LOCUS Exported 3694 bp ds-DNA circular SYN 30-JUN-2017 DEFINITION . ACCESSION . VERSION . KEYWORDS pYPQ132B2.0 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3694) AUTHORS . TITLE Direct Submission JOURNAL Exported Friday, Jun 30, 2017 from SnapGene 3.3.4 http://www.snapgene.com COMMENT LOCUS pHD2 3111 bp DNA circular 1-DEC-2010 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|562784720| COMMENT VNTDBDATE|572462821| COMMENT LSOWNER| COMMENT VNTNAME|pHD2| COMMENT VNTAUTHORNAME|Demo User| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc ""pHD2"" 0 3111 COMMENT (CDBMol 0 0 1 1 1 0 0 0 0 """" """" 0 0 0 0 (CObList) (CObList) (CObList) COMMENT (CObList) -1 """") COMMENT (CDocSetData 1 1 0 0 0 0 ""MAIN"" 1 1 1 1 0 0 1 1 1 0 10 10 4294967295 50 0 COMMENT 1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) COMMENT (CStringList ""ApaLI"" ""AvaI"" ""BamHI"" ""ClaI"" ""EcoRI"" ""HindIII"" ""NcoI"" COMMENT ""PstI"" ""SmaI"" ""XmaI"" ""BsmBI"" ""XbaI"" ""XhoI"") (CStringList ""atg"" ""gtg"") COMMENT (CStringList ""taa"" ""tga"" ""tag"") (CObList) 1 ""{(0,1),2}"" 0 0 """" 0 COMMENT 4294967295 0 0 0 0 0 0 0 ""MAIN"" 0 0 30 0 COMMENT (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) COMMENT (CMolPar 0 0 0 0 0 1 3111 0 0 0 0 0 0 0 0) (CStringList) (CStringList) COMMENT (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList #0=(CRSite (CStringList) ""ApaLI"" ""gtgcac"" 2 0 1 2291 0 0 """") COMMENT #1=(CRSite (CStringList) ""AvaI"" ""cycgrg"" 2 0 1 1871 0 0 """") COMMENT #2=(CRSite (CStringList) ""BamHI"" ""ggatcc"" 2 0 0 0 """") COMMENT #3=(CRSite (CStringList) ""ClaI"" ""atcgat"" 3 0 1 211 0 0 """") COMMENT #4=(CRSite (CStringList) ""EcoRI"" ""gaattc"" 2 0 0 0 """") COMMENT #5=(CRSite (CStringList) ""HindIII"" ""aagctt"" 2 0 0 0 """") COMMENT #6=(CRSite (CStringList) ""NcoI"" ""ccatgg"" 2 0 1 1761 0 0 """") COMMENT #7=(CRSite (CStringList) ""PstI"" ""ctgcag"" 6 0 0 0 """") COMMENT #8=(CRSite (CStringList) ""SmaI"" ""cccggg"" 4 0 0 0 """") COMMENT #9=(CRSite (CStringList) ""XmaI"" ""cccggg"" 2 0 0 0 """") COMMENT #10=(CRSite (CStringList) ""BsmBI"" ""cgtctc"" 8 12 0 0 """") COMMENT #11=(CRSite (CStringList) ""XbaI"" ""tctaga"" 2 0 1 1749 0 0 """") COMMENT #12=(CRSite (CStringList) ""XhoI"" ""ctcgag"" 2 0 1 1871 0 0 """")) COMMENT (CObList COMMENT #13=(CFSignal (CObList) ""TetR"" 21 0 0 272 1462 0 (CStringList) COMMENT (CStringList) 1 1 1 1 """") COMMENT #14=(CFSignal (CObList) ""Tet_promoter"" 30 0 0 229 235 0 (CStringList) COMMENT (CStringList) 1 1 1 1 """") COMMENT #15=(CFSignal (CObList) ""pTC14_F1 primer"" 28 0 0 3003 3022 0 COMMENT (CStringList) (CStringList) 1 1 1 1 """") COMMENT #16=(CFSignal (CObList) ""end"" 21 0 0 1633 1633 0 (CStringList) COMMENT (CStringList) 1 1 1 1 """") COMMENT #17=(CFSignal (CObList) ""pTC14_R1 primer"" 28 0 0 1703 1724 0 COMMENT (CStringList) (CStringList) 1 1 1 1 """") COMMENT #18=(CFSignal (CObList) ""start"" 21 0 0 1 1 0 (CStringList) (CStringList) COMMENT 1 1 1 1 """") COMMENT #19=(CFSignal (CObList) ""pTC14_F2 primer"" 28 0 0 1607 1626 0 COMMENT (CStringList) (CStringList) 1 1 1 1 """") COMMENT #20=(CFSignal (CObList) ""HD2"" 21 0 0 1754 1869 0 (CStringList) COMMENT (CStringList) 1 1 1 1 """") COMMENT #21=(CFSignal (CObList) ""ori"" 21 0 0 1934 2607 0 (CStringList) COMMENT (CStringList) 1 1 1 1 """") COMMENT #22=(CFSignal (CObList) ""FRAG HD-2"" 21 0 0 1765 1862 0 (CStringList) COMMENT (CStringList) 1 1 1 1 """")) (CObList) (CObList) (CObList) (CObList) COMMENT (CObList) (CObList) COMMENT (CTextView 0 COMMENT #23=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #24=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) ""pHD2"" 2) 5 """" 0 4) COMMENT #25=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) ""DNA 'pHD2'"" COMMENT 1) COMMENT #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT ""Local object"" 1) COMMENT #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT ""Created: 08/17/10 05:05PM"" 1) COMMENT #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT ""Last Modified: 12/01/10 05:27PM"" 1) COMMENT #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT ""length: 3111 bp"" 1) COMMENT #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT ""storage type: Basic"" 1) COMMENT #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT ""form: Circular"" 1))) ""General Description"") COMMENT #33=(CFolderPar COMMENT (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) (CObjectList)) COMMENT ""Standard Fields"") COMMENT #34=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) ""Comments"" 2) 1 """" COMMENT 0 0) COMMENT #35=(CFolderPar COMMENT (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) COMMENT ""Annotations"") COMMENT #36=(CFolderPar COMMENT (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #37=(CFolderPar COMMENT (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #38=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1 (3 #18# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #39=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT ""Start: 1 End: 1 "" 1))) ""start"") COMMENT #40=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 272 (3 #13# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #41=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT ""Start: 272 End: 1462"" 1))) ""TetR"") COMMENT #42=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1633 (3 #16# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #43=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT ""Start: 1633 End: 1633"" 1))) ""end"") COMMENT #44=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1754 (3 #20# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #45=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT ""Start: 1754 End: 1869"" 1))) ""HD2"") COMMENT #46=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1765 (3 #22# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #47=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT ""Start: 1765 End: 1862"" 1))) COMMENT ""FRAG HD-2"") COMMENT #48=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1934 (3 #21# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #49=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT ""Start: 1934 End: 2607"" 1))) ""ori""))) COMMENT ""Misc. Feature (6 total)"") COMMENT #50=(CFolderPar COMMENT (CGroupPar (CParagraph 28 (7 28 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #51=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1607 (3 #19# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #52=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT ""Start: 1607 End: 1626"" 1))) COMMENT ""pTC14_F2 primer"") COMMENT #53=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1703 (3 #17# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #54=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT ""Start: 1703 End: 1724"" 1))) COMMENT ""pTC14_R1 primer"") COMMENT #55=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3003 (3 #15# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #56=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT ""Start: 3003 End: 3022"" 1))) COMMENT ""pTC14_F1 primer""))) COMMENT ""Primer Binding Site (3 total)"") COMMENT #57=(CFolderPar COMMENT (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #58=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 229 (3 #14# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #59=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT ""Start: 229 End: 235 "" 1))) COMMENT ""Tet_promoter""))) COMMENT ""Promoter Prokaryotic (1 total)""))) ""Feature Map"") COMMENT #60=(CFolderPar COMMENT (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #61=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 74651912 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #62=(CGroupPar