LOCUS Exported 8278 bp ds-DNA circular SYN 10-AUG-2017 DEFINITION pcDNA3.3 TOPO TA. ACCESSION urn.local...3pz935c-p VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8278) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 24, 2017 from SnapGene 4.0.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8278 /organism="synthetic DNA construct" /mol_type="other DNA" source 4915..5136 source 5167..5649 promoter 51..627 misc_feature 130..417 misc_feature 584..604 promoter 585..654 promoter 597..716 misc_feature 628..647 misc_feature 742..747 misc_feature 748..4863 misc_feature 4864..4884 misc_feature 4885..4895 misc_feature 4900..5146 /label=Geneious type: Editing History Replacement misc_feature 4915..5136 /label=Geneious type: c9_ad_domain misc_feature 5166..5167 /label=Geneious type: Editing History Deletion misc_feature 5167..5649 /label=Geneious type: c9_ad_domain misc_feature 5679..5680 /label=Geneious type: Editing History Deletion misc_feature 5680..5877 /label=Geneious type: ORF primer_bind 5878..5915 misc_feature 5878..5880 misc_feature complement(5878..5880) primer_bind 5886..5915 terminator 5919..6188 rep_origin complement(6323..6532) /direction=LEFT rep_origin complement(6535..6942) /direction=LEFT gene complement(7097..7957) /gene="Ampicillin" /label=Ampicillin promoter complement(7999..8027) ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg 661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg 721 actctagagg atcgaaccct tgccaccatg gacaagaagt actccattgg gctcgctatc 781 ggcacaaaca gcgtcggctg ggccgtcatt acggacgagt acaaggtgcc gagcaaaaaa 841 ttcaaagttc tgggcaatac cgatcgccac agcataaaga agaacctcat tggcgccctc 901 ctgttcgact ccggggagac ggccgaagcc acgcggctca aaagaacagc acggcgcaga 961 tatacccgca gaaagaatcg gatctgctac ctgcaggaga tctttagtaa tgagatggct 1021 aaggtggatg actctttctt ccataggctg gaggagtcct ttttggtgga ggaggataaa 1081 aagcacgagc gccacccaat ctttggcaat atcgtggacg aggtggcgta ccatgaaaag 1141 tacccaacca tatatcatct gaggaagaag cttgtagaca gtactgataa ggctgacttg 1201 cggttgatct atctcgcgct ggcgcatatg atcaaatttc ggggacactt cctcatcgag 1261 ggggacctga acccagacaa cagcgatgtc gataaactct ttatccaact ggttcagact 1321 tacaatcagc ttttcgaaga gaacccgatc aacgcatccg gagttgacgc caaagcaatc 1381 ctgagcgcta ggctgtccaa atcccggcgg ctcgaaaacc tcatcgcaca gctccctggg 1441 gagaagaaga acggcctgtt tggtaatctt atcgccctgt cactcgggct gacccccaac 1501 tttaaatcta acttcgacct ggccgaagat gccaagcttc aactgagcaa agacacctac 1561 gatgatgatc tcgacaatct gctggcccag atcggcgacc agtacgcaga cctttttttg 1621 gcggcaaaga acctgtcaga cgccattctg ctgagtgata ttctgcgagt gaacacggag 1681 atcaccaaag ctccgctgag cgctagtatg atcaagcgct atgatgagca ccaccaagac 1741 ttgactttgc tgaaggccct tgtcagacag caactgcctg agaagtacaa ggaaattttc 1801 ttcgatcagt ctaaaaatgg ctacgccgga tacattgacg gcggagcaag ccaggaggaa 1861 ttttacaaat ttattaagcc catcttggaa aaaatggacg gcaccgagga gctgctggta 1921 aagcttaaca gagaagatct gttgcgcaaa cagcgcactt tcgacaatgg aagcatcccc 1981 caccagattc acctgggcga actgcacgct atcctcaggc ggcaagagga tttctacccc 2041 tttttgaaag ataacaggga aaagattgag aaaatcctca catttcggat accctactat 2101 gtaggccccc tcgcccgggg aaattccaga ttcgcgtgga tgactcgcaa atcagaagag 2161 accatcactc cctggaactt cgaggaagtc gtggataagg gggcctctgc ccagtccttc 2221 atcgaaagga tgactaactt tgataaaaat ctgcctaacg aaaaggtgct tcctaaacac 2281 tctctgctgt acgagtactt cacagtttat