 1"
                     /translation="MGCCFSKTAAKGEAAAERPGEAAVASSPSKANGQENGHVKVGSV
                     SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPT
                     LVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGD
                     TLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGS
                     VQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGM
                     DELYK*"
     CDS             955..1737
                     /label=ORF frame 3
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYD
                     GTNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTM
                     TFEDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDG
                     VLKGDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTV
                     YESAVARNSTDGMDELYKSARVSKLNPPDESGPGCMSCKCVLS"
     misc_feature    1747..1809
                     /note="2A Peptide"
     misc_feature    670..1870
                     /note="SEQUENCED"
ORIGIN      
        1 GACGGATCGG GAGATCCTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC
       61 CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC
      121 AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG
      181 ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT
      241 CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC
      301 TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA
      361 TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG
      421 CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT
      481 TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA
      541 ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCTCTG GCTAACTAGA
      601 GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC ACTATAGGGA GACCCAAGCT
      661 GGCTAGCaat tACTAGTATA ACTTCGTATA GCATACATTA TACGAAGTTA TcGGCGCGCC
      721 ACCATGGGTT GCTGTTTCTC CAAGACCGCA GCGAAGGGAG AAGCCGCCGC GGAGAGGCCT
      781 GGGGAGGCGG CTGTGGCCTC GTCGCCTTCC AAAGCGAACG GACAGGAGAA TGGCCACGTG
      841 AAGGTAGGAT CCTACCCATA CGATGTTCCT GACTATGCGG CCgaaTATCC CTATGACGTC
      901 CCGGACTATG CAGCCgaaTA TCCATAcGAC GTTCCAGATT ACGCTGCCaa acttatggtg
      961 agcaagggcg aggagaccac aatgggcgta atcaagcccg acatgaagat caagctgaag
     1021 atggagggca acgtgaatgg ccacgccttc gtgatcgagg gcgagggcga gggcaagccc
     1081 tacgacggca ccaacaccat caacctggag gtgaaggagg gagcccccct gcccttctcc
     1141 tacgacattc tgaccaccgc gttcgcctac ggcaacaggg ccttcaccaa gtaccccgac
     1201 gacatcccca actacttcaa gcagtccttc cccgagggct actcttggga gcgcaccatg
     1261 accttcgagg acaagggcat cgtgaaggtg aagtccgaca tctccatgga ggaggactcc
     1321 ttcatctacg agatacacct caagggcgag aacttccccc ccaacggccc cgtgatgcag
     1381 aagaagacca ccggctggga cgcctccacc gagaggatgt acgtgcgcga cggcgtgctg
     1441 aagggcgacg tcaagcacaa gctgctgctg gagggcggcg gccaccaccg cgttgacttc
     1501 aagaccatct acagggccaa gaaggcggtg aagctgcccg actatcactt tgtggaccac
     1561 cgcatcgaga tcctgaacca cgacaaggac tacaacaagg tgaccgttta cgagagcgcc
     1621 gtggcccgca actccaccga cggcatggac gagctgtata agAGTGCACG CGTGAGTAAG
     1681 CTGAACCCTC CTGATGAGAG TGGCCCCGGC TGCATGAGCT GCAAGTGTGT GCTCTCCctG
     1741 TACAAGGGCA GTGGAGAGGG CAGAGGAAGT CTGCTAACAT GCGGTGACGT CGAGGAGAAT
     1801 CCTGGCCCAG AATTCTGCAG ATATCCAGCA CAGTGGCGGC CGCTCGAGgg CGCGCCccgc
     1861 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     1921 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     1981 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     2041 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     2101 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     2161 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     2221 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     2281 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     2341 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     2401 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
     2461 tcgagactgc tgcaccagat cataatcagc cataccacat ttgtagaggt tttacttgct
     2521 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     2581 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     2641 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     2701 tcttaaggcg tgGCGGCCGC ATCGAGTCTA GAGGGCCCGT TTAAACCCGC TGATCAGCCT
     2761 CGACTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA
     2821 CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT
     2881 GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG
     2941 ATTGGGAAGA CAATAGCAGG CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG
     3001 AAAGAACCAG CTGGGGCTCT AGGGGGTATC CCCACGCGCC CTGTAGCGGC GCATTAAGCG
     3061 CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG
     3121 CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC
     3181 TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA
     3241 AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC
     3301 CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC
     3361 TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT
     3421 GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTAATTCTGT GGAATGTGTG
     3481 TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA
     3541 TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT
     3601 GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC
     3661 GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT
     3721 TTATGCAGAG GCCGAGGCCG CCTCTGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT
     3781 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC
     3841 TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG
     3901 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG
     3961 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA
     4021 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT
     4081 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA
     4141 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC
     4201 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC
     4261 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC
     4321 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT
     4381 GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA
     4441 TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG
     4501 CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA
     4561 AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA
     4621 TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG
     4681 TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC
     4741 GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC
     4801 CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT
     4861 AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG
     4921 CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG
     4981 ACCTCTAGCT AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT
     5041 CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC
     5101 TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA
     5161 AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT
     5221 ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG
     5281 CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC
     5341 GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG
     5401 TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA
     5461 AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC
     5521 TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC
     5581 CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG
     5641 GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC
     5701 TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA
     5761 GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG
     5821 AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG
     5881 AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT
     5941 GGTAGCGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA
     6001 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG
     6061 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA
     6121 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA
     6181 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC
     6241 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG
     6301 ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA
     6361 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT
     6421 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT
     6481 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC
     6541 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC
     6601 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA
     6661 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG
     6721 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG
     6781 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA
     6841 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA
     6901 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA
     6961 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA
     7021 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG
     7081 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT
     7141 CCCCGAAAAG TGCCACCTGA CGTC
//

