LOCUS Exported 8128 bp ds-DNA circular SYN 10-AUG-2017 DEFINITION pcDNA3.3 TOPO TA. ACCESSION urn.local...3pz935c-p VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8128) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 24, 2017 from SnapGene 4.0.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8128 /organism="synthetic DNA construct" /mol_type="other DNA" source 4915..5136 source 5167..5499 promoter 51..627 misc_feature 130..417 misc_feature 584..604 promoter 585..654 promoter 597..716 misc_feature 628..647 misc_feature 742..747 misc_feature 748..4863 misc_feature 4864..4884 misc_feature 4885..4895 misc_feature 4900..5146 /label=Geneious type: Editing History Replacement misc_feature 4915..5136 /label=Geneious type: c9_ad_domain misc_feature 5166..5167 /label=Geneious type: Editing History Deletion misc_feature 5167..5499 /label=Geneious type: c9_ad_domain misc_feature 5529..5530 /label=Geneious type: Editing History Deletion misc_feature 5530..5727 /label=Geneious type: ORF primer_bind 5728..5765 misc_feature 5728..5730 misc_feature complement(5728..5730) primer_bind 5736..5765 terminator 5769..6038 rep_origin complement(6173..6382) /direction=LEFT rep_origin complement(6385..6792) /direction=LEFT gene complement(6947..7807) /gene="Ampicillin" /label=Ampicillin promoter complement(7849..7877) ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg 661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg 721 actctagagg atcgaaccct tgccaccatg gacaagaagt actccattgg gctcgctatc 781 ggcacaaaca gcgtcggctg ggccgtcatt acggacgagt acaaggtgcc gagcaaaaaa 841 ttcaaagttc tgggcaatac cgatcgccac agcataaaga agaacctcat tggcgccctc 901 ctgttcgact ccggggagac ggccgaagcc acgcggctca aaagaacagc acggcgcaga 961 tatacccgca gaaagaatcg gatctgctac ctgcaggaga tctttagtaa tgagatggct 1021 aaggtggatg actctttctt ccataggctg gaggagtcct ttttggtgga ggaggataaa 1081 aagcacgagc gccacccaat ctttggcaat atcgtggacg aggtggcgta ccatgaaaag 1141 tacccaacca tatatcatct gaggaagaag cttgtagaca gtactgataa ggctgacttg 1201 cggttgatct atctcgcgct ggcgcatatg atcaaatttc ggggacactt cctcatcgag 1261 ggggacctga acccagacaa cagcgatgtc gataaactct ttatccaact ggttcagact 1321 tacaatcagc ttttcgaaga gaacccgatc aacgcatccg gagttgacgc caaagcaatc 1381 ctgagcgcta ggctgtccaa atcccggcgg ctcgaaaacc tcatcgcaca gctccctggg 1441 gagaagaaga acggcctgtt tggtaatctt atcgccctgt cactcgggct gacccccaac 1501 tttaaatcta acttcgacct ggccgaagat gccaagcttc aactgagcaa agacacctac 1561 gatgatgatc tcgacaatct gctggcccag atcggcgacc agtacgcaga cctttttttg 1621 gcggcaaaga acctgtcaga cgccattctg ctgagtgata ttctgcgagt gaacacggag 1681 atcaccaaag ctccgctgag cgctagtatg atcaagcgct atgatgagca ccaccaagac 1741 ttgactttgc tgaaggccct tgtcagacag caactgcctg agaagtacaa ggaaattttc 1801 ttcgatcagt ctaaaaatgg ctacgccgga tacattgacg gcggagcaag ccaggaggaa 1861 ttttacaaat ttattaagcc catcttggaa aaaatggacg gcaccgagga gctgctggta 1921 aagcttaaca gagaagatct gttgcgcaaa cagcgcactt tcgacaatgg aagcatcccc 1981 caccagattc acctgggcga actgcacgct atcctcaggc ggcaagagga tttctacccc 2041 tttttgaaag ataacaggga aaagattgag aaaatcctca catttcggat accctactat 2101 gtaggccccc tcgcccgggg aaattccaga ttcgcgtgga tgactcgcaa atcagaagag 2161 accatcactc cctggaactt cgaggaagtc gtggataagg gggcctctgc ccagtccttc 2221 atcgaaagga tgactaactt tgataaaaat ctgcctaacg