aacgagctca ccaaggtcaa atacgtcaca 2341 gaagggatga gaaagccagc attcctgtct ggagagcaga agaaagctat cgtggacctc 2401 ctcttcaaga cgaaccggaa agttaccgtg aaacagctca aagaagacta tttcaaaaag 2461 attgaatgtt tcgactctgt tgaaatcagc ggagtggagg atcgcttcaa cgcatccctg 2521 ggaacgtatc acgatctcct gaaaatcatt aaagacaagg acttcctgga caatgaggag 2581 aacgaggaca ttcttgagga cattgtcctc acccttacgt tgtttgaaga tagggagatg 2641 attgaagaac gcttgaaaac ttacgctcat ctcttcgacg acaaagtcat gaaacagctc 2701 aagaggcgcc gatatacagg atgggggcgg ctgtcaagaa aactgatcaa tgggatccga 2761 gacaagcaga gtggaaagac aatcctggat tttcttaagt ccgatggatt tgccaaccgg 2821 aacttcatgc agttgatcca tgatgactct ctcaccttta aggaggacat ccagaaagca 2881 caagtttctg gccaggggga cagtcttcac gagcacatcg ctaatcttgc aggtagccca 2941 gctatcaaaa agggaatact gcagaccgtt aaggtcgtgg atgaactcgt caaagtaatg 3001 ggaaggcata agcccgagaa tatcgttatc gagatggccc gagagaacca aactacccag 3061 aagggacaga agaacagtag ggaaaggatg aagaggattg aagagggtat aaaagaactg 3121 gggtcccaaa tccttaagga acacccagtt gaaaacaccc agcttcagaa tgagaagctc 3181 tacctgtact acctgcagaa cggcagggac atgtacgtgg atcaggaact ggacatcaat 3241 cggctctccg actacgacgt ggctgctatc gtgccccagt cttttctcaa agatgattct 3301 attgataata aagtgttgac aagatccgat aaagctagag ggaagagtga taacgtcccc 3361 tcagaagaag ttgtcaagaa aatgaaaaat tattggcggc agctgctgaa cgccaaactg 3421 atcacacaac ggaagttcga taatctgact aaggctgaac gaggtggcct gtctgagttg 3481 gataaagccg gcttcatcaa aaggcagctt gttgagacac gccagatcac caagcacgtg 3541 gcccaaattc tcgattcacg catgaacacc aagtacgatg aaaatgacaa actgattcga 3601 gaggtgaaag ttattactct gaagtctaag ctggtctcag atttcagaaa ggactttcag 3661 ttttataagg tgagagagat caacaattac caccatgcgc atgatgccta cctgaatgca 3721 gtggtaggca ctgcacttat caaaaaatat cccaagcttg aatctgaatt tgtttacgga 3781 gactataaag tgtacgatgt taggaaaatg atcgcaaagt ctgagcagga aataggcaag 3841 gccaccgcta agtacttctt ttacagcaat attatgaatt ttttcaagac cgagattaca 3901 ctggccaatg gagagattcg gaagcgacca cttatcgaaa caaacggaga aacaggagaa 3961 atcgtgtggg acaagggtag ggatttcgcg acagtccgga aggtcctgtc catgccgcag 4021 gtgaacatcg ttaaaaagac cgaagtacag accggaggct tctccaagga aagtatcctc 4081 ccgaaaagga acagcgacaa gctgatcgca cgcaaaaaag attgggaccc caagaaatac 4141 ggcggattcg attctcctac agtcgcttac agtgtactgg ttgtggccaa agtggagaaa 4201 gggaagtcta aaaaactcaa aagcgtcaag gaactgctgg gcatcacaat catggagcga 4261 tcaagcttcg aaaaaaaccc catcgacttt ctcgaggcga aaggatataa agaggtcaaa 4321 aaagacctca tcattaagct tcccaagtac tctctctttg agcttgaaaa cggccggaaa 4381 cgaatgctcg ctagtgcggg cgagctgcag aaaggtaacg agctggcact gccctctaaa 4441 tacgttaatt tcttgtatct ggccagccac tatgaaaagc tcaaagggtc tcccgaagat 4501 aatgagcaga agcagctgtt cgtggaacaa cacaaacact accttgatga gatcatcgag 4561 caaataagcg aattctccaa aagagtgatc ctcgccgacg ctaacctcga taaggtgctt 4621 tctgcttaca ataagcacag ggataagccc atcagggagc aggcagaaaa cattatccac 4681 ttgtttactc tgaccaactt gggcgcgcct gcagccttca agtacttcga caccaccata 4741 gacagaaagc ggtacacctc tacaaaggag gtcctggacg ccacactgat tcatcagtca 4801 attacggggc tctatgaaac aagaatcgac ctctctcagc tcggtggaga cagcagggct 4861 gaccccaaga agaagaggaa ggtgagtggt ggaggaagtg gcgggtcagg gtcggaggcc 4921 agcggttccg gacgggctga cgcattggac gattttgatc tggatatgct gggaagtgac 4981 gccctcgatg attttgacct tgacatgctt ggttcggatg cccttgatga ctttgacctc 5041 gacatgctcg gcagtgacgc ccttgatgat ttcgacctgg acatgctgat taactctaga 5101 agttccggat