LOCUS Exported 7798 bp ds-DNA circular SYN 10-AUG-2017 DEFINITION pcDNA3.3 TOPO TA. ACCESSION urn.local...3pz935c-p VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7798) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 23, 2017 from SnapGene 4.0.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7798 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 51..627 misc_feature 130..417 misc_feature 584..604 promoter 585..654 promoter 597..716 misc_feature 628..647 misc_feature 742..747 misc_feature 748..4863 misc_feature 4864..4884 misc_feature 4885..4896 misc_feature complement(4890..4896) /label=Geneious type: primer_bind_reverse misc_feature 4897..5400 primer_bind 5401..5435 misc_feature 5404..5405 /label=Geneious type: Editing History Replacement primer_bind 5406..5435 terminator 5439..5708 rep_origin complement(5843..6052) /direction=LEFT rep_origin complement(6055..6462) /direction=LEFT gene complement(6617..7477) /gene="Ampicillin" /label=Ampicillin promoter complement(7519..7547) ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg 661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg 721 actctagagg atcgaaccct tgccaccatg gacaagaagt actccattgg gctcgctatc 781 ggcacaaaca gcgtcggctg ggccgtcatt acggacgagt acaaggtgcc gagcaaaaaa 841 ttcaaagttc tgggcaatac cgatcgccac agcataaaga agaacctcat tggcgccctc 901 ctgttcgact ccggggagac ggccgaagcc acgcggctca aaagaacagc acggcgcaga 961 tatacccgca gaaagaatcg gatctgctac ctgcaggaga tctttagtaa tgagatggct 1021 aaggtggatg actctttctt ccataggctg gaggagtcct ttttggtgga ggaggataaa 1081 aagcacgagc gccacccaat ctttggcaat atcgtggacg aggtggcgta ccatgaaaag 1141 tacccaacca tatatcatct gaggaagaag cttgtagaca gtactgataa ggctgacttg 1201 cggttgatct atctcgcgct ggcgcatatg atcaaatttc ggggacactt cctcatcgag 1261 ggggacctga acccagacaa cagcgatgtc gataaactct ttatccaact ggttcagact 1321 tacaatcagc ttttcgaaga gaacccgatc aacgcatccg gagttgacgc caaagcaatc 1381 ctgagcgcta ggctgtccaa atcccggcgg ctcgaaaacc tcatcgcaca gctccctggg 1441 gagaagaaga acggcctgtt tggtaatctt atcgccctgt cactcgggct gacccccaac 1501 tttaaatcta acttcgacct ggccgaagat gccaagcttc aactgagcaa agacacctac 1561 gatgatgatc tcgacaatct gctggcccag atcggcgacc agtacgcaga cctttttttg 1621 gcggcaaaga acctgtcaga cgccattctg ctgagtgata ttctgcgagt gaacacggag 1681 atcaccaaag ctccgctgag cgctagtatg atcaagcgct atgatgagca ccaccaagac 1741 ttgactttgc tgaaggccct tgtcagacag caactgcctg agaagtacaa ggaaattttc 1801 ttcgatcagt ctaaaaatgg ctacgccgga tacattgacg gcggagcaag ccaggaggaa 1861 ttttacaaat ttattaagcc catcttggaa aaaatggacg gcaccgagga gctgctggta 1921 aagcttaaca gagaagatct gttgcgcaaa cagcgcactt tcgacaatgg aagcatcccc 1981 caccagattc acctgggcga actgcacgct atcctcaggc ggcaagagga tttctacccc 2041 tttttgaaag ataacaggga aaagattgag aaaatcctca catttcggat accctactat 2101 gtaggccccc tcgcccgggg aaattccaga ttcgcgtgga tgactcgcaa atcagaagag 2161 accatcactc cctggaactt cgaggaagtc gtggataagg gggcctctgc ccagtccttc 2221 atcgaaagga tgactaactt tgataaaaat ctgcctaacg aaaaggtgct tcctaaacac 2281 tctctgctgt acgagtactt cacagtttat aacgagctca ccaaggtcaa atacgtcaca 2341 gaagggatga gaaagccagc attcctgtct ggagagcaga agaaagctat cgtggacctc 2401 ctcttcaaga cgaaccggaa agttaccgtg aaacagctca aagaagacta tttcaaaaag 2461 attgaatgtt tcgactctgt tgaaatcagc ggagtggagg atcgcttcaa cgcatccctg 2521 ggaacgtatc acgatctcct gaaaatcatt aaagacaagg acttcctgga caatgaggag 2581 aacgaggaca ttcttgagga cattgtcctc acccttacgt tgtttgaaga tagggagatg 2641 attgaagaac gcttgaaaac ttacgctcat ctcttcgacg acaaagtcat gaaacagctc 2701 aagaggcgcc gatatacagg atgggggcgg ctgtcaagaa aactgatcaa tgggatccga 2761 gacaagcaga gtggaaagac aatcctggat tttcttaagt ccgatggatt tgccaaccgg 2821 aacttcatgc agttgatcca tgatgactct ctcaccttta aggaggacat ccagaaagca 2881 caagtttctg gccaggggga cagtcttcac gagcacatcg ctaatcttgc aggtagccca 2941 gctatcaaaa agggaatact gcagaccgtt aaggtcgtgg atgaactcgt caaagtaatg 3001 ggaaggcata agcccgagaa tatcgttatc gagatggccc gagagaacca aactacccag 3061 aagggacaga agaacagtag ggaaaggatg aagaggattg aagagggtat aaaagaactg 3121 gggtcccaaa tccttaagga acacccagtt gaaaacaccc agcttcagaa tgagaagctc 3181 tacctgtact acctgcagaa cggcagggac atgtacgtgg atcaggaact ggacatcaat 3241 cggctctccg actacgacgt ggctgctatc gtgccccagt cttttctcaa agatgattct 3301 attgataata aagtgttgac aagatccgat aaagctagag ggaagagtga taacgtcccc 3361 tcagaagaag ttgtcaagaa aatgaaaaat tattggcggc agctgctgaa cgccaaactg 3421 atcacacaac ggaagttcga taatctgact aaggctgaac gaggtggcct gtctgagttg 3481 gataaagccg gcttcatcaa aaggcagctt gttgagacac gccagatcac caagcacgtg 3541 gcccaaattc tcgattcacg catgaacacc aagtacgatg aaaatgacaa actgattcga 3601 