COMMENT (CParagraph 0 (10 #0# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #63=(CLinePar COMMENT (CParagraph 0 (1 #0# 1) 1 2 2 0 191) COMMENT "" N1: 2291 "" 1))))) ""ApaLI: 1 site"") 1 5 COMMENT ""GTGCAC"" ""CACGTG"") COMMENT #64=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 74651880 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #65=(CGroupPar COMMENT (CParagraph 0 (10 #1# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #66=(CLinePar COMMENT (CParagraph 0 (1 #1# 1) 1 2 2 0 191) COMMENT "" N1: 1871 "" 1))))) ""AvaI: 1 site"") 1 5 COMMENT ""CYCGRG"" ""GRGCYC"") COMMENT #67=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 74649800 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) ""BamHI: 0 sites"") 1 5 ""GGATCC"" COMMENT ""CCTAGG"") COMMENT #68=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 74646632 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) ""BsmBI: 0 sites"") 7 11 ""CGTCTCNNNNNN"" COMMENT ""GCAGAGNNNNNN"") COMMENT #69=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 74649768 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #70=(CGroupPar COMMENT (CParagraph 0 (10 #3# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #71=(CLinePar COMMENT (CParagraph 0 (1 #3# 1) 1 2 2 0 191) COMMENT "" N1: 211 "" 1))))) ""ClaI: 1 site"") 2 4 COMMENT ""ATCGAT"" ""TAGCTA"") COMMENT #72=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 74649672 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) ""EcoRI: 0 sites"") 1 5 ""GAATTC"" COMMENT ""CTTAAG"") COMMENT #73=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 74649640 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) ""HindIII: 0 sites"") 1 5 ""AAGCTT"" COMMENT ""TTCGAA"") COMMENT #74=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 74649608 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #75=(CGroupPar COMMENT (CParagraph 0 (10 #6# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #76=(CLinePar COMMENT (CParagraph 0 (1 #6# 1) 1 2 2 0 191) COMMENT "" N1: 1761 "" 1))))) ""NcoI: 1 site"") 1 5 COMMENT ""CCATGG"" ""GGTACC"") COMMENT #77=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 74649576 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) ""PstI: 0 sites"") 5 1 ""CTGCAG"" COMMENT ""GACGTC"") COMMENT #78=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 74649416 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) ""SmaI: 0 sites"") 3 3 ""CCCGGG"" COMMENT ""GGGCCC"") COMMENT #79=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 74646600 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #80=(CGroupPar COMMENT (CParagraph 0 (10 #11# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #81=(CLinePar COMMENT (CParagraph 0 (1 #11# 1) 1 2 2 0 191) COMMENT "" N1: 1749 "" 1))))) ""XbaI: 1 site"") 1 5 COMMENT ""TCTAGA"" ""AGATCT"") COMMENT #82=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 74646568 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #83=(CGroupPar COMMENT (CParagraph 0 (10 #12# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #84=(CLinePar COMMENT (CParagraph 0 (1 #12# 1) 1 2 2 0 191) COMMENT "" N1: 1871 "" 1))))) ""XhoI: 1 site"") 1 5 COMMENT ""CTCGAG"" ""GAGCTC"") COMMENT #85=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 74649384 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) ""XmaI: 0 sites"") 1 5 ""CCCGGG"" COMMENT ""GGGCCC""))) ""Restriction/Methylation Map"")))) COMMENT (CGraphView COMMENT (CWStyleSheet COMMENT (CObjectList COMMENT #86=(CWidgetStyle ""RSite Label"" 1 (LOGPEN 0 0 13408563) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 ""Georgia"") 0.555556 0 1 5 COMMENT ""@N (@S)"" 0) COMMENT #87=(CWidgetStyle ""Signal Label"" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 ""Arial"") 0.666667 0 1 1 COMMENT ""@N"" 0) COMMENT #88=(CWidgetStyle ""Molecule Label 