LOCUS       Exported                7455 bp ds-DNA     circular SYN 30-AUG-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 30, 2017 from SnapGene 4.0.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7455
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..7455
                     /label=Untitled
                     /note="                                                    
                                "
     misc_feature    1..384
                     /label=pUC18
                     /note="pUC18"
     misc_feature    1
                     /label=pUC18
                     /note="pUC18"
     primer_bind     217..236
                     /label=Primer 9
     misc_feature    260..781
                     /label=Sequenced p1655
                     /note="Sequenced p1655"
     misc_feature    279..575
                     /label=Sequenced
                     /note="Sequenced"
     primer_bind     352..375
                     /label=Primer 3
     primer_bind     364..386
                     /label=Primer 1
     misc_feature    387..565
                     /label=4xnrUAS
     promoter        387..407
                     /label=Gal10(2) UAS(1)
     promoter        418..434
                     /label=Gal10(2) UAS
     promoter        445..461
                     /label=synthetic near consensus UAS
     misc_feature    465
                     /label=A-G change from Halpern sequence
     promoter        472..488
                     /label=near consensus UAS
     misc_feature    513..571
                     /label=E1B promoter
     promoter        513..523
                     /label=adenovirus E1B TATA
     5'UTR           534..565
                     /label=carp beta-actin trx start
     primer_bind     complement(561..582)
                     /label=Primer 7
     misc_feature    complement(573..582)
                     /label=AttB2
     primer_bind     588..610
                     /label=Primer 6
     misc_feature    complement(606..639)
                     /label=loxP 1
     misc_feature    complement(647..680)
                     /label=loxP 2
     misc_feature    689..722
                     /label=loxP 3
     CDS             742..762
                     /codon_start=1
                     /label=ORF frame 1
                     /translation="MVSVITE"
     misc_feature    775..801
                     /label=NLS
                     /note="NLS"
     misc_feature    802..1506
                     /label=PhiYFP / Y65A
                     /note="PhiYFP / Y65A"
     misc_feature    complement(976..1014)
                     /label=PhiYFP/Y65A_Rev1
                     /note="PhiYFP/Y65A_Rev1"
     misc_feature    985..1023
                     /label=PhiYFP/Y65A_For1
                     /note="PhiYFP/Y65A_For1"
     misc_feature    1060..1089
                     /label=PhiYFP_ScreenFor
                     /note="PhiYFP_ScreenFor"
     misc_feature    complement(1363..1392)
                     /label=PhiYFP_ScreenRev
                     /note="PhiYFP_ScreenRev"
     misc_feature    1582..1815
                     /label=SV40pA
                     /note="SV40pA"
     misc_feature    1721..1725
                     /label=pA
                     /note="pA"
     misc_feature    1750..1754
                     /label=pA
                     /note="pA"
     misc_feature    1758..1770
                     /label=mRNA ends
                     /note="mRNA ends"
     misc_feature    complement(1765..1787)
                     /label=eYFP-pA rev
                     /note="eYFP-pA rev"
     primer_bind     complement(1791..1815)
                     /label=Primer 5
     misc_feature    1874..1907
                     /label=FRT
                     /note="FRT"
     misc_feature    complement(2006..2029)
                     /label=MSCV_rev_primer
     misc_feature    2403..2422
                     /label=mPGK_F_primer
     CDS             2548..3351
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="ORF frame 3"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            2548..3348
                     /label=NeoR/KanR
     misc_feature    complement(3383..3400)
                     /label=BGH_rev_primer
     terminator      3386..3613
                     /label=bGH_PA_terminator
     misc_feature    3671..3704
                     /label=FRT
                     /note="FRT"
     misc_feature    3712..5211
                     /label=Cdh5 homology
                     /note="Cdh5 homology"
     primer_bind     complement(5192..5211)
                     /label=Primer 8
     misc_feature    5222..7455
                     /label=pUC18
                     /note="pUC18"
     primer_bind     complement(5246..5269)
                     /label=Primer 4
     primer_bind     complement(5247..5269)
                     /label=Primer 2
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgccgg cggtggcttc taatccgtga gtcctagcgg
      421 gtgacagccc tccgtcttca caggcggagg agagtcttcc gtagggttcc tcggagtact
      481 gtcctccgac gcgtgcaagg gtcgactcta gagggtatat aatggatccc atcgcgtctc
      541 agcctcactt tgagctcctc cacacgaatt cgccactttg taagcttggc aaagaattcc
      601 tcgagataac ttcgtatagt ataccttata cgaagttatc gagctcataa cttcgtatag
      661 gatactttat acgaagttat aactcgagat aacttcgtat agcatacatt atacgaagtt
      721 ataccggcag atctcgccac catggtgagt gtgattacag aggctagctt caccatggct
      781 cctaagaaga agaggaaggt gatgagcagc ggcgccctgc tgttccacgg caagatcccc
      841 tacgtggtgg agatggaggg caatgtggat ggccacacct tcagcatccg cggcaagggc
      901 tacggcgatg ccagcgtggg caaggtggat gcccagttca tctgcaccac aggcgatgtg
      961 cccgtgccct ggagcaccct ggtgaccacc ctgaccgcag gcgcccagtg cttcgccaag
     1021 tacggccccg agctgaagga tttctacaag agctgcatgc ccgatggcta cgtgcaggag
     1081 cgcaccatca ccttcgaggg tgatggcaat ttcaagaccc gcgccgaggt gaccttcgag
     1141 aatggcagcg tgtacaatcg cgtgaagctg aatggccagg gcttcaagaa ggatggccac
     1201 gtgctgggca agaatctgga gttcaatttc accccccact gcctgtacat ctggggcgat
     1261 caggccaatc acggcctgaa gagcgccttc aagatctgcc acgagatcac cggcagcaag
     1321 ggcgatttca tcgtggccga tcacacccag atgaataccc ccatcggcgg cggccccgtg
     1381 cacgtgcccg agtaccacca catgagctac cacgtgaagc tgagcaagga tgtgaccgat
     1441 caccgcgata atatgagcct gaaggagacc gtgcgcgccg tggattgccg caagacctac
     1501 ctgtgacgct taattagctg agcttggact cctgttgata gatccagtaa tgacctcaga
     1561 actccatctg gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac
     1621 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     1681 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     1741 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     1801 atgtatctta aggcgaagct tcagccctat cattcggcgc gccgcggccg cagcataggg
     1861 atccccgaat tccgaagttc ctattctcta gaaagtatag gaacttcagg tctgaagagg
     1921 agtttacgtc cagccaagct agcttggctg caggtcgtcg aaattctacc gggtagggga
     1981 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg
     2041 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc
     2101 tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc
     2161 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag
     2221 tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg
     2281 ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg
     2341 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc
     2401 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc
     2461 gggcctttcg acctgcagcc tgttgacaat taatcatcgg catagtatat cggcatagta
     2521 taatacgaca aggtgaggaa ctaaaccatg ggatcggcca ttgaacaaga tggattgcac
     2581 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     2641 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     2701 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
     2761 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     2821 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     2881 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     2941 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     3001 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     3061 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg atgatctcgt cgtgacccat
     3121 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     3181 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     3241 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     3301 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg aggggatcaa
     3361 ttctctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     3421 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     3481 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     3541 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     3601 gtgggctcta tggcttctga ggcggaaaga accagctggg gctcgactag agcttgcgga
     3661 acccttcgtt gaagttccta ttctctagaa agtataggaa cttcagtcga cgtgctccaa
     3721 ggagagtttg ctggcaagat cttcggggtc gatgctaaca cagggaatgt gcttgcctat
     3781 gagaggctag accgggagaa agtctctgag tacttcctta ctgccctcat tgtggacaag
     3841 aacaccaaca aaaacctgga acaaccttcc agcttcactg tcaaggtgca tgatataaat
     3901 gacaattggc ctgtgttttc gcaccaggta ttcaacgcat ctgtgccaga gatgtcagct
     3961 ataggtatgc acctagtctt ccctctctcc tcccttcctc aacaccagcc tgaagacacc
     4021 tctcacatca gccacctcaa tgcttagggt caggctccag taagctcaag gaagtagttg
     4081 taatgctatc ttgtgccttt acatgatttc aaaatgtgtc cctaaaggct aaactctgac
     4141 ctagccccaa aataaaccct gaccctaatc atagatgggt tttaatcttc acaatgtgct
     4201 gaacactgac tctcattata gcctgggcct tgatcctgac cgcagagtgt gctgtcaccc
     4261 tagatatggg cttaggaggt actagcctgt cttcatcctg cttacagaag gtccaggcaa
     4321 agagtgtgtc tctctctact cttctgtctg ggatctgtgc tcaaggccat ggagccagac
     4381 cttcttgagg aaacccagaa ctccaaagga tgagaaatgg aatctcaacc cctctgggaa
     4441 ctttgcaaca cccttcctag tcccgcattc atactttcag aaaatgaaaa tctacccaga
     4501 cgtcacctat gtttctctct caaacccaac cccttccctt cttcatttct cccacaaaca
     4561 cacacaccac ctgttctctc tgcctgcttc atgactgctc cggctggcac tttgtcctca
     4621 tgatggcccc tctagcacaa ggcagccttc attccctcat cttgggcagt aggaacagca
     4681 gaacccccat ctactcccac caggaaatgg ggtgagggcc agtcatgagc ggacatacca
     4741 gaattctctc tctccacagg gacctctgtc atccgtgtga cagcagtgga tgcagatgac
     4801 cccactgtgg ccggccacgc cactgtcttg taccaaatcg tgaaaggaaa tgagtatttc
     4861 agcattgata attctggtct gtatgactaa gccttttggg cgggtaccca tttggaggga
     4921 tggaggaggg acgtgaatat tgcaggtacc agtgtataga gaacattaac ctgataggtg
     4981 tcaccagcca ctgatccaga gaaggaatgc ataatacaca taaagacatg ttggagtggg
     5041 ttaggggaga aggaagcaga caagcgccca aagcaggacc tcaagtccag aaagaagatc
     5101 ggggcctttt cacccaagca tagaccagga ctcaacagaa catgctggtt ggaaagtctt
     5161 ttgtacacat tcagagacat ctgatttgaa cccagaacta ctgctcagcc cttaattaag
     5221 aattcgtaat catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     5281 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     5341 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     5401 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     5461 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     5521 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     5581 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     5641 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     5701 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     5761 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     5821 cgctttctca aagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     5881 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     5941 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     6001 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6061 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     6121 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     6181 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     6241 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     6301 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     6361 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     6421 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     6481 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     6541 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     6601 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     6661 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     6721 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     6781 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     6841 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     6901 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     6961 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     7021 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     7081 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     7141 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     7201 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     7261 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     7321 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     7381 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     7441 cgaggccctt tcgtc
//
