LOCUS       pcDNA3.1(+)	5428 bp	DNA	SYN	19-Jan-2012
DEFINITION  pcDNA3.1(+)
ACCESSION    
KEYWORDS    
SOURCE    
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..7703
                     /dnas_title="pcDNA3.1 Entry BglII-FRT2-KpnI-
                     AscI-Mb2Tomato-2A-EcoRI-XhoI-AscI -WPRE-Sv40pA-SpeI L60"
                     /organism="pcDNA3.1(+)"
                     /mol_type="other DNA"
     misc_feature    complement(3297..3314)
                     /label=BGH_rev_primer
     terminator      3300..3527
                     /label=bGH_PA_terminator
     rep_origin      3590..3896
                     /label=f1_origin
     misc_feature    complement(4010..4030)
                     /label=pBABE_3_primer
     misc_feature    complement(4016..4231)
                     /label=SV40_enhancer
     promoter        4028..4296
                     /label=SV40_promoter
     rep_origin      4195..4272
                     /label=SV40_origin
     misc_feature    4257..4276
                     /label=SV40pro_F_primer
     CDS             4411..5205
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
                     /note="ORF frame 3"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            4414..5202
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
     terminator      5382..5501
                     /label=SV40_PA_terminator
     misc_feature    5470..5489
                     /label=EBV_rev_primer
     misc_feature    complement(5545..5563)
                     /label=M13_reverse_primer
     misc_feature    complement(5562..5584)
                     /label=M13_pUC_rev_primer
     promoter        complement(5598..5627)
                     /label=lac_promoter
     rep_origin      complement(5936..6552)
                     /label=pBR322_origin
     CDS             complement(6707..7567)
                     /label=Ampicillin
                     /gene="Ampicillin"
                     /note="ORF frame 1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     gene            complement(6707..7567)
                     /label=Ampicillin
                     /gene="Ampicillin"
     promoter        complement(7609..7637)
                     /label=AmpR_promoter
     misc_feature    82..369
                     /label=CAG_enhancer
     promoter        17..619
                     /label=CMV_immearly_promoter
     misc_feature    536..556
                     /label=CMV_fwd_primer
     promoter        630..648
                     /label=T7_promoter
     misc_feature    2402..2991
                     /note="WPRE"
     PCR_primer      complement(3231..3253)
                     /pair=""
                     /primer="CACGCCTTAAGATACATTGATGA"
                     /current=1
     misc_feature    complement(3018..3246)
                     /note="SV40-pA"
     misc_feature    678..684
                     /note=""
     misc_feature    705..711
                     /note=""
     misc_feature    678..711
                     /note="FRT2 (5T2)"
     misc_feature    682..1530
                     /dnas_title="SEQUENCED"
                     /note="SEQUENCED"
     CDS             730..858
                     /label=EGFP
                     /gene="EGFP"
                     /note="ORF frame 1"
                     /translation="MGCCFSKTAAKGEAAAERPGEAAVASSPSKANGQENGHVKVGSV
                     SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPT
                     LVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGD
                     TLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGS
                     VQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGM
                     DELYK*"
     PCR_primer      730..749
                     /pair=""
                     /primer="ATGGGTTGCTGTTTCTCCAA"
                     /current=0
     misc_feature    730..858
                     /note="Mb tag sequence plus linker from mT/mG mice"
     PCR_primer      859..878
                     /pair=""
                     /primer="GTGAGCAAGGGAGAGGAGGT"
                     /current=1
     misc_feature    859..2283
                     /note="TdTomato"
     misc_feature    2284..2346
                     /note="2A Peptide"
ORIGIN      
        1 GACGGATCGG GAGATCCTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC
       61 CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC
      121 AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG
      181 ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT
      241 CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC
      301 TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA
      361 TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG
      421 CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT
      481 TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA
      541 ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCTCTG GCTAACTAGA
      601 GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC ACTATAGGGA GACCCAAGCT
      661 GGCTAGCaat tAGATCTGAA GTTCCTATTC tctagaagGT ATAGGAACTT CcGGTACCGG
      721 CGCGCCACCA TGGGTTGCTG TTTCTCCAAG ACCGCAGCGA AGGGAGAAGC CGCCGCGGAG
      781 AGGCCTGGGG AGGCGGCTGT GGCCTCGTCG CCTTCCAAAG CGAACGGACA GGAGAATGGC
      841 CACGTGAAGG TAGGATCCGT GAGCAAGGGA GAGGAGGTCA TCAAAGAGTT CATGCGCTTC
      901 AAGGTGCGCA TGGAGGGCTC CATGAACGGC CACGAGTTCG AGATCGAGGG CGAGGGCGAG
      961 GGCCGCCCCT ACGAGGGCAC CCAGACCGCC AAGCTGAAGG TGACCAAGGG CGGCCCCCTG
     1021 CCCTTCGCCT GGGACATCCT GTCCCCCCAG TTCATGTACG GCTCCAAGGC GTACGTGAAG
     1081 CACCCCGCCG ACATCCCCGA TTACAAGAAG CTGTCCTTCC CCGAGGGCTT CAAGTGGGAG
     1141 CGCGTGATGA ACTTCGAGGA CGGCGGTCTG GTGACCGTGA CCCAGGACTC CTCCCTGCAG
     1201 GACGGCACGC TGATCTACAA GGTGAAGATG CGCGGCACCA ACTTCCCCCC CGACGGCCCC
     1261 GTAATGCAGA AGAAGACCAT GGGCTGGGAG GCCTCCACCG AGCGCCTGTA CCCCCGCGAC
     1321 GGCGTGCTGA AGGGCGAGAT CCACCAGGCC CTGAAGCTGA AGGACGGCGG CCACTACCTG
     1381 GTGGAGTTCA AGACCATCTA CATGGCCAAG AAGCCCGTGC AACTGCCCGG CTACTACTAC
     1441 GTGGACACCA AGCTGGACAT CACCTCCCAC AACGAGGACT ACACCATCGT GGAACAGTAC
     1501 GAGCGCTCCG AGGGCCGCCA CCACCTGTTC CTGGGGCATG GCACCGGCAG CACCGGCAGC
     1561 GGCAGCTCCG GCACCGCCTC CTCCGAGGAC AACAACATGG CCGTCATCAA AGAGTTCATG
     1621 CGCTTCAAGG TGCGCATGGA GGGCTCCATG AACGGCCACG AGTTCGAGAT CGAGGGCGAG
     1681 GGCGAGGGCC GCCCCTACGA GGGCACCCAG ACCGCCAAGC TGAAGGTGAC CAAGGGCGGC
     1741 CCCCTGCCCT TCGCCTGGGA CATCCTGTCC CCCCAGTTCA TGTACGGCTC CAAGGCGTAC
     1801 GTGAAGCACC CCGCCGACAT CCCCGATTAC AAGAAGCTGT CCTTCCCCGA GGGCTTCAAG