2"" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 ""Georgia"") 0.555556 0 1 16 COMMENT ""@L bp"" 0) COMMENT #89=(CWidgetStyle ""Molecule Label 1"" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 ""Verdana"") 0.833333 0 1 1 COMMENT ""@N"" 0) COMMENT #90=(CWidgetStyle ""Shape 3"" 1 (LOGPEN 0 0 3355545) 1 1 COMMENT (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #91=(CWidgetStyle ""Shape 1"" 1 (LOGPEN 0 0 6723840) 1 1 COMMENT (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #92=(CWidgetStyle ""Axis"" 1 (LOGPEN 0 0 10079436) 2 1 COMMENT (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) COMMENT #93=(CWidgetStyle ""Line 2"" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #94=(CWidgetStyle ""RSite"" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #95=(CWidgetStyle ""Short Signal"" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #96=(CWidgetStyle ""Uniq RSite Label"" 1 (LOGPEN 0 0 153) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 ""Georgia"") 0.555556 128 1 5 COMMENT ""@N (@S)"" 0) COMMENT #97=(CWidgetStyle ""Vanilla"" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 ""Times New Roman"") 0.8 0 1 COMMENT 2 ""?"" 0) COMMENT #98=(CWidgetStyle ""Mark 1"" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 ""Windings"") 0.7 0 1 2 ""?"" 0) COMMENT #99=(CWidgetStyle ""Motif Label"" 1 (LOGPEN 0 0 16744512) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 ""Arial"") 0.611111 8388608 1 COMMENT 65535 ""@N (@H)"" 0) COMMENT #100=(CWidgetStyle ""Fragment Label 2"" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 ""Courier New"") 1.05 0 1 48 COMMENT ""@F bp (molecule @L bp)"" 0) COMMENT #101=(CWidgetStyle ""Fragment Label 1"" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 ""Arial"") 0.91 0 1 1 COMMENT ""Fragment of @N"" 0) COMMENT #102=(CWidgetStyle ""Shape 4"" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 2 8388608 5) 0 0 0) COMMENT #103=(CWidgetStyle ""Shape 2"" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 COMMENT 0 0) COMMENT #104=(CWidgetStyle ""Shape 0"" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 COMMENT 0) COMMENT #105=(CWidgetStyle ""ORF"" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 COMMENT (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) COMMENT #106=(CWidgetStyle ""Line 4"" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) COMMENT #107=(CWidgetStyle ""Line 3"" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) COMMENT #108=(CWidgetStyle ""Line 1"" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) COMMENT #109=(CWidgetStyle ""Short Promoter"" 1 (LOGPEN 0 0 128) 6 0 0 0 0) COMMENT #110=(CWidgetStyle ""Motif"" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #111=(CWidgetStyle ""Line 0"" 1 (LOGPEN 0 0 0) 8 0 0 0 0) COMMENT #112=(CWidgetStyle ""Void"" 0 0 0 0 0) COMMENT #113=(CWidgetStyle ""General Label"" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 ""Times New Roman"") 0.91 0 COMMENT 1 3 ""@T @N "" 0) COMMENT #114=(CWidgetStyle ""Position"" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #115=(CWidgetStyle ""Annotation"" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 ""Times New Roman"") 0.91 0 COMMENT 0 0) COMMENT #116=(CWidgetStyle ""Position Label"" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 ""Arial"") 0.63 8388608 1 1 COMMENT ""@N"" 0) COMMENT #117=(CWidgetStyle ""Range"" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 0 0 0) COMMENT #118=(CWidgetStyle ""Range Label"" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 ""Arial"") 0.63 8388608 1 1 COMMENT ""@N"" 0) COMMENT #119=(CWidgetStyle ""ORF Label"" 1 (LOGPEN 0 0 49216) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 ""Times New Roman"") COMMENT 0.611111 0 1 65535 ""@N"" 0) COMMENT #120=(CWidgetStyle ""CDS Label"" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 ""Arial"") 0.555556 255 1 1 COMMENT ""@N"" 0) COMMENT #121=(CWidgetStyle ""Shape 5"" 1 (LOGPEN 0 0 0) 3 1 COMMENT (LOGBRUSH 0 16777113 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 ""Arial"") 0.9 0 0 1 COMMENT (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #122=(CWidgetStyle ""CDS"" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 COMMENT 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #123=(CWidgetStyle ""Label 2"" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 ""Arial"") 0.944444 8388608 COMMENT 1 1 ""@N"" 0) COMMENT #124=(CWidgetStyle ""Label 3"" 1 (LOGPEN 0 0 8421376) 1 0 1 COMMENT (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 ""Arial"") 0.833333 255 1 COMMENT 5 ""@N (@S)"" 0) COMMENT #125=(CWidgetStyle ""Label 4"" 1 (LOGPEN 0 0 8437824) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 ""Arial"") 0.722222 0 1 5 COMMENT ""@N (@S)"" 0)) 0.164644 1.74233 0.164644 2.53336 COMMENT (70 (CShapeMapEntry 0 ""Unique RSite"" 1 ""Uniq RSite Label"") 67 COMMENT (CShapeMapEntry 0 ""ORF"" 0 ""ORF Label"")) 40.0378 40.0378 39 39 0.1 -365) COMMENT 1 1 0 1 1 COMMENT (mapper: 19.9966 -16.2152 40.0378 40.0378 0.01 10 -1 3111 3111 1 0 0) COMMENT #126=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 1867275361 100) COMMENT (CObjectList COMMENT #127=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList COMMENT #128=(CAxis COMMENT (CWideLine (CWidget 0 (0 0) 1 2 0 0 #92# 49923276 0) COMMENT (LOGPEN 0 0 10079436) 2 (LOGBRUSH 0 13434879 0) 1 COMMENT 6.2762 6.2742 1 0.0214037) 0.201645) COMMENT #129=(CLabel COMMENT (CWidget 1001 (0 0) 1 2 0 0 #89# -2101249 100) COMMENT (LOGPEN 4293261055 -2298117 470491469) 1790693347 COMMENT (LOGFONT 58 22 0 0 400 0 0 0 0 3 2 1 34 ""Verdana"") COMMENT 1.74233 0.833333 0 ""pHD2"" ""@N"" 1 0 0.871165 0 -10 COMMENT 3.09707 1.39868 Nil) COMMENT #130=(CLabel (CWidget 1002 (0 0) 1 2 0 0 #88# 8 100) COMMENT (LOGPEN 8 8 8) 8 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 ""Georgia"") COMMENT 1.74233 0.555556 0 ""3111 bp"" ""@L bp"" 16 0 -0.871165 COMMENT 0 -10.8 2.17295 0.799245 Nil)) (CObjectList)) COMMENT #131=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList COMMENT #132=(CGroupWidget (CWidget 21 (7 21 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList COMMENT #133=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #13# 0) 1 2 0 0 #91# COMMENT 1667321128 100) (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 1 3.32513 5.72673 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #134=(CLabel (CWidget 0 (0 0) 1 2 0 0 #87# 8 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT ""Arial"") 1.74233 0.666667 0 ""TetR"" ""@N"" 1 COMMENT 14.4009 -1.74233 0 -10 1.94816 1.04901 #133#) COMMENT #135=(CScratch COMMENT (CWidget 0 (3 #16# 0) 1 2 0 0 #95# 1818324545 COMMENT 100) (LOGPEN 0 8 13395507) 10 1 2.98233 1.9 COMMENT 0.082322 1) COMMENT #136=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #87# 268697606 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT ""Arial"") 1.74233 0.666667 0 ""end"" ""@N"" 1 COMMENT -3.87923 -15.681 0 -10 1.67342 1.04901 #135#) COMMENT #137=(CScratch COMMENT (CWidget 0 (3 #18# 0) 1 2 0 0 #95# 0 100) COMMENT (LOGPEN 0 8 13395507) 10 1 6.27319 1.9 COMMENT 0.082322 1) COMMENT #138=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #87# 268697662 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT ""Arial"") 1.74233 0.666667 0 ""start"" ""@N"" 1 COMMENT 1.96079 13.9386 0 -10 2.04806 1.04901 #137#) COMMENT #139=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #20# 0) 1 2 0 0 #91# -2894893 COMMENT 100) (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 1 2.50443 2.73834 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #140=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #87# 268697606 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT ""Arial"") 1.74233 0.666667 0 ""HD2"" ""@N"" 1 COMMENT -11.5828 -8.71165 0 -10 1.87323 