gaggtgaaag ttattactct gaagtctaag ctggtctcag atttcagaaa ggactttcag 3661 ttttataagg tgagagagat caacaattac caccatgcgc atgatgccta cctgaatgca 3721 gtggtaggca ctgcacttat caaaaaatat cccaagcttg aatctgaatt tgtttacgga 3781 gactataaag tgtacgatgt taggaaaatg atcgcaaagt ctgagcagga aataggcaag 3841 gccaccgcta agtacttctt ttacagcaat attatgaatt ttttcaagac cgagattaca 3901 ctggccaatg gagagattcg gaagcgacca cttatcgaaa caaacggaga aacaggagaa 3961 atcgtgtggg acaagggtag ggatttcgcg acagtccgga aggtcctgtc catgccgcag 4021 gtgaacatcg ttaaaaagac cgaagtacag accggaggct tctccaagga aagtatcctc 4081 ccgaaaagga acagcgacaa gctgatcgca cgcaaaaaag attgggaccc caagaaatac 4141 ggcggattcg attctcctac agtcgcttac agtgtactgg ttgtggccaa agtggagaaa 4201 gggaagtcta aaaaactcaa aagcgtcaag gaactgctgg gcatcacaat catggagcga 4261 tcaagcttcg aaaaaaaccc catcgacttt ctcgaggcga aaggatataa agaggtcaaa 4321 aaagacctca tcattaagct tcccaagtac tctctctttg agcttgaaaa cggccggaaa 4381 cgaatgctcg ctagtgcggg cgagctgcag aaaggtaacg agctggcact gccctctaaa 4441 tacgttaatt tcttgtatct ggccagccac tatgaaaagc tcaaagggtc tcccgaagat 4501 aatgagcaga agcagctgtt cgtggaacaa cacaaacact accttgatga gatcatcgag 4561 caaataagcg aattctccaa aagagtgatc ctcgccgacg ctaacctcga taaggtgctt 4621 tctgcttaca ataagcacag ggataagccc atcagggagc aggcagaaaa cattatccac 4681 ttgtttactc tgaccaactt gggcgcgcct gcagccttca agtacttcga caccaccata 4741 gacagaaagc ggtacacctc tacaaaggag gtcctggacg ccacactgat tcatcagtca 4801 attacggggc tctatgaaac aagaatcgac ctctctcagc tcggtggaga cagcagggct 4861 gaccccaaga agaagaggaa ggtgagtggt ggaggaagtg gcgggtcagg gtcggtgctg 4921 cctcaggctc ctgctcctgc accagctcca gccatggtgt ctgcactggc tcaggcacca 4981 gcacccgtgc ctgtgctggc tcctggacct ccacaggctg tggctccacc agcccctaaa 5041 cctacacagg ccggcgaggg cacactgtct gaagctctgc tgcagctgca gttcgacgac 5101 gaggatctgg gagccctgct gggaaacagc accgatcctg ccgtgttcac cgacctggcc 5161 agcgtggaca acagcgagtt ccagcagctg ctgaaccagg gcatccctgt ggcccctcac 5221 accaccgagc ccatgctgat ggaatacccc gaggccatca cccggctcgt gacaggcgct 5281 cagaggcctc ctgatccagc tcctgcccct ctgggagcac caggcctgcc taatggactg 5341 ctgtctggcg acgaggactt cagctctatc gccgatatgg atttctcagc cttgctgtag 5401 aagaattcga tccctaccgg ttagtaatga gtttaaacgg gggaggctaa ctgaaacacg 5461 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac 5521 gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg 5581 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac 5641 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc 5701 ctccatagtc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 5761 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 5821 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 5881 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 5941 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 6001 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 6061 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 6121 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 6181 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 6241 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga 6301 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 6361 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 6421 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 6481 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 6541 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 6601 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 6661 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 6721 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 6781 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 6841 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 6901 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 6961 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 7021 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 7081 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 7141 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 7201 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 7261 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 7321 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 7381 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 7441 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 7501 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 7561 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat 7621 cgggagatct cccgatcccc tatggtcgac tctcagtaca atctgctctg atgccgcata 7681 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa 7741 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagg //