LOCUS       pYPQ104_correct_        3623 bp ds-DNA     circular     02-MAR-2014
SOURCE      
  ORGANISM  
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     2676..2693
                     /dnas_title="M13-fwd"
                     /vntifkey="28"
                     /label=M13-fwd
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(155..175)
                     /dnas_title="M13-rev"
                     /vntifkey="28"
                     /label=M13-rev
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(135..156)
                     /dnas_title="T7"
                     /vntifkey="28"
                     /label=T7
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1464..2137
                     /dnas_title="pUC origin"
                     /vntifkey="21"
                     /label=pUC origin
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    387..1397
                     /dnas_title="SpnR"
                     /vntifkey="21"
                     /label=SpnR
                     /ApEinfo_fwdcolor="#f70938"
                     /ApEinfo_revcolor="#f70938"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    253..386
                     /dnas_title="Spn promoter"
                     /vntifkey="21"
                     /label=Spn promoter
                     /ApEinfo_fwdcolor="#2fec13"
                     /ApEinfo_revcolor="#2fec13"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2677..2692
                     /dnas_title="M13-F"
                     /vntifkey="21"
                     /label=M13-F
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(159..175)
                     /dnas_title="M13-R"
                     /vntifkey="21"
                     /label=M13-R
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    19..118
                     /dnas_title="attL2"
                     /vntifkey="21"
                     /label=attL2
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2695..2807
                     /dnas_title="attL5"
                     /vntifkey="21"
                     /label=attL5
                     /ApEinfo_fwdcolor="#c0c0c0"
                     /ApEinfo_revcolor="#c0c0c0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          1..2820
                     /dnas_title="pYPQ140"
     PCR_primer      2660..2683
                     /note="M13-F1"
ORIGIN      
        1 ctagtaaggg cgaattcgac ccagctttct tgtacaaagt tggcattata aaaaataatt
       61 gctcatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc
      121 atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
      181 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
      241 gcctcctctg gaccagccag gacagaaatg cctcgacttc gctgctgccc aaggttgccg
      301 ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg
      361 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
      421 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
      481 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
      541 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
      601 acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
      661 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
      721 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
      781 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
      841 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
      901 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
      961 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     1021 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     1081 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     1141 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     1201 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     1261 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     1321 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     1381 aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg
     1441 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     1501 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     1561 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     1621 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     1681 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     1741 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     1801 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     1861 tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     1921 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     1981 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     2041 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     2101 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     2161 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     2221 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     2281 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     2341 aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tcgccaggaa gagtttgtag
     2401 aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta
     2461 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc
     2521 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
     2581 ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcgt
     2641 taacgcttgc atggatgttt tcccagtcac gacgttgtaa aacgacggcc agtcttaagc
     2701 tcgggcccaa ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga
     2761 tgagcaatgc ttttttataa tgccaacttt gtatacaaaa gttgccccat ggcgttccct
//