ctccgaaaaa gaaacgcaaa gttggttcgg gaggtggttc gggtggctct 5161 ggatcagtgc tgcctcaggc tcctgctcct gcaccagctc cagccatggt gtctgcactg 5221 gctcaggcac cagcacccgt gcctgtgctg gctcctggac ctccacaggc tgtggctcca 5281 ccagccccta aacctacaca ggccggcgag ggcacactgt ctgaagctct gctgcagctg 5341 cagttcgacg acgaggatct gggagccctg ctgggaaaca gcaccgatcc tgccgtgttc 5401 accgacctgg ccagcgtgga caacagcgag ttccagcagc tgctgaacca gggcatccct 5461 gtggcccctc acaccaccga gcccatgctg atggaatacc ccgaggccat cacccggctc 5521 gtgacaggcg ctcagaggcc tcctgatcca gctcctgccc ctctgggagc accaggcctg 5581 cctaatggac tgctgtctgg cgacgaggac ttcagctcta tcgccgatat ggatttctca 5641 gccttgctgt caggcggtgg tagtggtggg agcggtagtg acctttccca tccgccccca 5701 aggggccatc tggatgagct gacaaccaca cttgagtcca tgaccgagga tctgaacctg 5761 gactcacccc tgaccccgga attgaacgag attctggata ccttcctgaa cgacgagtgc 5821 ctcttgcatg ccatgcatat cagcacagga ctgtccatct tcgacacatc tctgttttag 5881 aagaattcga tccctaccgg ttagtaatga gtttaaacgg gggaggctaa ctgaaacacg 5941 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac 6001 gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg 6061 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac 6121 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc 6181 ctccatagtc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 6241 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 6301 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 6361 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 6421 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 6481 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 6541 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 6601 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 6661 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 6721 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga 6781 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 6841 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 6901 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 6961 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 7021 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 7081 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 7141 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 7201 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 7261 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 7321 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 7381 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 7441 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 7501 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 7561 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 7621 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 7681 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 7741 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 7801 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 7861 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 7921 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 7981 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 8041 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat 8101 cgggagatct cccgatcccc tatggtcgac tctcagtaca atctgctctg atgccgcata 8161 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa 8221 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagg //