LOCUS Exported 12349 bp ds-DNA circular SYN 13-JUL-2017 DEFINITION . ACCESSION . VERSION . KEYWORDS PB-UniSAM SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 12349) AUTHORS Anta TITLE Direct Submission JOURNAL Exported 13 Jul 2017 from SnapGene 2.3.2 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12349 /organism="recombinant plasmid" /mol_type="other DNA" misc_feature 3..458 /label="M13 origin" /note="M13 origin" misc_feature 13..453 /label="F1 ori" /note="F1 ori" misc_feature 463..531 /label="LacZ alpha" /note="LacZ alpha" misc_feature 602..619 /label="M13-F" /note="M13-F" misc_feature 618..645 /label="T7" /note="T7" misc_feature 707..941 /label="PB 3'LTR" /note="PB 3'LTR" promoter 1197..2374 /note="EF-1-alpha promoter" /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 1428..2365 /note="EF-1-alpha intron A" /note="intron upstream of the start codon of human EF-1-alpha" CDS 2390..2422 /codon_start=1 /locus_tag=" " /product="nuclear localization signal of SV40 large T antigen" /note="SV40 NLS" /note=" " /protein_id=" " /translation="SPKKKRKVEAS" misc_feature 2423..6523 /note="dcas9" misc_feature 6531..6574 /note="linker" CDS 6575..6607 /codon_start=1 /locus_tag=" " /product="nuclear localization signal of SV40 large T antigen" /note="SV40 NLS" /note=" " /protein_id=" " /translation="GPKKKRKVAAA" misc_feature 6614..6778 /note="VP64" misc_feature 6782..6790 /note="GSG linker" /note="Additional AA to improve the cleavage ability Hee Kim at al, 2011 Plos One" CDS 6791..6847 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein http://www.nature.com/gt/journal/v11/n23/full/3302361a.html " /note="P2A" /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" misc_feature 6848..6856 /note="GSG linker" /note="Additional AA to improve the cleavage ability Hee Kim at al, 2011 Plos One" misc_feature 6857..7246 /note="MS2" misc_feature 7253..7297 /note="Linker" CDS 7301..7321 /codon_start=1 /locus_tag=" " /product="nuclear localization signal of SV40 large T antigen" /note="SV40 NLS" /note=" " /protein_id=" " /translation="PKKKRKV" misc_feature 7337..7888 /note="p65" misc_feature 7889..7903 /note="linker" misc_feature 7904..8275 /note="HSF1" CDS 8291..8344 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /note="T2A" /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /protein_id=" " /translation="EGRGSLLTCGDVEENPGP" CDS 8345..9055 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /note="mCherry" /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" misc_feature 9062..9120 /note="synthetic polyA" polyA_signal 9072..9120 /note="synthetic polyadenylation signal" promoter 9183..9431 /label="U6 Promoter" /note="U6 promoter" /note="RNA polymerase III promoter for human U6 snRNA" misc_feature 9432..9449 /note="BsbI sites" misc_feature 9450..9585 /note="gRNA backbone" misc_feature 9462..9494 /note="M2 loop" misc_feature 9532..9564 /note="M2 loop" misc_feature 9609..9643 /note="overlap PB" misc_feature 9700..10012 /label="PB 5'LTR" /note="PB 5'LTR" misc_feature 10156..10175 /label="T3" /note="T3" misc_feature 10193..10213 /label="M13-R" /note="M13-R" misc_feature 10219..10241 /label="LacO" /note="LacO" misc_feature 10581..11263 /label="ColE1 origin" /note="ColE1 origin" misc_feature 11361..12020 /label="AmpR" /note="AmpR" ORIGIN 1 ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct 481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcgg 661 tattcacgac agcaggctga ataataaaaa aattagaaac tattatttaa ccctagaaag 721 ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta 781 tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt 841 acacttacat actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa 901 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttaaa cattctctct 961 tttacaaaaa taaacttatt ttgtacttta aaaacagtca tgttgtatta taaaataagt 1021 aattagctta acctatacat aatagaaaca aattatactt attagtcagt cagaaacaac 1081 tttggcacat atcaatatta tgctctcgtt aattaatgca aagatggata aagttttaaa 1141 cagagaggaa tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaattggct 1201 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 1261 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 