aaaaggtgct tcctaaacac 2281 tctctgctgt acgagtactt cacagtttat aacgagctca ccaaggtcaa atacgtcaca 2341 gaagggatga gaaagccagc attcctgtct ggagagcaga agaaagctat cgtggacctc 2401 ctcttcaaga cgaaccggaa agttaccgtg aaacagctca aagaagacta tttcaaaaag 2461 attgaatgtt tcgactctgt tgaaatcagc ggagtggagg atcgcttcaa cgcatccctg 2521 ggaacgtatc acgatctcct gaaaatcatt aaagacaagg acttcctgga caatgaggag 2581 aacgaggaca ttcttgagga cattgtcctc acccttacgt tgtttgaaga tagggagatg 2641 attgaagaac gcttgaaaac ttacgctcat ctcttcgacg acaaagtcat gaaacagctc 2701 aagaggcgcc gatatacagg atgggggcgg ctgtcaagaa aactgatcaa tgggatccga 2761 gacaagcaga gtggaaagac aatcctggat tttcttaagt ccgatggatt tgccaaccgg 2821 aacttcatgc agttgatcca tgatgactct ctcaccttta aggaggacat ccagaaagca 2881 caagtttctg gccaggggga cagtcttcac gagcacatcg ctaatcttgc aggtagccca 2941 gctatcaaaa agggaatact gcagaccgtt aaggtcgtgg atgaactcgt caaagtaatg 3001 ggaaggcata agcccgagaa tatcgttatc gagatggccc gagagaacca aactacccag 3061 aagggacaga agaacagtag ggaaaggatg aagaggattg aagagggtat aaaagaactg 3121 gggtcccaaa tccttaagga acacccagtt gaaaacaccc agcttcagaa tgagaagctc 3181 tacctgtact acctgcagaa cggcagggac atgtacgtgg atcaggaact ggacatcaat 3241 cggctctccg actacgacgt ggctgctatc gtgccccagt cttttctcaa agatgattct 3301 attgataata aagtgttgac aagatccgat aaagctagag ggaagagtga taacgtcccc 3361 tcagaagaag ttgtcaagaa aatgaaaaat tattggcggc agctgctgaa cgccaaactg 3421 atcacacaac ggaagttcga taatctgact aaggctgaac gaggtggcct gtctgagttg 3481 gataaagccg gcttcatcaa aaggcagctt gttgagacac gccagatcac caagcacgtg 3541 gcccaaattc tcgattcacg catgaacacc aagtacgatg aaaatgacaa actgattcga 3601 gaggtgaaag ttattactct gaagtctaag ctggtctcag atttcagaaa ggactttcag 3661 ttttataagg tgagagagat caacaattac caccatgcgc atgatgccta cctgaatgca 3721 gtggtaggca ctgcacttat caaaaaatat cccaagcttg aatctgaatt tgtttacgga 3781 gactataaag tgtacgatgt taggaaaatg atcgcaaagt ctgagcagga aataggcaag 3841 gccaccgcta agtacttctt ttacagcaat attatgaatt ttttcaagac cgagattaca 3901 ctggccaatg gagagattcg gaagcgacca cttatcgaaa caaacggaga aacaggagaa 3961 atcgtgtggg acaagggtag ggatttcgcg acagtccgga aggtcctgtc catgccgcag 4021 gtgaacatcg ttaaaaagac cgaagtacag accggaggct tctccaagga aagtatcctc 4081 ccgaaaagga acagcgacaa gctgatcgca cgcaaaaaag attgggaccc caagaaatac 4141 ggcggattcg attctcctac agtcgcttac agtgtactgg ttgtggccaa agtggagaaa 4201 gggaagtcta aaaaactcaa aagcgtcaag gaactgctgg gcatcacaat catggagcga 4261 tcaagcttcg aaaaaaaccc catcgacttt ctcgaggcga aaggatataa agaggtcaaa 4321 aaagacctca tcattaagct tcccaagtac tctctctttg agcttgaaaa cggccggaaa 4381 cgaatgctcg ctagtgcggg cgagctgcag aaaggtaacg agctggcact gccctctaaa 4441 tacgttaatt tcttgtatct ggccagccac tatgaaaagc tcaaagggtc tcccgaagat 4501 aatgagcaga agcagctgtt cgtggaacaa cacaaacact accttgatga gatcatcgag 4561 caaataagcg aattctccaa aagagtgatc ctcgccgacg ctaacctcga taaggtgctt 4621 tctgcttaca ataagcacag ggataagccc atcagggagc aggcagaaaa cattatccac 4681 ttgtttactc tgaccaactt gggcgcgcct gcagccttca agtacttcga caccaccata 4741 gacagaaagc ggtacacctc tacaaaggag gtcctggacg ccacactgat tcatcagtca 4801 attacggggc tctatgaaac aagaatcgac ctctctcagc tcggtggaga cagcagggct 4861 gaccccaaga agaagaggaa ggtgagtggt ggaggaagtg gcgggtcagg gtcggaggcc 4921 agcggttccg gacgggctga cgcattggac gattttgatc tggatatgct gggaagtgac 4981 gccctcgatg attttgacct tgacatgctt ggttcggatg