LOCUS       Exported                3688 bp ds-DNA     circular SYN 30-JUN-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pYPQ141A2.0
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jun 30, 2017 from SnapGene 3.3.4
            http://www.snapgene.com
COMMENT     LOCUS pYPQ104_correct_ 3623 bp ds-DNA circular 02-MAR-2014 SOURCE 
            ORGANISM COMMENT COMMENT ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..13
                     /label=For Left homology arm
                     /note="color: #------"
     terminator      19..324
                     /label=AtU6 promoter
                     /note="AtU6 promoter"
                     /note="color: #000000"
     -35_signal      260..270
                     /note="-35 signal"
                     /note="color: #00ff00; direction: RIGHT"
     TATA_signal     294..301
                     /note="TATA"
                     /note="color: #00ff00"
     misc_recomb     326..357
                     /note="Guid RNA cloning site"
                     /note="color: #0000ff; direction: RIGHT"
     stem_loop       358..500
                     /note="guide RNA scaffold 2.0"
                     /note="color: #0000ff; direction: RIGHT"
     promoter        501..866
                     /label=terminator
                     /note="terminator"
                     /note="color: #0000ff; direction: RIGHT"
     misc_feature    complement(845..866)
                     /note="gRNA-R"
                     /note="color: #0000ff; direction: LEFT"
     misc_feature    887..986
                     /label=attL2
                     /note="color: #0080ff"
     primer_bind     complement(1003..1024)
                     /label=T7
                     /note="color: #8080ff; direction: LEFT"
     primer_bind     complement(1023..1043)
                     /label=M13-rev
                     /note="color: #8080ff; direction: LEFT"
     misc_feature    complement(1027..1043)
                     /label=M13-R
                     /note="color: #0080ff; direction: LEFT"
     misc_feature    1121..1254
                     /label=Spn promoter
                     /note="color: #0080ff"
     misc_feature    1255..2265
                     /label=SpnR
                     /note="color: #0080ff"
     misc_feature    2332..3005
                     /label=pUC origin
                     /note="color: #0080ff"
     misc_feature    3528..3551
                     /note="M13-F1"
                     /note="color: #ff8080"
     primer_bind     3544..3561
                     /label=M13-fwd
                     /note="color: #00ff00; direction: RIGHT"
     misc_feature    3545..3560
                     /label=M13-F
                     /note="color: #0080ff"
     misc_feature    3563..3675
                     /label=attL5
                     /note="color: #0080ff"
ORIGIN
        1 ctagataacg caggatccta agaaatctca aaattccggc agaacaattt tgaatctcga
       61 tccgtagaaa ccagacggtc attgttttag ttccaccacg attatatttg aaatttacgt
      121 gagtgtgagt gagacttgca taagaaaata aaatctttag ttgggaaaaa attcaataat
      181 ataaatgggc ttgagaagga agcgagggat aggccttttt ctaaaatagg cccatttaag
      241 ctattaacaa tcttcaaaag taccacagcg cttaggtaaa gaaagcagct gagtttatat
      301 atggttagac acgaagtagt gattggagac gagatctagt ctgagtcgac cgtctctgtt
      361 ttagagctag gccaacatga ggatcaccca tgtctgcagg gcctagcaag ttaaaataag
      421 gctagtccgt tatcaacttg gccaacatga ggatcaccca tgtctgcagg gccaagtggc
      481 accgagtcgg tgcttttttt ggcaaaaatt ttcagatttt ttcttcatct gtagatttct
      541 gggttttttt ttccgtttcg tgaatcataa gtgaagtttt ggatgcaaat ctgcgcgaaa
      601 aaagttggac ctgcaatgag cttatttaga tagctaagac aaagtgattg gtccgttgtt
      661 tcagttctga ttgtcagaga gtttgtttcg agtcggcgac accaatgcgt tttgttaacc
      721 agatttcggg taagaaatgt atcgagagtt tgtttccaga cggctacatc attttcttat
      781 gaagggtgaa attagataga ccaaagattg aaacacaaca tttctttcac aaaaatataa
      841 taaacttgat agcatttagg atcagcaact agtaagggcg aattcgaccc agctttcttg
      901 tacaaagttg gcattataaa aaataattgc tcatcaattt gttgcaacga acaggtcact
      961 atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat
     1021 tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac
     1081 attgcacaag ataaaaatat atcatcatgc ctcctctgga ccagccagga cagaaatgcc
     1141 tcgacttcgc tgctgcccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca
     1201 cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc
     1261 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
     1321 ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag
     1381 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag
     1441 cagggcagtc gccctaaaac aaagttaaac atcatgaggg aagcggtgat cgccgaagta
     1501 tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg
     1561 gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat
     1621 ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac
     1681 cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc
     1741 attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt
     1801 ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt
     1861 gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg
     1921 gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc
     1981 ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg
     2041 ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc
     2101 gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag
     2161 gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt
     2221 gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataaccctc gagccaccca
     2281 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa
     2341 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     2401 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     2461 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     2521 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2581 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     2641 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2701 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc
     2761 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2821 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2881 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     2941 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     3001 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     3061 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     3121 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     3181 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg
     3241 cgtaccgctc gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct
     3301 tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc
     3361 gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca
     3421 ccgacaaaca acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt
     3481 gatgcctggc agttccctac tctcgcgtta acgcttgcat ggatgttttc ccagtcacga
     3541 cgttgtaaaa cgacggccag tcttaagctc gggcccaaat aatgatttta ttttgactga
     3601 tagtgacctg ttcgttgcaa caaattgatg agcaatgctt ttttataatg ccaactttgt
     3661 atacaaaagt tgccccatgg cgttccct
//