1321 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 1381 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt 1441 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt 1501 ccactggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag 1561 ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg 1621 gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga 1681 taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga 1741 tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg 1801 cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg 1861 gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct 1921 cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc 1981 gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg 2041 gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc 2101 agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt 2161 ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt 2221 tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc 2281 cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt 2341 tcaaagtttt tttcttccat ttcaggtgtc gtgacgtacg gccaccatga gccccaagaa 2401 gaagagaaag gtggaggcca gcgacaagaa gtacagcatc ggcctggcca tcggcaccaa 2461 ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg cccagcaaga aattcaaggt 2521 gctgggcaac accgaccggc acagcatcaa gaagaacctg atcggagccc tgctgttcga 2581 cagcggcgaa acagccgagg ccacccggct gaagagaacc gccagaagaa gatacaccag 2641 acggaagaac cggatctgct atctgcaaga gatcttcagc aacgagatgg ccaaggtgga 2701 cgacagcttc ttccacagac tggaagagtc cttcctggtg gaagaggata agaagcacga 2761 gcggcacccc atcttcggca acatcgtgga cgaggtggcc taccacgaga agtaccccac 2821 catctaccac ctgagaaaga aactggtgga cagcaccgac aaggccgacc tgcggctgat 2881 ctatctggcc ctggcccaca tgatcaagtt ccggggccac ttcctgatcg agggcgacct 2941 gaaccccgac aacagcgacg tggacaagct gttcatccag ctggtgcaga cctacaacca 3001 gctgttcgag gaaaacccca tcaacgccag cggcgtggac gccaaggcca tcctgtctgc 3061 cagactgagc aagagcagac ggctggaaaa tctgatcgcc cagctgcccg gcgagaagaa 3121 gaatggcctg ttcggcaacc tgattgccct gagcctgggc ctgaccccca acttcaagag 3181 caacttcgac ctggccgagg atgccaaact gcagctgagc aaggacacct acgacgacga 3241 cctggacaac ctgctggccc agatcggcga ccagtacgcc gacctgtttc tggccgccaa 3301 gaacctgtcc gacgccatcc tgctgagcga catcctgaga gtgaacaccg agatcaccaa 3361 ggcccccctg agcgcctcta tgatcaagag atacgacgag caccaccagg acctgaccct 3421 gctgaaagct ctcgtgcggc agcagctgcc tgagaagtac aaagagattt tcttcgacca 3481 gagcaagaac ggctacgccg gctacattga cggcggagcc agccaggaag agttctacaa 3541 gttcatcaag cccatcctgg aaaagatgga cggcaccgag gaactgctcg tgaagctgaa 3601 cagagaggac ctgctgcgga agcagcggac cttcgacaac ggcagcatcc cccaccagat 3661 ccacctggga gagctgcacg ccattctgcg gcggcaggaa gatttttacc cattcctgaa 3721 ggacaaccgg gaaaagatcg agaagatcct gaccttccgc atcccctact acgtgggccc 3781 tctggccagg ggaaacagca gattcgcctg gatgaccaga aagagcgagg aaaccatcac 3841 cccctggaac ttcgaggaag tggtggacaa gggcgcttcc gcccagagct tcatcgagcg 3901 gatgaccaac ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc acagcctgct 3961 gtacgagtac ttcaccgtgt ataacgagct gaccaaagtg aaatacgtga ccgagggaat 4021 gagaaagccc gccttcctga gcggcgagca gaaaaaggcc atcgtggacc tgctgttcaa 4081 gaccaaccgg aaagtgaccg tgaagcagct gaaagaggac tacttcaaga aaatcgagtg 4141 cttcgactcc gtggaaatct ccggcgtgga agatcggttc aacgcctccc tgggcacata 4201 ccacgatctg ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg aaaacgagga 4261 cattctggaa gatatcgtgc tgaccctgac actgtttgag gacagagaga tgatcgagga 4321 acggctgaaa acctatgccc acctgttcga cgacaaagtg atgaagcagc tgaagcggcg 4381 gagatacacc ggctggggca ggctgagccg gaagctgatc aacggcatcc gggacaagca 4441 gtccggcaag acaatcctgg atttcctgaa gtccgacggc ttcgccaaca gaaacttcat 4501 gcagctgatc cacgacgaca gcctgacctt taaagaggac atccagaaag cccaggtgtc 4561 cggccagggc gatagcctgc acgagcacat tgccaatctg gccggcagcc ccgccattaa 4621 gaagggcatc ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga tgggccggca 4681 caagcccgag aacatcgtga tcgaaatggc cagagagaac cagaccaccc agaagggaca 4741 gaagaacagc cgcgagagaa tgaagcggat cgaagagggc atcaaagagc tgggcagcca 4801 gatcctgaaa gaacaccccg tggaaaacac ccagctgcag aacgagaagc tgtacctgta 4861 ctacctgcag aatgggcggg atatgtacgt ggaccaggaa ctggacatca accggctgtc 