LOCUS       Exported                3566 bp ds-DNA     circular SYN 30-JUN-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pYPQ131C2.0
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 30, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
COMMENT     LOCUS pHD1 3087 bp DNA circular 10-DEC-2010 SOURCE ORGANISM COMMENT 
            This file is created by Vector NTI http://www.invitrogen.com/ 
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FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1
                     /label=start
                     /note="color: #0080ff"
     promoter        229..235
                     /label=Tet_ promoter
                     /note="color: #ff8080"
     misc_feature    272..1462
                     /label=TetR
                     /note="color: #0080ff"
     primer_bind     1607..1626
                     /label=pTC14_F2 primer
                     /note="color: #8080ff; direction: RIGHT"
     misc_feature    1633
                     /label=end
                     /note="color: #0080ff"
     primer_bind     1703..1724
                     /label=pTC14_R1 primer
                     /note="color: #8080ff; direction: RIGHT"
     misc_feature    1754..1759
                     /note="BsaI site"
                     /note="color: #0080ff"
     misc_feature    1761..1764
                     /note="4nt"
                     /note="color: #0080ff"
     promoter        1765..2063
                     /label=OsU6-2 promoter
                     /note="OsU6-2 promoter"
                     /note="color: #ff8080"
     misc_feature    1765..1787
                     /label=GGTACCGAGCTCGGATCCACTAG
                     /note="pYPQ13xC-F"
                     /note="color: #50ff50"
     TATA_signal     2037..2040
                     /note="TATA box"
                     /note="color: #00ff00"
     misc_structure  2065..2096
                     /note="guide RNA cloning site"
                     /note="color: #0080ff"
     stem_loop       2097..2239
                     /note="guide RNA scaffold 2.0"
                     /note="color: #00ff00"
     terminator      2240..2313
                     /label=OsU6-2 terminator
                     /note="OsU6-2 terminator"
                     /note="color: #000000"
     misc_feature    complement(2291..2313)
                     /label=AAGCTTATCGATACCCTCGACCT
                     /note="pYPQ13xC-R"
                     /note="color: #50ff50; direction: LEFT"
     misc_feature    2319..2324
                     /note="BsaI site"
                     /note="color: #0080ff"
     misc_feature    2389..3062
                     /label=ori
                     /note="color: #0080ff"
     primer_bind     3458..3477
                     /label=pTC14_F1 primer
                     /note="color: #8080ff; direction: RIGHT"
ORIGIN
        1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
       61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      181 aattaattct catgtttgac agcttatcat cgattagctt taatgcggta gtttatcaca
      241 gttaaattgc taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat
      301 cctcggcacc gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg
      361 cctcttgcgg gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc
      421 gctatatgcg ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt
      481 tggccgccgc ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat
      541 ggcgaccaca cccgtcctgt ggattctcta cgccggacgc atcgtggccg gcatcaccgg
      601 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
      661 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
      721 cgggggactg ttgggcgcca tctccttaca tgcaccattc cttgcggcgg cggtgctcaa
      781 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgccg
      841 acccatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
      901 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
      961 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct
     1021 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc
     1081 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg
     1141 ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct
     1201 cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga
     1261 cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat
     1321 tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc
     1381 atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg
     1441 gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact
     1501 ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttga
     1561 tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg
     1621 agaaaataaa atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
     1681 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
     1741 tgcctcctct agaggtctcg ctatggtacc gagctcggat ccactagtaa cggccgccag
     1801 tgtgctggaa ttgcccttgg atcatgaacc aacggcctgg ctgtatttgg tggttgtgta
     1861 gggagatggg gagaagaaaa gcccgattct cttcgctgtg atgggctgga tgcatgcggg
     1921 ggagcgggag gcccaagtac gtgcacggtg agcggcccac agggcgagtg tgagcgcgag
     1981 aggcgggagg aacagtttag taccacattg cccagctaac tcgaacgcga ccaacttata
     2041 aacccgcgcg ctgtcgcttg tgtggagacg agatctagtc tgagtcgacc gtctctgttt
     2101 tagagctagg ccaacatgag gatcacccat gtctgcaggg cctagcaagt taaaataagg
     2161 ctagtccgtt atcaacttgg ccaacatgag gatcacccat gtctgcaggg ccaagtggca
     2221 ccgagtcggt gctttttttg tcccttcgaa gggcaattct gcagatatcc atcacactgg
     2281 cggccgctcg aggtcgaggg tatcgataag cttcatggga gaccctcgag ccacccatga
     2341 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag
     2401 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2461 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2521 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     2581 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     2641 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     2701 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2761 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2821 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2881 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2941 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     3001 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     3061 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     3121 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     3181 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     3241 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt
     3301 accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct
     3361 gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt
     3421 gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg
     3481 acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat
     3541 gcctggcagt tccctactct cgcgtt
//