1.04901 #139#) COMMENT #141=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #21# 0) 1 2 0 0 #91# -2829100 COMMENT 100) (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 1 1.01629 2.37538 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #142=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #87# 268697634 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT ""Arial"") 1.74233 0.666667 0 ""ori"" ""@N"" 1 COMMENT -14.0084 -1.74233 0 -10 1.24882 1.04901 #141#) COMMENT #143=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #22# 0) 1 2 0 0 #91# -2829100 COMMENT 100) (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.835356 2.51855 COMMENT 2.71616 1 0.082322) 0.8 1.8 0) COMMENT #144=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #87# 268697662 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT ""Arial"") 1.74233 0.666667 0 ""FRAG HD-2"" ""@N"" COMMENT 1 -14.2164 -6.96932 0 -10 4.84542 1.04901 COMMENT #143#)) (CObjectList)) COMMENT #145=(CGroupWidget (CWidget 28 (7 28 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList COMMENT #146=(CScratch COMMENT (CWidget 0 (3 #15# 0) 1 2 0 0 #108# 11 100) COMMENT (LOGPEN 0 0 16711680) 1 1 0.219793 1.9 COMMENT 0.082322 1) COMMENT #147=(CLabel (CWidget 0 (0 0) 1 2 0 0 #87# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT ""Arial"") 1.74233 0.666667 0 COMMENT ""pTC14 F1 primer"" ""@N"" 1 -7.31285 13.9386 0 COMMENT -10 7.14325 1.04901 #146#) COMMENT #148=(CScratch COMMENT (CWidget 0 (3 #17# 0) 1 2 0 0 #108# 0 100) COMMENT (LOGPEN 0 0 16711680) 1 1 2.84118 1.9 COMMENT 0.082322 1) COMMENT #149=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #87# 268697634 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT ""Arial"") 1.74233 0.666667 0 COMMENT ""pTC14 R1 primer"" ""@N"" 1 -8.38711 -13.9386 0 COMMENT -10 7.24316 1.04901 #148#) COMMENT #150=(CScratch COMMENT (CWidget 0 (3 #19# 0) 1 2 0 0 #108# 0 100) COMMENT (LOGPEN 0 0 16711680) 1 1 3.03476 1.9 COMMENT 0.082322 1) COMMENT #151=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #87# 268697690 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT ""Arial"") 1.74233 0.666667 0 COMMENT ""pTC14 F2 primer"" ""@N"" 1 -5.99965 -17.4233 0 COMMENT -10 7.14325 1.04901 #150#)) (CObjectList)) COMMENT #152=(CGroupWidget (CWidget 30 (7 30 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList COMMENT #153=(CPromoterArrow COMMENT (CLine COMMENT (CWidget 0 (3 #14# 0) 1 2 0 0 #109# 0 100) COMMENT (LOGPEN 0 4 128) 6 1.16464 5.72617 5.81343) COMMENT 0.4 0.082322 1.08464 1.8 1.8 0) COMMENT #154=(CLabel (CWidget 0 (0 0) 1 2 0 0 #87# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT ""Arial"") 1.74233 0.666667 0 ""Tet promoter"" COMMENT ""@N"" 1 11.867 10.454 0 -10 5.46983 1.04901 COMMENT #153#)) (CObjectList))) (CObjectList)) COMMENT #155=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList COMMENT #156=(CGroupWidget COMMENT (CWidget 1 (10 #0# 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList COMMENT #157=(CScratch COMMENT (CWidget 1 (1 #0# 1) 1 2 0 0 #94# 0 100) COMMENT (LOGPEN 0 6 10053171) 8 1 1.65551 1.9 COMMENT 0.082322 1) COMMENT #158=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #96# 268697690 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT ""Georgia"") 1.74233 0.555556 128 COMMENT ""{\\i Apa}LI (2291)"" ""@N (@S)"" 5 -16.1146 0 0 COMMENT -10 4.24599 0.799245 #157#)) (CObjectList)) COMMENT #159=(CGroupWidget COMMENT (CWidget 1 (10 #1# 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList COMMENT #160=(CScratch COMMENT (CWidget 1 (1 #1# 1) 1 2 0 0 #94# 0 100) COMMENT (LOGPEN 0 6 10053171) 8 1 2.50242 1.9 COMMENT 0.082322 1) COMMENT #161=(CLabel (CWidget 0 (0 0) 1 2 0 0 #96# 0 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT ""Georgia"") 1.74233 0.555556 128 COMMENT ""{\\i Ava}I (1871)"" ""@N (@S)"" 5 -14.9734 COMMENT -5.22699 0 -10 3.62158 