LOCUS Exported 3863 bp ds-DNA circular SYN 30-JUN-2017 DEFINITION . ACCESSION . VERSION . KEYWORDS pYPQ133A2.0 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3863) AUTHORS . TITLE Direct Submission JOURNAL Exported Friday, Jun 30, 2017 from SnapGene 3.3.4 http://www.snapgene.com COMMENT LOCUS Exported 3863 bp ds-DNA circular SYN 12-OCT-2016 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pYPQ133A2.0 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3863) AUTHORS . TITLE Direct Submission JOURNAL Exported Wednesday, Oct 12, 2016 from SnapGene Viewer 3.2.1 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3863 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 229..235 /note="Tet Promoter" /note="color: #0080ff" misc_feature 272..1462 /note="TetR" /note="color: #0080ff" misc_feature 1607..1626 /note="pTC14_F2 Primer" /note="color: #0080ff" primer_bind complement(1636..1657) /note="T7" /note="color: #8080ff; direction: LEFT" primer_bind complement(1656..1676) /note="M13-rev" /note="color: #8080ff; direction: LEFT" misc_feature 1703..1724 /note="pTC14_R1 Primer" /note="color: #0080ff" misc_feature complement(1754..1759) /note="BsaI Site(1)" /note="color: #0080ff; direction: LEFT" terminator 1765..2068 /label=AtU6 promoter /note="AtU6 promoter" /note="color: #000000" misc_feature 1765..1787 /note="forward temp" /note="color: #0080ff" -35_signal 2004..2014 /note="-35 signal" /note="color: #00ff00; direction: RIGHT" TATA_signal 2038..2045 /note="TATA" /note="color: #00ff00" misc_recomb 2070..2101 /note="Guid RNA cloning site" /note="color: #0000ff" stem_loop 2102..2244 /note="guide RNA scaffold 2.0" /note="color: #00ff00" promoter 2245..2610 /label=terminator /note="terminator" /note="color: #ff8080" misc_feature complement(2589..2610) /note="gRNA-R" /note="color: #0080ff; direction: LEFT" misc_feature 2616..2621 /note="BsaI Site" /note="color: #0080ff" misc_feature 2686..3359 /note="Ori" /note="color: #0080ff" misc_feature 3755..3774 /note="pTC14_F1 Primer" /note="color: #0080ff" ORIGIN 1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc 61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 181 aattaattct catgtttgac agcttatcat cgattagctt taatgcggta gtttatcaca 241 gttaaattgc taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat 301 cctcggcacc gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg 361 cctcttgcgg gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc 421 gctatatgcg ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt 481 tggccgccgc ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat 541 ggcgaccaca cccgtcctgt ggattctcta cgccggacgc atcgtggccg gcatcaccgg 601 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc 661 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc 721 cgggggactg ttgggcgcca tctccttaca tgcaccattc cttgcggcgg cggtgctcaa 781 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgccg 841 acccatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac 901 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc 961 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct 1021 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc 1081 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg 1141 ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct 1201 cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga 1261 cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat 1321 tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc 1381 atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg 1441 gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact 1501 ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttga 1561 tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg 1621 agaaaataaa atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag 1681 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca 1741 tgcctcctct agaggtctca ggacagaaat ctcaaaattc cggcagaaca attttgaatc 1801 tcgatccgta gaaaccagac ggtcattgtt ttagttccac cacgattata tttgaaattt 1861 acgtgagtgt gagtgagact tgcataagaa aataaaatct ttagttggga aaaaattcaa 1921 taatataaat gggcttgaga aggaagcgag ggataggcct ttttctaaaa taggcccatt 1981 taagctatta acaatcttca aaagtaccac agcgcttagg taaagaaagc agctgagttt 2041 atatatggtt agacacgaag tagtgattgg agacgagatc tagtctgagt cgaccgtctc 2101 tgttttagag ctaggccaac atgaggatca cccatgtctg cagggcctag caagttaaaa 2161 taaggctagt ccgttatcaa cttggccaac atgaggatca cccatgtctg cagggccaag 2221 tggcaccgag tcggtgcttt ttttggcaaa aattttcaga ttttttcttc atctgtagat 2281 ttctgggttt ttttttccgt ttcgtgaatc ataagtgaag ttttggatgc aaatctgcgc 2341 gaaaaaagtt ggacctgcaa tgagcttatt tagatagcta agacaaagtg attggtccgt 2401 tgtttcagtt ctgattgtca gagagtttgt ttcgagtcgg cgacaccaat gcgttttgtt 2461 aaccagattt cgggtaagaa atgtatcgag agtttgtttc cagacggcta catcattttc 2521 ttatgaaggg tgaaattaga tagaccaaag attgaaacac aacatttctt tcacaaaaat 2581 ataataaact tgatagcatt taggatcagc ccagggagac cctcgagcca cccatgacca 2641 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc 2701 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 2761 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 2821 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta 2881 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 2941 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 3001 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 3061 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg 3121 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 3181 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 3241 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 3301 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 3361 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct 3421 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa 3481 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg 3541 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc 3601 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct 3661 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct 3721 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca 3781 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc 3841 tggcagttcc ctactctcgc gtt //