LOCUS       Exported                3863 bp ds-DNA     circular SYN 30-JUN-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pYPQ131A2.0
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 30, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
COMMENT     LOCUS Exported 3863 bp ds-DNA circular SYN 12-OCT-2016 DEFINITION 
            synthetic circular DNA ACCESSION . VERSION . KEYWORDS pYPQ131A2.0 
            SOURCE synthetic DNA construct ORGANISM synthetic DNA construct 
            REFERENCE 1 (bases 1 to 3863) AUTHORS . TITLE Direct Submission 
            JOURNAL Exported Wednesday, Oct 12, 2016 from SnapGene Viewer 3.2.1 
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1
                     /note="start"
                     /note="color: #0080ff"
     promoter        229..235
                     /label=Tet_ promoter
                     /note="Tet_promoter"
                     /note="color: #ff8080"
     misc_feature    272..1462
                     /note="TetR"
                     /note="color: #0080ff"
     primer_bind     1607..1626
                     /note="pTC14_F2 primer"
                     /note="color: #8080ff; direction: RIGHT"
     misc_feature    1633
                     /note="end"
                     /note="color: #0080ff"
     primer_bind     1703..1724
                     /note="pTC14_R1 primer"
                     /note="color: #8080ff; direction: RIGHT"
     misc_feature    1754..1759
                     /note="BsaI site"
                     /note="color: #0080ff"
     misc_feature    1761..1764
                     /note="4nt"
                     /note="color: #0080ff"
     terminator      1765..2068
                     /label=AtU6 promoter
                     /note="AtU6 promoter"
                     /note="color: #000000"
     -35_signal      2004..2014
                     /note="-35 signal"
                     /note="color: #00ff00; direction: RIGHT"
     TATA_signal     2038..2045
                     /note="TATA"
                     /note="color: #00ff00"
     misc_recomb     2070..2101
                     /note="Guid RNA cloning site"
                     /note="color: #0000ff"
     stem_loop       2102..2244
                     /note="guide RNA scaffold 2.0"
                     /note="color: #00ff00"
     promoter        2245..2610
                     /label=terminator
                     /note="terminator"
                     /note="color: #ff8080"
     misc_feature    complement(2589..2610)
                     /note="gRNA-R"
                     /note="color: #0080ff; direction: LEFT"
     misc_feature    2616..2621
                     /label=BsaI site(1)
                     /note="BsaI site"
                     /note="color: #0080ff"
     misc_feature    2686..3359
                     /note="ori"
                     /note="color: #0080ff"
     primer_bind     3755..3774
                     /note="pTC14_F1 primer"
                     /note="color: #8080ff; direction: RIGHT"
ORIGIN
        1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
       61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      181 aattaattct catgtttgac agcttatcat cgattagctt taatgcggta gtttatcaca
      241 gttaaattgc taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat
      301 cctcggcacc gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg
      361 cctcttgcgg gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc
      421 gctatatgcg ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt
      481 tggccgccgc ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat
      541 ggcgaccaca cccgtcctgt ggattctcta cgccggacgc atcgtggccg gcatcaccgg
      601 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
      661 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
      721 cgggggactg ttgggcgcca tctccttaca tgcaccattc cttgcggcgg cggtgctcaa
      781 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgccg
      841 acccatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
      901 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
      961 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct
     1021 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc
     1081 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg
     1141 ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct
     1201 cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga
     1261 cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat
     1321 tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc
     1381 atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg
     1441 gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact
     1501 ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttga
     1561 tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg
     1621 agaaaataaa atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
     1681 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
     1741 tgcctcctct agaggtctcg ctatagaaat ctcaaaattc cggcagaaca attttgaatc
     1801 tcgatccgta gaaaccagac ggtcattgtt ttagttccac cacgattata tttgaaattt
     1861 acgtgagtgt gagtgagact tgcataagaa aataaaatct ttagttggga aaaaattcaa
     1921 taatataaat gggcttgaga aggaagcgag ggataggcct ttttctaaaa taggcccatt
     1981 taagctatta acaatcttca aaagtaccac agcgcttagg taaagaaagc agctgagttt
     2041 atatatggtt agacacgaag tagtgattgg agacgagatc tagtctgagt cgaccgtctc
     2101 tgttttagag ctaggccaac atgaggatca cccatgtctg cagggcctag caagttaaaa
     2161 taaggctagt ccgttatcaa cttggccaac atgaggatca cccatgtctg cagggccaag
     2221 tggcaccgag tcggtgcttt ttttggcaaa aattttcaga ttttttcttc atctgtagat
     2281 ttctgggttt ttttttccgt ttcgtgaatc ataagtgaag ttttggatgc aaatctgcgc
     2341 gaaaaaagtt ggacctgcaa tgagcttatt tagatagcta agacaaagtg attggtccgt
     2401 tgtttcagtt ctgattgtca gagagtttgt ttcgagtcgg cgacaccaat gcgttttgtt
     2461 aaccagattt cgggtaagaa atgtatcgag agtttgtttc cagacggcta catcattttc
     2521 ttatgaaggg tgaaattaga tagaccaaag attgaaacac aacatttctt tcacaaaaat
     2581 ataataaact tgatagcatt taggatcagc catgggagac cctcgagcca cccatgacca
     2641 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc
     2701 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2761 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2821 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     2881 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2941 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3001 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3061 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3121 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3181 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3241 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3301 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3361 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3421 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     3481 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     3541 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc
     3601 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct
     3661 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
     3721 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca
     3781 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc
     3841 tggcagttcc ctactctcgc gtt
//
