LOCUS       Exported                4492 bp ds-DNA     circular SYN 26-JUN-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pYPQ127 (attL5-attL2)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   UNKNOWN
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jun 26, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
COMMENT     ApEinfo:methylated:1
            ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="synthetic DNA construct"
                     /note="dnas_title=pYPQ127 (attL5-attL2)"
     promoter        5..780
                     /label=3X 35S promoter
                     /note="2X35S"
     misc_feature    793..816
                     /label=FLAG tag
     sig_peptide     820..840
                     /label=NLS-1
     intron          863..947
                     /note="Intron1"
     sig_peptide     948..968
                     /label=NLS-2
     propeptide      969..1100
     misc_feature    1101..1154
                     /label=T2A
     misc_feature    1161..1187
                     /label=HA tag
     sig_peptide     1188..1208
                     /label=NLS-1
     sig_peptide     1249..1269
                     /label=NLS-2
     propeptide      1270..1401
     terminator      1414..1680
                     /label=Nos- terminator
     misc_feature    1700..1799
                     /label=attL2
                     /note="ApEinfo_fwdcolor=#ff0080
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#ff0080
                     dnas_title=attL2
                     vntifkey=21"
     primer_bind     complement(1816..1837)
                     /label=T7
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green
                     dnas_title=T7
                     vntifkey=28"
     primer_bind     complement(1836..1856)
                     /label=M13-rev
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green
                     dnas_title=M13-rev
                     vntifkey=28"
     misc_feature    1934..2067
                     /label=Spn promoter
                     /note="ApEinfo_fwdcolor=#2fec13
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#2fec13
                     dnas_title=Spn promoter
                     vntifkey=21"
     misc_feature    2068..3078
                     /label=SpnR
                     /note="ApEinfo_fwdcolor=#f70938
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#f70938
                     dnas_title=SpnR
                     vntifkey=21"
     misc_feature    3145..3818
                     /label=pUC origin
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green
                     dnas_title=pUC origin
                     vntifkey=21"
     primer_bind     4357..4374
                     /label=M13-fwd
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green
                     dnas_title=M13-fwd
                     vntifkey=28"
     misc_feature    4358..4373
                     /label=M13-F
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=cyan
                     dnas_title=M13-F
                     vntifkey=21"
     misc_feature    4376..4487
                     /label=attL5
                     /note="dnas_title=attL5
                     vntifkey=21"
ORIGIN
        1 gcgaagcttg gcgtgcctgc aggtcaacat ggtggagcac gacacacttg tctactccaa
       61 aaatatcaaa gatacagtct cagaagacca aagggcaatt gagacttttc aacaaagggt
      121 aatatccgga aacctcctcg gattccattg cccagctatc tgtcacttta ttgtgaagat
      181 agtggaaaag gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggccatcgt
      241 tgaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
      301 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg ataacatggt
      361 ggagcacgac acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag
      421 ggcaattgag acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc
      481 agctatctgt cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca
      541 tcattgcgat aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga
      601 tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa
      661 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
      721 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga cctcgactct
      781 ggtaccgata tggactacaa agacgatgac gataaagctc ctaagaaaaa gcgcaaagtc
      841 ccctctagat aacgcaggat ccgtaagttt atcagttaaa tataataaat aaagaagaaa
      901 accaaaaaaa tggctaacta aaacgatggt cttatgattt tatgcagcca aaaaagaaac
      961 gtaaggttga tgctcttgac gattttgacc tcgatatgct cgacgctctt gatgattttg
     1021 atctcgacat gctcgatgca cttgatgact ttgaccttga catgctcgac gcactcgatg
     1081 acttcgacct cgacatgctt gagggcagag gaagtcttct aacatgcggt gacgtggagg
     1141 agaatcccgg ccctatcgat tacccttatg atgtgccaga ctacgctcct aaaaagaagc
     1201 gcaaagttgg cattcacggg gtgccggcta gctaataggc ttagatctcc aaaaaagaaa
     1261 cgtaaggttg atgctcttga cgattttgac ctcgatatgc tcgacgctct tgatgatttt
     1321 gatctcgaca tgctcgatgc acttgatgac tttgaccttg acatgctcga cgcactcgat
     1381 gacttcgacc tcgacatgct ttaataggac gtccgatcgt tcaaacattt ggcaataaag
     1441 tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa
     1501 ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt
     1561 tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc
     1621 aaactaggat aaattatcga aagcggtgtc atctatgtta ctagatcggg aattgatccc
     1681 actagtaagg gcgaattcga cccagctttc ttgtacaaag ttggcattat aaaaaataat
     1741 tgctcatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc
     1801 catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
     1861 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
     1921 tgcctcctct ggaccagcca ggacagaaat gcctcgactt cgctgctgcc caaggttgcc
     1981 gggtgacgca caccgtggaa acggatgaag gcacgaaccc agtggacata agcctgttcg
     2041 gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc
     2101 gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt
     2161 ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
     2221 ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta
     2281 aacatcatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc
     2341 gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg
     2401 gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt
     2461 gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga
     2521 gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg
     2581 tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt
     2641 gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca
     2701 agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct
     2761 gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac
     2821 tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta
     2881 accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc
     2941 cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc
     3001 ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc
     3061 aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa aatcccttaa cgtgagttac
     3121 gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3181 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3241 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3301 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     3361 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3421 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3481 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3541 ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3601 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3661 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     3721 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3781 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     3841 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     3901 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     3961 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     4021 aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctcgccagga agagtttgta
     4081 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt
     4141 atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc
     4201 ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
     4261 cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg
     4321 ttaacgcttg catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag
     4381 ctcgggccca ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga
     4441 tgagcaatgc ttttttataa tgccaacttt gtatacaaaa gttgccccat gg
//