0.799245 #160#)) COMMENT (CObjectList)) COMMENT #162=(CGroupWidget COMMENT (CWidget 1 (10 #3# 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList COMMENT #163=(CScratch COMMENT (CWidget 1 (1 #3# 1) 1 2 0 0 #94# 0 100) COMMENT (LOGPEN 0 6 10053171) 8 1 5.84973 1.9 COMMENT 0.082322 1) COMMENT #164=(CLabel (CWidget 0 (0 0) 1 2 0 0 #96# 0 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT ""Georgia"") 1.74233 0.555556 128 COMMENT ""{\\i Cla}I (211)"" ""@N (@S)"" 5 8.31175 COMMENT 12.1963 0 -10 2.99717 0.799245 #163#)) COMMENT (CObjectList)) COMMENT #165=(CGroupWidget COMMENT (CWidget 1 (10 #6# 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList COMMENT #166=(CScratch COMMENT (CWidget 1 (1 #6# 1) 1 2 0 0 #94# 0 100) COMMENT (LOGPEN 0 6 10053171) 8 1 2.72423 1.9 COMMENT 0.082322 1) COMMENT #167=(CLabel (CWidget 0 (0 0) 1 2 0 0 #96# 0 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT ""Georgia"") 1.74233 0.555556 128 COMMENT ""{\\i Nco}I (1761)"" ""@N (@S)"" 5 -11.3879 COMMENT -10.454 0 -10 3.62158 0.799245 #166#)) COMMENT (CObjectList)) COMMENT #168=(CGroupWidget COMMENT (CWidget 1 (10 #11# 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList COMMENT #169=(CScratch COMMENT (CWidget 1 (1 #11# 1) 1 2 0 0 #94# 0 100) COMMENT (LOGPEN 0 6 10053171) 8 1 2.74843 1.9 COMMENT 0.082322 1) COMMENT #170=(CLabel (CWidget 0 (0 0) 1 2 0 0 #96# 0 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT ""Georgia"") 1.74233 0.555556 128 COMMENT ""{\\i Xba}I (1749)"" ""@N (@S)"" 5 -8.76475 COMMENT -12.1963 0 -10 3.89632 0.799245 #169#)) COMMENT (CObjectList)) COMMENT #171=(CGroupWidget COMMENT (CWidget 1 (10 #12# 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList COMMENT #172=(CScratch COMMENT (CWidget 1 (1 #12# 1) 1 2 0 0 #94# 0 100) COMMENT (LOGPEN 0 6 10053171) 8 1 2.50242 1.9 COMMENT 0.082322 1) COMMENT #173=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #96# 2082537484 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT ""Georgia"") 1.74233 0.555556 128 COMMENT ""{\\i Xho}I (1871)"" ""@N (@S)"" 5 -15.4459 COMMENT -3.48466 0 -10 3.72148 0.799245 #172#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #174=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList) (CObjectList)) COMMENT #175=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil 0 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 41825552 COMMENT (CStringList) 0 1521479129 (CObList))) FEATURES Location/Qualifiers source 1..3694 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1 /label=start /note="color: #0080ff" promoter 229..235 /label=Tet_ promoter /note="color: #ff8080" misc_feature 272..1462 /label=TetR /note="color: #0080ff" primer_bind 1607..1626 /label=pTC14_F2 primer /note="color: #8080ff; direction: RIGHT" misc_feature 1633 /label=end /note="color: #0080ff" primer_bind 1703..1724 /label=pTC14_R1 primer /note="color: #8080ff; direction: RIGHT" misc_feature 1754..1759 /note="BsaI site" /note="color: #0080ff" misc_feature 1761..1764 /note="4nt" /note="color: #0080ff" promoter 1765..2090 /label=AtU3 promoter /note="AtU3 promoter" /note="color: #ff8080" modified_base 1840 /note="G to c" /note="color: #000000; direction: RIGHT" misc_feature 2026..2035 /note="USE" /note="color: #0080ff" TATA_signal 2060..2067 /note="TATA box" /note="color: #00ff00" misc_feature 2091 /note="first necletide transcript" /note="color: #0080ff" misc_structure 2092..2123 /note="guide RNA cloning site" /note="color: #0080ff" stem_loop 2124..2266 /note="guide RNA scaffold 2.0" /note="color: #00ff00" terminator 2267..2441 /label=AtU3 terminator /note="AtU3 terminator" /note="color: #000000" misc_feature 2447..2452 /note="BsaI site" /note="color: #0080ff" misc_feature 2517..3190 /label=ori /note="color: #0080ff" primer_bind 3586..3605 /label=pTC14_F1 primer /note="color: #8080ff; direction: RIGHT" ORIGIN 