cccttgatga ctttgacctc 5041 gacatgctcg gcagtgacgc ccttgatgat ttcgacctgg acatgctgat taactctaga 5101 agttccggat ctccgaaaaa gaaacgcaaa gttggttcgg gaggtggttc gggtggctct 5161 ggatcactgt ctgaagctct gctgcagctg cagttcgacg acgaggatct gggagccctg 5221 ctgggaaaca gcaccgatcc tgccgtgttc accgacctgg ccagcgtgga caacagcgag 5281 ttccagcagc tgctgaacca gggcatccct gtggcccctc acaccaccga gcccatgctg 5341 atggaatacc ccgaggccat cacccggctc gtgacaggcg ctcagaggcc tcctgatcca 5401 gctcctgccc ctctgggagc accaggcctg cctaatggac tgctgtctgg cgacgaggac 5461 ttcagctcta tcgccgatat ggatttctca gccttgctgt caggcggtgg tagtggtggg 5521 agcggtagtg acctttccca tccgccccca aggggccatc tggatgagct gacaaccaca 5581 cttgagtcca tgaccgagga tctgaacctg gactcacccc tgaccccgga attgaacgag 5641 attctggata ccttcctgaa cgacgagtgc ctcttgcatg ccatgcatat cagcacagga 5701 ctgtccatct tcgacacatc tctgttttag aagaattcga tccctaccgg ttagtaatga 5761 gtttaaacgg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc 5821 tatgacggca ataaaaagac agaataaaac gcacgggtgt tgggtcgttt gttcataaac 5881 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc 5941 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag 6001 ggctcgcagc caacgtcggg gcggcaggcc ctccatagtc ggtcgttcgg ctgcggcgag 6061 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 6121 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 6181 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 6241 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 6301 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 6361 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 6421 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 6481 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 6541 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 6601 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 6661 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 6721 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 6781 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 6841 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 6901 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 6961 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 7021 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 7081 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 7141 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 7201 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 7261 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 7321 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 7381 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 7441 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 7501 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 7561 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 7621 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 7681 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 7741 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 7801 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 7861 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 7921 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtcgac 7981 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt 8041 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac 8101 cgacaattgc atgaagaatc tgcttagg //