4921 cgactacgat gtggacgcta tcgtgcctca gagctttctg aaggacgact ccatcgacaa 4981 caaggtgctg accagaagcg acaagaaccg gggcaagagc gacaacgtgc cctccgaaga 5041 ggtcgtgaag aagatgaaga actactggcg gcagctgctg aacgccaagc tgattaccca 5101 gagaaagttc gacaatctga ccaaggccga gagaggcggc ctgagcgaac tggataaggc 5161 cggcttcatc aagagacagc tggtggaaac ccggcagatc acaaagcacg tggcacagat 5221 cctggactcc cggatgaaca ctaagtacga cgagaatgac aagctgatcc gggaagtgaa 5281 agtgatcacc ctgaagtcca agctggtgtc cgatttccgg aaggatttcc agttttacaa 5341 agtgcgcgag atcaacaact accaccacgc ccacgacgcc tacctgaacg ccgtcgtggg 5401 aaccgccctg atcaaaaagt accctaagct ggaaagcgag ttcgtgtacg gcgactacaa 5461 ggtgtacgac gtgcggaaga tgatcgccaa gagcgagcag gaaatcggca aggctaccgc 5521 caagtacttc ttctacagca acatcatgaa ctttttcaag accgagatta ccctggccaa 5581 cggcgagatc cggaagcggc ctctgatcga gacaaacggc gaaaccgggg agatcgtgtg 5641 ggataagggc cgggattttg ccaccgtgcg gaaagtgctg agcatgcccc aagtgaatat 5701 cgtgaaaaag accgaggtgc agacaggcgg cttcagcaaa gagtctatcc tgcccaagag 5761 gaacagcgat aagctgatcg ccagaaagaa ggactgggac cctaagaagt acggcggctt 5821 cgacagcccc accgtggcct attctgtgct ggtggtggcc aaagtggaaa agggcaagtc 5881 caagaaactg aagagtgtga aagagctgct ggggatcacc atcatggaaa gaagcagctt 5941 cgagaagaat cccatcgact ttctggaagc caagggctac aaagaagtga aaaaggacct 6001 gatcatcaag ctgcctaagt actccctgtt cgagctggaa aacggccgga agagaatgct 6061 ggcctctgcc ggcgaactgc agaagggaaa cgaactggcc ctgccctcca aatatgtgaa 6121 cttcctgtac ctggccagcc actatgagaa gctgaagggc tcccccgagg ataatgagca 6181 gaaacagctg tttgtggaac agcacaagca ctacctggac gagatcatcg agcagatcag 6241 cgagttctcc aagagagtga tcctggccga cgctaatctg gacaaagtgc tgtccgccta 6301 caacaagcac cgggataagc ccatcagaga gcaggccgag aatatcatcc acctgtttac 6361 cctgaccaat ctgggagccc ctgccgcctt caagtacttt gacaccacca tcgaccggaa 6421 gaggtacacc agcaccaaag aggtgctgga cgccaccctg atccaccaga gcatcaccgg 6481 cctgtacgag acacggatcg acctgtctca gctgggaggc gacagcgctg gaggaggtgg 6541 aagcggagga ggaggaagcg gaggaggagg tagcggacct aagaaaaaga ggaaggtggc 6601 ggccgctgga tccggacggg ctgacgcatt ggacgatttt gatctggata tgctgggaag 6661 tgacgccctc gatgattttg accttgacat gcttggttcg gatgcccttg atgactttga 6721 cctcgacatg ctcggcagtg acgcccttga tgatttcgac ctggacatgc tgattaacgg 6781 aggaagcgga gctactaact tcagcctgct gaagcaggct ggagacgtgg aggagaaccc 6841 tggacctgga agcggaatgg cttcaaactt tactcagttc gtgctcgtgg acaatggtgg 6901 gacaggggat gtgacagtgg ctccttctaa tttcgctaat ggggtggcag agtggatcag 6961 ctccaactca cggagccagg cctacaaggt gacatgcagc gtcaggcagt ctagtgccca 7021 gaagagaaag tataccatca aggtggaggt ccccaaagtg gctacccaga cagtgggcgg 7081 agtcgaactg cctgtcgccg cttggaggtc ctacctgaac atggagctca ctatcccaat 7141 tttcgctacc aattctgact gtgaactcat cgtgaaggca atgcaggggc tcctcaaaga 7201 cggtaatcct atcccttccg ccatcgccgc taactcaggt atctacagcg ctggaggagg 7261 tggaagcgga ggaggaggaa gcggaggagg aggtagcgga cctaagaaaa agaggaaggt 7321 ggcggccgct ggatcccctt cagggcagat cagcaaccag gccctggctc tggcccctag 7381 ctccgctcca gtgctggccc agactatggt gccctctagt gctatggtgc ctctggccca 7441 gccacctgct ccagcccctg tgctgacccc aggaccaccc cagtcactga gcgctccagt 7501 gcccaagtct acacaggccg gcgaggggac tctgagtgaa gctctgctgc acctgcagtt 7561 cgacgctgat gaggacctgg gagctctgct ggggaacagc accgatcccg gagtgttcac 7621 agatctggcc tccgtggaca actctgagtt tcagcagctg ctgaatcagg gcgtgtccat 7681 gtctcatagt acagccgaac caatgctgat ggagtacccc gaagccatta cccggctggt 7741 gaccggcagc cagcggcccc ccgaccccgc tccaactccc ctgggaacca gcggcctgcc 7801 taatgggctg tccggagatg aggacttctc aagcatcgct gatatggact ttagtgccct 7861 gctgtcacag atttcctcta gtgggcaggg aggaggtgga agcggcttca gcgtggacac 7921 cagtgccctg ctggacctgt tcagcccctc ggtgaccgtg cccgacatga gcctgcctga 7981 ccttgacagc agcctggcca gtatccaaga gctcctgtct ccccaggagc cccccaggcc 8041 tcccgaggca gagaacagca gcccggattc agggaagcag ctggtgcact acacagcgca 8101 gccgctgttc ctgctggacc ccggctccgt ggacaccggg agcaacgacc tgccggtgct 8161 gtttgagctg ggagagggct cctacttctc cgaaggggac ggcttcgccg aggaccccac 8221 catctccctg ctgacaggct cggagcctcc caaagccaag gaccccactg tctccgctag 8281 cggcagtgga gagggcagag gaagtctgct aacatgcggt gacgtcgagg agaatcctgg 8341 cccaatggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt 8401 