LOCUS       Exported                3003 bp ds-DNA     circular SYN 01-JUL-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pFUS_A8
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Invitrogen
  TITLE     Direct Submission
  JOURNAL   Exported Saturday, Jul 1, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
COMMENT     ORIGDB|Private
            SSBMSPEC|10836|NONE|Private|0
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="synthetic DNA construct"
                     /note="dnas_title=pFUS_A8"
                     /note="color: #ffffff"
     primer_bind     1..20
                     /label=pCR8_F1
                     /note="dnas_title=pCR8_F1
                     vntifkey=28"
                     /note="color: #0000ff; direction: RIGHT"
     misc_feature    90..143
                     /label=FUS_A_left
                     /note="dnas_title=FUS_A_left
                     vntifkey=21"
                     /note="color: #66cc99; direction: RIGHT"
     misc_feature    131
                     /label=CUT 1
                     /note="dnas_title=CUT 1
                     vntifkey=21"
                     /note="color: #3366cc"
     misc_feature    complement(159..434)
                     /label=lacZ'
                     /note="dnas_title=lacZ'
                     vntifkey=21"
                     /note="color: #66cc99; direction: LEFT"
     promoter        complement(435..556)
                     /label=lacZ promoter
                     /note="dnas_title=lacZ promoter
                     vntifkey=30"
                     /note="color: #800000; direction: LEFT"
     misc_feature    573..645
                     /label=FUS_A_right
                     /note="dnas_title=FUS_A_right
                     vntifkey=21"
                     /note="color: #a6acb3"
     promoter        646..779
                     /label=Spn promoter
                     /note="dnas_title=Spn promoter
                     vntifkey=30"
                     /note="color: #ffffff"
     misc_feature    780..1790
                     /gene="SpnR"
                     /label=SpnR
                     /note="dnas_title=SpnR
                     invitrogen=2750000
                     vntifkey=21"
                     /note="color: #a6acb3"
     rep_origin      1857..2530
                     /gene="ori"
                     /label=pUC origin
                     /note="dnas_title=pUC origin
                     invitrogen=2530000
                     vntifkey=33"
                     /note="color: #ffff00"
     terminator      2801..2828
                     /label=rrnB T2 transcription terminator
                     /note="dnas_title=rrnB T2 transcription terminator
                     invitrogen=2590000
                     vntifkey=43"
                     /note="color: #ffffff"
     terminator      complement(2960..3003)
                     /gene="T1-T2"
                     /label=rrnB T1 transcription terminator
                     /note="dnas_title=rrnB T1 transcription terminator
                     invitrogen=2570000
                     vntifkey=43"
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac
       61 gacgttgtaa aacgacggcc agtcttaagc gtctccccct gaacctgacc ccggaccaag
      121 tggtggctat cgagaccggc gccgctacag ggcgcgtccc attcgccatt caggctgcgc
      181 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
      241 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
      301 aaaacgacgg ccagtgagcg cgcgtaatac gactcactat agggcgaatt gggtaccggg
      361 ccccccctcg aggtcctcca gcttttgttc cctttagtga gggttaattg cgcgcttggc
      421 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
      481 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
      541 attaattgcg ttgcgctcac tgcccgcttt ccaccggtgg tctcttcgaa acggtgcagc
      601 ggctgttgcc ggtgctgtgc caggaccatg gccgagacgt ctagaccagc caggacagaa
      661 atgcctcgac ttcgctgcta cccaaggttg ccgggtgacg cacaccgtgg aaacggatga
      721 aggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta
      781 tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg
      841 cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct cgggcatcca
      901 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta
      961 cgcagcaggg cagtcgccct aaaacaaagt taaacattat gagggaagcg gtgatcgccg
     1021 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
     1081 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata
     1141 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
     1201 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
     1261 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
     1321 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
     1381 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc
     1441 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
     1501 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
     1561 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
     1621 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
     1681 gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag
     1741 aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa ccctcgagcc
     1801 acccatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg
     1861 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1921 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1981 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     2041 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     2101 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     2161 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     2221 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag
     2281 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     2341 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     2401 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     2461 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     2521 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     2581 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     2641 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     2701 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2761 atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat
     2821 ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc
     2881 gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc
     2941 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag cctttcgttt
     3001 tat
//