     1861 TGGGAGCGCG TGATGAACTT CGAGGACGGC GGTCTGGTGA CCGTGACCCA GGACTCCTCC
     1921 CTGCAGGACG GCACGCTGAT CTACAAGGTG AAGATGCGCG GCACCAACTT CCCCCCCGAC
     1981 GGCCCCGTAA TGCAGAAGAA GACCATGGGC TGGGAGGCCT CCACCGAGCG CCTGTACCCC
     2041 CGCGACGGCG TGCTGAAGGG CGAGATCCAC CAGGCCCTGA AGCTGAAGGA CGGCGGCCGC
     2101 TACCTGGTGG AGTTCAAGAC CATCTACATG GCCAAGAAGC CCGTGCAACT GCCCGGCTAC
     2161 TACTACGTGG ACACCAAGCT GGACATCACC TCCCACAACG AGGACTACAC CATCGTGGAA
     2221 CAGTACGAGC GCTCCGAGGG CCGCCACCAC CTGTTCCTGT ACGGCATGGA CGAGCTGTAC
     2281 AAGGGCAGTG GAGAGGGCAG AGGAAGTCTG CTAACATGCG GTGACGTCGA GGAGAATCCT
     2341 GGCCCAGAAT TCTGCAGATA TCCAGCACAG TGGCGGCCGC TCGAGTCTAG gCGCGCCccg
     2401 ctaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     2461 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     2521 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     2581 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     2641 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     2701 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     2761 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     2821 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     2881 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     2941 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
     3001 gtcgagactg ctgcaccaga tcataatcag ccataccaca tttgtagagg ttttacttgc
     3061 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     3121 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     3181 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     3241 atcttaaggc gtgACTAGTA TCGAGTCTAG AGGGCCCGTT TAAACCCGCT GATCAGCCTC
     3301 GACTGTGCCT TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC
     3361 CCTGGAAGGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG
     3421 TCTGAGTAGG TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA
     3481 TTGGGAAGAC AATAGCAGGC ATGCTGGGGA TGCGGTGGGC TCTATGGCTT CTGAGGCGGA
     3541 AAGAACCAGC TGGGGCTCTA GGGGGTATCC CCACGCGCCC TGTAGCGGCG CATTAAGCGC
     3601 GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC
     3661 TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT
     3721 AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA
     3781 ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC
     3841 TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT
     3901 CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG
     3961 GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TAATTCTGTG GAATGTGTGT
     4021 CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT
     4081 CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG
     4141 CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG
     4201 CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT
     4261 TATGCAGAGG CCGAGGCCGC CTCTGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT
     4321 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT
     4381 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT
     4441 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC
     4501 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG
     4561 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG
     4621 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC
     4681 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC
     4741 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC
     4801 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC
     4861 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG
     4921 TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT
     4981 GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC
     5041 CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA
     5101 GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT
     5161 TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT
     5221 TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG
     5281 CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC
     5341 AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA
     5401 ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC
     5461 ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA
     5521 CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC
     5581 CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT
     5641 AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA
     5701 ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA
     5761 TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC
     5821 GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG
     5881 CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT
     5941 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA
     6001 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT
     6061 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC
     6121 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG
     6181 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT
     6241 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG
     6301 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA
     6361 AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA
     6421 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG
     6481 GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG
     6541 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA
     6601 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA
     6661 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA
     6721 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC
     6781 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA
     6841 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA
     6901 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT
     6961 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG
     7021 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC
     7081 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG
     7141 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG
     7201 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT
     7261 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT
     7321 CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC
     7381 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC
     7441 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG
     7501 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA
     7561 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA
     7621 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC
     7681 CCCGAAAAGT GCCACCTGAC GTC
//