1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc 61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 181 aattaattct catgtttgac agcttatcat cgattagctt taatgcggta gtttatcaca 241 gttaaattgc taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat 301 cctcggcacc gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg 361 cctcttgcgg gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc 421 gctatatgcg ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt 481 tggccgccgc ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat 541 ggcgaccaca cccgtcctgt ggattctcta cgccggacgc atcgtggccg gcatcaccgg 601 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc 661 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc 721 cgggggactg ttgggcgcca tctccttaca tgcaccattc cttgcggcgg cggtgctcaa 781 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgccg 841 acccatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac 901 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc 961 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct 1021 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc 1081 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg 1141 ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct 1201 cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga 1261 cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat 1321 tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc 1381 atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg 1441 gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact 1501 ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttga 1561 tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg 1621 agaaaataaa atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag 1681 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca 1741 tgcctcctct agaggtctcc catgtttact ttaaattttt cttatggctc agcctgtgat 1801 ggataactga atcaaacaaa tggcgtctgg gtttaagaac atctgttttg gctatgttgg 1861 acgaaacaag tgaactttta ggatcaactt ccgtttatat acggagctta tatcgagcaa 1921 taagataagt gggcttttta tgtaatttaa tgggctatcg tccatatatt cactaatacc 1981 catgcccagt acccatgtat gcgtttcata taagctccta atttctccca catcgctcaa 2041 atctaaacaa atcttgttgt atatataaca ctgagggagc accattggtc agagacgaga 2101 tctagtctga gtcgaccgtc tctgttttag agctaggcca acatgaggat cacccatgtc 2161 tgcagggcct agcaagttaa aataaggcta gtccgttatc aacttggcca acatgaggat 2221 cacccatgtc tgcagggcca agtggcaccg agtcggtgct tttttttccc tttccttttt 2281 tctttttttt gccataaact taaatttgta tatcgatcat tgtagatatt gaaaacctag 2341 aacaaaccaa catccatgtg aatgtctttc atgactgatt tagagataat tcttgaattt 2401 tggaactaga atctataatg agcctaaatt aaaacattgt gggaccgaga ccctcgagcc 2461 acccatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg 2521 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 2581 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 2641 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt 2701 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 2761 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 2821 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 2881 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 2941 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 3001 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 3061 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 3121 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 3181 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 3241 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 3301 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 3361 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 3421 atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat 3481 ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc 3541 gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc 3601 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag cctttcgttt 3661 tatttgatgc ctggcagttc cctactctcg cgtt //