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga 8461 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct 8521 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa 8581 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga 8641 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca 8701 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc 8761 cgtaatgcag aagaaaacca tgggctggga ggcctcctcc gagcggatgt accccgagga 8821 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga 8881 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa 8941 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta 9001 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agtagtgata 9061 aaagcggccg caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg 9121 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcagcta 9181 gcgagggcct atttcccatg attccttcat atttgcatat acgatacaag gctgttagag 9241 agataattgg aattaatttg actgtaaaca caaagatatt agtacaaaat acgtgacgta 9301 gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc 9361 atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag 9421 gacgaaacac cgggtcttcg agaagacctg ttttagagct aggccaacat gaggatcacc 9481 catgtctgca gggcctagca agttaaaata aggctagtcc gttatcaact tggccaacat 9541 gaggatcacc catgtctgca gggccaagtg gcaccgagtc ggtgcttttt ttgaattctt 9601 taattaagct agccaacaag ctcgtcatcg ctttgcagaa gagcagagag gatatgctca 9661 tcgtctaaag aactacccat tttattatat attagtcacg atatctataa caagaaaata 9721 tatatataat aagttatcac gtaagtagaa catgaaataa caatataatt atcgtatgag 9781 ttaaatctta aaagtcacgt aaaagataat catgcgtcat tttgactcac gcggtcgtta 9841 tagttcaaaa tcagtgacac ttaccgcatt gacaagcacg cctcacggga gctccaagcg 9901 gcgactgaga tgtcctaaat gcacagcgac ggattcgcgc tatttagaaa gagagagcaa 9961 tatttcaaga atgcatgcgt caattttacg cagactatct ttctagggtt aaaaaagatt 10021 tgcgctttac tcgacctaaa ctttaaacac gtcatagaat cttcgtttga caaaaaccac 10081 attgtggcca agctgtgtga cgcgacgcgc gctaaagaat ggcaaaccaa gtcgcgcgag 10141 gtacccagct tttgttccct ttagtgaggg ttaattccga gcttggcgta atcatggtca 10201 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 10261 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 10321 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 10381 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 10441 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 10501 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 10561 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 10621 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 10681 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 10741 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 10801 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 10861 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 10921 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 10981 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 11041 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 11101 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 11161 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 11221 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 11281 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 11341 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 11401 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 11461 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 11521 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 11581 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 11641 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 11701 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 11761 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 11821 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 11881 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 11941 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 12001 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 12061 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 12121 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 12181 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 12241 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 12301 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctg //