LOCUS       Exported                6140 bp ds-DNA     circular SYN 30-AUG-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 30, 2017 from SnapGene 4.0.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6140
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..6140
                     /label=Untitled
                     /note="                                                    
                                "
     misc_feature    1..384
                     /label=pUC18
                     /note="pUC18"
     misc_feature    1
                     /label=pUC18
                     /note="pUC18"
     primer_bind     352..375
                     /label=Primer 3
     primer_bind     364..386
                     /label=Primer 1
     primer_bind     393..411
                     /label=Primer 8
     misc_feature    411..728
                     /label=TRE TIGHT
                     /note="TRE TIGHT"
     CDS             478..738
                     /codon_start=1
                     /label=ORF frame 1
                     /translation="MSSLLPISDRERMSSLLPISDRERMSSLLPISDRERMSSLSLSVI
                     ENVCRVYSLSVIENVCRGRRVRWEAYISRARLVNRQIAWRRI"
     CDS             697..716
                     /codon_start=1
                     /label=pCEP_fwd_primer
                     /translation="RARLVN"
     CDS             699..723
                     /codon_start=1
                     /label=LNCX_primer
                     /translation="SSFSEPSD"
     primer_bind     complement(719..738)
                     /label=Primer 10
     primer_bind     745..767
                     /label=Primer 9
     misc_feature    complement(763..796)
                     /label=loxP 1
     misc_feature    complement(804..837)
                     /label=loxP 2
     misc_feature    846..879
                     /label=loxP 3
     CDS             899..922
                     /codon_start=1
                     /label=ORF frame 1
                     /translation="MVSVIKPE"
     misc_feature    935..961
                     /label=NLS
                     /note="NLS"
     misc_feature    962..1666
                     /label=PhiYFP / Y65A
                     /note="PhiYFP / Y65A"
     misc_feature    complement(1136..1174)
                     /label=PhiYFP/Y65A_Rev1
                     /note="PhiYFP/Y65A_Rev1"
     misc_feature    1145..1183
                     /label=PhiYFP/Y65A_For1
                     /note="PhiYFP/Y65A_For1"
     misc_feature    1220..1249
                     /label=PhiYFP_ScreenFor
                     /note="PhiYFP_ScreenFor"
     misc_feature    complement(1523..1552)
                     /label=PhiYFP_ScreenRev
                     /note="PhiYFP_ScreenRev"
     misc_feature    1742..1975
                     /label=SV40pA
                     /note="SV40pA"
     misc_feature    1881..1885
                     /label=pA
                     /note="pA"
     misc_feature    1910..1914
                     /label=pA
                     /note="pA"
     misc_feature    1918..1930
                     /label=mRNA ends
                     /note="mRNA ends"
     misc_feature    complement(1925..1947)
                     /label=eYFP-pA rev
                     /note="eYFP-pA rev"
     primer_bind     complement(1951..1975)
                     /label=Primer 5
     primer_bind     2012..2030
                     /label=Primer 6
     misc_feature    2034..2068
                     /label=FRT
                     /note="FRT"
     misc_feature    complement(2166..2189)
                     /label=MSCV_rev_primer
     misc_feature    2243..2640
                     /label=PGK Promoter
                     /note="PGK Promoter"
     misc_feature    2563..2582
                     /label=mPGK_F_primer
     misc_feature    2652..2705
                     /label=EM7 Promoter
                     /note="EM7 Promoter"
     CDS             2708..3511
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="ORF frame 3"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            2708..3508
                     /label=NeoR/KanR
     misc_feature    complement(3543..3560)
                     /label=BGH_rev_primer
     terminator      3546..3773
                     /label=bGH_PA_terminator
     misc_feature    3830..3864
                     /label=FRT POINT MUTATED - NOT FUNCTIONAL
                     /note="FRT POINT MUTATED - NOT FUNCTIONAL"
     primer_bind     complement(3876..3896)
                     /label=Primer 7
     misc_feature    3907..6140
                     /label=pUC18
                     /note="pUC18"
     primer_bind     complement(3931..3954)
                     /label=Primer 4
     primer_bind     complement(3932..3954)
                     /label=Primer 2
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgccgg cgggcccttt cgtcttcact cgagtttact
      421 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgatg
      481 tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
      541 agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttatcc
      601 ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt
      661 cgaggtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag
      721 atcgcctgga gaaggatcaa gcttggcaaa gaattcctcg agataacttc gtatagtata
      781 ccttatacga agttatcgag ctcataactt cgtataggat actttatacg aagttataac
      841 tcgagataac ttcgtatagc atacattata cgaagttata ccggcagatc tcgccaccat
      901 ggtgagtgtg attaaaccag aggctagctt caccatggct cctaagaaga agaggaaggt
      961 gatgagcagc ggcgccctgc tgttccacgg caagatcccc tacgtggtgg agatggaggg
     1021 caatgtggat ggccacacct tcagcatccg cggcaagggc tacggcgatg ccagcgtggg
     1081 caaggtggat gcccagttca tctgcaccac aggcgatgtg cccgtgccct ggagcaccct
     1141 ggtgaccacc ctgaccgcag gcgcccagtg cttcgccaag tacggccccg agctgaagga
     1201 tttctacaag agctgcatgc ccgatggcta cgtgcaggag cgcaccatca ccttcgaggg
     1261 tgatggcaat ttcaagaccc gcgccgaggt gaccttcgag aatggcagcg tgtacaatcg
     1321 cgtgaagctg aatggccagg gcttcaagaa ggatggccac gtgctgggca agaatctgga
     1381 gttcaatttc accccccact gcctgtacat ctggggcgat caggccaatc acggcctgaa
     1441 gagcgccttc aagatctgcc acgagatcac cggcagcaag ggcgatttca tcgtggccga
     1501 tcacacccag atgaataccc ccatcggcgg cggccccgtg cacgtgcccg agtaccacca
     1561 catgagctac cacgtgaagc tgagcaagga tgtgaccgat caccgcgata atatgagcct
     1621 gaaggagacc gtgcgcgccg tggattgccg caagacctac ctgtgacgct taattagctg
     1681 agcttggact cctgttgata gatccagtaa tgacctcaga actccatctg gccgcgactc
     1741 tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     1801 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     1861 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     1921 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta aggcgaagct
     1981 tcagccctat cattcggcgc gccgcggccg cagcataggg atccccgaat tccgaagttc
     2041 ctattctcta gaaagtatag gaacttcagg tctgaagagg agtttacgtc cagccaagct
     2101 agcttggctg caggtcgtcg aaattctacc gggtagggga ggcgcttttc ccaaggcagt
     2161 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
     2221 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
     2281 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
     2341 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
     2401 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
     2461 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc
     2521 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa
     2581 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc
     2641 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     2701 ctaaaccatg ggatcggcca ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     2761 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     2821 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     2881 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     2941 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     3001 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     3061 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     3121 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     3181 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     3241 ggcgcgcatg cccgacggcg atgatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     3301 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     3361 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     3421 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     3481 cttctatcgc cttcttgacg agttcttctg aggggatcaa ttctctagag ctcgctgatc
     3541 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     3601 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     3661 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     3721 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     3781 ggcggaaaga accagctggg gctcgactag agcttgcgga acccttcgcg aagttcatat
     3841 tctctagaaa gtataggaac ttcatcagtc aggtacataa tataacttcg ggcgcgttaa
     3901 ttaagaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     3961 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     4021 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     4081 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     4141 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     4201 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     4261 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4321 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4381 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4441 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4501 cgtggcgctt tctcaaagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4561 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4621 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4681 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4741 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     4801 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     4861 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     4921 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     4981 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     5041 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     5101 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     5161 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     5221 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     5281 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     5341 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     5401 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     5461 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     5521 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     5581 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     5641 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     5701 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     5761 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     5821 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     5881 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     5941 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     6001 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     6061 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     6121 tatcacgagg ccctttcgtc
//
